Amino acid dipepetide frequency for Staphylococcus phage phi2958PVL

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.702AlaAla: 2.702 ± 0.806
0.213AlaCys: 0.213 ± 0.133
2.844AlaAsp: 2.844 ± 0.489
4.195AlaGlu: 4.195 ± 0.501
1.635AlaPhe: 1.635 ± 0.278
3.626AlaGly: 3.626 ± 0.825
0.995AlaHis: 0.995 ± 0.221
4.408AlaIle: 4.408 ± 0.671
5.973AlaLys: 5.973 ± 1.048
5.475AlaLeu: 5.475 ± 0.692
1.493AlaMet: 1.493 ± 0.292
4.124AlaAsn: 4.124 ± 0.853
1.422AlaPro: 1.422 ± 0.378
1.493AlaGln: 1.493 ± 0.396
2.275AlaArg: 2.275 ± 0.346
4.764AlaSer: 4.764 ± 0.792
3.413AlaThr: 3.413 ± 0.509
2.489AlaVal: 2.489 ± 0.546
1.138AlaTrp: 1.138 ± 0.388
2.844AlaTyr: 2.844 ± 0.508
0.0AlaXaa: 0.0 ± 0.0
Cys
0.213CysAla: 0.213 ± 0.121
0.0CysCys: 0.0 ± 0.0
0.142CysAsp: 0.142 ± 0.105
0.213CysGlu: 0.213 ± 0.182
0.427CysPhe: 0.427 ± 0.192
0.142CysGly: 0.142 ± 0.103
0.142CysHis: 0.142 ± 0.109
0.427CysIle: 0.427 ± 0.175
0.498CysLys: 0.498 ± 0.195
0.64CysLeu: 0.64 ± 0.217
0.071CysMet: 0.071 ± 0.081
0.213CysAsn: 0.213 ± 0.119
0.071CysPro: 0.071 ± 0.068
0.142CysGln: 0.142 ± 0.097
0.356CysArg: 0.356 ± 0.201
0.213CysSer: 0.213 ± 0.123
0.284CysThr: 0.284 ± 0.138
0.071CysVal: 0.071 ± 0.08
0.0CysTrp: 0.0 ± 0.0
0.213CysTyr: 0.213 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
3.057AspAla: 3.057 ± 0.569
0.142AspCys: 0.142 ± 0.113
3.911AspAsp: 3.911 ± 0.645
4.622AspGlu: 4.622 ± 0.762
3.413AspPhe: 3.413 ± 0.481
3.555AspGly: 3.555 ± 0.651
0.782AspHis: 0.782 ± 0.187
5.191AspIle: 5.191 ± 0.469
6.684AspLys: 6.684 ± 0.706
5.83AspLeu: 5.83 ± 0.651
1.92AspMet: 1.92 ± 0.311
2.986AspAsn: 2.986 ± 0.444
1.209AspPro: 1.209 ± 0.362
1.493AspGln: 1.493 ± 0.261
1.849AspArg: 1.849 ± 0.376
3.697AspSer: 3.697 ± 0.629
4.124AspThr: 4.124 ± 0.567
3.911AspVal: 3.911 ± 0.438
0.782AspTrp: 0.782 ± 0.24
3.129AspTyr: 3.129 ± 0.545
0.0AspXaa: 0.0 ± 0.0
Glu
4.266GluAla: 4.266 ± 0.51
0.356GluCys: 0.356 ± 0.209
4.622GluAsp: 4.622 ± 0.675
7.466GluGlu: 7.466 ± 1.244
3.2GluPhe: 3.2 ± 0.593
3.626GluGly: 3.626 ± 0.519
0.995GluHis: 0.995 ± 0.276
5.617GluIle: 5.617 ± 0.937
8.106GluLys: 8.106 ± 0.983
6.968GluLeu: 6.968 ± 0.786
2.489GluMet: 2.489 ± 0.471
4.906GluAsn: 4.906 ± 0.586
1.209GluPro: 1.209 ± 0.377
3.2GluGln: 3.2 ± 0.549
2.844GluArg: 2.844 ± 0.589
3.2GluSer: 3.2 ± 0.451
4.053GluThr: 4.053 ± 0.657
3.911GluVal: 3.911 ± 0.495
0.995GluTrp: 0.995 ± 0.211
2.773GluTyr: 2.773 ± 0.698
0.0GluXaa: 0.0 ± 0.0
Phe
1.849PheAla: 1.849 ± 0.409
0.356PheCys: 0.356 ± 0.165
3.129PheAsp: 3.129 ± 0.383
2.915PheGlu: 2.915 ± 0.485
0.995PhePhe: 0.995 ± 0.233
3.057PheGly: 3.057 ± 0.54
0.427PheHis: 0.427 ± 0.155
3.413PheIle: 3.413 ± 0.579
4.124PheLys: 4.124 ± 0.602
2.56PheLeu: 2.56 ± 0.434
0.924PheMet: 0.924 ± 0.216
3.555PheAsn: 3.555 ± 0.496
0.782PhePro: 0.782 ± 0.255
1.351PheGln: 1.351 ± 0.231
1.067PheArg: 1.067 ± 0.27
1.778PheSer: 1.778 ± 0.33
1.849PheThr: 1.849 ± 0.403
2.275PheVal: 2.275 ± 0.518
0.284PheTrp: 0.284 ± 0.136
2.062PheTyr: 2.062 ± 0.436
0.0PheXaa: 0.0 ± 0.0
Gly
3.911GlyAla: 3.911 ± 1.028
0.213GlyCys: 0.213 ± 0.125
4.266GlyAsp: 4.266 ± 0.519
3.413GlyGlu: 3.413 ± 0.458
2.56GlyPhe: 2.56 ± 0.42
5.262GlyGly: 5.262 ± 1.237
1.351GlyHis: 1.351 ± 0.398
3.555GlyIle: 3.555 ± 0.522
5.83GlyLys: 5.83 ± 0.673
5.688GlyLeu: 5.688 ± 0.935
1.138GlyMet: 1.138 ± 0.374
3.413GlyAsn: 3.413 ± 0.648
0.995GlyPro: 0.995 ± 0.225
1.92GlyGln: 1.92 ± 0.446
2.204GlyArg: 2.204 ± 0.456
3.982GlySer: 3.982 ± 0.593
3.768GlyThr: 3.768 ± 0.542
4.408GlyVal: 4.408 ± 0.684
0.995GlyTrp: 0.995 ± 0.325
2.56GlyTyr: 2.56 ± 0.48
0.0GlyXaa: 0.0 ± 0.0
His
1.067HisAla: 1.067 ± 0.286
0.071HisCys: 0.071 ± 0.068
0.64HisAsp: 0.64 ± 0.254
1.209HisGlu: 1.209 ± 0.282
0.711HisPhe: 0.711 ± 0.197
1.138HisGly: 1.138 ± 0.336
0.427HisHis: 0.427 ± 0.207
1.706HisIle: 1.706 ± 0.48
1.138HisLys: 1.138 ± 0.248
1.493HisLeu: 1.493 ± 0.313
0.284HisMet: 0.284 ± 0.164
1.209HisAsn: 1.209 ± 0.316
0.853HisPro: 0.853 ± 0.219
0.569HisGln: 0.569 ± 0.189
0.711HisArg: 0.711 ± 0.199
1.138HisSer: 1.138 ± 0.233
1.28HisThr: 1.28 ± 0.309
0.924HisVal: 0.924 ± 0.335
0.213HisTrp: 0.213 ± 0.14
1.067HisTyr: 1.067 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
4.408IleAla: 4.408 ± 0.644
0.427IleCys: 0.427 ± 0.218
4.906IleAsp: 4.906 ± 0.685
5.83IleGlu: 5.83 ± 0.628
2.133IlePhe: 2.133 ± 0.5
3.271IleGly: 3.271 ± 0.486
1.706IleHis: 1.706 ± 0.317
4.266IleIle: 4.266 ± 0.734
7.679IleLys: 7.679 ± 0.832
4.977IleLeu: 4.977 ± 0.725
1.849IleMet: 1.849 ± 0.373
4.764IleAsn: 4.764 ± 0.504
2.56IlePro: 2.56 ± 0.434
1.493IleGln: 1.493 ± 0.276
3.484IleArg: 3.484 ± 0.496
5.191IleSer: 5.191 ± 0.588
4.124IleThr: 4.124 ± 0.53
4.053IleVal: 4.053 ± 0.587
0.498IleTrp: 0.498 ± 0.219
2.915IleTyr: 2.915 ± 0.53
0.071IleXaa: 0.071 ± 0.059
Lys
7.964LysAla: 7.964 ± 1.055
0.142LysCys: 0.142 ± 0.087
5.475LysAsp: 5.475 ± 0.564
7.821LysGlu: 7.821 ± 1.002
1.778LysPhe: 1.778 ± 0.403
5.119LysGly: 5.119 ± 0.936
1.92LysHis: 1.92 ± 0.427
6.755LysIle: 6.755 ± 0.884
7.75LysLys: 7.75 ± 0.857
9.101LysLeu: 9.101 ± 1.047
2.915LysMet: 2.915 ± 0.41
5.973LysAsn: 5.973 ± 0.744
2.346LysPro: 2.346 ± 0.475
4.906LysGln: 4.906 ± 0.6
3.555LysArg: 3.555 ± 0.693
6.115LysSer: 6.115 ± 1.172
5.333LysThr: 5.333 ± 0.583
4.977LysVal: 4.977 ± 0.667
1.991LysTrp: 1.991 ± 0.463
5.048LysTyr: 5.048 ± 0.623
0.0LysXaa: 0.0 ± 0.0
Leu
4.195LeuAla: 4.195 ± 0.783
0.498LeuCys: 0.498 ± 0.222
5.475LeuAsp: 5.475 ± 0.999
6.826LeuGlu: 6.826 ± 0.709
2.986LeuPhe: 2.986 ± 0.401
4.906LeuGly: 4.906 ± 0.874
1.209LeuHis: 1.209 ± 0.37
5.475LeuIle: 5.475 ± 0.639
8.746LeuLys: 8.746 ± 1.297
6.399LeuLeu: 6.399 ± 0.848
2.062LeuMet: 2.062 ± 0.376
6.115LeuAsn: 6.115 ± 0.618
3.413LeuPro: 3.413 ± 0.599
2.986LeuGln: 2.986 ± 0.544
3.2LeuArg: 3.2 ± 0.519
5.475LeuSer: 5.475 ± 0.701
5.617LeuThr: 5.617 ± 0.736
3.911LeuVal: 3.911 ± 0.467
0.498LeuTrp: 0.498 ± 0.188
3.342LeuTyr: 3.342 ± 0.816
0.0LeuXaa: 0.0 ± 0.0
Met
0.995MetAla: 0.995 ± 0.239
0.142MetCys: 0.142 ± 0.097
1.422MetAsp: 1.422 ± 0.379
1.138MetGlu: 1.138 ± 0.331
0.924MetPhe: 0.924 ± 0.327
1.493MetGly: 1.493 ± 0.521
0.569MetHis: 0.569 ± 0.24
1.422MetIle: 1.422 ± 0.281
3.129MetLys: 3.129 ± 0.557
1.991MetLeu: 1.991 ± 0.489
0.427MetMet: 0.427 ± 0.232
1.92MetAsn: 1.92 ± 0.454
1.067MetPro: 1.067 ± 0.277
1.778MetGln: 1.778 ± 0.414
0.995MetArg: 0.995 ± 0.23
1.991MetSer: 1.991 ± 0.421
2.204MetThr: 2.204 ± 0.403
1.138MetVal: 1.138 ± 0.26
0.284MetTrp: 0.284 ± 0.152
0.995MetTyr: 0.995 ± 0.277
0.0MetXaa: 0.0 ± 0.0
Asn
3.697AsnAla: 3.697 ± 0.585
0.213AsnCys: 0.213 ± 0.118
4.124AsnAsp: 4.124 ± 0.464
4.551AsnGlu: 4.551 ± 0.671
2.275AsnPhe: 2.275 ± 0.457
4.622AsnGly: 4.622 ± 0.637
1.138AsnHis: 1.138 ± 0.315
4.337AsnIle: 4.337 ± 0.518
6.328AsnLys: 6.328 ± 0.721
5.262AsnLeu: 5.262 ± 0.649
1.138AsnMet: 1.138 ± 0.263
3.911AsnAsn: 3.911 ± 0.638
2.489AsnPro: 2.489 ± 0.325
2.915AsnGln: 2.915 ± 0.45
2.631AsnArg: 2.631 ± 0.42
4.408AsnSer: 4.408 ± 0.657
4.337AsnThr: 4.337 ± 0.447
3.129AsnVal: 3.129 ± 0.582
1.067AsnTrp: 1.067 ± 0.315
2.986AsnTyr: 2.986 ± 0.556
0.0AsnXaa: 0.0 ± 0.0
Pro
1.138ProAla: 1.138 ± 0.282
0.213ProCys: 0.213 ± 0.117
1.351ProAsp: 1.351 ± 0.302
2.204ProGlu: 2.204 ± 0.479
1.493ProPhe: 1.493 ± 0.375
1.706ProGly: 1.706 ± 0.334
0.213ProHis: 0.213 ± 0.105
1.493ProIle: 1.493 ± 0.331
2.275ProLys: 2.275 ± 0.459
2.489ProLeu: 2.489 ± 0.45
0.711ProMet: 0.711 ± 0.233
2.062ProAsn: 2.062 ± 0.358
0.64ProPro: 0.64 ± 0.243
1.28ProGln: 1.28 ± 0.306
1.209ProArg: 1.209 ± 0.231
2.489ProSer: 2.489 ± 0.371
1.706ProThr: 1.706 ± 0.429
1.493ProVal: 1.493 ± 0.435
0.213ProTrp: 0.213 ± 0.126
1.209ProTyr: 1.209 ± 0.335
0.0ProXaa: 0.0 ± 0.0
Gln
2.631GlnAla: 2.631 ± 0.416
0.213GlnCys: 0.213 ± 0.125
2.418GlnAsp: 2.418 ± 0.445
2.489GlnGlu: 2.489 ± 0.441
1.778GlnPhe: 1.778 ± 0.308
2.133GlnGly: 2.133 ± 0.463
0.569GlnHis: 0.569 ± 0.164
3.2GlnIle: 3.2 ± 0.518
3.057GlnLys: 3.057 ± 0.437
3.484GlnLeu: 3.484 ± 0.481
0.995GlnMet: 0.995 ± 0.299
2.275GlnAsn: 2.275 ± 0.49
1.067GlnPro: 1.067 ± 0.225
1.351GlnGln: 1.351 ± 0.412
1.92GlnArg: 1.92 ± 0.387
2.418GlnSer: 2.418 ± 0.391
1.28GlnThr: 1.28 ± 0.373
2.418GlnVal: 2.418 ± 0.445
0.427GlnTrp: 0.427 ± 0.174
1.493GlnTyr: 1.493 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
2.346ArgAla: 2.346 ± 0.493
0.0ArgCys: 0.0 ± 0.0
2.986ArgAsp: 2.986 ± 0.31
2.346ArgGlu: 2.346 ± 0.503
1.778ArgPhe: 1.778 ± 0.334
2.062ArgGly: 2.062 ± 0.345
0.711ArgHis: 0.711 ± 0.255
3.484ArgIle: 3.484 ± 0.465
4.124ArgLys: 4.124 ± 0.476
3.271ArgLeu: 3.271 ± 0.635
0.853ArgMet: 0.853 ± 0.246
2.773ArgAsn: 2.773 ± 0.341
0.569ArgPro: 0.569 ± 0.251
1.422ArgGln: 1.422 ± 0.366
1.564ArgArg: 1.564 ± 0.365
1.706ArgSer: 1.706 ± 0.332
2.56ArgThr: 2.56 ± 0.406
1.92ArgVal: 1.92 ± 0.37
0.356ArgTrp: 0.356 ± 0.156
2.062ArgTyr: 2.062 ± 0.56
0.0ArgXaa: 0.0 ± 0.0
Ser
3.626SerAla: 3.626 ± 0.828
0.284SerCys: 0.284 ± 0.151
4.764SerAsp: 4.764 ± 0.533
4.48SerGlu: 4.48 ± 0.681
2.631SerPhe: 2.631 ± 0.493
4.693SerGly: 4.693 ± 0.876
0.995SerHis: 0.995 ± 0.243
4.408SerIle: 4.408 ± 0.612
6.47SerLys: 6.47 ± 1.155
4.053SerLeu: 4.053 ± 0.496
2.275SerMet: 2.275 ± 0.38
4.551SerAsn: 4.551 ± 0.563
1.778SerPro: 1.778 ± 0.346
2.915SerGln: 2.915 ± 0.475
2.346SerArg: 2.346 ± 0.362
4.48SerSer: 4.48 ± 0.752
3.129SerThr: 3.129 ± 0.542
4.266SerVal: 4.266 ± 0.635
0.924SerTrp: 0.924 ± 0.232
2.204SerTyr: 2.204 ± 0.495
0.0SerXaa: 0.0 ± 0.0
Thr
3.413ThrAla: 3.413 ± 0.587
0.284ThrCys: 0.284 ± 0.145
2.986ThrAsp: 2.986 ± 0.47
4.124ThrGlu: 4.124 ± 0.612
2.702ThrPhe: 2.702 ± 0.343
4.124ThrGly: 4.124 ± 0.584
1.706ThrHis: 1.706 ± 0.365
4.693ThrIle: 4.693 ± 0.592
5.404ThrLys: 5.404 ± 0.695
4.337ThrLeu: 4.337 ± 0.674
0.995ThrMet: 0.995 ± 0.257
3.271ThrAsn: 3.271 ± 0.483
2.346ThrPro: 2.346 ± 0.379
1.564ThrGln: 1.564 ± 0.249
2.133ThrArg: 2.133 ± 0.358
3.484ThrSer: 3.484 ± 0.572
2.844ThrThr: 2.844 ± 0.523
4.622ThrVal: 4.622 ± 0.446
0.356ThrTrp: 0.356 ± 0.209
2.56ThrTyr: 2.56 ± 0.526
0.0ThrXaa: 0.0 ± 0.0
Val
3.057ValAla: 3.057 ± 0.558
0.356ValCys: 0.356 ± 0.209
3.84ValAsp: 3.84 ± 0.634
5.262ValGlu: 5.262 ± 0.62
2.275ValPhe: 2.275 ± 0.488
3.555ValGly: 3.555 ± 0.59
0.853ValHis: 0.853 ± 0.241
3.484ValIle: 3.484 ± 0.536
5.191ValLys: 5.191 ± 0.688
4.48ValLeu: 4.48 ± 0.581
1.422ValMet: 1.422 ± 0.315
3.697ValAsn: 3.697 ± 0.575
1.493ValPro: 1.493 ± 0.348
2.418ValGln: 2.418 ± 0.43
2.204ValArg: 2.204 ± 0.443
4.053ValSer: 4.053 ± 0.524
2.915ValThr: 2.915 ± 0.516
3.413ValVal: 3.413 ± 0.57
0.427ValTrp: 0.427 ± 0.196
2.275ValTyr: 2.275 ± 0.611
0.0ValXaa: 0.0 ± 0.0
Trp
0.569TrpAla: 0.569 ± 0.19
0.0TrpCys: 0.0 ± 0.0
0.427TrpAsp: 0.427 ± 0.237
0.995TrpGlu: 0.995 ± 0.318
1.209TrpPhe: 1.209 ± 0.329
0.569TrpGly: 0.569 ± 0.242
0.0TrpHis: 0.0 ± 0.0
0.853TrpIle: 0.853 ± 0.276
0.782TrpLys: 0.782 ± 0.262
1.067TrpLeu: 1.067 ± 0.253
0.427TrpMet: 0.427 ± 0.204
0.924TrpAsn: 0.924 ± 0.22
0.356TrpPro: 0.356 ± 0.262
0.498TrpGln: 0.498 ± 0.151
0.427TrpArg: 0.427 ± 0.158
1.209TrpSer: 1.209 ± 0.406
0.569TrpThr: 0.569 ± 0.187
0.711TrpVal: 0.711 ± 0.169
0.142TrpTrp: 0.142 ± 0.119
0.569TrpTyr: 0.569 ± 0.219
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.418TyrAla: 2.418 ± 0.299
0.356TyrCys: 0.356 ± 0.156
2.56TyrAsp: 2.56 ± 0.615
2.702TyrGlu: 2.702 ± 0.52
1.849TyrPhe: 1.849 ± 0.517
2.844TyrGly: 2.844 ± 0.608
1.138TyrHis: 1.138 ± 0.351
2.418TyrIle: 2.418 ± 0.449
3.697TyrLys: 3.697 ± 0.522
3.768TyrLeu: 3.768 ± 0.545
1.564TyrMet: 1.564 ± 0.339
2.915TyrAsn: 2.915 ± 0.661
0.924TyrPro: 0.924 ± 0.242
1.92TyrGln: 1.92 ± 0.371
1.92TyrArg: 1.92 ± 0.48
3.413TyrSer: 3.413 ± 0.444
2.56TyrThr: 2.56 ± 0.515
2.631TyrVal: 2.631 ± 0.453
0.711TyrTrp: 0.711 ± 0.199
1.706TyrTyr: 1.706 ± 0.455
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.071XaaSer: 0.071 ± 0.059
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (14065 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski