Apis mellifera associated microvirus 43

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5K2|A0A3Q8U5K2_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 43 OX=2494773 PE=3 SV=1
MM1 pKa = 7.86CKK3 pKa = 9.89RR4 pKa = 11.84AGWWPKK10 pKa = 9.96LVSRR14 pKa = 11.84TFTKK18 pKa = 10.13EE19 pKa = 3.25RR20 pKa = 11.84VMSEE24 pKa = 3.98RR25 pKa = 11.84PEE27 pKa = 4.14MNDD30 pKa = 3.62DD31 pKa = 3.23EE32 pKa = 5.93AIRR35 pKa = 11.84KK36 pKa = 9.81DD37 pKa = 3.72GEE39 pKa = 4.13DD40 pKa = 3.78EE41 pKa = 3.87ILVRR45 pKa = 11.84VPVQDD50 pKa = 3.64YY51 pKa = 11.11YY52 pKa = 11.5LDD54 pKa = 3.47QRR56 pKa = 11.84GRR58 pKa = 11.84EE59 pKa = 4.21MPDD62 pKa = 3.25PTPVAPPVNLQTSFNMTDD80 pKa = 2.8HH81 pKa = 6.94LRR83 pKa = 11.84RR84 pKa = 11.84LIRR87 pKa = 11.84SEE89 pKa = 3.74QLARR93 pKa = 11.84EE94 pKa = 4.46AYY96 pKa = 9.96AAGQEE101 pKa = 4.38TFEE104 pKa = 4.44EE105 pKa = 4.68ADD107 pKa = 4.06DD108 pKa = 4.48FDD110 pKa = 5.64VGDD113 pKa = 5.96DD114 pKa = 3.6PDD116 pKa = 4.63LYY118 pKa = 11.37NLSGYY123 pKa = 8.66EE124 pKa = 4.16NEE126 pKa = 4.71FEE128 pKa = 4.26PPAAQTLDD136 pKa = 3.34MSAKK140 pKa = 10.01QGAADD145 pKa = 4.86GSPEE149 pKa = 4.07GAKK152 pKa = 9.76QEE154 pKa = 4.15PLAAPPAEE162 pKa = 4.54PLSSAPQGAPQQ173 pKa = 3.54

Molecular weight: 19.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5H6|A0A3Q8U5H6_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 43 OX=2494773 PE=4 SV=1
MM1 pKa = 7.19ARR3 pKa = 11.84KK4 pKa = 9.8RR5 pKa = 11.84SRR7 pKa = 11.84SKK9 pKa = 11.17ARR11 pKa = 11.84DD12 pKa = 3.11HH13 pKa = 6.17STIATHH19 pKa = 5.48SVRR22 pKa = 11.84WNPIRR27 pKa = 11.84AASLVRR33 pKa = 11.84VSPVDD38 pKa = 3.49LRR40 pKa = 11.84IFEE43 pKa = 4.6DD44 pKa = 3.17RR45 pKa = 11.84RR46 pKa = 11.84TYY48 pKa = 10.7HH49 pKa = 6.7PAPAEE54 pKa = 3.98RR55 pKa = 11.84PALSFGGRR63 pKa = 11.84PVQLKK68 pKa = 9.29PAYY71 pKa = 9.5NRR73 pKa = 11.84NRR75 pKa = 11.84VSRR78 pKa = 11.84SGGSLLTSALSYY90 pKa = 11.09AFRR93 pKa = 11.84APPNVLICVRR103 pKa = 11.84RR104 pKa = 11.84KK105 pKa = 8.21MRR107 pKa = 11.84RR108 pKa = 11.84QVLLAKK114 pKa = 10.51GKK116 pKa = 10.41GGANRR121 pKa = 11.84RR122 pKa = 11.84GRR124 pKa = 11.84KK125 pKa = 9.22NSFSDD130 pKa = 3.77VRR132 pKa = 11.84CC133 pKa = 3.58

Molecular weight: 14.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1379

133

500

275.8

30.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.92 ± 0.668

1.088 ± 0.458

5.221 ± 0.718

5.221 ± 0.941

2.973 ± 0.342

7.179 ± 0.37

1.958 ± 0.633

4.206 ± 0.542

4.351 ± 0.816

8.702 ± 0.586

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.828 ± 0.593

3.988 ± 0.341

6.164 ± 0.736

5.149 ± 0.896

8.412 ± 1.432

7.542 ± 0.535

5.801 ± 0.641

5.004 ± 0.445

1.305 ± 0.197

3.988 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski