Harryflintia acetispora

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Harryflintia

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2808 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E2STY8|A0A3E2STY8_9FIRM Uncharacterized protein OS=Harryflintia acetispora OX=1849041 GN=DW086_10200 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.43KK3 pKa = 10.6VMATLLAGCLLLGSVLSASAASISKK28 pKa = 10.26SSSSVSGYY36 pKa = 10.74SLTVGPGDD44 pKa = 4.77DD45 pKa = 3.48NAEE48 pKa = 4.19YY49 pKa = 10.92SDD51 pKa = 4.34GAPEE55 pKa = 4.32IYY57 pKa = 9.86TYY59 pKa = 10.11GTDD62 pKa = 3.21SMKK65 pKa = 10.41EE66 pKa = 3.79AEE68 pKa = 4.14IAIIPEE74 pKa = 3.96SGYY77 pKa = 10.8EE78 pKa = 3.8ITDD81 pKa = 2.92VDD83 pKa = 4.17VSVNTSNISASYY95 pKa = 9.64KK96 pKa = 10.75KK97 pKa = 10.37NDD99 pKa = 3.59GTVSDD104 pKa = 4.88PGVLTIKK111 pKa = 10.46GKK113 pKa = 10.07AGVRR117 pKa = 11.84VFEE120 pKa = 4.63LEE122 pKa = 4.83DD123 pKa = 3.61YY124 pKa = 11.13EE125 pKa = 5.91LDD127 pKa = 4.44LDD129 pKa = 4.05ITLEE133 pKa = 4.35DD134 pKa = 4.53DD135 pKa = 3.66ATGDD139 pKa = 3.81EE140 pKa = 4.51YY141 pKa = 11.55DD142 pKa = 3.66VTITIYY148 pKa = 11.03GEE150 pKa = 3.83EE151 pKa = 4.05LTAYY155 pKa = 9.74SPSRR159 pKa = 11.84EE160 pKa = 4.16VEE162 pKa = 3.67SDD164 pKa = 2.94EE165 pKa = 4.72TYY167 pKa = 10.59TITKK171 pKa = 10.05SLGCIFEE178 pKa = 3.99FDD180 pKa = 3.69EE181 pKa = 5.35YY182 pKa = 10.54IDD184 pKa = 3.59EE185 pKa = 4.25EE186 pKa = 4.3TRR188 pKa = 11.84IRR190 pKa = 11.84VDD192 pKa = 4.67DD193 pKa = 6.03DD194 pKa = 3.37IDD196 pKa = 4.57LYY198 pKa = 11.74FNGNYY203 pKa = 7.95GTDD206 pKa = 3.65YY207 pKa = 11.22EE208 pKa = 4.29NLRR211 pKa = 11.84VVTDD215 pKa = 4.54SISEE219 pKa = 3.88IDD221 pKa = 3.56KK222 pKa = 11.02ALSDD226 pKa = 4.63LYY228 pKa = 11.28VDD230 pKa = 4.23YY231 pKa = 11.3YY232 pKa = 11.7DD233 pKa = 6.35FIASPKK239 pKa = 8.73FASAVEE245 pKa = 4.04VEE247 pKa = 4.55IKK249 pKa = 10.65AGTEE253 pKa = 3.86AYY255 pKa = 9.51IYY257 pKa = 9.87EE258 pKa = 4.35YY259 pKa = 10.98NKK261 pKa = 9.31KK262 pKa = 8.67TGDD265 pKa = 3.48LTLVDD270 pKa = 5.08AEE272 pKa = 4.7YY273 pKa = 10.94DD274 pKa = 3.88GNWIFEE280 pKa = 4.33TKK282 pKa = 9.82TLGTYY287 pKa = 9.92IVTDD291 pKa = 3.66EE292 pKa = 5.16EE293 pKa = 4.43IDD295 pKa = 3.57EE296 pKa = 4.71SMLSTSTADD305 pKa = 3.68EE306 pKa = 4.99DD307 pKa = 4.1STDD310 pKa = 3.37DD311 pKa = 3.87TEE313 pKa = 5.34YY314 pKa = 11.61DD315 pKa = 3.88DD316 pKa = 4.55VDD318 pKa = 4.04SVSDD322 pKa = 3.97SEE324 pKa = 4.41KK325 pKa = 10.17TNPGTGAAGSPALAVLLGIGSLCAAGLAAYY355 pKa = 9.49RR356 pKa = 11.84KK357 pKa = 9.36AARR360 pKa = 3.98

Molecular weight: 38.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E2SYB4|A0A3E2SYB4_9FIRM Type IV secretory system conjugative DNA transfer family protein OS=Harryflintia acetispora OX=1849041 GN=DW086_07850 PE=3 SV=1
MM1 pKa = 7.62GGTLRR6 pKa = 11.84RR7 pKa = 11.84TSKK10 pKa = 7.46TTLWSYY16 pKa = 11.37DD17 pKa = 2.92SRR19 pKa = 11.84ASGDD23 pKa = 3.43RR24 pKa = 11.84NVPIARR30 pKa = 11.84GAWQMWRR37 pKa = 11.84VNFSKK42 pKa = 10.6SDD44 pKa = 3.31TMRR47 pKa = 11.84WQLRR51 pKa = 11.84LPAHH55 pKa = 5.91SCDD58 pKa = 3.76FEE60 pKa = 5.4SNNHH64 pKa = 5.17IFDD67 pKa = 4.04RR68 pKa = 11.84KK69 pKa = 10.34GDD71 pKa = 4.11HH72 pKa = 6.04LWKK75 pKa = 10.82NN76 pKa = 3.6

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2808

0

2808

875103

27

10091

311.6

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.157 ± 0.043

1.736 ± 0.026

5.23 ± 0.038

6.975 ± 0.057

3.945 ± 0.038

8.336 ± 0.046

1.727 ± 0.02

5.85 ± 0.046

4.763 ± 0.046

10.23 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.03

3.171 ± 0.033

4.241 ± 0.03

3.481 ± 0.03

5.852 ± 0.052

5.997 ± 0.049

5.055 ± 0.056

7.052 ± 0.041

0.921 ± 0.015

3.609 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski