Amino acid dipepetide frequency for Pseudomonas phage DL64

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.08AlaAla: 9.08 ± 1.054
1.124AlaCys: 1.124 ± 0.29
5.08AlaAsp: 5.08 ± 0.549
6.428AlaGlu: 6.428 ± 0.653
3.012AlaPhe: 3.012 ± 0.335
6.563AlaGly: 6.563 ± 0.687
1.124AlaHis: 1.124 ± 0.182
3.866AlaIle: 3.866 ± 0.513
6.158AlaLys: 6.158 ± 0.669
8.406AlaLeu: 8.406 ± 0.826
2.652AlaMet: 2.652 ± 0.315
4.27AlaAsn: 4.27 ± 0.524
2.562AlaPro: 2.562 ± 0.438
4.36AlaGln: 4.36 ± 0.6
4.765AlaArg: 4.765 ± 0.527
4.855AlaSer: 4.855 ± 0.475
5.754AlaThr: 5.754 ± 0.681
7.462AlaVal: 7.462 ± 0.572
1.034AlaTrp: 1.034 ± 0.269
2.877AlaTyr: 2.877 ± 0.349
0.0AlaXaa: 0.0 ± 0.0
Cys
0.494CysAla: 0.494 ± 0.135
0.27CysCys: 0.27 ± 0.124
0.584CysAsp: 0.584 ± 0.175
0.584CysGlu: 0.584 ± 0.223
0.315CysPhe: 0.315 ± 0.159
0.764CysGly: 0.764 ± 0.261
0.315CysHis: 0.315 ± 0.125
0.45CysIle: 0.45 ± 0.142
0.494CysLys: 0.494 ± 0.154
0.854CysLeu: 0.854 ± 0.233
0.18CysMet: 0.18 ± 0.084
0.674CysAsn: 0.674 ± 0.259
0.27CysPro: 0.27 ± 0.121
0.405CysGln: 0.405 ± 0.141
0.45CysArg: 0.45 ± 0.15
0.584CysSer: 0.584 ± 0.183
0.36CysThr: 0.36 ± 0.137
0.674CysVal: 0.674 ± 0.194
0.18CysTrp: 0.18 ± 0.098
0.315CysTyr: 0.315 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
5.979AspAla: 5.979 ± 0.536
0.405AspCys: 0.405 ± 0.149
3.057AspAsp: 3.057 ± 0.491
4.091AspGlu: 4.091 ± 0.574
2.652AspPhe: 2.652 ± 0.468
4.181AspGly: 4.181 ± 0.383
1.214AspHis: 1.214 ± 0.277
3.461AspIle: 3.461 ± 0.353
2.967AspLys: 2.967 ± 0.352
5.619AspLeu: 5.619 ± 0.566
1.843AspMet: 1.843 ± 0.351
2.562AspAsn: 2.562 ± 0.314
3.147AspPro: 3.147 ± 0.465
2.742AspGln: 2.742 ± 0.387
2.967AspArg: 2.967 ± 0.408
3.866AspSer: 3.866 ± 0.387
3.237AspThr: 3.237 ± 0.305
3.506AspVal: 3.506 ± 0.324
0.899AspTrp: 0.899 ± 0.194
1.978AspTyr: 1.978 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
7.642GluAla: 7.642 ± 0.972
0.674GluCys: 0.674 ± 0.238
4.046GluAsp: 4.046 ± 0.395
5.844GluGlu: 5.844 ± 1.117
2.337GluPhe: 2.337 ± 0.342
4.181GluGly: 4.181 ± 0.52
1.663GluHis: 1.663 ± 0.272
3.686GluIle: 3.686 ± 0.481
3.776GluLys: 3.776 ± 0.445
6.203GluLeu: 6.203 ± 0.489
2.742GluMet: 2.742 ± 0.314
2.427GluAsn: 2.427 ± 0.288
2.293GluPro: 2.293 ± 0.385
4.001GluGln: 4.001 ± 0.711
3.281GluArg: 3.281 ± 0.464
3.821GluSer: 3.821 ± 0.488
2.742GluThr: 2.742 ± 0.306
4.945GluVal: 4.945 ± 0.438
0.989GluTrp: 0.989 ± 0.242
2.248GluTyr: 2.248 ± 0.347
0.0GluXaa: 0.0 ± 0.0
Phe
2.382PheAla: 2.382 ± 0.3
0.405PheCys: 0.405 ± 0.144
2.652PheAsp: 2.652 ± 0.368
1.663PheGlu: 1.663 ± 0.351
1.888PhePhe: 1.888 ± 0.335
2.517PheGly: 2.517 ± 0.334
0.629PheHis: 0.629 ± 0.198
2.337PheIle: 2.337 ± 0.264
2.158PheLys: 2.158 ± 0.26
2.787PheLeu: 2.787 ± 0.432
1.169PheMet: 1.169 ± 0.245
2.203PheAsn: 2.203 ± 0.246
1.214PhePro: 1.214 ± 0.311
2.158PheGln: 2.158 ± 0.328
2.472PheArg: 2.472 ± 0.376
2.652PheSer: 2.652 ± 0.35
2.382PheThr: 2.382 ± 0.373
2.607PheVal: 2.607 ± 0.334
0.36PheTrp: 0.36 ± 0.127
1.304PheTyr: 1.304 ± 0.275
0.0PheXaa: 0.0 ± 0.0
Gly
5.529GlyAla: 5.529 ± 0.414
0.539GlyCys: 0.539 ± 0.172
3.416GlyAsp: 3.416 ± 0.413
4.9GlyGlu: 4.9 ± 0.549
3.506GlyPhe: 3.506 ± 0.403
4.225GlyGly: 4.225 ± 0.51
1.079GlyHis: 1.079 ± 0.23
3.596GlyIle: 3.596 ± 0.385
4.136GlyLys: 4.136 ± 0.493
5.889GlyLeu: 5.889 ± 0.611
2.517GlyMet: 2.517 ± 0.292
3.012GlyAsn: 3.012 ± 0.395
1.798GlyPro: 1.798 ± 0.244
3.102GlyGln: 3.102 ± 0.485
3.102GlyArg: 3.102 ± 0.363
4.001GlySer: 4.001 ± 0.364
3.911GlyThr: 3.911 ± 0.502
5.574GlyVal: 5.574 ± 0.56
1.214GlyTrp: 1.214 ± 0.202
2.337GlyTyr: 2.337 ± 0.342
0.0GlyXaa: 0.0 ± 0.0
His
1.708HisAla: 1.708 ± 0.247
0.135HisCys: 0.135 ± 0.085
0.944HisAsp: 0.944 ± 0.228
1.304HisGlu: 1.304 ± 0.225
1.169HisPhe: 1.169 ± 0.277
1.304HisGly: 1.304 ± 0.271
0.539HisHis: 0.539 ± 0.151
1.214HisIle: 1.214 ± 0.272
0.944HisLys: 0.944 ± 0.204
1.798HisLeu: 1.798 ± 0.403
0.629HisMet: 0.629 ± 0.164
0.899HisAsn: 0.899 ± 0.203
0.584HisPro: 0.584 ± 0.169
0.494HisGln: 0.494 ± 0.15
1.034HisArg: 1.034 ± 0.239
1.169HisSer: 1.169 ± 0.294
0.584HisThr: 0.584 ± 0.139
1.304HisVal: 1.304 ± 0.263
0.45HisTrp: 0.45 ± 0.212
0.719HisTyr: 0.719 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
4.9IleAla: 4.9 ± 0.406
0.405IleCys: 0.405 ± 0.136
3.057IleAsp: 3.057 ± 0.512
3.192IleGlu: 3.192 ± 0.359
1.753IlePhe: 1.753 ± 0.249
3.506IleGly: 3.506 ± 0.483
1.349IleHis: 1.349 ± 0.335
1.978IleIle: 1.978 ± 0.352
3.237IleLys: 3.237 ± 0.335
3.731IleLeu: 3.731 ± 0.47
1.169IleMet: 1.169 ± 0.253
2.787IleAsn: 2.787 ± 0.347
1.978IlePro: 1.978 ± 0.375
2.607IleGln: 2.607 ± 0.373
3.596IleArg: 3.596 ± 0.352
2.293IleSer: 2.293 ± 0.298
3.237IleThr: 3.237 ± 0.364
3.326IleVal: 3.326 ± 0.352
0.27IleTrp: 0.27 ± 0.124
1.483IleTyr: 1.483 ± 0.324
0.0IleXaa: 0.0 ± 0.0
Lys
6.293LysAla: 6.293 ± 0.552
0.494LysCys: 0.494 ± 0.158
4.091LysAsp: 4.091 ± 0.49
4.225LysGlu: 4.225 ± 0.571
1.618LysPhe: 1.618 ± 0.251
3.551LysGly: 3.551 ± 0.488
1.079LysHis: 1.079 ± 0.243
2.652LysIle: 2.652 ± 0.359
3.686LysLys: 3.686 ± 0.619
5.439LysLeu: 5.439 ± 0.522
1.708LysMet: 1.708 ± 0.268
1.933LysAsn: 1.933 ± 0.297
2.742LysPro: 2.742 ± 0.383
2.293LysGln: 2.293 ± 0.305
3.192LysArg: 3.192 ± 0.363
3.281LysSer: 3.281 ± 0.347
3.371LysThr: 3.371 ± 0.324
4.54LysVal: 4.54 ± 0.429
0.584LysTrp: 0.584 ± 0.136
2.382LysTyr: 2.382 ± 0.375
0.0LysXaa: 0.0 ± 0.0
Leu
7.372LeuAla: 7.372 ± 0.681
1.214LeuCys: 1.214 ± 0.336
6.338LeuAsp: 6.338 ± 0.635
5.754LeuGlu: 5.754 ± 0.559
3.057LeuPhe: 3.057 ± 0.392
5.169LeuGly: 5.169 ± 0.557
1.798LeuHis: 1.798 ± 0.285
4.36LeuIle: 4.36 ± 0.59
5.574LeuLys: 5.574 ± 0.521
8.316LeuLeu: 8.316 ± 0.848
2.922LeuMet: 2.922 ± 0.332
4.181LeuAsn: 4.181 ± 0.433
4.315LeuPro: 4.315 ± 0.449
3.057LeuGln: 3.057 ± 0.325
4.855LeuArg: 4.855 ± 0.527
5.529LeuSer: 5.529 ± 0.468
5.259LeuThr: 5.259 ± 0.482
6.428LeuVal: 6.428 ± 0.652
0.944LeuTrp: 0.944 ± 0.225
2.517LeuTyr: 2.517 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
3.057MetAla: 3.057 ± 0.371
0.225MetCys: 0.225 ± 0.108
2.293MetAsp: 2.293 ± 0.336
1.753MetGlu: 1.753 ± 0.221
1.259MetPhe: 1.259 ± 0.218
1.888MetGly: 1.888 ± 0.298
0.719MetHis: 0.719 ± 0.2
1.528MetIle: 1.528 ± 0.259
1.214MetLys: 1.214 ± 0.263
3.012MetLeu: 3.012 ± 0.341
0.854MetMet: 0.854 ± 0.213
1.349MetAsn: 1.349 ± 0.182
1.753MetPro: 1.753 ± 0.309
1.528MetGln: 1.528 ± 0.246
1.663MetArg: 1.663 ± 0.266
2.248MetSer: 2.248 ± 0.375
2.427MetThr: 2.427 ± 0.316
1.438MetVal: 1.438 ± 0.328
0.27MetTrp: 0.27 ± 0.14
0.719MetTyr: 0.719 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
4.136AsnAla: 4.136 ± 0.518
0.405AsnCys: 0.405 ± 0.147
2.113AsnAsp: 2.113 ± 0.379
3.147AsnGlu: 3.147 ± 0.414
1.573AsnPhe: 1.573 ± 0.338
3.776AsnGly: 3.776 ± 0.558
0.899AsnHis: 0.899 ± 0.259
2.337AsnIle: 2.337 ± 0.284
3.237AsnLys: 3.237 ± 0.363
4.585AsnLeu: 4.585 ± 0.49
1.798AsnMet: 1.798 ± 0.306
2.113AsnAsn: 2.113 ± 0.457
2.562AsnPro: 2.562 ± 0.318
1.349AsnGln: 1.349 ± 0.22
2.382AsnArg: 2.382 ± 0.34
2.472AsnSer: 2.472 ± 0.327
3.057AsnThr: 3.057 ± 0.381
2.337AsnVal: 2.337 ± 0.265
0.539AsnTrp: 0.539 ± 0.163
1.214AsnTyr: 1.214 ± 0.182
0.0AsnXaa: 0.0 ± 0.0
Pro
3.596ProAla: 3.596 ± 0.435
0.225ProCys: 0.225 ± 0.103
2.293ProAsp: 2.293 ± 0.384
3.371ProGlu: 3.371 ± 0.458
1.753ProPhe: 1.753 ± 0.247
3.057ProGly: 3.057 ± 0.392
0.539ProHis: 0.539 ± 0.185
1.843ProIle: 1.843 ± 0.239
1.933ProLys: 1.933 ± 0.33
3.281ProLeu: 3.281 ± 0.351
1.259ProMet: 1.259 ± 0.255
1.708ProAsn: 1.708 ± 0.259
0.899ProPro: 0.899 ± 0.295
1.978ProGln: 1.978 ± 0.301
1.438ProArg: 1.438 ± 0.292
2.607ProSer: 2.607 ± 0.327
2.742ProThr: 2.742 ± 0.486
3.506ProVal: 3.506 ± 0.372
0.315ProTrp: 0.315 ± 0.128
1.394ProTyr: 1.394 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
4.81GlnAla: 4.81 ± 0.662
0.18GlnCys: 0.18 ± 0.089
2.742GlnAsp: 2.742 ± 0.414
4.091GlnGlu: 4.091 ± 0.514
1.663GlnPhe: 1.663 ± 0.216
2.248GlnGly: 2.248 ± 0.371
0.584GlnHis: 0.584 ± 0.135
2.562GlnIle: 2.562 ± 0.313
2.472GlnLys: 2.472 ± 0.357
4.315GlnLeu: 4.315 ± 0.474
1.394GlnMet: 1.394 ± 0.26
1.573GlnAsn: 1.573 ± 0.303
1.349GlnPro: 1.349 ± 0.217
1.978GlnGln: 1.978 ± 0.383
2.472GlnArg: 2.472 ± 0.392
2.023GlnSer: 2.023 ± 0.299
2.607GlnThr: 2.607 ± 0.478
4.046GlnVal: 4.046 ± 0.581
0.405GlnTrp: 0.405 ± 0.143
1.304GlnTyr: 1.304 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
5.259ArgAla: 5.259 ± 0.621
0.539ArgCys: 0.539 ± 0.206
3.326ArgAsp: 3.326 ± 0.392
3.686ArgGlu: 3.686 ± 0.541
2.203ArgPhe: 2.203 ± 0.345
3.506ArgGly: 3.506 ± 0.427
1.169ArgHis: 1.169 ± 0.291
2.562ArgIle: 2.562 ± 0.354
3.057ArgLys: 3.057 ± 0.412
4.99ArgLeu: 4.99 ± 0.474
1.438ArgMet: 1.438 ± 0.184
2.427ArgAsn: 2.427 ± 0.336
1.843ArgPro: 1.843 ± 0.305
2.787ArgGln: 2.787 ± 0.421
3.371ArgArg: 3.371 ± 0.504
3.461ArgSer: 3.461 ± 0.383
2.517ArgThr: 2.517 ± 0.311
3.641ArgVal: 3.641 ± 0.325
0.719ArgTrp: 0.719 ± 0.188
1.349ArgTyr: 1.349 ± 0.245
0.0ArgXaa: 0.0 ± 0.0
Ser
4.495SerAla: 4.495 ± 0.483
0.36SerCys: 0.36 ± 0.128
3.776SerAsp: 3.776 ± 0.422
4.136SerGlu: 4.136 ± 0.424
1.933SerPhe: 1.933 ± 0.359
4.72SerGly: 4.72 ± 0.425
1.034SerHis: 1.034 ± 0.208
2.562SerIle: 2.562 ± 0.276
3.821SerLys: 3.821 ± 0.45
4.765SerLeu: 4.765 ± 0.482
1.933SerMet: 1.933 ± 0.31
2.922SerAsn: 2.922 ± 0.401
2.652SerPro: 2.652 ± 0.325
2.203SerGln: 2.203 ± 0.298
3.012SerArg: 3.012 ± 0.345
2.967SerSer: 2.967 ± 0.42
3.641SerThr: 3.641 ± 0.381
2.967SerVal: 2.967 ± 0.299
0.809SerTrp: 0.809 ± 0.18
2.068SerTyr: 2.068 ± 0.224
0.0SerXaa: 0.0 ± 0.0
Thr
4.99ThrAla: 4.99 ± 0.51
0.315ThrCys: 0.315 ± 0.125
3.281ThrAsp: 3.281 ± 0.307
4.315ThrGlu: 4.315 ± 0.447
1.933ThrPhe: 1.933 ± 0.381
4.091ThrGly: 4.091 ± 0.417
1.169ThrHis: 1.169 ± 0.228
3.326ThrIle: 3.326 ± 0.405
3.686ThrLys: 3.686 ± 0.465
4.181ThrLeu: 4.181 ± 0.438
1.888ThrMet: 1.888 ± 0.274
3.326ThrAsn: 3.326 ± 0.423
2.922ThrPro: 2.922 ± 0.367
2.607ThrGln: 2.607 ± 0.379
3.102ThrArg: 3.102 ± 0.339
2.562ThrSer: 2.562 ± 0.388
4.136ThrThr: 4.136 ± 0.484
4.45ThrVal: 4.45 ± 0.452
1.304ThrTrp: 1.304 ± 0.233
1.708ThrTyr: 1.708 ± 0.452
0.0ThrXaa: 0.0 ± 0.0
Val
6.383ValAla: 6.383 ± 0.755
0.809ValCys: 0.809 ± 0.194
4.405ValAsp: 4.405 ± 0.527
4.675ValGlu: 4.675 ± 0.438
2.113ValPhe: 2.113 ± 0.364
4.945ValGly: 4.945 ± 0.411
1.259ValHis: 1.259 ± 0.223
3.371ValIle: 3.371 ± 0.473
4.091ValLys: 4.091 ± 0.301
6.428ValLeu: 6.428 ± 0.604
2.023ValMet: 2.023 ± 0.307
3.686ValAsn: 3.686 ± 0.326
3.192ValPro: 3.192 ± 0.419
3.326ValGln: 3.326 ± 0.423
4.091ValArg: 4.091 ± 0.484
3.911ValSer: 3.911 ± 0.609
4.27ValThr: 4.27 ± 0.417
4.315ValVal: 4.315 ± 0.548
0.854ValTrp: 0.854 ± 0.176
2.337ValTyr: 2.337 ± 0.361
0.0ValXaa: 0.0 ± 0.0
Trp
1.034TrpAla: 1.034 ± 0.254
0.225TrpCys: 0.225 ± 0.134
0.539TrpAsp: 0.539 ± 0.183
0.719TrpGlu: 0.719 ± 0.149
0.764TrpPhe: 0.764 ± 0.228
0.764TrpGly: 0.764 ± 0.181
0.18TrpHis: 0.18 ± 0.097
0.539TrpIle: 0.539 ± 0.152
0.674TrpLys: 0.674 ± 0.179
1.259TrpLeu: 1.259 ± 0.307
0.315TrpMet: 0.315 ± 0.119
0.45TrpAsn: 0.45 ± 0.15
0.494TrpPro: 0.494 ± 0.144
0.629TrpGln: 0.629 ± 0.208
0.584TrpArg: 0.584 ± 0.116
0.854TrpSer: 0.854 ± 0.213
0.764TrpThr: 0.764 ± 0.183
1.349TrpVal: 1.349 ± 0.273
0.315TrpTrp: 0.315 ± 0.151
0.494TrpTyr: 0.494 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.293TyrAla: 2.293 ± 0.317
0.27TyrCys: 0.27 ± 0.108
2.337TyrAsp: 2.337 ± 0.285
1.663TyrGlu: 1.663 ± 0.212
1.438TyrPhe: 1.438 ± 0.22
2.427TyrGly: 2.427 ± 0.43
0.584TyrHis: 0.584 ± 0.168
1.618TyrIle: 1.618 ± 0.388
1.843TyrLys: 1.843 ± 0.362
3.147TyrLeu: 3.147 ± 0.39
0.719TyrMet: 0.719 ± 0.194
1.528TyrAsn: 1.528 ± 0.244
1.169TyrPro: 1.169 ± 0.304
1.214TyrGln: 1.214 ± 0.229
2.023TyrArg: 2.023 ± 0.27
1.618TyrSer: 1.618 ± 0.195
2.293TyrThr: 2.293 ± 0.369
2.068TyrVal: 2.068 ± 0.325
0.494TyrTrp: 0.494 ± 0.147
1.349TyrTyr: 1.349 ± 0.25
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (22247 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski