Nocardioides sp. Root190

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8Q6X4|A0A0Q8Q6X4_9ACTN Ribose import ATP-binding protein RbsA OS=Nocardioides sp. Root190 OX=1736488 GN=rbsA PE=3 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84LSSRR6 pKa = 11.84FVSLSALALLAPAFAACSAFSEE28 pKa = 4.81GGGDD32 pKa = 3.42TTSGNGVSVAAAFYY46 pKa = 8.68PLAWVVEE53 pKa = 4.78EE54 pKa = 4.18VAEE57 pKa = 4.46GTGTEE62 pKa = 4.24VEE64 pKa = 4.66LLTSPGAEE72 pKa = 3.82PHH74 pKa = 6.86DD75 pKa = 4.54LEE77 pKa = 4.64LTVKK81 pKa = 8.66QTAVVVDD88 pKa = 4.28ADD90 pKa = 3.96LVLYY94 pKa = 10.35EE95 pKa = 5.33SGFQPAVDD103 pKa = 3.51DD104 pKa = 4.18TVAQNAEE111 pKa = 4.21GATLDD116 pKa = 3.39AAGIVDD122 pKa = 6.15LIPVEE127 pKa = 4.68EE128 pKa = 4.3SAEE131 pKa = 4.03EE132 pKa = 4.24HH133 pKa = 6.65EE134 pKa = 4.36EE135 pKa = 4.0HH136 pKa = 7.17ADD138 pKa = 4.28EE139 pKa = 5.02EE140 pKa = 4.94GHH142 pKa = 7.26DD143 pKa = 4.16HH144 pKa = 7.37DD145 pKa = 5.6HH146 pKa = 7.12DD147 pKa = 4.66HH148 pKa = 7.2GGLDD152 pKa = 3.26PHH154 pKa = 6.97FWQDD158 pKa = 3.76PLRR161 pKa = 11.84MAALADD167 pKa = 3.74AVAGEE172 pKa = 4.67LGALDD177 pKa = 4.52EE178 pKa = 5.1EE179 pKa = 4.69NADD182 pKa = 3.93TYY184 pKa = 11.74DD185 pKa = 3.54EE186 pKa = 4.15NAAALRR192 pKa = 11.84TDD194 pKa = 4.9LEE196 pKa = 4.37ALDD199 pKa = 4.45AEE201 pKa = 4.84YY202 pKa = 10.76AAGLTDD208 pKa = 4.14CEE210 pKa = 4.26RR211 pKa = 11.84DD212 pKa = 3.91TIVVSHH218 pKa = 7.24DD219 pKa = 2.92AFGYY223 pKa = 8.28LEE225 pKa = 4.24KK226 pKa = 11.15YY227 pKa = 9.65GVHH230 pKa = 6.55LASIVGLSPDD240 pKa = 4.15AEE242 pKa = 4.21PTGAVLGEE250 pKa = 4.01LQEE253 pKa = 4.85LIGEE257 pKa = 4.25EE258 pKa = 4.78GITTVFSEE266 pKa = 4.46PLEE269 pKa = 4.32PALGEE274 pKa = 4.24GLATDD279 pKa = 4.72LGLTNGTLDD288 pKa = 4.75PIEE291 pKa = 4.66GLSDD295 pKa = 3.24ATSGEE300 pKa = 4.69DD301 pKa = 3.65YY302 pKa = 11.03LSLMTSNLAAIQTANGCRR320 pKa = 3.41

Molecular weight: 33.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8Q533|A0A0Q8Q533_9ACTN Dehydrogenase OS=Nocardioides sp. Root190 OX=1736488 GN=ASE01_09150 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 9.98VRR4 pKa = 11.84NSIRR8 pKa = 11.84SLKK11 pKa = 8.78NQPGAQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VFVINKK30 pKa = 7.77QNPRR34 pKa = 11.84MKK36 pKa = 10.39GRR38 pKa = 11.84QGG40 pKa = 3.03

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4603

0

4603

1497011

37

2683

325.2

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.994 ± 0.051

0.723 ± 0.009

6.542 ± 0.029

5.853 ± 0.037

2.843 ± 0.021

9.212 ± 0.038

2.133 ± 0.02

3.845 ± 0.025

2.074 ± 0.023

10.217 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.014

1.879 ± 0.02

5.465 ± 0.026

2.76 ± 0.017

7.317 ± 0.04

5.416 ± 0.027

6.187 ± 0.035

9.318 ± 0.036

1.512 ± 0.014

1.912 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski