Amino acid dipepetide frequency for Escherichia phage EP335

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.136AlaAla: 7.136 ± 1.193
0.7AlaCys: 0.7 ± 0.18
4.86AlaAsp: 4.86 ± 0.52
5.297AlaGlu: 5.297 ± 0.944
3.24AlaPhe: 3.24 ± 0.351
5.516AlaGly: 5.516 ± 0.707
1.27AlaHis: 1.27 ± 0.224
4.728AlaIle: 4.728 ± 0.488
4.597AlaLys: 4.597 ± 0.836
7.093AlaLeu: 7.093 ± 0.608
2.189AlaMet: 2.189 ± 0.327
4.203AlaAsn: 4.203 ± 0.52
2.933AlaPro: 2.933 ± 0.473
4.247AlaGln: 4.247 ± 0.64
4.159AlaArg: 4.159 ± 0.765
4.86AlaSer: 4.86 ± 0.473
4.028AlaThr: 4.028 ± 0.576
4.378AlaVal: 4.378 ± 0.449
1.007AlaTrp: 1.007 ± 0.177
2.189AlaTyr: 2.189 ± 0.342
0.0AlaXaa: 0.0 ± 0.0
Cys
0.876CysAla: 0.876 ± 0.22
0.044CysCys: 0.044 ± 0.05
0.525CysAsp: 0.525 ± 0.182
0.613CysGlu: 0.613 ± 0.172
0.35CysPhe: 0.35 ± 0.14
0.963CysGly: 0.963 ± 0.235
0.35CysHis: 0.35 ± 0.113
0.788CysIle: 0.788 ± 0.186
1.051CysLys: 1.051 ± 0.293
0.963CysLeu: 0.963 ± 0.245
0.482CysMet: 0.482 ± 0.189
0.482CysAsn: 0.482 ± 0.168
0.306CysPro: 0.306 ± 0.116
0.35CysGln: 0.35 ± 0.122
0.569CysArg: 0.569 ± 0.15
0.7CysSer: 0.7 ± 0.218
0.657CysThr: 0.657 ± 0.2
1.007CysVal: 1.007 ± 0.262
0.131CysTrp: 0.131 ± 0.082
0.525CysTyr: 0.525 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
5.21AspAla: 5.21 ± 0.489
0.7AspCys: 0.7 ± 0.177
3.108AspAsp: 3.108 ± 0.368
4.685AspGlu: 4.685 ± 0.628
2.058AspPhe: 2.058 ± 0.317
4.597AspGly: 4.597 ± 0.465
0.788AspHis: 0.788 ± 0.27
3.765AspIle: 3.765 ± 0.464
3.502AspLys: 3.502 ± 0.404
4.422AspLeu: 4.422 ± 0.364
2.145AspMet: 2.145 ± 0.389
3.546AspAsn: 3.546 ± 0.401
2.189AspPro: 2.189 ± 0.37
1.883AspGln: 1.883 ± 0.381
2.627AspArg: 2.627 ± 0.346
4.509AspSer: 4.509 ± 0.457
3.065AspThr: 3.065 ± 0.346
5.079AspVal: 5.079 ± 0.464
1.051AspTrp: 1.051 ± 0.215
2.539AspTyr: 2.539 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
6.173GluAla: 6.173 ± 0.85
0.744GluCys: 0.744 ± 0.167
4.86GluAsp: 4.86 ± 0.655
6.436GluGlu: 6.436 ± 0.758
2.627GluPhe: 2.627 ± 0.338
4.641GluGly: 4.641 ± 0.478
1.489GluHis: 1.489 ± 0.284
3.897GluIle: 3.897 ± 0.426
3.94GluLys: 3.94 ± 0.545
5.035GluLeu: 5.035 ± 0.471
2.627GluMet: 2.627 ± 0.335
2.802GluAsn: 2.802 ± 0.291
1.97GluPro: 1.97 ± 0.319
2.496GluGln: 2.496 ± 0.411
4.028GluArg: 4.028 ± 0.652
3.984GluSer: 3.984 ± 0.432
3.196GluThr: 3.196 ± 0.392
4.947GluVal: 4.947 ± 0.494
1.357GluTrp: 1.357 ± 0.28
2.277GluTyr: 2.277 ± 0.294
0.0GluXaa: 0.0 ± 0.0
Phe
2.277PheAla: 2.277 ± 0.355
0.7PheCys: 0.7 ± 0.205
3.108PheAsp: 3.108 ± 0.371
2.189PheGlu: 2.189 ± 0.369
1.401PhePhe: 1.401 ± 0.224
2.32PheGly: 2.32 ± 0.317
0.394PheHis: 0.394 ± 0.136
2.101PheIle: 2.101 ± 0.317
2.277PheLys: 2.277 ± 0.385
3.371PheLeu: 3.371 ± 0.432
0.525PheMet: 0.525 ± 0.173
3.108PheAsn: 3.108 ± 0.366
1.051PhePro: 1.051 ± 0.205
1.357PheGln: 1.357 ± 0.214
2.496PheArg: 2.496 ± 0.325
2.189PheSer: 2.189 ± 0.276
2.408PheThr: 2.408 ± 0.282
2.496PheVal: 2.496 ± 0.299
0.438PheTrp: 0.438 ± 0.145
1.97PheTyr: 1.97 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
5.823GlyAla: 5.823 ± 0.782
1.095GlyCys: 1.095 ± 0.277
4.422GlyAsp: 4.422 ± 0.381
4.115GlyGlu: 4.115 ± 0.548
3.284GlyPhe: 3.284 ± 0.296
6.523GlyGly: 6.523 ± 0.946
1.138GlyHis: 1.138 ± 0.255
3.415GlyIle: 3.415 ± 0.418
4.597GlyLys: 4.597 ± 0.556
5.254GlyLeu: 5.254 ± 0.522
2.145GlyMet: 2.145 ± 0.335
3.459GlyAsn: 3.459 ± 0.392
1.007GlyPro: 1.007 ± 0.209
2.539GlyGln: 2.539 ± 0.412
3.678GlyArg: 3.678 ± 0.402
6.129GlySer: 6.129 ± 0.679
3.459GlyThr: 3.459 ± 0.465
5.779GlyVal: 5.779 ± 0.633
0.919GlyTrp: 0.919 ± 0.186
2.539GlyTyr: 2.539 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
0.963HisAla: 0.963 ± 0.226
0.175HisCys: 0.175 ± 0.092
1.095HisAsp: 1.095 ± 0.252
1.182HisGlu: 1.182 ± 0.274
0.657HisPhe: 0.657 ± 0.182
1.007HisGly: 1.007 ± 0.23
0.35HisHis: 0.35 ± 0.114
1.401HisIle: 1.401 ± 0.27
1.182HisLys: 1.182 ± 0.252
1.226HisLeu: 1.226 ± 0.23
0.438HisMet: 0.438 ± 0.121
1.576HisAsn: 1.576 ± 0.238
0.613HisPro: 0.613 ± 0.191
0.482HisGln: 0.482 ± 0.112
1.138HisArg: 1.138 ± 0.311
1.226HisSer: 1.226 ± 0.326
0.832HisThr: 0.832 ± 0.182
1.051HisVal: 1.051 ± 0.246
0.175HisTrp: 0.175 ± 0.09
0.744HisTyr: 0.744 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
3.634IleAla: 3.634 ± 0.408
0.613IleCys: 0.613 ± 0.191
3.502IleAsp: 3.502 ± 0.399
3.94IleGlu: 3.94 ± 0.415
1.883IlePhe: 1.883 ± 0.306
3.678IleGly: 3.678 ± 0.493
0.525IleHis: 0.525 ± 0.156
3.021IleIle: 3.021 ± 0.504
3.809IleLys: 3.809 ± 0.447
3.678IleLeu: 3.678 ± 0.386
1.226IleMet: 1.226 ± 0.256
3.59IleAsn: 3.59 ± 0.453
3.809IlePro: 3.809 ± 0.427
2.101IleGln: 2.101 ± 0.33
3.108IleArg: 3.108 ± 0.378
4.641IleSer: 4.641 ± 0.444
3.853IleThr: 3.853 ± 0.543
2.977IleVal: 2.977 ± 0.511
0.876IleTrp: 0.876 ± 0.214
1.489IleTyr: 1.489 ± 0.323
0.0IleXaa: 0.0 ± 0.0
Lys
5.516LysAla: 5.516 ± 0.793
0.7LysCys: 0.7 ± 0.211
3.546LysAsp: 3.546 ± 0.404
5.473LysGlu: 5.473 ± 0.545
1.795LysPhe: 1.795 ± 0.274
4.115LysGly: 4.115 ± 0.451
1.883LysHis: 1.883 ± 0.307
3.327LysIle: 3.327 ± 0.444
4.115LysLys: 4.115 ± 0.433
4.86LysLeu: 4.86 ± 0.429
2.233LysMet: 2.233 ± 0.358
2.802LysAsn: 2.802 ± 0.433
1.751LysPro: 1.751 ± 0.332
2.802LysGln: 2.802 ± 0.394
2.627LysArg: 2.627 ± 0.387
3.634LysSer: 3.634 ± 0.469
3.546LysThr: 3.546 ± 0.467
4.466LysVal: 4.466 ± 0.47
1.095LysTrp: 1.095 ± 0.241
2.058LysTyr: 2.058 ± 0.292
0.0LysXaa: 0.0 ± 0.0
Leu
5.648LeuAla: 5.648 ± 0.563
1.007LeuCys: 1.007 ± 0.23
4.028LeuAsp: 4.028 ± 0.376
5.56LeuGlu: 5.56 ± 0.616
2.89LeuPhe: 2.89 ± 0.415
4.991LeuGly: 4.991 ± 0.618
1.095LeuHis: 1.095 ± 0.284
3.765LeuIle: 3.765 ± 0.605
4.466LeuLys: 4.466 ± 0.428
4.86LeuLeu: 4.86 ± 0.522
2.145LeuMet: 2.145 ± 0.284
4.115LeuAsn: 4.115 ± 0.454
3.065LeuPro: 3.065 ± 0.34
3.327LeuGln: 3.327 ± 0.484
4.334LeuArg: 4.334 ± 0.408
5.91LeuSer: 5.91 ± 0.589
4.86LeuThr: 4.86 ± 0.549
4.86LeuVal: 4.86 ± 0.437
0.7LeuTrp: 0.7 ± 0.191
2.277LeuTyr: 2.277 ± 0.264
0.0LeuXaa: 0.0 ± 0.0
Met
2.189MetAla: 2.189 ± 0.344
0.263MetCys: 0.263 ± 0.15
1.97MetAsp: 1.97 ± 0.291
1.839MetGlu: 1.839 ± 0.308
1.226MetPhe: 1.226 ± 0.241
1.532MetGly: 1.532 ± 0.268
0.306MetHis: 0.306 ± 0.113
1.489MetIle: 1.489 ± 0.279
2.277MetLys: 2.277 ± 0.384
2.32MetLeu: 2.32 ± 0.381
0.744MetMet: 0.744 ± 0.185
1.707MetAsn: 1.707 ± 0.262
0.876MetPro: 0.876 ± 0.177
0.963MetGln: 0.963 ± 0.208
1.489MetArg: 1.489 ± 0.266
2.277MetSer: 2.277 ± 0.32
1.795MetThr: 1.795 ± 0.242
1.401MetVal: 1.401 ± 0.307
0.263MetTrp: 0.263 ± 0.113
0.7MetTyr: 0.7 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
4.991AsnAla: 4.991 ± 0.499
0.306AsnCys: 0.306 ± 0.1
2.846AsnAsp: 2.846 ± 0.306
2.627AsnGlu: 2.627 ± 0.36
2.408AsnPhe: 2.408 ± 0.371
4.334AsnGly: 4.334 ± 0.506
0.744AsnHis: 0.744 ± 0.206
3.196AsnIle: 3.196 ± 0.354
3.459AsnLys: 3.459 ± 0.368
4.028AsnLeu: 4.028 ± 0.485
1.182AsnMet: 1.182 ± 0.237
2.846AsnAsn: 2.846 ± 0.394
3.021AsnPro: 3.021 ± 0.366
2.758AsnGln: 2.758 ± 0.445
3.065AsnArg: 3.065 ± 0.391
3.94AsnSer: 3.94 ± 0.379
3.459AsnThr: 3.459 ± 0.448
3.415AsnVal: 3.415 ± 0.321
0.876AsnTrp: 0.876 ± 0.174
1.883AsnTyr: 1.883 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
2.802ProAla: 2.802 ± 0.297
0.35ProCys: 0.35 ± 0.141
2.933ProAsp: 2.933 ± 0.397
3.065ProGlu: 3.065 ± 0.469
1.664ProPhe: 1.664 ± 0.24
1.576ProGly: 1.576 ± 0.266
0.7ProHis: 0.7 ± 0.194
2.145ProIle: 2.145 ± 0.257
2.496ProLys: 2.496 ± 0.372
2.714ProLeu: 2.714 ± 0.422
0.832ProMet: 0.832 ± 0.206
1.839ProAsn: 1.839 ± 0.275
1.27ProPro: 1.27 ± 0.224
1.62ProGln: 1.62 ± 0.23
1.226ProArg: 1.226 ± 0.275
2.846ProSer: 2.846 ± 0.356
2.233ProThr: 2.233 ± 0.343
3.284ProVal: 3.284 ± 0.379
0.35ProTrp: 0.35 ± 0.119
0.919ProTyr: 0.919 ± 0.217
0.0ProXaa: 0.0 ± 0.0
Gln
4.159GlnAla: 4.159 ± 0.759
0.876GlnCys: 0.876 ± 0.236
2.058GlnAsp: 2.058 ± 0.348
2.758GlnGlu: 2.758 ± 0.42
1.313GlnPhe: 1.313 ± 0.207
2.539GlnGly: 2.539 ± 0.365
0.438GlnHis: 0.438 ± 0.148
2.32GlnIle: 2.32 ± 0.357
2.364GlnLys: 2.364 ± 0.306
2.671GlnLeu: 2.671 ± 0.401
1.095GlnMet: 1.095 ± 0.194
2.627GlnAsn: 2.627 ± 0.452
1.095GlnPro: 1.095 ± 0.202
2.452GlnGln: 2.452 ± 0.592
2.408GlnArg: 2.408 ± 0.348
2.277GlnSer: 2.277 ± 0.314
2.233GlnThr: 2.233 ± 0.39
3.152GlnVal: 3.152 ± 0.401
0.569GlnTrp: 0.569 ± 0.159
1.532GlnTyr: 1.532 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
3.853ArgAla: 3.853 ± 0.672
0.613ArgCys: 0.613 ± 0.159
3.327ArgAsp: 3.327 ± 0.37
3.809ArgGlu: 3.809 ± 0.539
1.926ArgPhe: 1.926 ± 0.26
2.758ArgGly: 2.758 ± 0.334
1.007ArgHis: 1.007 ± 0.232
3.327ArgIle: 3.327 ± 0.383
2.802ArgLys: 2.802 ± 0.437
3.897ArgLeu: 3.897 ± 0.377
1.27ArgMet: 1.27 ± 0.214
2.758ArgAsn: 2.758 ± 0.363
1.751ArgPro: 1.751 ± 0.272
2.583ArgGln: 2.583 ± 0.381
3.196ArgArg: 3.196 ± 0.465
3.502ArgSer: 3.502 ± 0.427
2.627ArgThr: 2.627 ± 0.385
4.334ArgVal: 4.334 ± 0.42
0.919ArgTrp: 0.919 ± 0.176
1.576ArgTyr: 1.576 ± 0.233
0.0ArgXaa: 0.0 ± 0.0
Ser
4.466SerAla: 4.466 ± 0.553
0.657SerCys: 0.657 ± 0.177
4.115SerAsp: 4.115 ± 0.451
3.853SerGlu: 3.853 ± 0.436
2.802SerPhe: 2.802 ± 0.356
6.261SerGly: 6.261 ± 0.683
1.357SerHis: 1.357 ± 0.301
3.284SerIle: 3.284 ± 0.349
4.422SerLys: 4.422 ± 0.562
5.56SerLeu: 5.56 ± 0.475
2.101SerMet: 2.101 ± 0.382
4.028SerAsn: 4.028 ± 0.503
3.108SerPro: 3.108 ± 0.511
2.189SerGln: 2.189 ± 0.376
3.284SerArg: 3.284 ± 0.384
4.641SerSer: 4.641 ± 0.493
4.159SerThr: 4.159 ± 0.493
4.991SerVal: 4.991 ± 0.511
1.182SerTrp: 1.182 ± 0.211
2.802SerTyr: 2.802 ± 0.337
0.0SerXaa: 0.0 ± 0.0
Thr
4.422ThrAla: 4.422 ± 0.662
0.438ThrCys: 0.438 ± 0.169
3.809ThrAsp: 3.809 ± 0.398
3.634ThrGlu: 3.634 ± 0.377
2.277ThrPhe: 2.277 ± 0.311
5.297ThrGly: 5.297 ± 0.614
1.051ThrHis: 1.051 ± 0.208
3.327ThrIle: 3.327 ± 0.533
3.502ThrLys: 3.502 ± 0.422
4.291ThrLeu: 4.291 ± 0.414
0.744ThrMet: 0.744 ± 0.156
2.89ThrAsn: 2.89 ± 0.425
2.933ThrPro: 2.933 ± 0.287
2.101ThrGln: 2.101 ± 0.29
2.496ThrArg: 2.496 ± 0.286
3.634ThrSer: 3.634 ± 0.432
3.94ThrThr: 3.94 ± 0.545
4.685ThrVal: 4.685 ± 0.557
0.919ThrTrp: 0.919 ± 0.303
1.707ThrTyr: 1.707 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
5.254ValAla: 5.254 ± 0.448
1.226ValCys: 1.226 ± 0.273
4.072ValAsp: 4.072 ± 0.616
5.122ValGlu: 5.122 ± 0.499
2.714ValPhe: 2.714 ± 0.436
5.079ValGly: 5.079 ± 0.424
1.445ValHis: 1.445 ± 0.276
3.897ValIle: 3.897 ± 0.475
4.86ValLys: 4.86 ± 0.466
4.728ValLeu: 4.728 ± 0.458
1.62ValMet: 1.62 ± 0.299
4.334ValAsn: 4.334 ± 0.519
2.101ValPro: 2.101 ± 0.341
2.408ValGln: 2.408 ± 0.361
3.021ValArg: 3.021 ± 0.345
4.903ValSer: 4.903 ± 0.535
4.728ValThr: 4.728 ± 0.604
5.385ValVal: 5.385 ± 0.551
1.445ValTrp: 1.445 ± 0.334
2.496ValTyr: 2.496 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
0.876TrpAla: 0.876 ± 0.197
0.219TrpCys: 0.219 ± 0.117
1.138TrpAsp: 1.138 ± 0.217
0.744TrpGlu: 0.744 ± 0.163
0.657TrpPhe: 0.657 ± 0.152
1.138TrpGly: 1.138 ± 0.282
0.306TrpHis: 0.306 ± 0.121
1.007TrpIle: 1.007 ± 0.215
0.876TrpLys: 0.876 ± 0.217
0.7TrpLeu: 0.7 ± 0.176
0.7TrpMet: 0.7 ± 0.194
0.919TrpAsn: 0.919 ± 0.193
0.438TrpPro: 0.438 ± 0.138
0.438TrpGln: 0.438 ± 0.162
0.876TrpArg: 0.876 ± 0.159
0.919TrpSer: 0.919 ± 0.17
1.051TrpThr: 1.051 ± 0.185
1.095TrpVal: 1.095 ± 0.191
0.088TrpTrp: 0.088 ± 0.081
0.482TrpTyr: 0.482 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.364TyrAla: 2.364 ± 0.266
0.306TyrCys: 0.306 ± 0.113
2.145TyrAsp: 2.145 ± 0.328
2.583TyrGlu: 2.583 ± 0.307
1.007TyrPhe: 1.007 ± 0.222
2.452TyrGly: 2.452 ± 0.308
0.876TyrHis: 0.876 ± 0.218
1.751TyrIle: 1.751 ± 0.294
1.795TyrLys: 1.795 ± 0.245
2.233TyrLeu: 2.233 ± 0.277
1.138TyrMet: 1.138 ± 0.171
1.751TyrAsn: 1.751 ± 0.229
1.576TyrPro: 1.576 ± 0.276
1.839TyrGln: 1.839 ± 0.226
2.014TyrArg: 2.014 ± 0.367
2.539TyrSer: 2.539 ± 0.343
1.97TyrThr: 1.97 ± 0.28
2.058TyrVal: 2.058 ± 0.299
0.306TyrTrp: 0.306 ± 0.09
1.226TyrTyr: 1.226 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 125 proteins (22842 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski