Streptococcus satellite phage Javan298

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 5.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIT3|A0A4D5ZIT3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan298 OX=2558617 GN=JavanS298_0008 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.3IKK4 pKa = 10.74LFYY7 pKa = 10.44QKK9 pKa = 10.61YY10 pKa = 8.31KK11 pKa = 10.71QSLEE15 pKa = 4.1DD16 pKa = 4.85FEE18 pKa = 5.91SQVNDD23 pKa = 3.44FMATVEE29 pKa = 4.3VVDD32 pKa = 3.98VKK34 pKa = 11.25YY35 pKa = 11.23SEE37 pKa = 4.27ATVGNSDD44 pKa = 5.52DD45 pKa = 4.23MDD47 pKa = 4.02TLTSVMVLYY56 pKa = 10.56KK57 pKa = 10.78

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZKM3|A0A4D5ZKM3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan298 OX=2558617 GN=JavanS298_0014 PE=4 SV=1
MM1 pKa = 7.86LITSTQAKK9 pKa = 9.74AIRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.66QADD17 pKa = 3.4KK18 pKa = 11.27KK19 pKa = 10.16LTAKK23 pKa = 10.18QAGEE27 pKa = 4.41EE28 pKa = 3.96IGVTQVTYY36 pKa = 10.88RR37 pKa = 11.84KK38 pKa = 9.3IRR40 pKa = 11.84DD41 pKa = 3.51GGEE44 pKa = 4.09VKK46 pKa = 10.52PSIYY50 pKa = 9.91QKK52 pKa = 11.14AMQWLAEE59 pKa = 4.48DD60 pKa = 4.07YY61 pKa = 11.23

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

2554

39

508

141.9

16.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.756 ± 0.398

0.352 ± 0.115

6.578 ± 0.472

9.514 ± 1.048

4.307 ± 0.495

4.581 ± 0.312

1.527 ± 0.33

6.578 ± 0.452

9.593 ± 0.604

9.984 ± 0.454

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.423

5.795 ± 0.628

1.84 ± 0.21

4.464 ± 0.329

4.777 ± 0.239

4.973 ± 0.335

5.247 ± 0.646

5.521 ± 0.499

1.057 ± 0.178

4.816 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski