Streptococcus satellite phage Javan245

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHQ4|A0A4D5ZHQ4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan245 OX=2558589 GN=JavanS245_0005 PE=4 SV=1
MM1 pKa = 7.09EE2 pKa = 4.26QATSRR7 pKa = 11.84LNNVAVEE14 pKa = 4.05AVEE17 pKa = 4.04VLTAIMNDD25 pKa = 3.64PTISPYY31 pKa = 10.94ARR33 pKa = 11.84QQSARR38 pKa = 11.84TILEE42 pKa = 4.01FAYY45 pKa = 9.67KK46 pKa = 10.12AYY48 pKa = 9.89EE49 pKa = 3.87NEE51 pKa = 4.25AIIEE55 pKa = 4.08KK56 pKa = 10.56LEE58 pKa = 3.9EE59 pKa = 4.52LEE61 pKa = 4.34TVISIDD67 pKa = 4.39DD68 pKa = 3.56KK69 pKa = 11.82GII71 pKa = 3.54

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZI71|A0A4D5ZI71_9VIRU Integrase OS=Streptococcus satellite phage Javan245 OX=2558589 GN=JavanS245_0011 PE=3 SV=1
MM1 pKa = 7.34ATFLKK6 pKa = 10.39KK7 pKa = 9.96VRR9 pKa = 11.84KK10 pKa = 8.56NKK12 pKa = 9.66AYY14 pKa = 9.69KK15 pKa = 10.18RR16 pKa = 11.84LFQAVPRR23 pKa = 11.84HH24 pKa = 5.36SKK26 pKa = 10.65EE27 pKa = 3.81FLQMSWFSPLFYY39 pKa = 10.96

Molecular weight: 4.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

1477

39

388

134.3

15.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.281 ± 0.737

0.542 ± 0.144

5.958 ± 0.943

6.974 ± 0.738

4.942 ± 0.558

4.062 ± 0.394

1.49 ± 0.426

9.005 ± 0.917

8.666 ± 0.718

9.614 ± 0.485

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.372

6.703 ± 0.673

3.114 ± 0.276

3.182 ± 0.47

4.604 ± 0.379

6.229 ± 0.304

6.026 ± 0.507

6.026 ± 0.467

0.88 ± 0.21

4.401 ± 0.539
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski