Amphimedon queenslandica (Sponge)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Porifera; Demospongiae; Heteroscleromorpha; Haplosclerida; Niphatidae; Amphimedon

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43437 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7U971|A0A1X7U971_AMPQE Protein kinase domain-containing protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
MM1 pKa = 7.21WKK3 pKa = 10.22LVAGMNLTIQDD14 pKa = 4.28LEE16 pKa = 4.29EE17 pKa = 4.36TLDD20 pKa = 4.0GLPTAQTCFFQLRR33 pKa = 11.84LPPYY37 pKa = 9.85SSQAVLAEE45 pKa = 3.92RR46 pKa = 11.84LLYY49 pKa = 10.42AIRR52 pKa = 11.84HH53 pKa = 5.61CRR55 pKa = 11.84SIDD58 pKa = 3.09MDD60 pKa = 3.54NYY62 pKa = 9.27MLRR65 pKa = 11.84RR66 pKa = 11.84NNSMVSGGEE75 pKa = 3.59IDD77 pKa = 4.52SDD79 pKa = 4.1YY80 pKa = 11.58SDD82 pKa = 4.86YY83 pKa = 11.57YY84 pKa = 11.44YY85 pKa = 11.15DD86 pKa = 6.34DD87 pKa = 6.35DD88 pKa = 7.45DD89 pKa = 7.58DD90 pKa = 7.55DD91 pKa = 7.44DD92 pKa = 7.44DD93 pKa = 7.46DD94 pKa = 7.53DD95 pKa = 7.61DD96 pKa = 7.67DD97 pKa = 6.0IFDD100 pKa = 3.65

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7SVE9|A0A1X7SVE9_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=3 SV=1
SS1 pKa = 5.5TVSRR5 pKa = 11.84STIGWSTIGRR15 pKa = 11.84STVGRR20 pKa = 11.84STIGRR25 pKa = 11.84STISRR30 pKa = 11.84STIGWSTVGRR40 pKa = 11.84STVGRR45 pKa = 11.84STVGRR50 pKa = 11.84STVGLSTINWSTIGRR65 pKa = 11.84STIGRR70 pKa = 11.84STIGRR75 pKa = 11.84STIGRR80 pKa = 11.84STIGRR85 pKa = 11.84PTIGWSTIGWSTIGWSTINQSIISRR110 pKa = 11.84FTIGG114 pKa = 3.67

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43437

0

43437

14716634

7

18893

338.8

37.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.39 ± 0.011

2.262 ± 0.014

5.466 ± 0.011

6.302 ± 0.019

3.783 ± 0.01

5.685 ± 0.016

2.493 ± 0.007

6.344 ± 0.012

5.99 ± 0.022

9.614 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.101 ± 0.006

4.97 ± 0.014

4.768 ± 0.016

3.882 ± 0.01

4.425 ± 0.012

9.133 ± 0.021

6.34 ± 0.019

6.434 ± 0.011

1.087 ± 0.004

3.47 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski