Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes; Actinoplanes missouriensis

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0H5B9|I0H5B9_ACTM4 HintN domain-containing protein OS=Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431) OX=512565 GN=AMIS_29860 PE=4 SV=1
MM1 pKa = 7.26NVYY4 pKa = 10.3DD5 pKa = 3.47IAGRR9 pKa = 11.84LPSIPVLRR17 pKa = 11.84DD18 pKa = 2.87RR19 pKa = 11.84CRR21 pKa = 11.84ALAVLEE27 pKa = 4.91LIQGTGYY34 pKa = 10.9EE35 pKa = 4.5YY36 pKa = 11.2YY37 pKa = 10.62SYY39 pKa = 9.63TAWGDD44 pKa = 3.55SEE46 pKa = 4.44AALMSNGSGDD56 pKa = 3.43EE57 pKa = 4.01YY58 pKa = 10.97TIVFGDD64 pKa = 3.19AGVFIRR70 pKa = 11.84LFDD73 pKa = 4.14HH74 pKa = 6.8EE75 pKa = 4.99SAMSPYY81 pKa = 10.84AADD84 pKa = 4.78DD85 pKa = 5.99DD86 pKa = 4.19MALWPGLLDD95 pKa = 3.76GLPPEE100 pKa = 4.8FAGQVADD107 pKa = 4.52PAFCDD112 pKa = 3.91GDD114 pKa = 4.44DD115 pKa = 5.04LIATAVLWRR124 pKa = 11.84RR125 pKa = 11.84TGDD128 pKa = 3.82DD129 pKa = 3.0RR130 pKa = 11.84WHH132 pKa = 6.85AGAGITFPPARR143 pKa = 11.84GPYY146 pKa = 9.8DD147 pKa = 3.56ISPDD151 pKa = 3.31GTDD154 pKa = 3.67RR155 pKa = 11.84LSILCDD161 pKa = 4.78DD162 pKa = 4.23IVDD165 pKa = 4.34AYY167 pKa = 11.76VDD169 pKa = 4.25FASDD173 pKa = 3.93YY174 pKa = 11.04YY175 pKa = 10.11EE176 pKa = 4.1TEE178 pKa = 3.59IDD180 pKa = 3.71RR181 pKa = 11.84DD182 pKa = 3.48AVAYY186 pKa = 9.62VAALRR191 pKa = 11.84PLTDD195 pKa = 3.42DD196 pKa = 3.59VVRR199 pKa = 11.84ALNPEE204 pKa = 3.89SSLAAVRR211 pKa = 11.84TEE213 pKa = 4.06VAAAGYY219 pKa = 8.62PIATAVSS226 pKa = 3.52

Molecular weight: 24.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0HE95|I0HE95_ACTM4 Putative alcohol dehydrogenase OS=Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431) OX=512565 GN=AMIS_61120 PE=3 SV=1
MM1 pKa = 7.14VATAIAVVTRR11 pKa = 11.84VAATGAGSVAVTVTVVVTRR30 pKa = 11.84VAATGAVSVVGTVRR44 pKa = 11.84RR45 pKa = 11.84ATVVVSVVTVRR56 pKa = 11.84RR57 pKa = 11.84VTVRR61 pKa = 11.84RR62 pKa = 11.84VAASVVATVIVAATRR77 pKa = 11.84VAATAVVSVVATVIVAVTRR96 pKa = 11.84VAATAVVSVVATVTGAAIRR115 pKa = 11.84VAATGVVSVVTVRR128 pKa = 11.84RR129 pKa = 11.84GAASVVATVTVAVTRR144 pKa = 11.84VAATAVVSVVATVTVVVSRR163 pKa = 11.84VAATAVVSVVGTVRR177 pKa = 11.84RR178 pKa = 11.84ATAVVSVVTVRR189 pKa = 11.84RR190 pKa = 11.84VTVRR194 pKa = 11.84RR195 pKa = 11.84VAASVAEE202 pKa = 4.29TVTVAVTRR210 pKa = 11.84VAATAVVSVVGTVRR224 pKa = 11.84RR225 pKa = 11.84ATAVVSVVTVRR236 pKa = 11.84RR237 pKa = 11.84VTVRR241 pKa = 11.84RR242 pKa = 11.84VAASVAEE249 pKa = 4.07TAIAVATRR257 pKa = 11.84VAATAAVSAVTGRR270 pKa = 11.84RR271 pKa = 11.84VTGAATRR278 pKa = 11.84VAATAAGSVAGTVTVAVTRR297 pKa = 11.84VAATAAASVAGTVRR311 pKa = 11.84VVRR314 pKa = 11.84VRR316 pKa = 11.84ASRR319 pKa = 11.84ATAAGSVVATVRR331 pKa = 11.84RR332 pKa = 11.84VTVRR336 pKa = 11.84RR337 pKa = 11.84AAASVAEE344 pKa = 4.33TATAVATRR352 pKa = 11.84VAATAAASVVTVRR365 pKa = 11.84RR366 pKa = 11.84VTVRR370 pKa = 11.84RR371 pKa = 11.84VAASVVATATAVATRR386 pKa = 11.84VAATGAGSVVATVTGAATRR405 pKa = 11.84VAATAGATRR414 pKa = 11.84VATVVVTRR422 pKa = 11.84VAATGAGSATTTAVATGGTSGTAPVRR448 pKa = 11.84VRR450 pKa = 11.84RR451 pKa = 11.84TRR453 pKa = 11.84PARR456 pKa = 11.84RR457 pKa = 11.84RR458 pKa = 11.84SSPRR462 pKa = 11.84TFPRR466 pKa = 11.84TSSRR470 pKa = 11.84PTSTRR475 pKa = 11.84RR476 pKa = 11.84SARR479 pKa = 11.84NN480 pKa = 3.02

Molecular weight: 46.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8113

0

8113

2638011

30

4154

325.2

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.932 ± 0.047

0.692 ± 0.007

5.96 ± 0.021

5.234 ± 0.028

2.789 ± 0.017

9.26 ± 0.028

2.081 ± 0.013

3.744 ± 0.017

1.953 ± 0.021

10.312 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.775 ± 0.009

1.93 ± 0.016

6.012 ± 0.029

2.768 ± 0.014

7.894 ± 0.03

5.15 ± 0.021

6.242 ± 0.027

8.65 ± 0.023

1.574 ± 0.013

2.048 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski