Cocleimonas flava

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Cocleimonas

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2P7P3|A0A4V2P7P3_9GAMM 50S ribosomal protein L16 3-hydroxylase OS=Cocleimonas flava OX=634765 GN=EV695_3346 PE=4 SV=1
MM1 pKa = 7.49SKK3 pKa = 10.18QVFKK7 pKa = 10.56TVFIFFILQLLAVTSISAAVPVNDD31 pKa = 3.93EE32 pKa = 4.88LGNATIIGAFPFTDD46 pKa = 3.32TVSAADD52 pKa = 3.75SDD54 pKa = 4.54DD55 pKa = 3.68ATSEE59 pKa = 4.03ADD61 pKa = 3.54EE62 pKa = 5.11LEE64 pKa = 4.61CSSGFYY70 pKa = 10.61NGTWWYY76 pKa = 10.98SVTLSEE82 pKa = 5.02SGEE85 pKa = 4.1LTADD89 pKa = 3.31AMSLNPALILPTDD102 pKa = 3.95MSNQTAVNVAIYY114 pKa = 10.53SGTTHH119 pKa = 7.84PLTEE123 pKa = 4.44LACEE127 pKa = 4.44GSFVRR132 pKa = 11.84VPDD135 pKa = 4.46PSDD138 pKa = 3.37PGYY141 pKa = 9.82TMVDD145 pKa = 2.87ITQSIINPTVTALVPAGTYY164 pKa = 10.16YY165 pKa = 10.38ILVGNSTNTPTVGDD179 pKa = 3.58IQVNVAFTPAALPAPVAEE197 pKa = 4.96NIPVNNPLALLLLILGLFSITLMKK221 pKa = 10.35KK222 pKa = 10.13RR223 pKa = 11.84NQQ225 pKa = 3.22

Molecular weight: 23.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1ESE4|A0A4R1ESE4_9GAMM Uncharacterized protein OS=Cocleimonas flava OX=634765 GN=EV695_3330 PE=4 SV=1
MM1 pKa = 7.53NLPLLFSFSPSWLTQGLQHH20 pKa = 6.88PVLTPSHH27 pKa = 7.02AILICSLAFLIGQQSKK43 pKa = 10.88LPIHH47 pKa = 6.71IFLLFISSIAGMLLNYY63 pKa = 9.72FVFPGTRR70 pKa = 11.84LEE72 pKa = 4.11LLLLGMALIIGLLVVIRR89 pKa = 11.84IKK91 pKa = 10.46QLPPLILIISLLLATLTGLFIGIDD115 pKa = 3.72SQPISIPGLRR125 pKa = 11.84TNSVINWHH133 pKa = 6.86LGAVITITLSTLILSLIAQLLRR155 pKa = 11.84RR156 pKa = 11.84FGDD159 pKa = 4.27GIVLRR164 pKa = 11.84VLGSWVATSALITLTLQYY182 pKa = 11.56AKK184 pKa = 10.29II185 pKa = 3.93

Molecular weight: 20.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3987

0

3987

1293055

29

3686

324.3

36.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.091 ± 0.041

0.894 ± 0.012

5.859 ± 0.037

6.385 ± 0.042

4.131 ± 0.029

6.824 ± 0.04

2.075 ± 0.018

7.041 ± 0.037

6.293 ± 0.05

9.889 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.598 ± 0.021

4.728 ± 0.035

3.978 ± 0.026

3.719 ± 0.028

4.128 ± 0.03

6.923 ± 0.034

5.67 ± 0.047

6.586 ± 0.036

1.181 ± 0.014

3.007 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski