Penicillium digitatum (strain PHI26 / CECT 20796) (Green mold)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium; Penicillium digitatum

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9G177|K9G177_PEND2 Rho guanyl nucleotide exchange factor putative OS=Penicillium digitatum (strain PHI26 / CECT 20796) OX=1170229 GN=PDIG_75300 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.39SFSPVALALMAAFGMVAADD21 pKa = 3.86TAASVSDD28 pKa = 4.16CASMCLSNMNAQASQLGCNSGDD50 pKa = 3.73LACLCKK56 pKa = 10.18SDD58 pKa = 3.69NYY60 pKa = 11.05KK61 pKa = 10.75FGIRR65 pKa = 11.84DD66 pKa = 3.82CTKK69 pKa = 9.63EE70 pKa = 4.02ACPSDD75 pKa = 5.06DD76 pKa = 3.99PEE78 pKa = 4.23QVLAMAVAKK87 pKa = 10.69CPGGVAGSDD96 pKa = 3.39TSSSSVSGSEE106 pKa = 4.05SSSTSSSDD114 pKa = 3.12SATTSGSDD122 pKa = 3.38STATSSDD129 pKa = 2.99ASATSTGAAVSTATGTDD146 pKa = 3.29VSVTSTDD153 pKa = 3.22ASGSKK158 pKa = 10.13AATGTDD164 pKa = 3.52VSATGTDD171 pKa = 3.39ASGSGSGASGSATSTGSVSTITSTDD196 pKa = 3.02ASSSMVTKK204 pKa = 10.74ASTGASSTSTGSSGSGSEE222 pKa = 4.2SGSGSGSGSSSDD234 pKa = 3.68SDD236 pKa = 3.76SSTSSSTAGAAASTTSHH253 pKa = 6.28GAAAAMATAGTGAMGVLGMLAIFALL278 pKa = 4.2

Molecular weight: 25.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9H0G0|K9H0G0_PEND2 Transcription factor BYE1 OS=Penicillium digitatum (strain PHI26 / CECT 20796) OX=1170229 GN=PDIG_07750 PE=3 SV=1
MM1 pKa = 7.91PKK3 pKa = 10.03RR4 pKa = 11.84PFGYY8 pKa = 9.73RR9 pKa = 11.84CSDD12 pKa = 3.62LLFPSFKK19 pKa = 10.86SLFNPPRR26 pKa = 11.84LILASNRR33 pKa = 11.84IVLCHH38 pKa = 6.81KK39 pKa = 9.63IWCHH43 pKa = 4.94RR44 pKa = 11.84LFMIFHH50 pKa = 6.7RR51 pKa = 11.84HH52 pKa = 4.68WSSIPIDD59 pKa = 3.54RR60 pKa = 11.84LHH62 pKa = 7.25RR63 pKa = 11.84SQAFLFDD70 pKa = 3.77YY71 pKa = 10.98SKK73 pKa = 11.58LCIFSIWHH81 pKa = 5.05SQIFAYY87 pKa = 8.97LTACRR92 pKa = 11.84STRR95 pKa = 3.23

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9101

0

9101

4201956

29

7066

461.7

51.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.351 ± 0.022

1.163 ± 0.009

5.685 ± 0.026

6.284 ± 0.028

3.763 ± 0.015

6.654 ± 0.022

2.44 ± 0.012

5.05 ± 0.017

4.816 ± 0.022

8.953 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.01

3.763 ± 0.011

6.1 ± 0.032

4.089 ± 0.018

6.069 ± 0.025

8.412 ± 0.034

5.989 ± 0.02

6.118 ± 0.02

1.405 ± 0.009

2.688 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski