Edaphobacter sp. 12200R-103

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Edaphobacter; unclassified Edaphobacter

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9YNG7|A0A6B9YNG7_9BACT Flagellar basal body rod protein FlgB OS=Edaphobacter sp. 12200R-103 OX=2703788 GN=flgB PE=3 SV=1
MM1 pKa = 8.06AIQADD6 pKa = 4.14FNGDD10 pKa = 3.07GRR12 pKa = 11.84PDD14 pKa = 3.62FFYY17 pKa = 11.04YY18 pKa = 10.65DD19 pKa = 3.39PLYY22 pKa = 10.75RR23 pKa = 11.84PNTSIRR29 pKa = 11.84TEE31 pKa = 3.8ILLSHH36 pKa = 7.2ADD38 pKa = 3.5GSYY41 pKa = 10.79SDD43 pKa = 4.61PLAVSLDD50 pKa = 3.68GTPQQCHH57 pKa = 6.6PYY59 pKa = 9.97DD60 pKa = 3.48INGDD64 pKa = 3.6HH65 pKa = 7.0FADD68 pKa = 4.42LVCISFASGSPALQVMYY85 pKa = 10.81SFIGNGDD92 pKa = 3.52GTFQPSIRR100 pKa = 11.84TDD102 pKa = 2.8ISINNDD108 pKa = 3.02FYY110 pKa = 11.79SSVGVTIAGDD120 pKa = 3.37INGDD124 pKa = 3.4GKK126 pKa = 11.37LDD128 pKa = 3.92VILTNLDD135 pKa = 3.79RR136 pKa = 11.84LNTIYY141 pKa = 10.77PMLGDD146 pKa = 3.8GAGHH150 pKa = 5.87FTLGKK155 pKa = 9.09PLNLRR160 pKa = 11.84YY161 pKa = 9.73YY162 pKa = 9.65SSGVRR167 pKa = 11.84LYY169 pKa = 11.25DD170 pKa = 3.48EE171 pKa = 4.57LHH173 pKa = 6.85DD174 pKa = 4.12VNGDD178 pKa = 3.44GKK180 pKa = 11.32LDD182 pKa = 3.94LLYY185 pKa = 10.62GQPLQVYY192 pKa = 9.14LGNGDD197 pKa = 3.68GTFTSGFIAGNYY209 pKa = 6.55QNCFFTDD216 pKa = 4.27FDD218 pKa = 4.27NDD220 pKa = 3.17QHH222 pKa = 8.41LDD224 pKa = 3.74AEE226 pKa = 4.83CVDD229 pKa = 3.58ISSNSNIILHH239 pKa = 6.31GNPDD243 pKa = 3.37GTFNTTPIATPDD255 pKa = 3.74FSNTTVLAIKK265 pKa = 10.03DD266 pKa = 3.92LNGDD270 pKa = 3.93GMPDD274 pKa = 3.96VISLGPKK281 pKa = 8.52GTIIYY286 pKa = 9.79LGKK289 pKa = 9.69PGLKK293 pKa = 9.34FAPPIAYY300 pKa = 9.72SFSAGYY306 pKa = 10.98SNFGVTDD313 pKa = 4.05PLIDD317 pKa = 5.13DD318 pKa = 4.11YY319 pKa = 11.97NGDD322 pKa = 4.35GILDD326 pKa = 4.39LATTAFDD333 pKa = 5.19GIYY336 pKa = 9.64IAYY339 pKa = 9.69GRR341 pKa = 11.84GDD343 pKa = 3.43GSFRR347 pKa = 11.84APRR350 pKa = 11.84PTEE353 pKa = 3.82SGTAIGGIAVGDD365 pKa = 3.94FDD367 pKa = 5.91EE368 pKa = 6.47DD369 pKa = 3.82GAPDD373 pKa = 4.44VITSGSPSLLLNHH386 pKa = 7.01GKK388 pKa = 10.83GDD390 pKa = 3.76GTFATSTPIQRR401 pKa = 11.84DD402 pKa = 3.26GLAANAGISSQVFQGDD418 pKa = 3.91FNGDD422 pKa = 2.97GHH424 pKa = 7.98LDD426 pKa = 3.41LVTNNSGSLAQIFFGKK442 pKa = 10.7GDD444 pKa = 3.75GTFSDD449 pKa = 4.53PVFASSTFNFIPFQGAVVADD469 pKa = 3.53INGDD473 pKa = 3.21GRR475 pKa = 11.84ADD477 pKa = 4.88LIAPNNSYY485 pKa = 8.33PTGDD489 pKa = 3.19VWVMLSNGDD498 pKa = 3.53GSFSAQSIATFNSALSVAAGDD519 pKa = 3.76VNGDD523 pKa = 3.34NKK525 pKa = 10.93LDD527 pKa = 3.89LVVSIAGGVQIFLGKK542 pKa = 10.66GDD544 pKa = 3.6GTFNQVVSAPAAPSTGNGTFSPGQAVFGDD573 pKa = 3.84FDD575 pKa = 5.49GDD577 pKa = 3.72GKK579 pKa = 11.07RR580 pKa = 11.84DD581 pKa = 3.57FVVTNNLSTYY591 pKa = 10.45YY592 pKa = 9.86LTIYY596 pKa = 9.65YY597 pKa = 10.61GNGDD601 pKa = 3.9GTFSAPSTIATGVSPTGLFLNLAAHH626 pKa = 7.02DD627 pKa = 4.54LNGDD631 pKa = 3.75GLDD634 pKa = 3.78DD635 pKa = 4.34LLYY638 pKa = 10.31SGSSIYY644 pKa = 10.21QVAEE648 pKa = 3.5ISILHH653 pKa = 6.51AKK655 pKa = 9.41PGRR658 pKa = 11.84TFDD661 pKa = 3.92AGMGLLAGFGTLTPSIADD679 pKa = 3.58FNKK682 pKa = 10.44DD683 pKa = 3.23GRR685 pKa = 11.84PDD687 pKa = 3.67LLFSNSYY694 pKa = 10.17ASPGIFTVLLNRR706 pKa = 11.84EE707 pKa = 4.39STGTTLTVDD716 pKa = 3.66HH717 pKa = 6.64TAIQYY722 pKa = 8.68GQGAGFTAQVTFSADD737 pKa = 3.31STSAPPPSATVILSGLPGSPVTLPIAFSSPGANAPFSGTARR778 pKa = 11.84YY779 pKa = 8.04EE780 pKa = 4.46AINLLPGTYY789 pKa = 10.29NVTARR794 pKa = 11.84LNASCCLTPSKK805 pKa = 10.81SAVTTLVVAPAATTTTLALTPVPPLTGQPATLRR838 pKa = 11.84VQVSSQTTVTSGTITFFDD856 pKa = 3.29QGTQIGTAPVASGAASFTTSPLSAGAHH883 pKa = 6.32SITAVFASDD892 pKa = 3.62QNFLTSSSQPLTVTVFDD909 pKa = 4.68RR910 pKa = 11.84GYY912 pKa = 10.79QLTAAQTNLSLGANGSTDD930 pKa = 4.33LVTLSPVQGFYY941 pKa = 11.49GKK943 pKa = 8.39VTLSWLGHH951 pKa = 6.24AGG953 pKa = 3.34

Molecular weight: 98.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9YS72|A0A6B9YS72_9BACT Patatin-like phospholipase family protein OS=Edaphobacter sp. 12200R-103 OX=2703788 GN=GWR55_12050 PE=4 SV=1
MM1 pKa = 6.98EE2 pKa = 4.71TVSLADD8 pKa = 2.92IDD10 pKa = 4.02KK11 pKa = 10.9FVVAKK16 pKa = 10.17RR17 pKa = 11.84AEE19 pKa = 4.17GCRR22 pKa = 11.84PRR24 pKa = 11.84GIANQCKK31 pKa = 9.72ALRR34 pKa = 11.84SFFRR38 pKa = 11.84FAEE41 pKa = 3.99MRR43 pKa = 11.84GWCVPNLALTIRR55 pKa = 11.84IPRR58 pKa = 11.84IPKK61 pKa = 9.71NEE63 pKa = 3.88PRR65 pKa = 11.84PTGPTWTQVRR75 pKa = 11.84QLLEE79 pKa = 4.03LTEE82 pKa = 4.82GNDD85 pKa = 3.57PEE87 pKa = 4.91HH88 pKa = 6.37IRR90 pKa = 11.84ARR92 pKa = 11.84ALLLLFTIYY101 pKa = 10.9GLRR104 pKa = 11.84ASEE107 pKa = 4.35VINLRR112 pKa = 11.84LEE114 pKa = 4.43DD115 pKa = 4.64FDD117 pKa = 3.76WQSEE121 pKa = 4.61VFTIRR126 pKa = 11.84RR127 pKa = 11.84AKK129 pKa = 10.56HH130 pKa = 6.17GGTQQYY136 pKa = 9.05PIQYY140 pKa = 8.96EE141 pKa = 3.98VGEE144 pKa = 4.99AILKK148 pKa = 7.24YY149 pKa = 10.27LRR151 pKa = 11.84YY152 pKa = 9.59VRR154 pKa = 11.84PRR156 pKa = 11.84VDD158 pKa = 3.33DD159 pKa = 3.48RR160 pKa = 11.84HH161 pKa = 6.19VFLGEE166 pKa = 3.8RR167 pKa = 11.84RR168 pKa = 11.84PWGPLQHH175 pKa = 4.97TTMWRR180 pKa = 11.84TVSRR184 pKa = 11.84RR185 pKa = 11.84LGALGIEE192 pKa = 4.67LRR194 pKa = 11.84HH195 pKa = 5.8QGPHH199 pKa = 6.56ALRR202 pKa = 11.84HH203 pKa = 5.05ACATRR208 pKa = 11.84LLQKK212 pKa = 10.65GSSLKK217 pKa = 10.59EE218 pKa = 3.43IADD221 pKa = 3.9FLGHH225 pKa = 7.08RR226 pKa = 11.84NTKK229 pKa = 10.29SVGIYY234 pKa = 9.85AKK236 pKa = 10.47CDD238 pKa = 2.83IAALRR243 pKa = 11.84KK244 pKa = 9.31VAAFRR249 pKa = 11.84LGGLRR254 pKa = 3.77

Molecular weight: 29.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3688

0

3688

1316478

24

2344

357.0

39.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.495 ± 0.05

0.851 ± 0.015

5.07 ± 0.024

5.504 ± 0.05

3.913 ± 0.026

8.031 ± 0.04

2.317 ± 0.019

5.256 ± 0.026

3.743 ± 0.034

9.802 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.319 ± 0.022

3.457 ± 0.044

5.332 ± 0.03

3.827 ± 0.026

6.339 ± 0.039

6.471 ± 0.036

5.962 ± 0.043

7.134 ± 0.032

1.383 ± 0.015

2.794 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski