Amino acid dipepetide frequency for Achromobacter phage JWAlpha

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.321AlaAla: 11.321 ± 1.322
0.479AlaCys: 0.479 ± 0.179
4.964AlaAsp: 4.964 ± 0.516
6.488AlaGlu: 6.488 ± 0.87
2.569AlaPhe: 2.569 ± 0.381
6.357AlaGly: 6.357 ± 0.882
1.742AlaHis: 1.742 ± 0.358
4.659AlaIle: 4.659 ± 0.351
5.225AlaLys: 5.225 ± 0.614
9.187AlaLeu: 9.187 ± 1.405
3.092AlaMet: 3.092 ± 0.341
4.703AlaAsn: 4.703 ± 0.441
4.485AlaPro: 4.485 ± 0.618
4.616AlaGln: 4.616 ± 0.64
4.093AlaArg: 4.093 ± 0.45
4.528AlaSer: 4.528 ± 0.463
5.53AlaThr: 5.53 ± 0.654
6.618AlaVal: 6.618 ± 0.765
1.219AlaTrp: 1.219 ± 0.209
3.353AlaTyr: 3.353 ± 0.362
0.0AlaXaa: 0.0 ± 0.0
Cys
0.566CysAla: 0.566 ± 0.19
0.174CysCys: 0.174 ± 0.099
0.479CysAsp: 0.479 ± 0.152
0.479CysGlu: 0.479 ± 0.199
0.392CysPhe: 0.392 ± 0.138
1.001CysGly: 1.001 ± 0.285
0.261CysHis: 0.261 ± 0.13
0.566CysIle: 0.566 ± 0.183
0.784CysLys: 0.784 ± 0.219
0.74CysLeu: 0.74 ± 0.235
0.305CysMet: 0.305 ± 0.118
0.479CysAsn: 0.479 ± 0.177
0.566CysPro: 0.566 ± 0.197
0.174CysGln: 0.174 ± 0.085
0.392CysArg: 0.392 ± 0.145
0.305CysSer: 0.305 ± 0.14
0.566CysThr: 0.566 ± 0.174
0.566CysVal: 0.566 ± 0.179
0.261CysTrp: 0.261 ± 0.108
0.131CysTyr: 0.131 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
6.618AspAla: 6.618 ± 0.73
0.61AspCys: 0.61 ± 0.187
2.961AspAsp: 2.961 ± 0.408
4.006AspGlu: 4.006 ± 0.434
2.438AspPhe: 2.438 ± 0.301
4.311AspGly: 4.311 ± 0.529
1.655AspHis: 1.655 ± 0.246
2.917AspIle: 2.917 ± 0.416
2.874AspLys: 2.874 ± 0.351
5.486AspLeu: 5.486 ± 0.467
2.047AspMet: 2.047 ± 0.264
2.264AspAsn: 2.264 ± 0.337
3.483AspPro: 3.483 ± 0.382
2.743AspGln: 2.743 ± 0.287
3.658AspArg: 3.658 ± 0.372
2.83AspSer: 2.83 ± 0.324
3.004AspThr: 3.004 ± 0.379
3.832AspVal: 3.832 ± 0.365
0.871AspTrp: 0.871 ± 0.216
1.829AspTyr: 1.829 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
6.618GluAla: 6.618 ± 0.943
0.435GluCys: 0.435 ± 0.158
3.266GluAsp: 3.266 ± 0.374
4.616GluGlu: 4.616 ± 0.575
1.785GluPhe: 1.785 ± 0.286
4.093GluGly: 4.093 ± 0.494
1.132GluHis: 1.132 ± 0.247
3.353GluIle: 3.353 ± 0.53
3.309GluLys: 3.309 ± 0.414
5.182GluLeu: 5.182 ± 0.614
2.09GluMet: 2.09 ± 0.252
2.308GluAsn: 2.308 ± 0.318
1.829GluPro: 1.829 ± 0.363
2.787GluGln: 2.787 ± 0.315
2.874GluArg: 2.874 ± 0.332
3.309GluSer: 3.309 ± 0.348
2.874GluThr: 2.874 ± 0.324
4.616GluVal: 4.616 ± 0.607
0.871GluTrp: 0.871 ± 0.159
2.047GluTyr: 2.047 ± 0.282
0.0GluXaa: 0.0 ± 0.0
Phe
2.351PheAla: 2.351 ± 0.377
0.305PheCys: 0.305 ± 0.131
3.004PheAsp: 3.004 ± 0.352
1.001PheGlu: 1.001 ± 0.182
1.524PhePhe: 1.524 ± 0.206
3.048PheGly: 3.048 ± 0.353
0.61PheHis: 0.61 ± 0.203
2.047PheIle: 2.047 ± 0.255
1.916PheLys: 1.916 ± 0.36
2.787PheLeu: 2.787 ± 0.325
1.524PheMet: 1.524 ± 0.267
1.742PheAsn: 1.742 ± 0.316
1.219PhePro: 1.219 ± 0.27
1.219PheGln: 1.219 ± 0.225
2.177PheArg: 2.177 ± 0.323
2.395PheSer: 2.395 ± 0.312
2.047PheThr: 2.047 ± 0.287
1.959PheVal: 1.959 ± 0.247
0.479PheTrp: 0.479 ± 0.122
1.132PheTyr: 1.132 ± 0.208
0.0PheXaa: 0.0 ± 0.0
Gly
5.443GlyAla: 5.443 ± 0.651
0.74GlyCys: 0.74 ± 0.197
4.049GlyAsp: 4.049 ± 0.494
3.875GlyGlu: 3.875 ± 0.373
2.917GlyPhe: 2.917 ± 0.31
5.53GlyGly: 5.53 ± 0.832
1.045GlyHis: 1.045 ± 0.221
4.137GlyIle: 4.137 ± 0.45
4.833GlyLys: 4.833 ± 0.44
5.312GlyLeu: 5.312 ± 0.631
2.874GlyMet: 2.874 ± 0.359
4.18GlyAsn: 4.18 ± 0.465
2.134GlyPro: 2.134 ± 0.316
2.961GlyGln: 2.961 ± 0.435
3.658GlyArg: 3.658 ± 0.433
5.007GlySer: 5.007 ± 0.493
4.616GlyThr: 4.616 ± 0.461
5.443GlyVal: 5.443 ± 0.495
1.176GlyTrp: 1.176 ± 0.265
2.482GlyTyr: 2.482 ± 0.294
0.0GlyXaa: 0.0 ± 0.0
His
1.872HisAla: 1.872 ± 0.279
0.392HisCys: 0.392 ± 0.15
1.393HisAsp: 1.393 ± 0.253
0.914HisGlu: 0.914 ± 0.178
0.871HisPhe: 0.871 ± 0.241
1.132HisGly: 1.132 ± 0.292
0.479HisHis: 0.479 ± 0.199
1.001HisIle: 1.001 ± 0.309
1.568HisLys: 1.568 ± 0.263
2.177HisLeu: 2.177 ± 0.361
0.61HisMet: 0.61 ± 0.137
1.132HisAsn: 1.132 ± 0.195
1.437HisPro: 1.437 ± 0.327
0.697HisGln: 0.697 ± 0.166
1.698HisArg: 1.698 ± 0.342
0.958HisSer: 0.958 ± 0.209
0.827HisThr: 0.827 ± 0.2
1.437HisVal: 1.437 ± 0.309
0.392HisTrp: 0.392 ± 0.124
0.827HisTyr: 0.827 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
4.572IleAla: 4.572 ± 0.535
0.305IleCys: 0.305 ± 0.125
3.962IleAsp: 3.962 ± 0.424
2.917IleGlu: 2.917 ± 0.414
1.524IlePhe: 1.524 ± 0.249
3.092IleGly: 3.092 ± 0.344
1.393IleHis: 1.393 ± 0.211
2.482IleIle: 2.482 ± 0.492
3.222IleLys: 3.222 ± 0.359
3.658IleLeu: 3.658 ± 0.459
1.393IleMet: 1.393 ± 0.214
2.177IleAsn: 2.177 ± 0.35
2.917IlePro: 2.917 ± 0.43
2.569IleGln: 2.569 ± 0.328
2.7IleArg: 2.7 ± 0.335
2.7IleSer: 2.7 ± 0.316
4.049IleThr: 4.049 ± 0.425
3.179IleVal: 3.179 ± 0.455
0.74IleTrp: 0.74 ± 0.208
1.524IleTyr: 1.524 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
5.225LysAla: 5.225 ± 0.542
0.261LysCys: 0.261 ± 0.12
3.701LysAsp: 3.701 ± 0.404
3.745LysGlu: 3.745 ± 0.475
2.09LysPhe: 2.09 ± 0.27
3.658LysGly: 3.658 ± 0.368
1.306LysHis: 1.306 ± 0.277
2.09LysIle: 2.09 ± 0.248
3.048LysLys: 3.048 ± 0.394
5.312LysLeu: 5.312 ± 0.575
2.134LysMet: 2.134 ± 0.351
2.482LysAsn: 2.482 ± 0.272
3.004LysPro: 3.004 ± 0.454
3.44LysGln: 3.44 ± 0.352
3.048LysArg: 3.048 ± 0.45
2.787LysSer: 2.787 ± 0.446
4.703LysThr: 4.703 ± 0.461
3.483LysVal: 3.483 ± 0.42
0.74LysTrp: 0.74 ± 0.173
1.437LysTyr: 1.437 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
8.012LeuAla: 8.012 ± 0.893
0.784LeuCys: 0.784 ± 0.242
6.14LeuAsp: 6.14 ± 0.506
4.528LeuGlu: 4.528 ± 0.389
3.004LeuPhe: 3.004 ± 0.333
6.27LeuGly: 6.27 ± 0.588
2.264LeuHis: 2.264 ± 0.341
3.179LeuIle: 3.179 ± 0.275
4.92LeuLys: 4.92 ± 0.398
6.27LeuLeu: 6.27 ± 0.643
2.613LeuMet: 2.613 ± 0.315
4.398LeuAsn: 4.398 ± 0.393
3.832LeuPro: 3.832 ± 0.335
3.614LeuGln: 3.614 ± 0.441
4.224LeuArg: 4.224 ± 0.464
4.528LeuSer: 4.528 ± 0.506
5.138LeuThr: 5.138 ± 0.458
5.965LeuVal: 5.965 ± 0.533
0.653LeuTrp: 0.653 ± 0.164
2.221LeuTyr: 2.221 ± 0.295
0.0LeuXaa: 0.0 ± 0.0
Met
3.309MetAla: 3.309 ± 0.394
0.305MetCys: 0.305 ± 0.138
1.698MetAsp: 1.698 ± 0.266
1.611MetGlu: 1.611 ± 0.244
0.74MetPhe: 0.74 ± 0.181
2.047MetGly: 2.047 ± 0.279
0.566MetHis: 0.566 ± 0.179
1.959MetIle: 1.959 ± 0.314
2.308MetLys: 2.308 ± 0.324
2.525MetLeu: 2.525 ± 0.37
0.827MetMet: 0.827 ± 0.234
1.698MetAsn: 1.698 ± 0.292
1.568MetPro: 1.568 ± 0.246
2.003MetGln: 2.003 ± 0.236
1.263MetArg: 1.263 ± 0.215
3.048MetSer: 3.048 ± 0.344
2.613MetThr: 2.613 ± 0.356
1.829MetVal: 1.829 ± 0.366
0.261MetTrp: 0.261 ± 0.126
0.871MetTyr: 0.871 ± 0.2
0.0MetXaa: 0.0 ± 0.0
Asn
3.483AsnAla: 3.483 ± 0.364
0.392AsnCys: 0.392 ± 0.151
2.264AsnAsp: 2.264 ± 0.244
2.482AsnGlu: 2.482 ± 0.305
1.393AsnPhe: 1.393 ± 0.229
3.309AsnGly: 3.309 ± 0.371
1.263AsnHis: 1.263 ± 0.23
2.221AsnIle: 2.221 ± 0.479
2.569AsnLys: 2.569 ± 0.373
4.093AsnLeu: 4.093 ± 0.437
1.176AsnMet: 1.176 ± 0.235
2.961AsnAsn: 2.961 ± 0.6
3.092AsnPro: 3.092 ± 0.312
3.004AsnGln: 3.004 ± 0.324
2.308AsnArg: 2.308 ± 0.266
3.048AsnSer: 3.048 ± 0.366
2.917AsnThr: 2.917 ± 0.403
2.83AsnVal: 2.83 ± 0.416
0.784AsnTrp: 0.784 ± 0.228
1.524AsnTyr: 1.524 ± 0.259
0.0AsnXaa: 0.0 ± 0.0
Pro
4.485ProAla: 4.485 ± 0.527
0.566ProCys: 0.566 ± 0.161
2.395ProAsp: 2.395 ± 0.439
3.527ProGlu: 3.527 ± 0.508
2.003ProPhe: 2.003 ± 0.357
3.57ProGly: 3.57 ± 0.443
0.479ProHis: 0.479 ± 0.151
2.09ProIle: 2.09 ± 0.324
2.743ProLys: 2.743 ± 0.36
3.048ProLeu: 3.048 ± 0.351
1.263ProMet: 1.263 ± 0.205
1.306ProAsn: 1.306 ± 0.254
1.698ProPro: 1.698 ± 0.374
1.698ProGln: 1.698 ± 0.325
1.655ProArg: 1.655 ± 0.322
2.743ProSer: 2.743 ± 0.276
3.919ProThr: 3.919 ± 0.327
4.311ProVal: 4.311 ± 0.565
0.566ProTrp: 0.566 ± 0.148
0.958ProTyr: 0.958 ± 0.221
0.0ProXaa: 0.0 ± 0.0
Gln
6.14GlnAla: 6.14 ± 0.888
0.261GlnCys: 0.261 ± 0.115
2.395GlnAsp: 2.395 ± 0.373
2.83GlnGlu: 2.83 ± 0.492
1.698GlnPhe: 1.698 ± 0.265
3.919GlnGly: 3.919 ± 0.456
0.914GlnHis: 0.914 ± 0.215
2.7GlnIle: 2.7 ± 0.327
2.308GlnLys: 2.308 ± 0.34
3.527GlnLeu: 3.527 ± 0.274
1.742GlnMet: 1.742 ± 0.254
2.134GlnAsn: 2.134 ± 0.369
1.35GlnPro: 1.35 ± 0.239
3.048GlnGln: 3.048 ± 0.404
2.613GlnArg: 2.613 ± 0.367
3.179GlnSer: 3.179 ± 0.559
2.003GlnThr: 2.003 ± 0.242
2.7GlnVal: 2.7 ± 0.337
0.74GlnTrp: 0.74 ± 0.151
1.742GlnTyr: 1.742 ± 0.367
0.0GlnXaa: 0.0 ± 0.0
Arg
4.659ArgAla: 4.659 ± 0.528
0.74ArgCys: 0.74 ± 0.239
3.396ArgAsp: 3.396 ± 0.402
3.179ArgGlu: 3.179 ± 0.462
1.959ArgPhe: 1.959 ± 0.291
3.179ArgGly: 3.179 ± 0.404
0.914ArgHis: 0.914 ± 0.264
3.222ArgIle: 3.222 ± 0.451
2.83ArgLys: 2.83 ± 0.395
5.007ArgLeu: 5.007 ± 0.377
2.09ArgMet: 2.09 ± 0.297
1.742ArgAsn: 1.742 ± 0.231
2.003ArgPro: 2.003 ± 0.281
2.613ArgGln: 2.613 ± 0.466
1.916ArgArg: 1.916 ± 0.406
3.527ArgSer: 3.527 ± 0.413
2.525ArgThr: 2.525 ± 0.339
3.092ArgVal: 3.092 ± 0.318
0.74ArgTrp: 0.74 ± 0.231
1.48ArgTyr: 1.48 ± 0.248
0.0ArgXaa: 0.0 ± 0.0
Ser
5.138SerAla: 5.138 ± 0.492
0.566SerCys: 0.566 ± 0.185
3.004SerAsp: 3.004 ± 0.372
3.092SerGlu: 3.092 ± 0.387
1.872SerPhe: 1.872 ± 0.352
4.528SerGly: 4.528 ± 0.516
1.263SerHis: 1.263 ± 0.274
3.135SerIle: 3.135 ± 0.381
3.483SerLys: 3.483 ± 0.321
5.443SerLeu: 5.443 ± 0.423
1.742SerMet: 1.742 ± 0.305
3.396SerAsn: 3.396 ± 0.384
2.351SerPro: 2.351 ± 0.286
2.438SerGln: 2.438 ± 0.409
3.57SerArg: 3.57 ± 0.397
3.44SerSer: 3.44 ± 0.419
4.049SerThr: 4.049 ± 0.522
4.311SerVal: 4.311 ± 0.517
0.479SerTrp: 0.479 ± 0.144
1.785SerTyr: 1.785 ± 0.301
0.0SerXaa: 0.0 ± 0.0
Thr
6.052ThrAla: 6.052 ± 0.581
0.61ThrCys: 0.61 ± 0.24
4.049ThrAsp: 4.049 ± 0.365
3.614ThrGlu: 3.614 ± 0.35
2.264ThrPhe: 2.264 ± 0.274
4.877ThrGly: 4.877 ± 0.478
1.045ThrHis: 1.045 ± 0.299
3.135ThrIle: 3.135 ± 0.398
3.004ThrLys: 3.004 ± 0.349
4.267ThrLeu: 4.267 ± 0.338
1.916ThrMet: 1.916 ± 0.264
2.525ThrAsn: 2.525 ± 0.41
3.135ThrPro: 3.135 ± 0.498
2.351ThrGln: 2.351 ± 0.319
3.179ThrArg: 3.179 ± 0.325
3.483ThrSer: 3.483 ± 0.329
4.18ThrThr: 4.18 ± 0.63
4.485ThrVal: 4.485 ± 0.592
0.914ThrTrp: 0.914 ± 0.2
1.611ThrTyr: 1.611 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
6.14ValAla: 6.14 ± 0.734
0.697ValCys: 0.697 ± 0.229
4.093ValAsp: 4.093 ± 0.355
4.006ValGlu: 4.006 ± 0.32
2.003ValPhe: 2.003 ± 0.309
5.138ValGly: 5.138 ± 0.562
1.872ValHis: 1.872 ± 0.307
3.527ValIle: 3.527 ± 0.55
3.788ValLys: 3.788 ± 0.373
5.225ValLeu: 5.225 ± 0.507
2.221ValMet: 2.221 ± 0.307
3.396ValAsn: 3.396 ± 0.457
3.396ValPro: 3.396 ± 0.499
3.353ValGln: 3.353 ± 0.551
3.614ValArg: 3.614 ± 0.313
4.833ValSer: 4.833 ± 0.41
3.745ValThr: 3.745 ± 0.468
4.354ValVal: 4.354 ± 0.556
0.784ValTrp: 0.784 ± 0.155
2.003ValTyr: 2.003 ± 0.271
0.0ValXaa: 0.0 ± 0.0
Trp
1.263TrpAla: 1.263 ± 0.215
0.174TrpCys: 0.174 ± 0.086
1.132TrpAsp: 1.132 ± 0.246
0.61TrpGlu: 0.61 ± 0.187
0.61TrpPhe: 0.61 ± 0.195
0.914TrpGly: 0.914 ± 0.174
0.479TrpHis: 0.479 ± 0.133
0.914TrpIle: 0.914 ± 0.186
1.132TrpLys: 1.132 ± 0.205
0.958TrpLeu: 0.958 ± 0.203
0.435TrpMet: 0.435 ± 0.15
0.61TrpAsn: 0.61 ± 0.172
0.392TrpPro: 0.392 ± 0.118
0.348TrpGln: 0.348 ± 0.119
0.61TrpArg: 0.61 ± 0.146
0.827TrpSer: 0.827 ± 0.171
0.435TrpThr: 0.435 ± 0.12
0.784TrpVal: 0.784 ± 0.149
0.174TrpTrp: 0.174 ± 0.078
0.479TrpTyr: 0.479 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.959TyrAla: 1.959 ± 0.238
0.392TyrCys: 0.392 ± 0.134
2.09TyrAsp: 2.09 ± 0.242
1.872TyrGlu: 1.872 ± 0.335
0.827TyrPhe: 0.827 ± 0.213
2.438TyrGly: 2.438 ± 0.388
1.132TyrHis: 1.132 ± 0.271
1.742TyrIle: 1.742 ± 0.349
1.829TyrLys: 1.829 ± 0.321
2.351TyrLeu: 2.351 ± 0.368
0.914TyrMet: 0.914 ± 0.175
1.48TyrAsn: 1.48 ± 0.21
1.176TyrPro: 1.176 ± 0.296
2.264TyrGln: 2.264 ± 0.336
1.568TyrArg: 1.568 ± 0.24
1.611TyrSer: 1.611 ± 0.283
0.958TyrThr: 0.958 ± 0.195
2.395TyrVal: 2.395 ± 0.304
0.435TyrTrp: 0.435 ± 0.118
1.089TyrTyr: 1.089 ± 0.252
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (22967 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski