Amino acid dipepetide frequency for Halobacillus sp. BBL2006

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.441AlaAla: 5.441 ± 0.093
0.561AlaCys: 0.561 ± 0.025
3.435AlaAsp: 3.435 ± 0.059
4.744AlaGlu: 4.744 ± 0.068
3.363AlaPhe: 3.363 ± 0.061
5.241AlaGly: 5.241 ± 0.078
1.362AlaHis: 1.362 ± 0.038
5.374AlaIle: 5.374 ± 0.08
4.236AlaLys: 4.236 ± 0.063
7.0AlaLeu: 7.0 ± 0.098
2.039AlaMet: 2.039 ± 0.046
2.526AlaAsn: 2.526 ± 0.051
2.176AlaPro: 2.176 ± 0.051
2.225AlaGln: 2.225 ± 0.047
2.687AlaArg: 2.687 ± 0.05
4.347AlaSer: 4.347 ± 0.066
3.347AlaThr: 3.347 ± 0.055
5.363AlaVal: 5.363 ± 0.079
0.672AlaTrp: 0.672 ± 0.027
2.345AlaTyr: 2.345 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.377CysAla: 0.377 ± 0.019
0.076CysCys: 0.076 ± 0.009
0.381CysAsp: 0.381 ± 0.02
0.439CysGlu: 0.439 ± 0.022
0.255CysPhe: 0.255 ± 0.016
0.612CysGly: 0.612 ± 0.025
0.186CysHis: 0.186 ± 0.012
0.384CysIle: 0.384 ± 0.018
0.328CysLys: 0.328 ± 0.018
0.578CysLeu: 0.578 ± 0.024
0.167CysMet: 0.167 ± 0.014
0.252CysAsn: 0.252 ± 0.016
0.342CysPro: 0.342 ± 0.019
0.223CysGln: 0.223 ± 0.015
0.269CysArg: 0.269 ± 0.017
0.397CysSer: 0.397 ± 0.021
0.334CysThr: 0.334 ± 0.018
0.351CysVal: 0.351 ± 0.018
0.06CysTrp: 0.06 ± 0.007
0.225CysTyr: 0.225 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.328AspAla: 3.328 ± 0.063
0.339AspCys: 0.339 ± 0.02
2.792AspAsp: 2.792 ± 0.059
4.876AspGlu: 4.876 ± 0.082
2.513AspPhe: 2.513 ± 0.049
3.677AspGly: 3.677 ± 0.059
1.632AspHis: 1.632 ± 0.036
3.732AspIle: 3.732 ± 0.063
2.988AspLys: 2.988 ± 0.064
5.348AspLeu: 5.348 ± 0.087
1.446AspMet: 1.446 ± 0.04
1.73AspAsn: 1.73 ± 0.042
2.314AspPro: 2.314 ± 0.046
2.805AspGln: 2.805 ± 0.057
2.668AspArg: 2.668 ± 0.052
2.798AspSer: 2.798 ± 0.053
2.491AspThr: 2.491 ± 0.05
4.098AspVal: 4.098 ± 0.067
0.726AspTrp: 0.726 ± 0.024
2.344AspTyr: 2.344 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
5.859GluAla: 5.859 ± 0.079
0.355GluCys: 0.355 ± 0.021
4.673GluAsp: 4.673 ± 0.08
8.198GluGlu: 8.198 ± 0.107
2.542GluPhe: 2.542 ± 0.055
5.071GluGly: 5.071 ± 0.074
1.583GluHis: 1.583 ± 0.034
5.166GluIle: 5.166 ± 0.075
6.565GluLys: 6.565 ± 0.084
7.033GluLeu: 7.033 ± 0.084
2.429GluMet: 2.429 ± 0.05
3.606GluAsn: 3.606 ± 0.061
2.117GluPro: 2.117 ± 0.046
3.593GluGln: 3.593 ± 0.068
3.828GluArg: 3.828 ± 0.066
3.918GluSer: 3.918 ± 0.063
4.012GluThr: 4.012 ± 0.065
5.704GluVal: 5.704 ± 0.076
1.014GluTrp: 1.014 ± 0.033
2.279GluTyr: 2.279 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
2.901PheAla: 2.901 ± 0.054
0.307PheCys: 0.307 ± 0.017
2.484PheAsp: 2.484 ± 0.047
2.978PheGlu: 2.978 ± 0.057
2.415PhePhe: 2.415 ± 0.058
3.344PheGly: 3.344 ± 0.07
1.125PheHis: 1.125 ± 0.033
3.638PheIle: 3.638 ± 0.068
2.337PheLys: 2.337 ± 0.05
4.484PheLeu: 4.484 ± 0.091
1.235PheMet: 1.235 ± 0.037
1.745PheAsn: 1.745 ± 0.04
1.705PhePro: 1.705 ± 0.047
1.787PheGln: 1.787 ± 0.043
1.626PheArg: 1.626 ± 0.041
3.201PheSer: 3.201 ± 0.06
2.617PheThr: 2.617 ± 0.049
3.096PheVal: 3.096 ± 0.056
0.487PheTrp: 0.487 ± 0.022
1.755PheTyr: 1.755 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
5.06GlyAla: 5.06 ± 0.085
0.563GlyCys: 0.563 ± 0.028
3.527GlyAsp: 3.527 ± 0.063
4.996GlyGlu: 4.996 ± 0.072
3.397GlyPhe: 3.397 ± 0.064
5.111GlyGly: 5.111 ± 0.094
1.429GlyHis: 1.429 ± 0.04
5.508GlyIle: 5.508 ± 0.073
4.632GlyLys: 4.632 ± 0.075
6.661GlyLeu: 6.661 ± 0.09
2.332GlyMet: 2.332 ± 0.05
2.535GlyAsn: 2.535 ± 0.052
1.893GlyPro: 1.893 ± 0.045
2.255GlyGln: 2.255 ± 0.044
2.82GlyArg: 2.82 ± 0.056
4.122GlySer: 4.122 ± 0.059
3.951GlyThr: 3.951 ± 0.064
5.437GlyVal: 5.437 ± 0.071
0.849GlyTrp: 0.849 ± 0.027
2.926GlyTyr: 2.926 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.359HisAla: 1.359 ± 0.037
0.191HisCys: 0.191 ± 0.013
1.091HisAsp: 1.091 ± 0.034
1.618HisGlu: 1.618 ± 0.04
1.095HisPhe: 1.095 ± 0.032
1.474HisGly: 1.474 ± 0.04
0.755HisHis: 0.755 ± 0.028
1.466HisIle: 1.466 ± 0.037
1.166HisLys: 1.166 ± 0.033
2.324HisLeu: 2.324 ± 0.052
0.619HisMet: 0.619 ± 0.025
0.828HisAsn: 0.828 ± 0.026
1.281HisPro: 1.281 ± 0.039
1.057HisGln: 1.057 ± 0.031
0.944HisArg: 0.944 ± 0.027
1.373HisSer: 1.373 ± 0.042
1.173HisThr: 1.173 ± 0.031
1.523HisVal: 1.523 ± 0.038
0.241HisTrp: 0.241 ± 0.016
0.928HisTyr: 0.928 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.532IleAla: 5.532 ± 0.081
0.511IleCys: 0.511 ± 0.022
4.291IleAsp: 4.291 ± 0.068
5.501IleGlu: 5.501 ± 0.077
3.138IlePhe: 3.138 ± 0.064
5.727IleGly: 5.727 ± 0.081
1.774IleHis: 1.774 ± 0.041
5.131IleIle: 5.131 ± 0.081
3.858IleLys: 3.858 ± 0.066
6.455IleLeu: 6.455 ± 0.091
1.712IleMet: 1.712 ± 0.044
2.873IleAsn: 2.873 ± 0.052
3.189IlePro: 3.189 ± 0.061
2.907IleGln: 2.907 ± 0.045
2.984IleArg: 2.984 ± 0.052
4.573IleSer: 4.573 ± 0.06
3.847IleThr: 3.847 ± 0.06
5.118IleVal: 5.118 ± 0.075
0.602IleTrp: 0.602 ± 0.024
2.233IleTyr: 2.233 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
4.527LysAla: 4.527 ± 0.071
0.236LysCys: 0.236 ± 0.015
4.003LysAsp: 4.003 ± 0.063
6.854LysGlu: 6.854 ± 0.097
1.84LysPhe: 1.84 ± 0.043
4.412LysGly: 4.412 ± 0.068
1.335LysHis: 1.335 ± 0.037
3.782LysIle: 3.782 ± 0.063
5.305LysLys: 5.305 ± 0.074
5.13LysLeu: 5.13 ± 0.075
1.951LysMet: 1.951 ± 0.042
3.041LysAsn: 3.041 ± 0.058
2.201LysPro: 2.201 ± 0.046
3.272LysGln: 3.272 ± 0.06
3.346LysArg: 3.346 ± 0.063
3.483LysSer: 3.483 ± 0.054
3.207LysThr: 3.207 ± 0.058
4.557LysVal: 4.557 ± 0.063
0.817LysTrp: 0.817 ± 0.027
1.856LysTyr: 1.856 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
6.874LeuAla: 6.874 ± 0.094
0.54LeuCys: 0.54 ± 0.023
5.04LeuAsp: 5.04 ± 0.074
6.823LeuGlu: 6.823 ± 0.092
4.801LeuPhe: 4.801 ± 0.09
6.48LeuGly: 6.48 ± 0.081
2.031LeuHis: 2.031 ± 0.047
6.771LeuIle: 6.771 ± 0.098
6.18LeuLys: 6.18 ± 0.081
9.488LeuLeu: 9.488 ± 0.13
2.697LeuMet: 2.697 ± 0.047
4.205LeuAsn: 4.205 ± 0.067
3.946LeuPro: 3.946 ± 0.064
3.425LeuGln: 3.425 ± 0.061
3.668LeuArg: 3.668 ± 0.056
6.819LeuSer: 6.819 ± 0.077
5.409LeuThr: 5.409 ± 0.077
6.174LeuVal: 6.174 ± 0.08
0.899LeuTrp: 0.899 ± 0.029
3.023LeuTyr: 3.023 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
2.092MetAla: 2.092 ± 0.045
0.13MetCys: 0.13 ± 0.01
1.784MetAsp: 1.784 ± 0.044
2.172MetGlu: 2.172 ± 0.045
1.065MetPhe: 1.065 ± 0.034
1.832MetGly: 1.832 ± 0.048
0.425MetHis: 0.425 ± 0.018
2.265MetIle: 2.265 ± 0.051
2.596MetLys: 2.596 ± 0.051
2.44MetLeu: 2.44 ± 0.051
0.947MetMet: 0.947 ± 0.031
1.724MetAsn: 1.724 ± 0.042
1.03MetPro: 1.03 ± 0.038
0.939MetGln: 0.939 ± 0.031
1.084MetArg: 1.084 ± 0.03
1.873MetSer: 1.873 ± 0.038
1.848MetThr: 1.848 ± 0.041
2.026MetVal: 2.026 ± 0.049
0.194MetTrp: 0.194 ± 0.015
0.752MetTyr: 0.752 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.525AsnAla: 2.525 ± 0.053
0.272AsnCys: 0.272 ± 0.017
2.337AsnAsp: 2.337 ± 0.052
3.495AsnGlu: 3.495 ± 0.056
1.588AsnPhe: 1.588 ± 0.035
2.972AsnGly: 2.972 ± 0.06
1.124AsnHis: 1.124 ± 0.033
2.866AsnIle: 2.866 ± 0.058
2.562AsnLys: 2.562 ± 0.055
3.499AsnLeu: 3.499 ± 0.057
1.1AsnMet: 1.1 ± 0.032
1.704AsnAsn: 1.704 ± 0.04
2.038AsnPro: 2.038 ± 0.041
2.123AsnGln: 2.123 ± 0.051
2.046AsnArg: 2.046 ± 0.046
2.084AsnSer: 2.084 ± 0.047
1.952AsnThr: 1.952 ± 0.041
2.912AsnVal: 2.912 ± 0.056
0.51AsnTrp: 0.51 ± 0.022
1.495AsnTyr: 1.495 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
2.351ProAla: 2.351 ± 0.055
0.21ProCys: 0.21 ± 0.014
2.37ProAsp: 2.37 ± 0.047
3.483ProGlu: 3.483 ± 0.057
2.017ProPhe: 2.017 ± 0.05
2.47ProGly: 2.47 ± 0.059
0.873ProHis: 0.873 ± 0.032
2.63ProIle: 2.63 ± 0.052
2.171ProLys: 2.171 ± 0.038
3.598ProLeu: 3.598 ± 0.062
1.033ProMet: 1.033 ± 0.033
1.56ProAsn: 1.56 ± 0.039
1.082ProPro: 1.082 ± 0.032
1.129ProGln: 1.129 ± 0.041
1.161ProArg: 1.161 ± 0.035
2.51ProSer: 2.51 ± 0.048
1.915ProThr: 1.915 ± 0.04
3.021ProVal: 3.021 ± 0.05
0.428ProTrp: 0.428 ± 0.017
1.529ProTyr: 1.529 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.925GlnAla: 2.925 ± 0.051
0.177GlnCys: 0.177 ± 0.014
1.852GlnAsp: 1.852 ± 0.048
3.232GlnGlu: 3.232 ± 0.059
1.64GlnPhe: 1.64 ± 0.035
2.431GlnGly: 2.431 ± 0.05
0.813GlnHis: 0.813 ± 0.027
2.447GlnIle: 2.447 ± 0.046
2.8GlnLys: 2.8 ± 0.052
4.087GlnLeu: 4.087 ± 0.073
1.398GlnMet: 1.398 ± 0.041
1.605GlnAsn: 1.605 ± 0.041
1.451GlnPro: 1.451 ± 0.041
1.859GlnGln: 1.859 ± 0.048
1.624GlnArg: 1.624 ± 0.039
2.506GlnSer: 2.506 ± 0.049
2.113GlnThr: 2.113 ± 0.043
2.65GlnVal: 2.65 ± 0.053
0.485GlnTrp: 0.485 ± 0.024
1.398GlnTyr: 1.398 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.423ArgAla: 2.423 ± 0.047
0.207ArgCys: 0.207 ± 0.015
2.132ArgAsp: 2.132 ± 0.042
3.441ArgGlu: 3.441 ± 0.062
2.006ArgPhe: 2.006 ± 0.046
2.46ArgGly: 2.46 ± 0.054
0.872ArgHis: 0.872 ± 0.029
2.927ArgIle: 2.927 ± 0.053
3.243ArgLys: 3.243 ± 0.059
4.216ArgLeu: 4.216 ± 0.075
1.417ArgMet: 1.417 ± 0.039
1.848ArgAsn: 1.848 ± 0.044
1.453ArgPro: 1.453 ± 0.036
1.632ArgGln: 1.632 ± 0.038
2.025ArgArg: 2.025 ± 0.054
2.47ArgSer: 2.47 ± 0.05
2.246ArgThr: 2.246 ± 0.038
2.878ArgVal: 2.878 ± 0.053
0.442ArgTrp: 0.442 ± 0.019
1.61ArgTyr: 1.61 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.6SerAla: 3.6 ± 0.059
0.351SerCys: 0.351 ± 0.016
3.128SerAsp: 3.128 ± 0.049
4.403SerGlu: 4.403 ± 0.063
3.307SerPhe: 3.307 ± 0.07
4.518SerGly: 4.518 ± 0.065
1.357SerHis: 1.357 ± 0.039
4.82SerIle: 4.82 ± 0.064
3.712SerLys: 3.712 ± 0.061
6.298SerLeu: 6.298 ± 0.076
1.905SerMet: 1.905 ± 0.041
2.464SerAsn: 2.464 ± 0.051
2.292SerPro: 2.292 ± 0.047
2.165SerGln: 2.165 ± 0.048
2.443SerArg: 2.443 ± 0.049
4.32SerSer: 4.32 ± 0.074
3.076SerThr: 3.076 ± 0.053
4.125SerVal: 4.125 ± 0.058
0.667SerTrp: 0.667 ± 0.027
2.38SerTyr: 2.38 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
3.688ThrAla: 3.688 ± 0.063
0.309ThrCys: 0.309 ± 0.02
2.688ThrAsp: 2.688 ± 0.053
3.496ThrGlu: 3.496 ± 0.052
2.682ThrPhe: 2.682 ± 0.047
4.123ThrGly: 4.123 ± 0.058
1.127ThrHis: 1.127 ± 0.03
4.253ThrIle: 4.253 ± 0.069
3.111ThrLys: 3.111 ± 0.061
5.174ThrLeu: 5.174 ± 0.067
1.457ThrMet: 1.457 ± 0.041
2.158ThrAsn: 2.158 ± 0.045
2.375ThrPro: 2.375 ± 0.055
1.513ThrGln: 1.513 ± 0.039
1.928ThrArg: 1.928 ± 0.042
3.277ThrSer: 3.277 ± 0.059
2.799ThrThr: 2.799 ± 0.056
4.034ThrVal: 4.034 ± 0.07
0.566ThrTrp: 0.566 ± 0.02
2.012ThrTyr: 2.012 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
4.896ValAla: 4.896 ± 0.071
0.578ValCys: 0.578 ± 0.026
4.074ValAsp: 4.074 ± 0.071
5.303ValGlu: 5.303 ± 0.082
3.173ValPhe: 3.173 ± 0.065
4.767ValGly: 4.767 ± 0.071
1.594ValHis: 1.594 ± 0.039
5.561ValIle: 5.561 ± 0.072
4.519ValLys: 4.519 ± 0.071
6.828ValLeu: 6.828 ± 0.083
2.023ValMet: 2.023 ± 0.046
2.903ValAsn: 2.903 ± 0.058
2.827ValPro: 2.827 ± 0.046
2.492ValGln: 2.492 ± 0.053
2.737ValArg: 2.737 ± 0.054
4.567ValSer: 4.567 ± 0.065
4.077ValThr: 4.077 ± 0.065
5.203ValVal: 5.203 ± 0.078
0.687ValTrp: 0.687 ± 0.026
2.404ValTyr: 2.404 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.631TrpAla: 0.631 ± 0.026
0.063TrpCys: 0.063 ± 0.007
0.525TrpAsp: 0.525 ± 0.023
0.707TrpGlu: 0.707 ± 0.03
0.577TrpPhe: 0.577 ± 0.023
0.718TrpGly: 0.718 ± 0.029
0.19TrpHis: 0.19 ± 0.013
0.882TrpIle: 0.882 ± 0.03
0.82TrpLys: 0.82 ± 0.028
1.222TrpLeu: 1.222 ± 0.042
0.416TrpMet: 0.416 ± 0.02
0.553TrpAsn: 0.553 ± 0.022
0.307TrpPro: 0.307 ± 0.016
0.389TrpGln: 0.389 ± 0.019
0.414TrpArg: 0.414 ± 0.02
0.664TrpSer: 0.664 ± 0.026
0.557TrpThr: 0.557 ± 0.025
0.769TrpVal: 0.769 ± 0.027
0.154TrpTrp: 0.154 ± 0.013
0.363TrpTyr: 0.363 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.098TyrAla: 2.098 ± 0.049
0.263TyrCys: 0.263 ± 0.017
2.037TyrAsp: 2.037 ± 0.047
2.787TyrGlu: 2.787 ± 0.053
1.854TyrPhe: 1.854 ± 0.043
2.562TyrGly: 2.562 ± 0.054
0.921TyrHis: 0.921 ± 0.031
2.353TyrIle: 2.353 ± 0.048
2.027TyrLys: 2.027 ± 0.046
3.427TyrLeu: 3.427 ± 0.059
0.893TyrMet: 0.893 ± 0.029
1.344TyrAsn: 1.344 ± 0.037
1.521TyrPro: 1.521 ± 0.033
1.704TyrGln: 1.704 ± 0.045
1.646TyrArg: 1.646 ± 0.038
2.027TyrSer: 2.027 ± 0.05
1.796TyrThr: 1.796 ± 0.039
2.176TyrVal: 2.176 ± 0.048
0.42TyrTrp: 0.42 ± 0.021
1.414TyrTyr: 1.414 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4119 proteins (1069533 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski