Salisaeta icosahedral phage 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA phages

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1ZBF2|I1ZBF2_9VIRU Uncharacterized protein OS=Salisaeta icosahedral phage 1 OX=1183239 PE=4 SV=1
MM1 pKa = 7.65TDD3 pKa = 3.08AKK5 pKa = 10.0EE6 pKa = 4.01ARR8 pKa = 11.84YY9 pKa = 7.76TLAGRR14 pKa = 11.84HH15 pKa = 5.51ANRR18 pKa = 11.84SSADD22 pKa = 3.36SFFEE26 pKa = 4.0QVLSEE31 pKa = 5.48SYY33 pKa = 9.15STWDD37 pKa = 3.36DD38 pKa = 3.87AEE40 pKa = 4.39TLSEE44 pKa = 4.36LSYY47 pKa = 11.32EE48 pKa = 4.49EE49 pKa = 4.51YY50 pKa = 10.99FEE52 pKa = 4.72ATMADD57 pKa = 3.23GALYY61 pKa = 10.01NSPGFEE67 pKa = 4.56DD68 pKa = 5.16LLIASDD74 pKa = 3.71EE75 pKa = 4.26KK76 pKa = 11.44EE77 pKa = 3.7EE78 pKa = 5.24AEE80 pKa = 5.09ALFQRR85 pKa = 11.84VNARR89 pKa = 11.84CYY91 pKa = 9.33IAHH94 pKa = 6.87LEE96 pKa = 3.98EE97 pKa = 4.53SAAEE101 pKa = 4.06EE102 pKa = 4.45GDD104 pKa = 3.66LHH106 pKa = 7.15VVQTDD111 pKa = 3.9GLGHH115 pKa = 6.15SSGYY119 pKa = 9.58PYY121 pKa = 10.79LIASFDD127 pKa = 3.83DD128 pKa = 3.61EE129 pKa = 4.14EE130 pKa = 4.83AAEE133 pKa = 4.31RR134 pKa = 11.84FCGALEE140 pKa = 3.97MEE142 pKa = 4.81YY143 pKa = 11.06DD144 pKa = 3.87DD145 pKa = 5.24NSYY148 pKa = 11.01SIYY151 pKa = 10.38KK152 pKa = 10.01IEE154 pKa = 4.43GDD156 pKa = 4.24SVFDD160 pKa = 4.13YY161 pKa = 10.45YY162 pKa = 10.82TGQPVDD168 pKa = 3.56YY169 pKa = 10.54DD170 pKa = 3.51AA171 pKa = 6.86

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1ZBE9|I1ZBE9_9VIRU Uncharacterized protein OS=Salisaeta icosahedral phage 1 OX=1183239 PE=4 SV=1
MM1 pKa = 6.72THH3 pKa = 5.11HH4 pKa = 6.68TVRR7 pKa = 11.84KK8 pKa = 9.36KK9 pKa = 10.52RR10 pKa = 11.84SEE12 pKa = 4.19HH13 pKa = 6.27KK14 pKa = 10.03GGAMTTPAEE23 pKa = 3.86IRR25 pKa = 11.84ALRR28 pKa = 11.84RR29 pKa = 11.84DD30 pKa = 3.24LGLRR34 pKa = 11.84QVDD37 pKa = 4.01FCEE40 pKa = 4.02VLGVHH45 pKa = 5.34QTTLSDD51 pKa = 3.59WEE53 pKa = 4.51SGRR56 pKa = 11.84SSPDD60 pKa = 2.83LYY62 pKa = 11.1KK63 pKa = 10.21RR64 pKa = 11.84TVLEE68 pKa = 3.56QLRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84LRR75 pKa = 11.84EE76 pKa = 3.71AEE78 pKa = 3.81RR79 pKa = 11.84EE80 pKa = 3.85QRR82 pKa = 11.84TEE84 pKa = 3.68QFRR87 pKa = 11.84SKK89 pKa = 10.78LLRR92 pKa = 11.84ASLSTGLHH100 pKa = 5.34ALFRR104 pKa = 11.84TLFSGEE110 pKa = 3.94VKK112 pKa = 10.68

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13103

47

2319

229.9

25.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.715 ± 0.304

0.672 ± 0.124

6.922 ± 0.385

7.494 ± 0.588

3.121 ± 0.232

7.021 ± 0.416

2.152 ± 0.158

4.274 ± 0.189

4.373 ± 0.354

8.357 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.13

2.969 ± 0.178

4.793 ± 0.216

4.472 ± 0.397

7.762 ± 0.397

6.014 ± 0.24

6.022 ± 0.372

6.739 ± 0.303

1.549 ± 0.135

3.244 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski