Amino acid dipepetide frequency for Vibrio phage VcP032

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.533AlaAla: 9.533 ± 1.704
0.385AlaCys: 0.385 ± 0.222
4.718AlaAsp: 4.718 ± 0.655
7.029AlaGlu: 7.029 ± 0.988
4.237AlaPhe: 4.237 ± 0.554
6.452AlaGly: 6.452 ± 1.058
1.541AlaHis: 1.541 ± 0.318
5.681AlaIle: 5.681 ± 0.809
5.585AlaLys: 5.585 ± 0.688
7.8AlaLeu: 7.8 ± 0.809
3.563AlaMet: 3.563 ± 0.625
4.429AlaAsn: 4.429 ± 0.7
2.022AlaPro: 2.022 ± 0.457
4.141AlaGln: 4.141 ± 0.602
3.659AlaArg: 3.659 ± 0.695
6.066AlaSer: 6.066 ± 0.904
5.681AlaThr: 5.681 ± 0.756
5.392AlaVal: 5.392 ± 0.625
1.541AlaTrp: 1.541 ± 0.347
2.889AlaTyr: 2.889 ± 0.565
0.0AlaXaa: 0.0 ± 0.0
Cys
0.77CysAla: 0.77 ± 0.314
0.385CysCys: 0.385 ± 0.239
0.77CysAsp: 0.77 ± 0.252
1.156CysGlu: 1.156 ± 0.299
0.289CysPhe: 0.289 ± 0.204
0.578CysGly: 0.578 ± 0.277
0.289CysHis: 0.289 ± 0.17
0.289CysIle: 0.289 ± 0.187
0.578CysLys: 0.578 ± 0.284
0.77CysLeu: 0.77 ± 0.308
0.193CysMet: 0.193 ± 0.137
0.289CysAsn: 0.289 ± 0.228
0.481CysPro: 0.481 ± 0.21
1.059CysGln: 1.059 ± 0.39
0.578CysArg: 0.578 ± 0.249
0.867CysSer: 0.867 ± 0.307
0.578CysThr: 0.578 ± 0.266
0.578CysVal: 0.578 ± 0.198
0.096CysTrp: 0.096 ± 0.092
0.674CysTyr: 0.674 ± 0.284
0.0CysXaa: 0.0 ± 0.0
Asp
3.852AspAla: 3.852 ± 0.536
0.578AspCys: 0.578 ± 0.235
2.504AspAsp: 2.504 ± 0.524
4.622AspGlu: 4.622 ± 0.798
2.311AspPhe: 2.311 ± 0.526
4.237AspGly: 4.237 ± 0.669
0.867AspHis: 0.867 ± 0.229
4.622AspIle: 4.622 ± 0.543
3.274AspLys: 3.274 ± 0.617
3.852AspLeu: 3.852 ± 0.715
1.926AspMet: 1.926 ± 0.425
2.407AspAsn: 2.407 ± 0.521
3.178AspPro: 3.178 ± 0.721
2.215AspGln: 2.215 ± 0.404
2.118AspArg: 2.118 ± 0.435
2.696AspSer: 2.696 ± 0.342
2.311AspThr: 2.311 ± 0.566
4.526AspVal: 4.526 ± 0.664
0.674AspTrp: 0.674 ± 0.217
2.6AspTyr: 2.6 ± 0.479
0.0AspXaa: 0.0 ± 0.0
Glu
6.355GluAla: 6.355 ± 0.816
1.444GluCys: 1.444 ± 0.421
3.467GluAsp: 3.467 ± 0.702
3.178GluGlu: 3.178 ± 0.612
2.696GluPhe: 2.696 ± 0.373
3.081GluGly: 3.081 ± 0.73
2.022GluHis: 2.022 ± 0.516
3.755GluIle: 3.755 ± 0.565
4.237GluLys: 4.237 ± 0.847
9.244GluLeu: 9.244 ± 0.883
1.926GluMet: 1.926 ± 0.561
2.696GluAsn: 2.696 ± 0.568
2.311GluPro: 2.311 ± 0.571
5.104GluGln: 5.104 ± 0.664
4.718GluArg: 4.718 ± 0.718
4.622GluSer: 4.622 ± 0.725
3.274GluThr: 3.274 ± 0.725
5.007GluVal: 5.007 ± 0.696
1.444GluTrp: 1.444 ± 0.35
1.541GluTyr: 1.541 ± 0.342
0.0GluXaa: 0.0 ± 0.0
Phe
3.852PheAla: 3.852 ± 0.588
0.385PheCys: 0.385 ± 0.198
2.6PheAsp: 2.6 ± 0.546
3.274PheGlu: 3.274 ± 0.414
1.348PhePhe: 1.348 ± 0.398
2.407PheGly: 2.407 ± 0.621
0.096PheHis: 0.096 ± 0.092
1.733PheIle: 1.733 ± 0.496
2.696PheLys: 2.696 ± 0.539
2.215PheLeu: 2.215 ± 0.494
1.444PheMet: 1.444 ± 0.368
1.926PheAsn: 1.926 ± 0.402
0.963PhePro: 0.963 ± 0.221
1.637PheGln: 1.637 ± 0.379
1.541PheArg: 1.541 ± 0.34
2.985PheSer: 2.985 ± 0.757
2.696PheThr: 2.696 ± 0.389
2.985PheVal: 2.985 ± 0.604
0.963PheTrp: 0.963 ± 0.278
0.963PheTyr: 0.963 ± 0.382
0.0PheXaa: 0.0 ± 0.0
Gly
4.333GlyAla: 4.333 ± 0.682
0.77GlyCys: 0.77 ± 0.297
3.755GlyAsp: 3.755 ± 0.466
4.429GlyGlu: 4.429 ± 0.522
2.118GlyPhe: 2.118 ± 0.441
4.044GlyGly: 4.044 ± 0.632
1.926GlyHis: 1.926 ± 0.436
4.237GlyIle: 4.237 ± 0.671
4.718GlyLys: 4.718 ± 0.523
5.007GlyLeu: 5.007 ± 0.818
1.83GlyMet: 1.83 ± 0.635
2.311GlyAsn: 2.311 ± 0.544
0.77GlyPro: 0.77 ± 0.263
2.889GlyGln: 2.889 ± 0.581
3.755GlyArg: 3.755 ± 0.762
3.755GlySer: 3.755 ± 0.517
2.792GlyThr: 2.792 ± 0.45
4.141GlyVal: 4.141 ± 0.797
1.059GlyTrp: 1.059 ± 0.297
1.83GlyTyr: 1.83 ± 0.468
0.0GlyXaa: 0.0 ± 0.0
His
1.444HisAla: 1.444 ± 0.409
0.289HisCys: 0.289 ± 0.176
0.674HisAsp: 0.674 ± 0.309
1.541HisGlu: 1.541 ± 0.357
0.963HisPhe: 0.963 ± 0.378
1.733HisGly: 1.733 ± 0.34
0.578HisHis: 0.578 ± 0.254
1.252HisIle: 1.252 ± 0.484
1.252HisLys: 1.252 ± 0.345
1.541HisLeu: 1.541 ± 0.362
0.674HisMet: 0.674 ± 0.261
0.674HisAsn: 0.674 ± 0.206
0.867HisPro: 0.867 ± 0.33
1.156HisGln: 1.156 ± 0.293
0.385HisArg: 0.385 ± 0.17
1.156HisSer: 1.156 ± 0.346
1.156HisThr: 1.156 ± 0.33
1.252HisVal: 1.252 ± 0.317
0.385HisTrp: 0.385 ± 0.216
0.77HisTyr: 0.77 ± 0.31
0.0HisXaa: 0.0 ± 0.0
Ile
7.8IleAla: 7.8 ± 0.875
0.674IleCys: 0.674 ± 0.277
4.141IleAsp: 4.141 ± 0.677
6.066IleGlu: 6.066 ± 0.961
2.504IlePhe: 2.504 ± 0.45
3.081IleGly: 3.081 ± 0.547
1.059IleHis: 1.059 ± 0.354
2.985IleIle: 2.985 ± 0.544
3.563IleLys: 3.563 ± 0.694
3.178IleLeu: 3.178 ± 0.649
1.926IleMet: 1.926 ± 0.488
3.37IleAsn: 3.37 ± 0.633
3.178IlePro: 3.178 ± 0.811
2.118IleGln: 2.118 ± 0.365
2.889IleArg: 2.889 ± 0.788
4.815IleSer: 4.815 ± 0.795
4.044IleThr: 4.044 ± 0.639
2.215IleVal: 2.215 ± 0.35
0.77IleTrp: 0.77 ± 0.249
1.733IleTyr: 1.733 ± 0.419
0.0IleXaa: 0.0 ± 0.0
Lys
6.933LysAla: 6.933 ± 0.899
0.289LysCys: 0.289 ± 0.161
2.696LysAsp: 2.696 ± 0.607
4.141LysGlu: 4.141 ± 0.58
1.926LysPhe: 1.926 ± 0.449
3.659LysGly: 3.659 ± 0.781
1.059LysHis: 1.059 ± 0.341
3.563LysIle: 3.563 ± 0.525
3.755LysLys: 3.755 ± 0.707
5.681LysLeu: 5.681 ± 0.773
1.348LysMet: 1.348 ± 0.346
2.985LysAsn: 2.985 ± 0.577
3.37LysPro: 3.37 ± 0.5
3.467LysGln: 3.467 ± 0.51
4.815LysArg: 4.815 ± 0.809
2.985LysSer: 2.985 ± 0.571
3.274LysThr: 3.274 ± 0.597
3.948LysVal: 3.948 ± 0.564
0.77LysTrp: 0.77 ± 0.255
2.022LysTyr: 2.022 ± 0.467
0.0LysXaa: 0.0 ± 0.0
Leu
7.896LeuAla: 7.896 ± 0.906
1.059LeuCys: 1.059 ± 0.294
4.429LeuAsp: 4.429 ± 0.456
6.163LeuGlu: 6.163 ± 0.729
3.37LeuPhe: 3.37 ± 0.491
5.296LeuGly: 5.296 ± 0.652
0.867LeuHis: 0.867 ± 0.26
5.681LeuIle: 5.681 ± 0.603
6.74LeuLys: 6.74 ± 0.927
6.355LeuLeu: 6.355 ± 0.764
2.311LeuMet: 2.311 ± 0.47
4.237LeuAsn: 4.237 ± 0.799
5.007LeuPro: 5.007 ± 0.803
3.37LeuGln: 3.37 ± 0.461
4.526LeuArg: 4.526 ± 0.505
7.318LeuSer: 7.318 ± 0.854
5.489LeuThr: 5.489 ± 0.722
3.755LeuVal: 3.755 ± 0.484
0.674LeuTrp: 0.674 ± 0.228
1.733LeuTyr: 1.733 ± 0.401
0.0LeuXaa: 0.0 ± 0.0
Met
3.659MetAla: 3.659 ± 0.588
0.481MetCys: 0.481 ± 0.22
0.867MetAsp: 0.867 ± 0.328
1.059MetGlu: 1.059 ± 0.336
0.867MetPhe: 0.867 ± 0.302
1.541MetGly: 1.541 ± 0.54
0.674MetHis: 0.674 ± 0.19
1.733MetIle: 1.733 ± 0.394
0.867MetLys: 0.867 ± 0.305
2.6MetLeu: 2.6 ± 0.575
0.867MetMet: 0.867 ± 0.325
1.637MetAsn: 1.637 ± 0.328
0.867MetPro: 0.867 ± 0.342
1.348MetGln: 1.348 ± 0.358
1.541MetArg: 1.541 ± 0.333
2.985MetSer: 2.985 ± 0.488
1.733MetThr: 1.733 ± 0.52
2.118MetVal: 2.118 ± 0.357
0.289MetTrp: 0.289 ± 0.162
0.481MetTyr: 0.481 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
4.044AsnAla: 4.044 ± 0.728
0.481AsnCys: 0.481 ± 0.176
2.6AsnAsp: 2.6 ± 0.451
2.889AsnGlu: 2.889 ± 0.459
1.252AsnPhe: 1.252 ± 0.319
3.178AsnGly: 3.178 ± 0.709
0.867AsnHis: 0.867 ± 0.269
2.889AsnIle: 2.889 ± 0.501
2.985AsnLys: 2.985 ± 0.637
3.563AsnLeu: 3.563 ± 0.577
1.252AsnMet: 1.252 ± 0.319
1.733AsnAsn: 1.733 ± 0.361
2.6AsnPro: 2.6 ± 0.347
2.792AsnGln: 2.792 ± 0.539
2.504AsnArg: 2.504 ± 0.5
2.118AsnSer: 2.118 ± 0.474
2.022AsnThr: 2.022 ± 0.41
1.926AsnVal: 1.926 ± 0.4
0.481AsnTrp: 0.481 ± 0.207
1.156AsnTyr: 1.156 ± 0.35
0.0AsnXaa: 0.0 ± 0.0
Pro
3.852ProAla: 3.852 ± 0.52
0.289ProCys: 0.289 ± 0.169
3.081ProAsp: 3.081 ± 0.456
4.526ProGlu: 4.526 ± 0.714
1.926ProPhe: 1.926 ± 0.46
1.637ProGly: 1.637 ± 0.45
0.867ProHis: 0.867 ± 0.277
2.6ProIle: 2.6 ± 0.536
2.022ProLys: 2.022 ± 0.444
3.081ProLeu: 3.081 ± 0.493
1.252ProMet: 1.252 ± 0.383
1.926ProAsn: 1.926 ± 0.465
1.156ProPro: 1.156 ± 0.35
2.407ProGln: 2.407 ± 0.504
2.311ProArg: 2.311 ± 0.441
2.022ProSer: 2.022 ± 0.477
2.118ProThr: 2.118 ± 0.435
2.792ProVal: 2.792 ± 0.572
0.578ProTrp: 0.578 ± 0.251
1.541ProTyr: 1.541 ± 0.332
0.0ProXaa: 0.0 ± 0.0
Gln
4.526GlnAla: 4.526 ± 0.534
0.481GlnCys: 0.481 ± 0.211
2.118GlnAsp: 2.118 ± 0.458
2.311GlnGlu: 2.311 ± 0.603
1.83GlnPhe: 1.83 ± 0.406
3.274GlnGly: 3.274 ± 0.423
0.867GlnHis: 0.867 ± 0.301
2.889GlnIle: 2.889 ± 0.59
2.889GlnLys: 2.889 ± 0.568
4.815GlnLeu: 4.815 ± 0.486
1.637GlnMet: 1.637 ± 0.394
1.926GlnAsn: 1.926 ± 0.447
2.6GlnPro: 2.6 ± 0.445
4.237GlnGln: 4.237 ± 0.715
3.081GlnArg: 3.081 ± 0.571
2.6GlnSer: 2.6 ± 0.432
2.696GlnThr: 2.696 ± 0.532
3.659GlnVal: 3.659 ± 0.594
0.77GlnTrp: 0.77 ± 0.354
1.444GlnTyr: 1.444 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
3.755ArgAla: 3.755 ± 0.599
0.77ArgCys: 0.77 ± 0.342
2.792ArgAsp: 2.792 ± 0.586
4.237ArgGlu: 4.237 ± 0.803
2.407ArgPhe: 2.407 ± 0.488
1.926ArgGly: 1.926 ± 0.549
1.156ArgHis: 1.156 ± 0.319
3.467ArgIle: 3.467 ± 0.521
4.718ArgLys: 4.718 ± 0.97
6.644ArgLeu: 6.644 ± 0.801
0.867ArgMet: 0.867 ± 0.277
2.118ArgAsn: 2.118 ± 0.486
1.444ArgPro: 1.444 ± 0.368
2.022ArgGln: 2.022 ± 0.506
2.311ArgArg: 2.311 ± 0.435
2.792ArgSer: 2.792 ± 0.469
2.504ArgThr: 2.504 ± 0.497
2.6ArgVal: 2.6 ± 0.435
0.867ArgTrp: 0.867 ± 0.331
1.348ArgTyr: 1.348 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
6.355SerAla: 6.355 ± 0.742
0.578SerCys: 0.578 ± 0.213
4.429SerAsp: 4.429 ± 0.641
5.104SerGlu: 5.104 ± 0.839
2.215SerPhe: 2.215 ± 0.456
5.007SerGly: 5.007 ± 0.672
1.444SerHis: 1.444 ± 0.329
3.948SerIle: 3.948 ± 0.665
3.178SerLys: 3.178 ± 0.576
5.2SerLeu: 5.2 ± 0.889
1.252SerMet: 1.252 ± 0.384
2.311SerAsn: 2.311 ± 0.509
2.985SerPro: 2.985 ± 0.474
2.792SerGln: 2.792 ± 0.52
2.407SerArg: 2.407 ± 0.402
3.659SerSer: 3.659 ± 0.563
3.852SerThr: 3.852 ± 0.64
3.948SerVal: 3.948 ± 0.692
0.867SerTrp: 0.867 ± 0.27
2.118SerTyr: 2.118 ± 0.409
0.0SerXaa: 0.0 ± 0.0
Thr
4.622ThrAla: 4.622 ± 0.548
0.77ThrCys: 0.77 ± 0.339
3.755ThrAsp: 3.755 ± 0.675
3.755ThrGlu: 3.755 ± 0.649
2.311ThrPhe: 2.311 ± 0.463
3.659ThrGly: 3.659 ± 0.641
1.252ThrHis: 1.252 ± 0.293
3.274ThrIle: 3.274 ± 0.453
3.948ThrLys: 3.948 ± 0.637
4.333ThrLeu: 4.333 ± 0.697
0.963ThrMet: 0.963 ± 0.275
2.118ThrAsn: 2.118 ± 0.419
3.659ThrPro: 3.659 ± 0.591
2.985ThrGln: 2.985 ± 0.563
1.926ThrArg: 1.926 ± 0.462
3.178ThrSer: 3.178 ± 0.576
3.178ThrThr: 3.178 ± 0.412
3.081ThrVal: 3.081 ± 0.694
1.444ThrTrp: 1.444 ± 0.369
1.156ThrTyr: 1.156 ± 0.425
0.0ThrXaa: 0.0 ± 0.0
Val
5.585ValAla: 5.585 ± 0.746
0.481ValCys: 0.481 ± 0.203
3.467ValAsp: 3.467 ± 0.549
3.755ValGlu: 3.755 ± 0.601
1.926ValPhe: 1.926 ± 0.348
3.659ValGly: 3.659 ± 0.713
1.059ValHis: 1.059 ± 0.307
4.526ValIle: 4.526 ± 0.633
3.081ValLys: 3.081 ± 0.568
5.2ValLeu: 5.2 ± 0.701
1.733ValMet: 1.733 ± 0.307
2.504ValAsn: 2.504 ± 0.425
2.407ValPro: 2.407 ± 0.473
1.926ValGln: 1.926 ± 0.419
2.792ValArg: 2.792 ± 0.52
4.911ValSer: 4.911 ± 0.544
4.141ValThr: 4.141 ± 0.698
3.948ValVal: 3.948 ± 0.693
0.674ValTrp: 0.674 ± 0.2
2.504ValTyr: 2.504 ± 0.488
0.0ValXaa: 0.0 ± 0.0
Trp
1.252TrpAla: 1.252 ± 0.38
0.385TrpCys: 0.385 ± 0.181
1.252TrpAsp: 1.252 ± 0.401
0.481TrpGlu: 0.481 ± 0.178
0.674TrpPhe: 0.674 ± 0.313
0.193TrpGly: 0.193 ± 0.133
0.578TrpHis: 0.578 ± 0.238
1.059TrpIle: 1.059 ± 0.261
0.481TrpLys: 0.481 ± 0.214
1.637TrpLeu: 1.637 ± 0.404
0.385TrpMet: 0.385 ± 0.18
0.578TrpAsn: 0.578 ± 0.186
0.77TrpPro: 0.77 ± 0.239
1.348TrpGln: 1.348 ± 0.32
1.156TrpArg: 1.156 ± 0.436
0.578TrpSer: 0.578 ± 0.211
0.674TrpThr: 0.674 ± 0.299
1.252TrpVal: 1.252 ± 0.313
0.578TrpTrp: 0.578 ± 0.245
0.289TrpTyr: 0.289 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.926TyrAla: 1.926 ± 0.421
0.289TyrCys: 0.289 ± 0.175
1.252TyrAsp: 1.252 ± 0.35
2.407TyrGlu: 2.407 ± 0.576
1.541TyrPhe: 1.541 ± 0.328
1.83TyrGly: 1.83 ± 0.382
0.674TyrHis: 0.674 ± 0.258
1.926TyrIle: 1.926 ± 0.541
2.215TyrLys: 2.215 ± 0.369
3.563TyrLeu: 3.563 ± 0.654
0.77TyrMet: 0.77 ± 0.271
1.156TyrAsn: 1.156 ± 0.341
1.348TyrPro: 1.348 ± 0.402
1.637TyrGln: 1.637 ± 0.378
1.733TyrArg: 1.733 ± 0.324
1.541TyrSer: 1.541 ± 0.422
1.156TyrThr: 1.156 ± 0.399
1.156TyrVal: 1.156 ± 0.348
0.674TyrTrp: 0.674 ± 0.238
0.289TyrTyr: 0.289 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (10386 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski