Microcystis phage MaeS

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5CD05|A0A6M5CD05_9CAUD Uncharacterized protein OS=Microcystis phage MaeS OX=2736190 PE=4 SV=1
MM1 pKa = 7.34NPVKK5 pKa = 10.08PYY7 pKa = 10.65SDD9 pKa = 3.68EE10 pKa = 4.12EE11 pKa = 4.12YY12 pKa = 11.3AEE14 pKa = 4.9AKK16 pKa = 9.6QQGLDD21 pKa = 3.31LDD23 pKa = 3.73NWNDD27 pKa = 3.29YY28 pKa = 10.68VKK30 pKa = 10.94YY31 pKa = 10.49FGIGEE36 pKa = 4.14DD37 pKa = 3.71EE38 pKa = 4.46EE39 pKa = 5.23YY40 pKa = 10.8EE41 pKa = 4.08

Molecular weight: 4.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5CD72|A0A6M5CD72_9CAUD Uncharacterized protein OS=Microcystis phage MaeS OX=2736190 PE=4 SV=1
MM1 pKa = 7.96QEE3 pKa = 3.63EE4 pKa = 4.89TFNHH8 pKa = 5.65SLINRR13 pKa = 11.84RR14 pKa = 11.84SEE16 pKa = 3.57HH17 pKa = 5.59ARR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 8.67EE22 pKa = 3.71EE23 pKa = 4.35RR24 pKa = 11.84IKK26 pKa = 8.69QTRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84GFRR34 pKa = 11.84CSSVSGSNSFKK45 pKa = 9.9KK46 pKa = 9.26TPAAKK51 pKa = 10.05DD52 pKa = 3.54FYY54 pKa = 10.87INVHH58 pKa = 6.52LYY60 pKa = 9.57RR61 pKa = 11.84KK62 pKa = 9.56GYY64 pKa = 10.3KK65 pKa = 9.72SLLQIQSKK73 pKa = 10.39

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

113

0

113

23797

37

1784

210.6

23.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.27 ± 0.42

0.933 ± 0.128

5.955 ± 0.184

8.039 ± 0.376

4.013 ± 0.17

6.379 ± 0.278

1.752 ± 0.12

6.904 ± 0.25

8.472 ± 0.379

7.745 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.19

5.908 ± 0.196

2.891 ± 0.148

4.009 ± 0.261

4.501 ± 0.27

5.934 ± 0.311

6.244 ± 0.522

6.379 ± 0.194

1.114 ± 0.073

4.08 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski