Amino acid dipepetide frequency for Natronococcus occultus SP4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.728AlaAla: 12.728 ± 0.154
0.76AlaCys: 0.76 ± 0.025
9.839AlaAsp: 9.839 ± 0.118
9.08AlaGlu: 9.08 ± 0.101
3.828AlaPhe: 3.828 ± 0.056
8.911AlaGly: 8.911 ± 0.119
1.7AlaHis: 1.7 ± 0.04
5.537AlaIle: 5.537 ± 0.073
1.688AlaLys: 1.688 ± 0.039
10.044AlaLeu: 10.044 ± 0.117
2.034AlaMet: 2.034 ± 0.049
2.269AlaAsn: 2.269 ± 0.045
3.672AlaPro: 3.672 ± 0.062
1.982AlaGln: 1.982 ± 0.043
5.982AlaArg: 5.982 ± 0.076
5.123AlaSer: 5.123 ± 0.073
7.132AlaThr: 7.132 ± 0.083
10.478AlaVal: 10.478 ± 0.134
1.037AlaTrp: 1.037 ± 0.032
2.716AlaTyr: 2.716 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.582CysAla: 0.582 ± 0.026
0.121CysCys: 0.121 ± 0.01
0.579CysAsp: 0.579 ± 0.024
0.713CysGlu: 0.713 ± 0.03
0.189CysPhe: 0.189 ± 0.013
0.86CysGly: 0.86 ± 0.03
0.223CysHis: 0.223 ± 0.016
0.254CysIle: 0.254 ± 0.015
0.136CysLys: 0.136 ± 0.012
0.615CysLeu: 0.615 ± 0.022
0.114CysMet: 0.114 ± 0.01
0.193CysAsn: 0.193 ± 0.014
0.539CysPro: 0.539 ± 0.022
0.199CysGln: 0.199 ± 0.014
0.545CysArg: 0.545 ± 0.022
0.449CysSer: 0.449 ± 0.022
0.459CysThr: 0.459 ± 0.021
0.524CysVal: 0.524 ± 0.022
0.106CysTrp: 0.106 ± 0.009
0.246CysTyr: 0.246 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
9.745AspAla: 9.745 ± 0.11
0.733AspCys: 0.733 ± 0.027
8.533AspAsp: 8.533 ± 0.181
9.247AspGlu: 9.247 ± 0.144
1.649AspPhe: 1.649 ± 0.041
8.269AspGly: 8.269 ± 0.121
2.003AspHis: 2.003 ± 0.044
2.41AspIle: 2.41 ± 0.049
0.873AspLys: 0.873 ± 0.053
6.35AspLeu: 6.35 ± 0.084
1.105AspMet: 1.105 ± 0.03
1.127AspAsn: 1.127 ± 0.038
4.905AspPro: 4.905 ± 0.073
1.715AspGln: 1.715 ± 0.041
7.203AspArg: 7.203 ± 0.098
3.593AspSer: 3.593 ± 0.061
3.615AspThr: 3.615 ± 0.057
8.546AspVal: 8.546 ± 0.099
1.047AspTrp: 1.047 ± 0.032
1.857AspTyr: 1.857 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
8.916GluAla: 8.916 ± 0.114
0.541GluCys: 0.541 ± 0.024
8.591GluAsp: 8.591 ± 0.119
7.932GluGlu: 7.932 ± 0.127
3.267GluPhe: 3.267 ± 0.052
5.827GluGly: 5.827 ± 0.077
1.956GluHis: 1.956 ± 0.042
4.659GluIle: 4.659 ± 0.08
1.902GluLys: 1.902 ± 0.048
9.5GluLeu: 9.5 ± 0.113
1.872GluMet: 1.872 ± 0.035
2.513GluAsn: 2.513 ± 0.061
4.974GluPro: 4.974 ± 0.089
2.697GluGln: 2.697 ± 0.053
8.618GluArg: 8.618 ± 0.114
5.338GluSer: 5.338 ± 0.082
7.303GluThr: 7.303 ± 0.086
5.345GluVal: 5.345 ± 0.079
1.243GluTrp: 1.243 ± 0.033
3.083GluTyr: 3.083 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.595PheAla: 3.595 ± 0.061
0.272PheCys: 0.272 ± 0.015
3.121PheAsp: 3.121 ± 0.055
3.376PheGlu: 3.376 ± 0.052
0.996PhePhe: 0.996 ± 0.037
3.138PheGly: 3.138 ± 0.056
0.647PheHis: 0.647 ± 0.024
1.001PheIle: 1.001 ± 0.028
0.424PheLys: 0.424 ± 0.021
2.765PheLeu: 2.765 ± 0.057
0.516PheMet: 0.516 ± 0.023
0.617PheAsn: 0.617 ± 0.027
1.337PhePro: 1.337 ± 0.035
0.704PheGln: 0.704 ± 0.023
1.679PheArg: 1.679 ± 0.04
1.542PheSer: 1.542 ± 0.037
1.725PheThr: 1.725 ± 0.039
3.239PheVal: 3.239 ± 0.05
0.401PheTrp: 0.401 ± 0.018
0.856PheTyr: 0.856 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
8.064GlyAla: 8.064 ± 0.113
0.784GlyCys: 0.784 ± 0.027
6.268GlyAsp: 6.268 ± 0.095
7.548GlyGlu: 7.548 ± 0.085
3.048GlyPhe: 3.048 ± 0.054
6.604GlyGly: 6.604 ± 0.113
1.642GlyHis: 1.642 ± 0.042
4.44GlyIle: 4.44 ± 0.062
1.729GlyLys: 1.729 ± 0.042
7.663GlyLeu: 7.663 ± 0.092
1.633GlyMet: 1.633 ± 0.04
1.746GlyAsn: 1.746 ± 0.042
3.26GlyPro: 3.26 ± 0.051
1.863GlyGln: 1.863 ± 0.041
4.798GlyArg: 4.798 ± 0.07
5.069GlySer: 5.069 ± 0.071
5.74GlyThr: 5.74 ± 0.079
7.25GlyVal: 7.25 ± 0.092
1.182GlyTrp: 1.182 ± 0.03
2.808GlyTyr: 2.808 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
1.937HisAla: 1.937 ± 0.045
0.211HisCys: 0.211 ± 0.013
1.838HisAsp: 1.838 ± 0.038
1.918HisGlu: 1.918 ± 0.041
0.503HisPhe: 0.503 ± 0.021
1.979HisGly: 1.979 ± 0.044
0.521HisHis: 0.521 ± 0.022
0.632HisIle: 0.632 ± 0.026
0.307HisLys: 0.307 ± 0.016
1.679HisLeu: 1.679 ± 0.034
0.279HisMet: 0.279 ± 0.015
0.457HisAsn: 0.457 ± 0.021
1.222HisPro: 1.222 ± 0.036
0.485HisGln: 0.485 ± 0.022
1.306HisArg: 1.306 ± 0.038
0.879HisSer: 0.879 ± 0.028
1.009HisThr: 1.009 ± 0.028
1.831HisVal: 1.831 ± 0.046
0.26HisTrp: 0.26 ± 0.015
0.563HisTyr: 0.563 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.166IleAla: 5.166 ± 0.08
0.313IleCys: 0.313 ± 0.018
4.329IleAsp: 4.329 ± 0.061
4.71IleGlu: 4.71 ± 0.072
1.073IlePhe: 1.073 ± 0.035
4.207IleGly: 4.207 ± 0.069
0.822IleHis: 0.822 ± 0.026
1.356IleIle: 1.356 ± 0.042
0.696IleLys: 0.696 ± 0.029
2.986IleLeu: 2.986 ± 0.065
0.546IleMet: 0.546 ± 0.021
0.966IleAsn: 0.966 ± 0.029
2.072IlePro: 2.072 ± 0.046
0.989IleGln: 0.989 ± 0.032
2.473IleArg: 2.473 ± 0.049
2.069IleSer: 2.069 ± 0.051
2.406IleThr: 2.406 ± 0.044
4.31IleVal: 4.31 ± 0.061
0.353IleTrp: 0.353 ± 0.019
1.035IleTyr: 1.035 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
1.577LysAla: 1.577 ± 0.038
0.104LysCys: 0.104 ± 0.009
1.003LysAsp: 1.003 ± 0.041
1.288LysGlu: 1.288 ± 0.034
0.45LysPhe: 0.45 ± 0.017
1.253LysGly: 1.253 ± 0.038
0.495LysHis: 0.495 ± 0.019
0.793LysIle: 0.793 ± 0.028
0.571LysLys: 0.571 ± 0.044
1.576LysLeu: 1.576 ± 0.039
0.34LysMet: 0.34 ± 0.016
0.515LysAsn: 0.515 ± 0.026
0.898LysPro: 0.898 ± 0.03
0.666LysGln: 0.666 ± 0.026
1.676LysArg: 1.676 ± 0.04
1.014LysSer: 1.014 ± 0.032
1.186LysThr: 1.186 ± 0.035
1.063LysVal: 1.063 ± 0.032
0.216LysTrp: 0.216 ± 0.014
0.575LysTyr: 0.575 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
11.364LeuAla: 11.364 ± 0.139
0.69LeuCys: 0.69 ± 0.024
6.142LeuAsp: 6.142 ± 0.076
11.561LeuGlu: 11.561 ± 0.124
3.118LeuPhe: 3.118 ± 0.061
7.671LeuGly: 7.671 ± 0.106
1.441LeuHis: 1.441 ± 0.037
3.131LeuIle: 3.131 ± 0.058
1.365LeuLys: 1.365 ± 0.039
8.795LeuLeu: 8.795 ± 0.115
1.196LeuMet: 1.196 ± 0.033
1.638LeuAsn: 1.638 ± 0.042
4.063LeuPro: 4.063 ± 0.058
2.047LeuGln: 2.047 ± 0.049
5.295LeuArg: 5.295 ± 0.069
5.455LeuSer: 5.455 ± 0.085
4.984LeuThr: 4.984 ± 0.073
8.221LeuVal: 8.221 ± 0.126
0.89LeuTrp: 0.89 ± 0.033
2.237LeuTyr: 2.237 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
1.731MetAla: 1.731 ± 0.043
0.114MetCys: 0.114 ± 0.01
1.117MetAsp: 1.117 ± 0.038
1.131MetGlu: 1.131 ± 0.031
0.502MetPhe: 0.502 ± 0.025
1.415MetGly: 1.415 ± 0.038
0.331MetHis: 0.331 ± 0.017
0.77MetIle: 0.77 ± 0.028
0.415MetLys: 0.415 ± 0.019
1.485MetLeu: 1.485 ± 0.037
0.321MetMet: 0.321 ± 0.018
0.575MetAsn: 0.575 ± 0.024
0.75MetPro: 0.75 ± 0.025
0.458MetGln: 0.458 ± 0.02
1.007MetArg: 1.007 ± 0.027
1.486MetSer: 1.486 ± 0.036
1.546MetThr: 1.546 ± 0.037
1.227MetVal: 1.227 ± 0.03
0.176MetTrp: 0.176 ± 0.013
0.414MetTyr: 0.414 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.435AsnAla: 2.435 ± 0.046
0.246AsnCys: 0.246 ± 0.015
1.654AsnAsp: 1.654 ± 0.05
1.873AsnGlu: 1.873 ± 0.042
0.607AsnPhe: 0.607 ± 0.023
1.94AsnGly: 1.94 ± 0.051
0.503AsnHis: 0.503 ± 0.02
0.765AsnIle: 0.765 ± 0.029
0.419AsnLys: 0.419 ± 0.022
1.725AsnLeu: 1.725 ± 0.042
0.414AsnMet: 0.414 ± 0.018
0.501AsnAsn: 0.501 ± 0.024
1.425AsnPro: 1.425 ± 0.042
0.615AsnGln: 0.615 ± 0.024
1.64AsnArg: 1.64 ± 0.037
0.889AsnSer: 0.889 ± 0.029
1.197AsnThr: 1.197 ± 0.029
2.173AsnVal: 2.173 ± 0.046
0.289AsnTrp: 0.289 ± 0.017
0.653AsnTyr: 0.653 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
4.563ProAla: 4.563 ± 0.069
0.249ProCys: 0.249 ± 0.015
4.821ProAsp: 4.821 ± 0.078
5.128ProGlu: 5.128 ± 0.07
1.515ProPhe: 1.515 ± 0.041
3.677ProGly: 3.677 ± 0.06
0.881ProHis: 0.881 ± 0.026
2.396ProIle: 2.396 ± 0.043
0.871ProLys: 0.871 ± 0.029
3.657ProLeu: 3.657 ± 0.062
0.816ProMet: 0.816 ± 0.025
1.182ProAsn: 1.182 ± 0.032
2.064ProPro: 2.064 ± 0.046
0.94ProGln: 0.94 ± 0.028
2.326ProArg: 2.326 ± 0.048
2.569ProSer: 2.569 ± 0.045
3.219ProThr: 3.219 ± 0.059
3.974ProVal: 3.974 ± 0.063
0.526ProTrp: 0.526 ± 0.021
1.134ProTyr: 1.134 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.19GlnAla: 2.19 ± 0.051
0.166GlnCys: 0.166 ± 0.011
1.26GlnAsp: 1.26 ± 0.032
1.851GlnGlu: 1.851 ± 0.046
0.958GlnPhe: 0.958 ± 0.029
1.557GlnGly: 1.557 ± 0.037
0.517GlnHis: 0.517 ± 0.02
1.054GlnIle: 1.054 ± 0.037
0.491GlnLys: 0.491 ± 0.022
2.635GlnLeu: 2.635 ± 0.046
0.502GlnMet: 0.502 ± 0.021
0.691GlnAsn: 0.691 ± 0.023
1.157GlnPro: 1.157 ± 0.025
0.953GlnGln: 0.953 ± 0.033
1.901GlnArg: 1.901 ± 0.044
1.387GlnSer: 1.387 ± 0.042
1.447GlnThr: 1.447 ± 0.033
1.588GlnVal: 1.588 ± 0.037
0.347GlnTrp: 0.347 ± 0.019
0.844GlnTyr: 0.844 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
6.235ArgAla: 6.235 ± 0.076
0.518ArgCys: 0.518 ± 0.023
4.612ArgAsp: 4.612 ± 0.07
7.614ArgGlu: 7.614 ± 0.095
2.271ArgPhe: 2.271 ± 0.044
4.377ArgGly: 4.377 ± 0.059
1.237ArgHis: 1.237 ± 0.034
3.186ArgIle: 3.186 ± 0.057
1.408ArgLys: 1.408 ± 0.038
6.364ArgLeu: 6.364 ± 0.083
1.3ArgMet: 1.3 ± 0.038
1.594ArgAsn: 1.594 ± 0.037
2.582ArgPro: 2.582 ± 0.05
1.768ArgGln: 1.768 ± 0.04
5.252ArgArg: 5.252 ± 0.083
3.953ArgSer: 3.953 ± 0.061
4.33ArgThr: 4.33 ± 0.068
5.015ArgVal: 5.015 ± 0.077
0.831ArgTrp: 0.831 ± 0.029
2.11ArgTyr: 2.11 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
4.886SerAla: 4.886 ± 0.068
0.377SerCys: 0.377 ± 0.02
4.132SerAsp: 4.132 ± 0.083
4.658SerGlu: 4.658 ± 0.084
1.817SerPhe: 1.817 ± 0.04
4.751SerGly: 4.751 ± 0.074
1.069SerHis: 1.069 ± 0.034
2.595SerIle: 2.595 ± 0.05
1.115SerLys: 1.115 ± 0.032
4.821SerLeu: 4.821 ± 0.076
1.1SerMet: 1.1 ± 0.03
1.272SerAsn: 1.272 ± 0.037
2.488SerPro: 2.488 ± 0.049
1.308SerGln: 1.308 ± 0.034
3.41SerArg: 3.41 ± 0.055
3.227SerSer: 3.227 ± 0.079
3.357SerThr: 3.357 ± 0.061
4.8SerVal: 4.8 ± 0.064
0.63SerTrp: 0.63 ± 0.023
1.496SerTyr: 1.496 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
6.848ThrAla: 6.848 ± 0.09
0.418ThrCys: 0.418 ± 0.018
5.781ThrAsp: 5.781 ± 0.08
5.051ThrGlu: 5.051 ± 0.065
2.013ThrPhe: 2.013 ± 0.043
5.47ThrGly: 5.47 ± 0.082
1.23ThrHis: 1.23 ± 0.034
3.321ThrIle: 3.321 ± 0.054
1.005ThrLys: 1.005 ± 0.028
5.576ThrLeu: 5.576 ± 0.067
1.028ThrMet: 1.028 ± 0.031
1.446ThrAsn: 1.446 ± 0.036
3.093ThrPro: 3.093 ± 0.056
1.307ThrGln: 1.307 ± 0.031
3.18ThrArg: 3.18 ± 0.05
2.829ThrSer: 2.829 ± 0.051
4.081ThrThr: 4.081 ± 0.068
6.747ThrVal: 6.747 ± 0.089
0.567ThrTrp: 0.567 ± 0.021
1.628ThrTyr: 1.628 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
10.146ValAla: 10.146 ± 0.12
0.656ValCys: 0.656 ± 0.023
7.453ValAsp: 7.453 ± 0.086
7.693ValGlu: 7.693 ± 0.1
2.819ValPhe: 2.819 ± 0.061
7.892ValGly: 7.892 ± 0.102
1.719ValHis: 1.719 ± 0.035
3.203ValIle: 3.203 ± 0.065
1.156ValLys: 1.156 ± 0.034
9.198ValLeu: 9.198 ± 0.096
1.25ValMet: 1.25 ± 0.031
1.696ValAsn: 1.696 ± 0.044
4.312ValPro: 4.312 ± 0.064
1.753ValGln: 1.753 ± 0.035
5.397ValArg: 5.397 ± 0.067
4.635ValSer: 4.635 ± 0.064
5.572ValThr: 5.572 ± 0.076
8.8ValVal: 8.8 ± 0.122
0.823ValTrp: 0.823 ± 0.024
2.116ValTyr: 2.116 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.93TrpAla: 0.93 ± 0.027
0.089TrpCys: 0.089 ± 0.01
0.761TrpAsp: 0.761 ± 0.024
0.968TrpGlu: 0.968 ± 0.031
0.473TrpPhe: 0.473 ± 0.019
0.847TrpGly: 0.847 ± 0.022
0.237TrpHis: 0.237 ± 0.014
0.609TrpIle: 0.609 ± 0.025
0.278TrpLys: 0.278 ± 0.017
1.287TrpLeu: 1.287 ± 0.033
0.23TrpMet: 0.23 ± 0.014
0.338TrpAsn: 0.338 ± 0.017
0.437TrpPro: 0.437 ± 0.021
0.368TrpGln: 0.368 ± 0.019
0.828TrpArg: 0.828 ± 0.025
0.631TrpSer: 0.631 ± 0.026
0.815TrpThr: 0.815 ± 0.024
0.795TrpVal: 0.795 ± 0.027
0.213TrpTrp: 0.213 ± 0.015
0.378TrpTyr: 0.378 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.899TyrAla: 2.899 ± 0.051
0.284TyrCys: 0.284 ± 0.018
2.691TyrAsp: 2.691 ± 0.048
2.74TyrGlu: 2.74 ± 0.053
0.856TyrPhe: 0.856 ± 0.027
2.492TyrGly: 2.492 ± 0.051
0.644TyrHis: 0.644 ± 0.023
0.767TyrIle: 0.767 ± 0.025
0.41TyrLys: 0.41 ± 0.022
2.582TyrLeu: 2.582 ± 0.056
0.379TyrMet: 0.379 ± 0.019
0.628TyrAsn: 0.628 ± 0.025
1.255TyrPro: 1.255 ± 0.039
0.678TyrGln: 0.678 ± 0.022
2.04TyrArg: 2.04 ± 0.042
1.171TyrSer: 1.171 ± 0.031
1.495TyrThr: 1.495 ± 0.041
2.362TyrVal: 2.362 ± 0.046
0.374TyrTrp: 0.374 ± 0.021
0.885TyrTyr: 0.885 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4153 proteins (1196774 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski