Pseudomonas phage Lu11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 391 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1TQ43|I1TQ43_9CAUD Uncharacterized protein OS=Pseudomonas phage Lu11 OX=1161927 GN=Lu11_0042 PE=4 SV=1
MM1 pKa = 7.65AFGSNIYY8 pKa = 9.76VAIGATGVYY17 pKa = 9.52EE18 pKa = 4.85SSDD21 pKa = 3.16KK22 pKa = 10.99FDD24 pKa = 3.44WKK26 pKa = 9.94RR27 pKa = 11.84TSDD30 pKa = 3.53LQFSHH35 pKa = 6.43VVYY38 pKa = 10.96ANGFYY43 pKa = 10.68AVAADD48 pKa = 4.55GIYY51 pKa = 10.92VKK53 pKa = 10.82GADD56 pKa = 3.49GWALAHH62 pKa = 6.54AFVIPVVCARR72 pKa = 11.84NVNGEE77 pKa = 4.21ALFGTTQGTILRR89 pKa = 11.84SSDD92 pKa = 2.72WSEE95 pKa = 3.98VSVGLGAIWLSDD107 pKa = 3.63SSDD110 pKa = 4.6DD111 pKa = 3.87ILDD114 pKa = 3.77VLLVRR119 pKa = 11.84SDD121 pKa = 3.92YY122 pKa = 11.5VEE124 pKa = 4.25SLQGTLNNLVSTGTTEE140 pKa = 3.82IDD142 pKa = 3.37GLTVHH147 pKa = 6.97ACAYY151 pKa = 10.72DD152 pKa = 3.77DD153 pKa = 3.91VNKK156 pKa = 10.61CVVASLAFDD165 pKa = 4.32GQPMFAVLKK174 pKa = 9.16NNQWLFSIVALDD186 pKa = 3.92EE187 pKa = 4.26PAISLARR194 pKa = 11.84GAVLTSNAVYY204 pKa = 7.58TTTDD208 pKa = 3.42YY209 pKa = 11.82VSFTLLHH216 pKa = 6.21SFADD220 pKa = 5.04FEE222 pKa = 4.58AQTLAYY228 pKa = 10.26LL229 pKa = 4.08

Molecular weight: 24.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1TQW5|I1TQW5_9CAUD Putative NUDIX hydrolase protein OS=Pseudomonas phage Lu11 OX=1161927 GN=Lu11_0307 PE=4 SV=1
MM1 pKa = 7.63NINTGAPMRR10 pKa = 11.84PNRR13 pKa = 11.84LGTSKK18 pKa = 10.13VWSLVRR24 pKa = 11.84RR25 pKa = 11.84PHH27 pKa = 6.34SIAVVGLSAFMWSPVLIGYY46 pKa = 6.2QTEE49 pKa = 4.17VATVGFALSWAMQIFVGKK67 pKa = 8.44EE68 pKa = 3.12TDD70 pKa = 2.94AATRR74 pKa = 11.84KK75 pKa = 9.97LEE77 pKa = 4.07RR78 pKa = 11.84FNMCAFSLLFALLAFNVVSVV98 pKa = 3.73

Molecular weight: 10.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

391

0

391

89873

36

2020

229.9

25.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.725 ± 0.148

1.175 ± 0.065

6.194 ± 0.115

6.645 ± 0.156

4.122 ± 0.087

6.53 ± 0.169

2.278 ± 0.072

5.322 ± 0.086

6.603 ± 0.268

7.813 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.067

4.238 ± 0.09

4.628 ± 0.09

3.542 ± 0.066

5.67 ± 0.109

5.971 ± 0.106

5.766 ± 0.125

7.153 ± 0.111

1.164 ± 0.043

3.651 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski