Novosphingobium sp. AAP1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3896 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1ALU6|A0A0N1ALU6_9SPHN Histidine kinase OS=Novosphingobium sp. AAP1 OX=1523413 GN=IP65_11340 PE=4 SV=1
MM1 pKa = 7.12RR2 pKa = 11.84TTYY5 pKa = 10.7EE6 pKa = 4.03SATVRR11 pKa = 11.84LYY13 pKa = 10.81HH14 pKa = 7.04LSDD17 pKa = 3.93GPEE20 pKa = 3.93GGAAQTLFYY29 pKa = 10.99GPLDD33 pKa = 3.47QAMALAAQQSEE44 pKa = 5.08DD45 pKa = 3.25IQADD49 pKa = 3.93LFIATDD55 pKa = 3.4NDD57 pKa = 3.61VIAWLDD63 pKa = 3.51LAEE66 pKa = 4.29GG67 pKa = 3.74

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1AML8|A0A0N1AML8_9SPHN Divalent metal cation transporter MntH OS=Novosphingobium sp. AAP1 OX=1523413 GN=mntH PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.11VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.79NLSAA44 pKa = 4.73

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3896

0

3896

1332680

29

3981

342.1

36.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.481 ± 0.071

0.795 ± 0.012

5.791 ± 0.027

4.641 ± 0.038

3.433 ± 0.021

9.008 ± 0.04

2.213 ± 0.021

4.503 ± 0.023

2.577 ± 0.029

10.209 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.019

2.502 ± 0.032

5.584 ± 0.033

3.511 ± 0.021

7.114 ± 0.038

4.822 ± 0.032

5.362 ± 0.037

7.375 ± 0.028

1.522 ± 0.019

2.188 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski