Amino acid dipepetide frequency for Brevibacterium casei S18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.384AlaAla: 18.384 ± 0.188	0.753AlaCys: 0.753 ± 0.027	8.749AlaAsp: 8.749 ± 0.105	10.164AlaGlu: 10.164 ± 0.124	3.647AlaPhe: 3.647 ± 0.067	12.215AlaGly: 12.215 ± 0.118	2.408AlaHis: 2.408 ± 0.059	5.768AlaIle: 5.768 ± 0.08	3.46AlaLys: 3.46 ± 0.071	12.389AlaLeu: 12.389 ± 0.128	2.664AlaMet: 2.664 ± 0.052	2.371AlaAsn: 2.371 ± 0.053	5.694AlaPro: 5.694 ± 0.085	3.569AlaGln: 3.569 ± 0.061	7.878AlaArg: 7.878 ± 0.095	6.774AlaSer: 6.774 ± 0.078	6.79AlaThr: 6.79 ± 0.071	10.343AlaVal: 10.343 ± 0.094	1.61AlaTrp: 1.61 ± 0.042	2.103AlaTyr: 2.103 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.708CysAla: 0.708 ± 0.027	0.063CysCys: 0.063 ± 0.008	0.303CysAsp: 0.303 ± 0.018	0.306CysGlu: 0.306 ± 0.016	0.207CysPhe: 0.207 ± 0.016	0.633CysGly: 0.633 ± 0.029	0.135CysHis: 0.135 ± 0.012	0.21CysIle: 0.21 ± 0.013	0.081CysLys: 0.081 ± 0.01	0.594CysLeu: 0.594 ± 0.023	0.077CysMet: 0.077 ± 0.009	0.11CysAsn: 0.11 ± 0.008	0.344CysPro: 0.344 ± 0.017	0.129CysGln: 0.129 ± 0.012	0.398CysArg: 0.398 ± 0.022	0.329CysSer: 0.329 ± 0.019	0.408CysThr: 0.408 ± 0.019	0.421CysVal: 0.421 ± 0.019	0.068CysTrp: 0.068 ± 0.008	0.12CysTyr: 0.12 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.883AspAla: 7.883 ± 0.096	0.331AspCys: 0.331 ± 0.019	4.709AspAsp: 4.709 ± 0.076	4.861AspGlu: 4.861 ± 0.073	2.293AspPhe: 2.293 ± 0.051	5.668AspGly: 5.668 ± 0.086	1.478AspHis: 1.478 ± 0.038	2.802AspIle: 2.802 ± 0.051	1.161AspLys: 1.161 ± 0.037	6.866AspLeu: 6.866 ± 0.093	0.976AspMet: 0.976 ± 0.027	1.042AspAsn: 1.042 ± 0.031	4.606AspPro: 4.606 ± 0.074	1.539AspGln: 1.539 ± 0.038	4.844AspArg: 4.844 ± 0.072	3.682AspSer: 3.682 ± 0.066	3.289AspThr: 3.289 ± 0.06	5.178AspVal: 5.178 ± 0.079	0.896AspTrp: 0.896 ± 0.028	1.426AspTyr: 1.426 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	8.331GluAla: 8.331 ± 0.113	0.271GluCys: 0.271 ± 0.019	3.544GluAsp: 3.544 ± 0.067	3.694GluGlu: 3.694 ± 0.075	2.022GluPhe: 2.022 ± 0.05	3.992GluGly: 3.992 ± 0.056	1.44GluHis: 1.44 ± 0.04	3.294GluIle: 3.294 ± 0.06	1.851GluLys: 1.851 ± 0.055	6.38GluLeu: 6.38 ± 0.088	1.078GluMet: 1.078 ± 0.031	1.472GluAsn: 1.472 ± 0.039	2.832GluPro: 2.832 ± 0.05	1.896GluGln: 1.896 ± 0.042	4.545GluArg: 4.545 ± 0.068	3.728GluSer: 3.728 ± 0.059	3.924GluThr: 3.924 ± 0.071	5.468GluVal: 5.468 ± 0.086	0.793GluTrp: 0.793 ± 0.029	1.146GluTyr: 1.146 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.615PheAla: 4.615 ± 0.086	0.203PheCys: 0.203 ± 0.015	2.622PheAsp: 2.622 ± 0.047	1.625PheGlu: 1.625 ± 0.041	1.17PhePhe: 1.17 ± 0.041	3.474PheGly: 3.474 ± 0.072	0.578PheHis: 0.578 ± 0.025	1.417PheIle: 1.417 ± 0.045	0.567PheLys: 0.567 ± 0.023	2.899PheLeu: 2.899 ± 0.061	0.504PheMet: 0.504 ± 0.022	0.696PheAsn: 0.696 ± 0.027	1.402PhePro: 1.402 ± 0.039	0.64PheGln: 0.64 ± 0.022	1.63PheArg: 1.63 ± 0.035	2.112PheSer: 2.112 ± 0.043	2.25PheThr: 2.25 ± 0.047	2.835PheVal: 2.835 ± 0.065	0.452PheTrp: 0.452 ± 0.021	0.651PheTyr: 0.651 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	9.915GlyAla: 9.915 ± 0.114	0.565GlyCys: 0.565 ± 0.024	5.237GlyAsp: 5.237 ± 0.085	5.375GlyGlu: 5.375 ± 0.08	3.369GlyPhe: 3.369 ± 0.053	7.699GlyGly: 7.699 ± 0.132	1.877GlyHis: 1.877 ± 0.048	5.06GlyIle: 5.06 ± 0.075	2.339GlyLys: 2.339 ± 0.053	9.3GlyLeu: 9.3 ± 0.107	2.035GlyMet: 2.035 ± 0.044	1.78GlyAsn: 1.78 ± 0.045	4.167GlyPro: 4.167 ± 0.077	2.642GlyGln: 2.642 ± 0.062	6.206GlyArg: 6.206 ± 0.085	5.913GlySer: 5.913 ± 0.097	6.023GlyThr: 6.023 ± 0.083	7.109GlyVal: 7.109 ± 0.083	1.457GlyTrp: 1.457 ± 0.04	2.163GlyTyr: 2.163 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.211HisAla: 2.211 ± 0.051	0.13HisCys: 0.13 ± 0.011	1.298HisAsp: 1.298 ± 0.036	1.08HisGlu: 1.08 ± 0.031	0.608HisPhe: 0.608 ± 0.025	1.891HisGly: 1.891 ± 0.04	0.522HisHis: 0.522 ± 0.025	0.837HisIle: 0.837 ± 0.03	0.329HisLys: 0.329 ± 0.018	2.06HisLeu: 2.06 ± 0.048	0.334HisMet: 0.334 ± 0.017	0.347HisAsn: 0.347 ± 0.018	1.614HisPro: 1.614 ± 0.046	0.504HisGln: 0.504 ± 0.021	1.595HisArg: 1.595 ± 0.042	1.098HisSer: 1.098 ± 0.031	1.118HisThr: 1.118 ± 0.032	1.476HisVal: 1.476 ± 0.046	0.257HisTrp: 0.257 ± 0.015	0.39HisTyr: 0.39 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	7.189IleAla: 7.189 ± 0.088	0.314IleCys: 0.314 ± 0.015	3.952IleAsp: 3.952 ± 0.058	2.848IleGlu: 2.848 ± 0.052	1.321IlePhe: 1.321 ± 0.043	5.106IleGly: 5.106 ± 0.083	0.807IleHis: 0.807 ± 0.028	2.393IleIle: 2.393 ± 0.056	0.885IleLys: 0.885 ± 0.038	4.087IleLeu: 4.087 ± 0.079	0.779IleMet: 0.779 ± 0.028	1.057IleAsn: 1.057 ± 0.035	2.53IlePro: 2.53 ± 0.053	0.838IleGln: 0.838 ± 0.026	2.843IleArg: 2.843 ± 0.046	2.58IleSer: 2.58 ± 0.048	3.101IleThr: 3.101 ± 0.065	5.005IleVal: 5.005 ± 0.082	0.452IleTrp: 0.452 ± 0.02	0.764IleTyr: 0.764 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.871LysAla: 2.871 ± 0.059	0.097LysCys: 0.097 ± 0.01	1.554LysAsp: 1.554 ± 0.047	1.291LysGlu: 1.291 ± 0.043	0.648LysPhe: 0.648 ± 0.027	1.832LysGly: 1.832 ± 0.047	0.481LysHis: 0.481 ± 0.019	1.054LysIle: 1.054 ± 0.036	1.028LysLys: 1.028 ± 0.043	1.959LysLeu: 1.959 ± 0.047	0.451LysMet: 0.451 ± 0.019	0.641LysAsn: 0.641 ± 0.025	1.277LysPro: 1.277 ± 0.039	0.732LysGln: 0.732 ± 0.027	1.74LysArg: 1.74 ± 0.046	1.46LysSer: 1.46 ± 0.043	1.424LysThr: 1.424 ± 0.044	1.853LysVal: 1.853 ± 0.054	0.314LysTrp: 0.314 ± 0.017	0.582LysTyr: 0.582 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.065LeuAla: 13.065 ± 0.149	0.59LeuCys: 0.59 ± 0.026	6.717LeuAsp: 6.717 ± 0.087	5.053LeuGlu: 5.053 ± 0.073	2.954LeuPhe: 2.954 ± 0.076	9.767LeuGly: 9.767 ± 0.104	1.766LeuHis: 1.766 ± 0.042	4.999LeuIle: 4.999 ± 0.087	1.882LeuLys: 1.882 ± 0.049	9.561LeuLeu: 9.561 ± 0.127	1.651LeuMet: 1.651 ± 0.037	1.837LeuAsn: 1.837 ± 0.04	5.365LeuPro: 5.365 ± 0.083	2.035LeuGln: 2.035 ± 0.048	6.766LeuArg: 6.766 ± 0.085	6.295LeuSer: 6.295 ± 0.077	6.786LeuThr: 6.786 ± 0.077	8.481LeuVal: 8.481 ± 0.102	1.109LeuTrp: 1.109 ± 0.034	1.439LeuTyr: 1.439 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	2.299MetAla: 2.299 ± 0.042	0.126MetCys: 0.126 ± 0.01	0.913MetAsp: 0.913 ± 0.029	0.779MetGlu: 0.779 ± 0.026	0.597MetPhe: 0.597 ± 0.025	1.402MetGly: 1.402 ± 0.035	0.375MetHis: 0.375 ± 0.02	0.997MetIle: 0.997 ± 0.034	0.518MetLys: 0.518 ± 0.023	1.835MetLeu: 1.835 ± 0.042	0.377MetMet: 0.377 ± 0.022	0.513MetAsn: 0.513 ± 0.021	1.117MetPro: 1.117 ± 0.038	0.466MetGln: 0.466 ± 0.024	1.451MetArg: 1.451 ± 0.04	1.686MetSer: 1.686 ± 0.04	1.697MetThr: 1.697 ± 0.04	1.431MetVal: 1.431 ± 0.039	0.22MetTrp: 0.22 ± 0.015	0.336MetTyr: 0.336 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.411AsnAla: 2.411 ± 0.053	0.128AsnCys: 0.128 ± 0.011	1.214AsnAsp: 1.214 ± 0.039	1.05AsnGlu: 1.05 ± 0.034	0.647AsnPhe: 0.647 ± 0.024	1.823AsnGly: 1.823 ± 0.046	0.389AsnHis: 0.389 ± 0.018	0.925AsnIle: 0.925 ± 0.032	0.419AsnLys: 0.419 ± 0.019	1.978AsnLeu: 1.978 ± 0.046	0.363AsnMet: 0.363 ± 0.019	0.453AsnAsn: 0.453 ± 0.021	1.651AsnPro: 1.651 ± 0.034	0.574AsnGln: 0.574 ± 0.027	1.451AsnArg: 1.451 ± 0.04	1.207AsnSer: 1.207 ± 0.033	1.159AsnThr: 1.159 ± 0.033	1.535AsnVal: 1.535 ± 0.041	0.291AsnTrp: 0.291 ± 0.016	0.491AsnTyr: 0.491 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	6.822ProAla: 6.822 ± 0.091	0.196ProCys: 0.196 ± 0.014	3.987ProAsp: 3.987 ± 0.073	4.367ProGlu: 4.367 ± 0.064	1.658ProPhe: 1.658 ± 0.046	5.493ProGly: 5.493 ± 0.09	1.068ProHis: 1.068 ± 0.032	2.184ProIle: 2.184 ± 0.052	1.307ProLys: 1.307 ± 0.037	4.431ProLeu: 4.431 ± 0.066	0.99ProMet: 0.99 ± 0.033	1.127ProAsn: 1.127 ± 0.03	2.215ProPro: 2.215 ± 0.064	1.597ProGln: 1.597 ± 0.048	3.223ProArg: 3.223 ± 0.059	3.007ProSer: 3.007 ± 0.055	3.546ProThr: 3.546 ± 0.06	4.59ProVal: 4.59 ± 0.06	0.77ProTrp: 0.77 ± 0.03	0.991ProTyr: 0.991 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.42GlnAla: 3.42 ± 0.054	0.113GlnCys: 0.113 ± 0.009	1.137GlnAsp: 1.137 ± 0.031	1.232GlnGlu: 1.232 ± 0.032	0.749GlnPhe: 0.749 ± 0.029	1.974GlnGly: 1.974 ± 0.047	0.519GlnHis: 0.519 ± 0.025	1.391GlnIle: 1.391 ± 0.037	0.708GlnLys: 0.708 ± 0.03	2.724GlnLeu: 2.724 ± 0.05	0.602GlnMet: 0.602 ± 0.023	0.594GlnAsn: 0.594 ± 0.025	1.51GlnPro: 1.51 ± 0.045	0.947GlnGln: 0.947 ± 0.044	2.045GlnArg: 2.045 ± 0.049	1.542GlnSer: 1.542 ± 0.04	1.52GlnThr: 1.52 ± 0.036	2.219GlnVal: 2.219 ± 0.048	0.442GlnTrp: 0.442 ± 0.022	0.566GlnTyr: 0.566 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	7.708ArgAla: 7.708 ± 0.105	0.328ArgCys: 0.328 ± 0.016	4.023ArgAsp: 4.023 ± 0.071	4.285ArgGlu: 4.285 ± 0.063	2.409ArgPhe: 2.409 ± 0.051	5.097ArgGly: 5.097 ± 0.075	1.399ArgHis: 1.399 ± 0.037	3.766ArgIle: 3.766 ± 0.062	1.571ArgLys: 1.571 ± 0.042	7.209ArgLeu: 7.209 ± 0.102	1.634ArgMet: 1.634 ± 0.041	1.363ArgAsn: 1.363 ± 0.041	3.539ArgPro: 3.539 ± 0.06	1.988ArgGln: 1.988 ± 0.046	5.908ArgArg: 5.908 ± 0.102	4.295ArgSer: 4.295 ± 0.077	4.55ArgThr: 4.55 ± 0.071	4.848ArgVal: 4.848 ± 0.067	0.988ArgTrp: 0.988 ± 0.033	1.355ArgTyr: 1.355 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	8.086SerAla: 8.086 ± 0.101	0.302SerCys: 0.302 ± 0.019	3.552SerAsp: 3.552 ± 0.064	3.355SerGlu: 3.355 ± 0.062	1.884SerPhe: 1.884 ± 0.043	6.351SerGly: 6.351 ± 0.099	1.12SerHis: 1.12 ± 0.036	2.58SerIle: 2.58 ± 0.058	1.312SerLys: 1.312 ± 0.04	5.637SerLeu: 5.637 ± 0.076	1.228SerMet: 1.228 ± 0.034	1.186SerAsn: 1.186 ± 0.035	3.43SerPro: 3.43 ± 0.061	1.652SerGln: 1.652 ± 0.046	4.187SerArg: 4.187 ± 0.065	3.73SerSer: 3.73 ± 0.076	4.024SerThr: 4.024 ± 0.064	4.697SerVal: 4.697 ± 0.067	0.832SerTrp: 0.832 ± 0.028	1.171SerTyr: 1.171 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	8.189ThrAla: 8.189 ± 0.108	0.303ThrCys: 0.303 ± 0.018	4.069ThrAsp: 4.069 ± 0.062	3.823ThrGlu: 3.823 ± 0.061	1.897ThrPhe: 1.897 ± 0.047	6.367ThrGly: 6.367 ± 0.084	1.102ThrHis: 1.102 ± 0.034	3.189ThrIle: 3.189 ± 0.051	1.477ThrLys: 1.477 ± 0.039	5.549ThrLeu: 5.549 ± 0.079	1.256ThrMet: 1.256 ± 0.033	1.17ThrAsn: 1.17 ± 0.032	3.958ThrPro: 3.958 ± 0.067	1.516ThrGln: 1.516 ± 0.039	3.673ThrArg: 3.673 ± 0.061	3.742ThrSer: 3.742 ± 0.056	4.24ThrThr: 4.24 ± 0.074	5.846ThrVal: 5.846 ± 0.074	0.881ThrTrp: 0.881 ± 0.033	1.148ThrTyr: 1.148 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	10.103ValAla: 10.103 ± 0.099	0.619ValCys: 0.619 ± 0.025	5.999ValAsp: 5.999 ± 0.083	4.841ValGlu: 4.841 ± 0.077	2.925ValPhe: 2.925 ± 0.054	6.614ValGly: 6.614 ± 0.08	1.619ValHis: 1.619 ± 0.034	4.537ValIle: 4.537 ± 0.08	1.654ValLys: 1.654 ± 0.041	9.007ValLeu: 9.007 ± 0.119	1.495ValMet: 1.495 ± 0.042	1.638ValAsn: 1.638 ± 0.043	4.664ValPro: 4.664 ± 0.058	1.661ValGln: 1.661 ± 0.041	5.452ValArg: 5.452 ± 0.081	5.101ValSer: 5.101 ± 0.065	5.688ValThr: 5.688 ± 0.076	7.861ValVal: 7.861 ± 0.099	0.979ValTrp: 0.979 ± 0.03	1.341ValTyr: 1.341 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.428TrpAla: 1.428 ± 0.036	0.095TrpCys: 0.095 ± 0.01	0.721TrpAsp: 0.721 ± 0.027	0.685TrpGlu: 0.685 ± 0.026	0.553TrpPhe: 0.553 ± 0.023	0.94TrpGly: 0.94 ± 0.03	0.274TrpHis: 0.274 ± 0.016	0.721TrpIle: 0.721 ± 0.026	0.381TrpLys: 0.381 ± 0.02	1.519TrpLeu: 1.519 ± 0.04	0.353TrpMet: 0.353 ± 0.017	0.378TrpAsn: 0.378 ± 0.02	0.641TrpPro: 0.641 ± 0.026	0.493TrpGln: 0.493 ± 0.024	0.897TrpArg: 0.897 ± 0.032	0.828TrpSer: 0.828 ± 0.029	0.808TrpThr: 0.808 ± 0.032	1.134TrpVal: 1.134 ± 0.034	0.303TrpTrp: 0.303 ± 0.019	0.248TrpTyr: 0.248 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.094TyrAla: 2.094 ± 0.043	0.12TyrCys: 0.12 ± 0.01	1.232TyrAsp: 1.232 ± 0.032	0.983TyrGlu: 0.983 ± 0.032	0.781TyrPhe: 0.781 ± 0.031	1.893TyrGly: 1.893 ± 0.059	0.343TyrHis: 0.343 ± 0.017	0.727TyrIle: 0.727 ± 0.027	0.362TyrLys: 0.362 ± 0.021	2.132TyrLeu: 2.132 ± 0.046	0.284TyrMet: 0.284 ± 0.016	0.384TyrAsn: 0.384 ± 0.019	1.014TyrPro: 1.014 ± 0.034	0.536TyrGln: 0.536 ± 0.022	1.531TyrArg: 1.531 ± 0.038	1.207TyrSer: 1.207 ± 0.033	1.054TyrThr: 1.054 ± 0.031	1.468TyrVal: 1.468 ± 0.039	0.288TyrTrp: 0.288 ± 0.016	0.443TyrTyr: 0.443 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3216 proteins (1086725 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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