Amino acid dipepetide frequency for Mycobacterium phage PainterBoy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.226AlaAla: 11.226 ± 1.041
0.797AlaCys: 0.797 ± 0.222
5.582AlaAsp: 5.582 ± 0.596
7.177AlaGlu: 7.177 ± 0.754
3.251AlaPhe: 3.251 ± 0.408
7.238AlaGly: 7.238 ± 0.823
1.472AlaHis: 1.472 ± 0.289
4.907AlaIle: 4.907 ± 0.522
5.643AlaLys: 5.643 ± 0.721
8.588AlaLeu: 8.588 ± 0.798
2.699AlaMet: 2.699 ± 0.395
2.944AlaAsn: 2.944 ± 0.402
5.275AlaPro: 5.275 ± 0.707
3.067AlaGln: 3.067 ± 0.405
5.705AlaArg: 5.705 ± 0.571
4.662AlaSer: 4.662 ± 0.544
5.091AlaThr: 5.091 ± 0.47
6.441AlaVal: 6.441 ± 0.628
1.718AlaTrp: 1.718 ± 0.268
2.638AlaTyr: 2.638 ± 0.47
0.0AlaXaa: 0.0 ± 0.0
Cys
0.675CysAla: 0.675 ± 0.193
0.0CysCys: 0.0 ± 0.0
0.859CysAsp: 0.859 ± 0.215
0.736CysGlu: 0.736 ± 0.258
0.184CysPhe: 0.184 ± 0.106
0.797CysGly: 0.797 ± 0.205
0.368CysHis: 0.368 ± 0.13
0.245CysIle: 0.245 ± 0.123
0.184CysLys: 0.184 ± 0.092
0.613CysLeu: 0.613 ± 0.217
0.184CysMet: 0.184 ± 0.104
0.429CysAsn: 0.429 ± 0.144
0.613CysPro: 0.613 ± 0.248
0.307CysGln: 0.307 ± 0.147
0.613CysArg: 0.613 ± 0.184
0.797CysSer: 0.797 ± 0.249
0.429CysThr: 0.429 ± 0.16
0.736CysVal: 0.736 ± 0.217
0.368CysTrp: 0.368 ± 0.175
0.307CysTyr: 0.307 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
5.521AspAla: 5.521 ± 0.623
0.797AspCys: 0.797 ± 0.214
3.619AspAsp: 3.619 ± 0.494
4.171AspGlu: 4.171 ± 0.455
2.27AspPhe: 2.27 ± 0.373
5.828AspGly: 5.828 ± 0.584
1.595AspHis: 1.595 ± 0.307
3.251AspIle: 3.251 ± 0.351
2.76AspLys: 2.76 ± 0.523
6.441AspLeu: 6.441 ± 0.608
1.656AspMet: 1.656 ± 0.306
1.718AspAsn: 1.718 ± 0.296
4.907AspPro: 4.907 ± 0.473
1.595AspGln: 1.595 ± 0.299
3.251AspArg: 3.251 ± 0.425
3.374AspSer: 3.374 ± 0.466
4.049AspThr: 4.049 ± 0.48
4.969AspVal: 4.969 ± 0.406
1.411AspTrp: 1.411 ± 0.303
2.454AspTyr: 2.454 ± 0.377
0.0AspXaa: 0.0 ± 0.0
Glu
8.22GluAla: 8.22 ± 0.778
0.184GluCys: 0.184 ± 0.096
4.846GluAsp: 4.846 ± 0.565
4.417GluGlu: 4.417 ± 0.644
2.147GluPhe: 2.147 ± 0.354
5.459GluGly: 5.459 ± 0.555
1.84GluHis: 1.84 ± 0.43
4.049GluIle: 4.049 ± 0.443
2.699GluLys: 2.699 ± 0.524
6.564GluLeu: 6.564 ± 0.708
1.779GluMet: 1.779 ± 0.416
2.331GluAsn: 2.331 ± 0.328
2.883GluPro: 2.883 ± 0.432
2.024GluGln: 2.024 ± 0.299
4.601GluArg: 4.601 ± 0.552
3.067GluSer: 3.067 ± 0.515
3.497GluThr: 3.497 ± 0.447
4.723GluVal: 4.723 ± 0.551
1.534GluTrp: 1.534 ± 0.274
1.779GluTyr: 1.779 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
2.883PheAla: 2.883 ± 0.372
0.429PheCys: 0.429 ± 0.168
2.392PheAsp: 2.392 ± 0.45
2.086PheGlu: 2.086 ± 0.387
0.859PhePhe: 0.859 ± 0.298
3.006PheGly: 3.006 ± 0.414
0.613PheHis: 0.613 ± 0.223
1.35PheIle: 1.35 ± 0.236
1.288PheLys: 1.288 ± 0.24
2.086PheLeu: 2.086 ± 0.327
0.797PheMet: 0.797 ± 0.232
1.534PheAsn: 1.534 ± 0.331
2.331PhePro: 2.331 ± 0.352
1.411PheGln: 1.411 ± 0.338
1.963PheArg: 1.963 ± 0.248
2.331PheSer: 2.331 ± 0.357
2.638PheThr: 2.638 ± 0.329
2.086PheVal: 2.086 ± 0.339
0.552PheTrp: 0.552 ± 0.168
0.613PheTyr: 0.613 ± 0.188
0.0PheXaa: 0.0 ± 0.0
Gly
6.441GlyAla: 6.441 ± 0.973
0.797GlyCys: 0.797 ± 0.267
5.275GlyAsp: 5.275 ± 0.649
4.907GlyGlu: 4.907 ± 0.548
3.067GlyPhe: 3.067 ± 0.485
9.324GlyGly: 9.324 ± 2.177
1.902GlyHis: 1.902 ± 0.368
3.865GlyIle: 3.865 ± 0.476
4.294GlyLys: 4.294 ± 0.443
6.748GlyLeu: 6.748 ± 0.895
1.534GlyMet: 1.534 ± 0.249
2.76GlyAsn: 2.76 ± 0.503
3.374GlyPro: 3.374 ± 0.395
2.883GlyGln: 2.883 ± 0.454
4.049GlyArg: 4.049 ± 0.382
4.785GlySer: 4.785 ± 0.626
4.969GlyThr: 4.969 ± 0.674
6.073GlyVal: 6.073 ± 0.604
1.84GlyTrp: 1.84 ± 0.293
2.27GlyTyr: 2.27 ± 0.367
0.0GlyXaa: 0.0 ± 0.0
His
1.902HisAla: 1.902 ± 0.36
0.245HisCys: 0.245 ± 0.119
1.35HisAsp: 1.35 ± 0.265
1.902HisGlu: 1.902 ± 0.394
0.613HisPhe: 0.613 ± 0.22
1.534HisGly: 1.534 ± 0.338
0.491HisHis: 0.491 ± 0.199
1.166HisIle: 1.166 ± 0.228
1.104HisLys: 1.104 ± 0.223
1.288HisLeu: 1.288 ± 0.342
0.061HisMet: 0.061 ± 0.054
0.491HisAsn: 0.491 ± 0.152
1.718HisPro: 1.718 ± 0.341
0.736HisGln: 0.736 ± 0.179
1.718HisArg: 1.718 ± 0.295
1.043HisSer: 1.043 ± 0.239
1.166HisThr: 1.166 ± 0.282
1.043HisVal: 1.043 ± 0.296
0.245HisTrp: 0.245 ± 0.129
0.491HisTyr: 0.491 ± 0.259
0.0HisXaa: 0.0 ± 0.0
Ile
5.643IleAla: 5.643 ± 0.509
0.368IleCys: 0.368 ± 0.145
4.601IleAsp: 4.601 ± 0.513
4.601IleGlu: 4.601 ± 0.428
1.472IlePhe: 1.472 ± 0.254
3.619IleGly: 3.619 ± 0.473
1.288IleHis: 1.288 ± 0.308
1.902IleIle: 1.902 ± 0.311
2.76IleLys: 2.76 ± 0.386
3.374IleLeu: 3.374 ± 0.403
0.429IleMet: 0.429 ± 0.141
2.208IleAsn: 2.208 ± 0.356
3.374IlePro: 3.374 ± 0.432
1.043IleGln: 1.043 ± 0.267
3.742IleArg: 3.742 ± 0.416
2.944IleSer: 2.944 ± 0.346
3.006IleThr: 3.006 ± 0.408
2.515IleVal: 2.515 ± 0.382
0.675IleTrp: 0.675 ± 0.197
1.104IleTyr: 1.104 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
4.969LysAla: 4.969 ± 0.647
0.245LysCys: 0.245 ± 0.103
3.006LysAsp: 3.006 ± 0.47
2.454LysGlu: 2.454 ± 0.399
1.288LysPhe: 1.288 ± 0.298
3.435LysGly: 3.435 ± 0.501
0.613LysHis: 0.613 ± 0.193
2.515LysIle: 2.515 ± 0.36
3.558LysLys: 3.558 ± 0.534
3.312LysLeu: 3.312 ± 0.44
1.35LysMet: 1.35 ± 0.276
1.043LysAsn: 1.043 ± 0.27
2.883LysPro: 2.883 ± 0.508
1.779LysGln: 1.779 ± 0.398
3.128LysArg: 3.128 ± 0.439
2.576LysSer: 2.576 ± 0.441
3.312LysThr: 3.312 ± 0.402
4.723LysVal: 4.723 ± 0.553
0.859LysTrp: 0.859 ± 0.403
1.35LysTyr: 1.35 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
8.097LeuAla: 8.097 ± 0.676
0.675LeuCys: 0.675 ± 0.225
4.478LeuAsp: 4.478 ± 0.565
5.828LeuGlu: 5.828 ± 0.818
2.638LeuPhe: 2.638 ± 0.349
5.582LeuGly: 5.582 ± 0.738
1.84LeuHis: 1.84 ± 0.396
4.171LeuIle: 4.171 ± 0.492
3.803LeuLys: 3.803 ± 0.467
4.969LeuLeu: 4.969 ± 0.591
2.331LeuMet: 2.331 ± 0.386
2.331LeuAsn: 2.331 ± 0.447
4.478LeuPro: 4.478 ± 0.481
2.638LeuGln: 2.638 ± 0.501
5.828LeuArg: 5.828 ± 0.628
5.705LeuSer: 5.705 ± 0.549
4.233LeuThr: 4.233 ± 0.478
5.521LeuVal: 5.521 ± 0.533
1.779LeuTrp: 1.779 ± 0.355
2.208LeuTyr: 2.208 ± 0.464
0.0LeuXaa: 0.0 ± 0.0
Met
2.392MetAla: 2.392 ± 0.41
0.123MetCys: 0.123 ± 0.083
1.043MetAsp: 1.043 ± 0.274
1.227MetGlu: 1.227 ± 0.214
0.429MetPhe: 0.429 ± 0.156
1.227MetGly: 1.227 ± 0.357
0.429MetHis: 0.429 ± 0.154
1.166MetIle: 1.166 ± 0.3
1.35MetLys: 1.35 ± 0.263
1.779MetLeu: 1.779 ± 0.3
0.429MetMet: 0.429 ± 0.154
0.859MetAsn: 0.859 ± 0.224
1.288MetPro: 1.288 ± 0.284
0.675MetGln: 0.675 ± 0.219
1.595MetArg: 1.595 ± 0.265
2.086MetSer: 2.086 ± 0.323
2.883MetThr: 2.883 ± 0.448
1.411MetVal: 1.411 ± 0.259
0.307MetTrp: 0.307 ± 0.129
0.613MetTyr: 0.613 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
2.944AsnAla: 2.944 ± 0.429
0.368AsnCys: 0.368 ± 0.178
1.84AsnAsp: 1.84 ± 0.315
2.638AsnGlu: 2.638 ± 0.399
1.288AsnPhe: 1.288 ± 0.316
3.435AsnGly: 3.435 ± 0.407
0.736AsnHis: 0.736 ± 0.166
1.534AsnIle: 1.534 ± 0.254
1.35AsnLys: 1.35 ± 0.29
2.576AsnLeu: 2.576 ± 0.391
0.736AsnMet: 0.736 ± 0.221
0.92AsnAsn: 0.92 ± 0.224
2.024AsnPro: 2.024 ± 0.365
1.043AsnGln: 1.043 ± 0.211
1.963AsnArg: 1.963 ± 0.387
2.27AsnSer: 2.27 ± 0.358
1.84AsnThr: 1.84 ± 0.398
2.27AsnVal: 2.27 ± 0.307
0.797AsnTrp: 0.797 ± 0.218
0.675AsnTyr: 0.675 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
5.03ProAla: 5.03 ± 0.685
0.552ProCys: 0.552 ± 0.194
4.355ProAsp: 4.355 ± 0.372
4.785ProGlu: 4.785 ± 0.477
2.024ProPhe: 2.024 ± 0.365
5.214ProGly: 5.214 ± 0.721
0.981ProHis: 0.981 ± 0.221
2.822ProIle: 2.822 ± 0.445
2.086ProLys: 2.086 ± 0.349
3.251ProLeu: 3.251 ± 0.393
0.859ProMet: 0.859 ± 0.216
2.944ProAsn: 2.944 ± 0.417
2.086ProPro: 2.086 ± 0.426
2.147ProGln: 2.147 ± 0.401
3.681ProArg: 3.681 ± 0.564
2.331ProSer: 2.331 ± 0.435
4.171ProThr: 4.171 ± 0.499
4.11ProVal: 4.11 ± 0.497
0.859ProTrp: 0.859 ± 0.347
1.472ProTyr: 1.472 ± 0.281
0.0ProXaa: 0.0 ± 0.0
Gln
4.171GlnAla: 4.171 ± 0.654
0.184GlnCys: 0.184 ± 0.103
1.534GlnAsp: 1.534 ± 0.371
1.718GlnGlu: 1.718 ± 0.318
1.166GlnPhe: 1.166 ± 0.2
2.822GlnGly: 2.822 ± 0.351
0.613GlnHis: 0.613 ± 0.185
2.515GlnIle: 2.515 ± 0.438
1.411GlnLys: 1.411 ± 0.299
2.392GlnLeu: 2.392 ± 0.424
0.859GlnMet: 0.859 ± 0.201
0.736GlnAsn: 0.736 ± 0.203
1.718GlnPro: 1.718 ± 0.33
1.902GlnGln: 1.902 ± 0.494
2.331GlnArg: 2.331 ± 0.34
1.902GlnSer: 1.902 ± 0.402
1.595GlnThr: 1.595 ± 0.307
3.19GlnVal: 3.19 ± 0.35
0.675GlnTrp: 0.675 ± 0.237
0.92GlnTyr: 0.92 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
5.337ArgAla: 5.337 ± 0.498
1.043ArgCys: 1.043 ± 0.307
3.803ArgAsp: 3.803 ± 0.5
4.539ArgGlu: 4.539 ± 0.579
2.392ArgPhe: 2.392 ± 0.427
3.987ArgGly: 3.987 ± 0.488
1.534ArgHis: 1.534 ± 0.33
3.435ArgIle: 3.435 ± 0.498
3.374ArgLys: 3.374 ± 0.455
5.828ArgLeu: 5.828 ± 0.608
2.454ArgMet: 2.454 ± 0.356
1.902ArgAsn: 1.902 ± 0.396
2.638ArgPro: 2.638 ± 0.341
1.902ArgGln: 1.902 ± 0.387
6.012ArgArg: 6.012 ± 0.806
3.558ArgSer: 3.558 ± 0.543
2.76ArgThr: 2.76 ± 0.403
4.171ArgVal: 4.171 ± 0.557
1.35ArgTrp: 1.35 ± 0.259
1.718ArgTyr: 1.718 ± 0.404
0.0ArgXaa: 0.0 ± 0.0
Ser
5.459SerAla: 5.459 ± 0.594
0.613SerCys: 0.613 ± 0.2
4.662SerAsp: 4.662 ± 0.548
3.803SerGlu: 3.803 ± 0.554
2.27SerPhe: 2.27 ± 0.381
5.459SerGly: 5.459 ± 0.672
1.043SerHis: 1.043 ± 0.264
2.76SerIle: 2.76 ± 0.386
2.822SerLys: 2.822 ± 0.462
4.601SerLeu: 4.601 ± 0.661
1.35SerMet: 1.35 ± 0.274
1.472SerAsn: 1.472 ± 0.348
3.435SerPro: 3.435 ± 0.44
2.27SerGln: 2.27 ± 0.344
4.171SerArg: 4.171 ± 0.454
3.803SerSer: 3.803 ± 0.531
3.006SerThr: 3.006 ± 0.433
3.865SerVal: 3.865 ± 0.515
1.227SerTrp: 1.227 ± 0.308
1.166SerTyr: 1.166 ± 0.316
0.0SerXaa: 0.0 ± 0.0
Thr
5.091ThrAla: 5.091 ± 0.432
0.613ThrCys: 0.613 ± 0.212
3.435ThrAsp: 3.435 ± 0.574
3.742ThrGlu: 3.742 ± 0.423
2.208ThrPhe: 2.208 ± 0.342
5.03ThrGly: 5.03 ± 0.667
1.043ThrHis: 1.043 ± 0.277
2.76ThrIle: 2.76 ± 0.41
3.19ThrLys: 3.19 ± 0.431
4.846ThrLeu: 4.846 ± 0.51
1.227ThrMet: 1.227 ± 0.362
1.718ThrAsn: 1.718 ± 0.269
4.478ThrPro: 4.478 ± 0.6
2.454ThrGln: 2.454 ± 0.35
2.883ThrArg: 2.883 ± 0.388
3.803ThrSer: 3.803 ± 0.504
3.128ThrThr: 3.128 ± 0.444
5.214ThrVal: 5.214 ± 0.66
0.92ThrTrp: 0.92 ± 0.245
1.534ThrTyr: 1.534 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
6.748ValAla: 6.748 ± 0.614
0.859ValCys: 0.859 ± 0.248
5.643ValAsp: 5.643 ± 0.606
4.785ValGlu: 4.785 ± 0.51
2.331ValPhe: 2.331 ± 0.51
5.03ValGly: 5.03 ± 0.547
0.92ValHis: 0.92 ± 0.249
4.049ValIle: 4.049 ± 0.585
3.19ValLys: 3.19 ± 0.452
5.705ValLeu: 5.705 ± 0.623
1.656ValMet: 1.656 ± 0.313
3.374ValAsn: 3.374 ± 0.629
3.987ValPro: 3.987 ± 0.576
2.086ValGln: 2.086 ± 0.334
3.926ValArg: 3.926 ± 0.499
4.969ValSer: 4.969 ± 0.626
4.049ValThr: 4.049 ± 0.412
5.766ValVal: 5.766 ± 0.746
1.411ValTrp: 1.411 ± 0.291
1.779ValTyr: 1.779 ± 0.308
0.0ValXaa: 0.0 ± 0.0
Trp
1.411TrpAla: 1.411 ± 0.34
0.307TrpCys: 0.307 ± 0.151
1.166TrpAsp: 1.166 ± 0.283
1.288TrpGlu: 1.288 ± 0.295
0.491TrpPhe: 0.491 ± 0.161
1.043TrpGly: 1.043 ± 0.279
0.675TrpHis: 0.675 ± 0.211
1.104TrpIle: 1.104 ± 0.273
0.859TrpLys: 0.859 ± 0.212
1.288TrpLeu: 1.288 ± 0.288
0.368TrpMet: 0.368 ± 0.149
0.859TrpAsn: 0.859 ± 0.217
0.92TrpPro: 0.92 ± 0.229
1.166TrpGln: 1.166 ± 0.25
0.675TrpArg: 0.675 ± 0.19
1.779TrpSer: 1.779 ± 0.342
1.35TrpThr: 1.35 ± 0.31
1.472TrpVal: 1.472 ± 0.269
0.675TrpTrp: 0.675 ± 0.22
0.491TrpTyr: 0.491 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.963TyrAla: 1.963 ± 0.268
0.245TyrCys: 0.245 ± 0.106
2.147TyrAsp: 2.147 ± 0.38
1.84TyrGlu: 1.84 ± 0.384
0.859TyrPhe: 0.859 ± 0.274
2.086TyrGly: 2.086 ± 0.378
0.307TyrHis: 0.307 ± 0.143
1.104TyrIle: 1.104 ± 0.235
0.675TyrLys: 0.675 ± 0.207
2.883TyrLeu: 2.883 ± 0.5
0.491TyrMet: 0.491 ± 0.182
0.675TyrAsn: 0.675 ± 0.203
1.35TyrPro: 1.35 ± 0.343
1.227TyrGln: 1.227 ± 0.232
1.902TyrArg: 1.902 ± 0.327
1.534TyrSer: 1.534 ± 0.275
2.147TyrThr: 2.147 ± 0.356
1.963TyrVal: 1.963 ± 0.372
0.184TyrTrp: 0.184 ± 0.104
1.043TyrTyr: 1.043 ± 0.318
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (16303 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski