Tsukamurella phage TPA2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1B2R0|F1B2R0_9CAUD Uncharacterized protein OS=Tsukamurella phage TPA2 OX=981330 PE=4 SV=1
MM1 pKa = 7.25SRR3 pKa = 11.84VHH5 pKa = 6.85LVRR8 pKa = 11.84SNPGDD13 pKa = 3.24RR14 pKa = 11.84SYY16 pKa = 11.83AVAYY20 pKa = 9.02DD21 pKa = 3.8DD22 pKa = 4.42PLIVFPRR29 pKa = 11.84RR30 pKa = 11.84VACIHH35 pKa = 6.62SDD37 pKa = 3.23QNYY40 pKa = 9.12EE41 pKa = 4.84ISIDD45 pKa = 3.66WEE47 pKa = 3.99NTPAQLRR54 pKa = 11.84EE55 pKa = 4.15FTISSPPPMEE65 pKa = 3.9NTYY68 pKa = 10.29VVPPSGIYY76 pKa = 10.6GSGLDD81 pKa = 3.71GRR83 pKa = 11.84TIQVWASTEE92 pKa = 3.84RR93 pKa = 11.84MDD95 pKa = 5.66FEE97 pKa = 7.21DD98 pKa = 3.81ILAEE102 pKa = 4.01IGVTEE107 pKa = 4.38VLDD110 pKa = 3.95EE111 pKa = 4.65PLPVDD116 pKa = 4.52PSTLPHH122 pKa = 6.94VMGAVPTEE130 pKa = 3.79ADD132 pKa = 3.58LPEE135 pKa = 5.31DD136 pKa = 4.29FEE138 pKa = 4.9PMGVYY143 pKa = 10.45LVRR146 pKa = 11.84EE147 pKa = 4.18NGTGYY152 pKa = 10.81CNGGAGEE159 pKa = 5.18GWQSMRR165 pKa = 11.84WTPEE169 pKa = 3.51CADD172 pKa = 3.3AFGVAEE178 pKa = 4.49SDD180 pKa = 4.23VPDD183 pKa = 3.7LTVGG187 pKa = 3.38

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1B2P2|F1B2P2_9CAUD Uncharacterized protein OS=Tsukamurella phage TPA2 OX=981330 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84AQPRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84PRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84SRR19 pKa = 11.84RR20 pKa = 11.84PPPRR24 pKa = 11.84RR25 pKa = 11.84CRR27 pKa = 11.84PRR29 pKa = 11.84PCRR32 pKa = 11.84LPRR35 pKa = 11.84CPRR38 pKa = 11.84PRR40 pKa = 11.84RR41 pKa = 11.84LPRR44 pKa = 11.84PSRR47 pKa = 11.84RR48 pKa = 11.84PSPCWRR54 pKa = 11.84RR55 pKa = 11.84PARR58 pKa = 11.84AGPRR62 pKa = 11.84PRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84SPRR69 pKa = 11.84SRR71 pKa = 3.21

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

18334

49

2385

235.1

25.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.918 ± 0.432

1.004 ± 0.184

6.256 ± 0.304

5.831 ± 0.348

2.531 ± 0.191

9.256 ± 0.444

1.909 ± 0.24

4.314 ± 0.27

3.011 ± 0.254

7.571 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.149 ± 0.114

2.585 ± 0.278

6.496 ± 0.408

3.338 ± 0.146

7.26 ± 0.702

5.618 ± 0.208

7.123 ± 0.227

7.767 ± 0.226

1.92 ± 0.142

2.144 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski