Tortoise microvirus 75

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WA06|A0A4P8WA06_9VIRU Peptidase_M15_4 domain-containing protein OS=Tortoise microvirus 75 OX=2583182 PE=4 SV=1
MM1 pKa = 7.61GEE3 pKa = 3.9GSAALAEE10 pKa = 4.26MASTGIGTRR19 pKa = 11.84VIHH22 pKa = 6.88DD23 pKa = 3.77ANGVVGLQFTSPVQEE38 pKa = 4.59ASSQGSPSRR47 pKa = 11.84ATEE50 pKa = 4.04PGNVGAVEE58 pKa = 4.07RR59 pKa = 11.84RR60 pKa = 11.84QEE62 pKa = 3.8AGVIRR67 pKa = 11.84EE68 pKa = 3.93TAYY71 pKa = 10.75GSVAGDD77 pKa = 3.61ALAGARR83 pKa = 11.84QEE85 pKa = 3.96ATLEE89 pKa = 3.95NRR91 pKa = 11.84GAKK94 pKa = 10.05APALKK99 pKa = 9.3STASSRR105 pKa = 11.84GAFVASFVRR114 pKa = 11.84DD115 pKa = 3.36WNFSGKK121 pKa = 9.82DD122 pKa = 3.46DD123 pKa = 4.34PVQDD127 pKa = 3.43MVFAGTHH134 pKa = 4.45WAANPKK140 pKa = 8.2FTNGDD145 pKa = 3.77LVQTRR150 pKa = 11.84YY151 pKa = 10.44GDD153 pKa = 3.68SEE155 pKa = 4.14IVQEE159 pKa = 4.38VVGLSILGADD169 pKa = 3.28IGFNAARR176 pKa = 11.84AVFGPNVQNMGPQARR191 pKa = 11.84LDD193 pKa = 3.71ALGAMAGKK201 pKa = 9.6GAHH204 pKa = 6.08SAIQGAIDD212 pKa = 3.12TGFNLGDD219 pKa = 3.74AVRR222 pKa = 11.84EE223 pKa = 3.75AAAQNKK229 pKa = 8.02LHH231 pKa = 6.14EE232 pKa = 4.5EE233 pKa = 4.33RR234 pKa = 11.84YY235 pKa = 9.81NAAKK239 pKa = 10.55DD240 pKa = 3.73AFNEE244 pKa = 3.91AWDD247 pKa = 3.9YY248 pKa = 11.38RR249 pKa = 11.84EE250 pKa = 4.18KK251 pKa = 10.91LQEE254 pKa = 3.92QEE256 pKa = 4.38AVRR259 pKa = 11.84QQGGNIGGFFF269 pKa = 3.59

Molecular weight: 28.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8WA59|A0A4P8WA59_9VIRU Replication initiation protein OS=Tortoise microvirus 75 OX=2583182 PE=4 SV=1
MM1 pKa = 7.65AKK3 pKa = 10.14HH4 pKa = 5.79SPKK7 pKa = 10.05NQVYY11 pKa = 10.26SKK13 pKa = 9.28PPKK16 pKa = 10.1GGFVYY21 pKa = 10.39EE22 pKa = 4.23PEE24 pKa = 4.1EE25 pKa = 4.33VFPGDD30 pKa = 3.71YY31 pKa = 10.1QEE33 pKa = 4.67HH34 pKa = 5.83EE35 pKa = 4.53NYY37 pKa = 10.11RR38 pKa = 11.84EE39 pKa = 3.99ALRR42 pKa = 11.84EE43 pKa = 4.03LKK45 pKa = 10.59DD46 pKa = 3.36PAMLRR51 pKa = 11.84SKK53 pKa = 10.43KK54 pKa = 9.59YY55 pKa = 10.21QEE57 pKa = 4.16QQWRR61 pKa = 11.84AIRR64 pKa = 11.84IGANPQLLLHH74 pKa = 6.1MRR76 pKa = 11.84LMVARR81 pKa = 11.84FKK83 pKa = 11.07QVGIPVFPSEE93 pKa = 3.87IVRR96 pKa = 11.84SHH98 pKa = 5.8EE99 pKa = 3.99RR100 pKa = 11.84QNQLKK105 pKa = 10.58ADD107 pKa = 4.13GFSKK111 pKa = 10.9ASGAKK116 pKa = 9.5APHH119 pKa = 7.26PYY121 pKa = 9.77GCAYY125 pKa = 10.66DD126 pKa = 4.61LVHH129 pKa = 7.21SVRR132 pKa = 11.84GWNLSKK138 pKa = 10.77KK139 pKa = 6.86QWEE142 pKa = 4.6VFGHH146 pKa = 5.28VGKK149 pKa = 10.45EE150 pKa = 3.49LAIQRR155 pKa = 11.84NIPMIWGGDD164 pKa = 3.09WKK166 pKa = 10.83FYY168 pKa = 11.39DD169 pKa = 3.92PAHH172 pKa = 6.02WQLKK176 pKa = 6.16EE177 pKa = 3.54WRR179 pKa = 11.84QIMTDD184 pKa = 4.06YY185 pKa = 10.42PWPPANKK192 pKa = 10.17GIAHH196 pKa = 7.3RR197 pKa = 4.51

Molecular weight: 23.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1817

68

545

259.6

28.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.062 ± 0.981

0.495 ± 0.183

4.953 ± 0.468

6.824 ± 0.992

3.467 ± 0.243

8.586 ± 1.221

2.422 ± 0.448

3.357 ± 0.348

4.898 ± 0.876

6.604 ± 0.898

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.372

3.797 ± 0.565

5.779 ± 0.93

4.678 ± 0.535

7.1 ± 0.717

5.944 ± 0.581

4.348 ± 0.901

7.76 ± 0.838

2.146 ± 0.409

2.972 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski