Amino acid dipepetide frequency for Rhodobacter phage RcapMu

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.362AlaAla: 23.362 ± 1.806
0.638AlaCys: 0.638 ± 0.267
8.133AlaAsp: 8.133 ± 0.743
9.089AlaGlu: 9.089 ± 0.874
3.428AlaPhe: 3.428 ± 0.584
10.285AlaGly: 10.285 ± 1.035
2.073AlaHis: 2.073 ± 0.392
5.98AlaIle: 5.98 ± 0.558
5.342AlaLys: 5.342 ± 0.736
11.96AlaLeu: 11.96 ± 0.762
3.508AlaMet: 3.508 ± 0.643
2.551AlaAsn: 2.551 ± 0.495
5.98AlaPro: 5.98 ± 0.628
5.103AlaGln: 5.103 ± 0.75
9.568AlaArg: 9.568 ± 1.139
7.256AlaSer: 7.256 ± 0.78
7.176AlaThr: 7.176 ± 1.052
10.046AlaVal: 10.046 ± 1.161
1.515AlaTrp: 1.515 ± 0.315
1.674AlaTyr: 1.674 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.558CysAla: 0.558 ± 0.214
0.159CysCys: 0.159 ± 0.112
0.558CysAsp: 0.558 ± 0.241
0.319CysGlu: 0.319 ± 0.142
0.159CysPhe: 0.159 ± 0.112
0.718CysGly: 0.718 ± 0.247
0.319CysHis: 0.319 ± 0.175
0.319CysIle: 0.319 ± 0.138
0.159CysLys: 0.159 ± 0.113
0.478CysLeu: 0.478 ± 0.196
0.239CysMet: 0.239 ± 0.121
0.0CysAsn: 0.0 ± 0.0
0.478CysPro: 0.478 ± 0.233
0.08CysGln: 0.08 ± 0.076
0.797CysArg: 0.797 ± 0.278
0.638CysSer: 0.638 ± 0.249
0.399CysThr: 0.399 ± 0.184
0.239CysVal: 0.239 ± 0.179
0.159CysTrp: 0.159 ± 0.116
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.691AspAla: 8.691 ± 0.72
0.399AspCys: 0.399 ± 0.18
4.066AspAsp: 4.066 ± 0.454
2.551AspGlu: 2.551 ± 0.387
1.914AspPhe: 1.914 ± 0.359
5.262AspGly: 5.262 ± 0.544
1.196AspHis: 1.196 ± 0.313
1.993AspIle: 1.993 ± 0.341
2.472AspLys: 2.472 ± 0.537
6.697AspLeu: 6.697 ± 0.548
1.595AspMet: 1.595 ± 0.268
1.116AspAsn: 1.116 ± 0.321
3.588AspPro: 3.588 ± 0.572
2.153AspGln: 2.153 ± 0.567
4.545AspArg: 4.545 ± 0.742
2.232AspSer: 2.232 ± 0.475
2.551AspThr: 2.551 ± 0.426
4.146AspVal: 4.146 ± 0.668
1.276AspTrp: 1.276 ± 0.283
1.754AspTyr: 1.754 ± 0.386
0.0AspXaa: 0.0 ± 0.0
Glu
9.329GluAla: 9.329 ± 0.962
0.239GluCys: 0.239 ± 0.136
3.03GluAsp: 3.03 ± 0.685
2.87GluGlu: 2.87 ± 0.538
1.914GluPhe: 1.914 ± 0.428
3.668GluGly: 3.668 ± 0.589
1.116GluHis: 1.116 ± 0.347
5.262GluIle: 5.262 ± 0.864
2.631GluLys: 2.631 ± 0.448
5.262GluLeu: 5.262 ± 0.604
1.515GluMet: 1.515 ± 0.307
1.754GluAsn: 1.754 ± 0.335
2.153GluPro: 2.153 ± 0.478
2.392GluGln: 2.392 ± 0.428
4.306GluArg: 4.306 ± 0.586
1.515GluSer: 1.515 ± 0.346
4.066GluThr: 4.066 ± 0.498
3.987GluVal: 3.987 ± 0.563
1.116GluTrp: 1.116 ± 0.295
1.674GluTyr: 1.674 ± 0.365
0.0GluXaa: 0.0 ± 0.0
Phe
3.907PheAla: 3.907 ± 0.646
0.239PheCys: 0.239 ± 0.134
2.392PheAsp: 2.392 ± 0.476
2.073PheGlu: 2.073 ± 0.328
0.797PhePhe: 0.797 ± 0.227
3.428PheGly: 3.428 ± 0.643
0.399PheHis: 0.399 ± 0.176
0.877PheIle: 0.877 ± 0.26
1.116PheLys: 1.116 ± 0.283
2.472PheLeu: 2.472 ± 0.453
0.718PheMet: 0.718 ± 0.231
0.718PheAsn: 0.718 ± 0.206
1.595PhePro: 1.595 ± 0.385
1.116PheGln: 1.116 ± 0.332
1.754PheArg: 1.754 ± 0.318
1.754PheSer: 1.754 ± 0.389
1.754PheThr: 1.754 ± 0.284
2.711PheVal: 2.711 ± 0.518
0.877PheTrp: 0.877 ± 0.309
0.638PheTyr: 0.638 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
9.967GlyAla: 9.967 ± 0.964
0.718GlyCys: 0.718 ± 0.214
4.066GlyAsp: 4.066 ± 0.592
4.704GlyGlu: 4.704 ± 0.538
2.87GlyPhe: 2.87 ± 0.431
8.292GlyGly: 8.292 ± 1.201
0.957GlyHis: 0.957 ± 0.278
3.428GlyIle: 3.428 ± 0.508
5.183GlyLys: 5.183 ± 0.73
8.292GlyLeu: 8.292 ± 0.855
2.631GlyMet: 2.631 ± 0.428
1.595GlyAsn: 1.595 ± 0.423
2.711GlyPro: 2.711 ± 0.479
3.03GlyGln: 3.03 ± 0.575
5.82GlyArg: 5.82 ± 0.635
4.226GlySer: 4.226 ± 0.606
6.06GlyThr: 6.06 ± 0.926
6.139GlyVal: 6.139 ± 0.583
1.754GlyTrp: 1.754 ± 0.326
2.711GlyTyr: 2.711 ± 0.58
0.0GlyXaa: 0.0 ± 0.0
His
1.355HisAla: 1.355 ± 0.329
0.08HisCys: 0.08 ± 0.081
0.797HisAsp: 0.797 ± 0.242
1.116HisGlu: 1.116 ± 0.297
0.877HisPhe: 0.877 ± 0.293
1.834HisGly: 1.834 ± 0.334
0.478HisHis: 0.478 ± 0.164
1.276HisIle: 1.276 ± 0.348
0.638HisLys: 0.638 ± 0.17
0.957HisLeu: 0.957 ± 0.317
0.399HisMet: 0.399 ± 0.179
0.478HisAsn: 0.478 ± 0.173
1.355HisPro: 1.355 ± 0.425
0.718HisGln: 0.718 ± 0.2
1.355HisArg: 1.355 ± 0.35
0.797HisSer: 0.797 ± 0.243
0.558HisThr: 0.558 ± 0.208
1.355HisVal: 1.355 ± 0.32
0.239HisTrp: 0.239 ± 0.16
0.399HisTyr: 0.399 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
6.379IleAla: 6.379 ± 0.743
0.399IleCys: 0.399 ± 0.193
3.747IleAsp: 3.747 ± 0.555
5.82IleGlu: 5.82 ± 0.85
1.037IlePhe: 1.037 ± 0.281
3.588IleGly: 3.588 ± 0.498
0.478IleHis: 0.478 ± 0.185
1.595IleIle: 1.595 ± 0.322
1.435IleLys: 1.435 ± 0.317
3.269IleLeu: 3.269 ± 0.425
1.037IleMet: 1.037 ± 0.337
1.196IleAsn: 1.196 ± 0.353
2.073IlePro: 2.073 ± 0.343
1.276IleGln: 1.276 ± 0.31
3.668IleArg: 3.668 ± 0.56
3.588IleSer: 3.588 ± 0.487
3.747IleThr: 3.747 ± 0.453
3.987IleVal: 3.987 ± 0.757
1.276IleTrp: 1.276 ± 0.266
1.355IleTyr: 1.355 ± 0.241
0.0IleXaa: 0.0 ± 0.0
Lys
6.618LysAla: 6.618 ± 0.651
0.08LysCys: 0.08 ± 0.082
2.312LysAsp: 2.312 ± 0.33
1.834LysGlu: 1.834 ± 0.433
1.595LysPhe: 1.595 ± 0.341
3.508LysGly: 3.508 ± 0.543
0.558LysHis: 0.558 ± 0.258
2.232LysIle: 2.232 ± 0.427
2.073LysLys: 2.073 ± 0.423
3.508LysLeu: 3.508 ± 0.628
0.478LysMet: 0.478 ± 0.217
0.718LysAsn: 0.718 ± 0.244
2.551LysPro: 2.551 ± 0.492
1.116LysGln: 1.116 ± 0.323
3.428LysArg: 3.428 ± 0.591
1.834LysSer: 1.834 ± 0.337
2.073LysThr: 2.073 ± 0.476
3.03LysVal: 3.03 ± 0.458
0.558LysTrp: 0.558 ± 0.179
0.957LysTyr: 0.957 ± 0.211
0.0LysXaa: 0.0 ± 0.0
Leu
10.604LeuAla: 10.604 ± 0.746
0.558LeuCys: 0.558 ± 0.198
4.864LeuAsp: 4.864 ± 0.613
5.103LeuGlu: 5.103 ± 0.534
2.551LeuPhe: 2.551 ± 0.501
7.096LeuGly: 7.096 ± 0.663
1.037LeuHis: 1.037 ± 0.278
4.226LeuIle: 4.226 ± 0.562
3.269LeuLys: 3.269 ± 0.715
5.9LeuLeu: 5.9 ± 0.659
1.834LeuMet: 1.834 ± 0.421
2.232LeuAsn: 2.232 ± 0.446
5.183LeuPro: 5.183 ± 0.614
3.11LeuGln: 3.11 ± 0.542
6.618LeuArg: 6.618 ± 0.847
5.422LeuSer: 5.422 ± 0.777
6.06LeuThr: 6.06 ± 0.53
4.385LeuVal: 4.385 ± 0.523
2.392LeuTrp: 2.392 ± 0.632
1.355LeuTyr: 1.355 ± 0.386
0.0LeuXaa: 0.0 ± 0.0
Met
2.631MetAla: 2.631 ± 0.482
0.478MetCys: 0.478 ± 0.2
1.196MetAsp: 1.196 ± 0.402
1.037MetGlu: 1.037 ± 0.299
0.718MetPhe: 0.718 ± 0.218
2.153MetGly: 2.153 ± 0.467
0.239MetHis: 0.239 ± 0.147
0.877MetIle: 0.877 ± 0.286
0.718MetLys: 0.718 ± 0.225
2.392MetLeu: 2.392 ± 0.43
0.558MetMet: 0.558 ± 0.221
0.957MetAsn: 0.957 ± 0.235
1.276MetPro: 1.276 ± 0.35
1.037MetGln: 1.037 ± 0.274
1.595MetArg: 1.595 ± 0.347
1.754MetSer: 1.754 ± 0.354
3.03MetThr: 3.03 ± 0.544
1.276MetVal: 1.276 ± 0.387
0.478MetTrp: 0.478 ± 0.225
0.319MetTyr: 0.319 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
3.189AsnAla: 3.189 ± 0.454
0.159AsnCys: 0.159 ± 0.108
1.674AsnAsp: 1.674 ± 0.323
0.718AsnGlu: 0.718 ± 0.28
0.718AsnPhe: 0.718 ± 0.207
1.993AsnGly: 1.993 ± 0.401
0.159AsnHis: 0.159 ± 0.185
1.116AsnIle: 1.116 ± 0.289
0.638AsnLys: 0.638 ± 0.257
1.834AsnLeu: 1.834 ± 0.408
0.399AsnMet: 0.399 ± 0.187
0.399AsnAsn: 0.399 ± 0.162
2.073AsnPro: 2.073 ± 0.473
0.877AsnGln: 0.877 ± 0.304
1.834AsnArg: 1.834 ± 0.41
1.037AsnSer: 1.037 ± 0.24
1.355AsnThr: 1.355 ± 0.364
1.914AsnVal: 1.914 ± 0.396
0.239AsnTrp: 0.239 ± 0.179
0.478AsnTyr: 0.478 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
6.379ProAla: 6.379 ± 0.726
0.239ProCys: 0.239 ± 0.141
4.226ProAsp: 4.226 ± 0.789
2.95ProGlu: 2.95 ± 0.581
1.435ProPhe: 1.435 ± 0.36
4.226ProGly: 4.226 ± 0.677
1.196ProHis: 1.196 ± 0.254
2.87ProIle: 2.87 ± 0.487
2.312ProLys: 2.312 ± 0.516
3.11ProLeu: 3.11 ± 0.421
1.037ProMet: 1.037 ± 0.308
1.355ProAsn: 1.355 ± 0.377
2.232ProPro: 2.232 ± 0.325
1.595ProGln: 1.595 ± 0.329
2.791ProArg: 2.791 ± 0.499
2.631ProSer: 2.631 ± 0.498
2.711ProThr: 2.711 ± 0.401
5.183ProVal: 5.183 ± 0.646
0.718ProTrp: 0.718 ± 0.21
1.355ProTyr: 1.355 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
3.747GlnAla: 3.747 ± 0.677
0.159GlnCys: 0.159 ± 0.114
1.355GlnAsp: 1.355 ± 0.345
1.515GlnGlu: 1.515 ± 0.343
1.834GlnPhe: 1.834 ± 0.293
2.551GlnGly: 2.551 ± 0.474
1.116GlnHis: 1.116 ± 0.232
3.03GlnIle: 3.03 ± 0.446
1.435GlnLys: 1.435 ± 0.358
4.066GlnLeu: 4.066 ± 0.594
0.957GlnMet: 0.957 ± 0.302
0.877GlnAsn: 0.877 ± 0.245
2.551GlnPro: 2.551 ± 0.436
1.196GlnGln: 1.196 ± 0.3
1.754GlnArg: 1.754 ± 0.388
1.595GlnSer: 1.595 ± 0.324
2.232GlnThr: 2.232 ± 0.349
1.834GlnVal: 1.834 ± 0.425
0.638GlnTrp: 0.638 ± 0.175
0.877GlnTyr: 0.877 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
9.488ArgAla: 9.488 ± 0.818
0.399ArgCys: 0.399 ± 0.185
4.306ArgAsp: 4.306 ± 0.599
4.465ArgGlu: 4.465 ± 0.673
2.551ArgPhe: 2.551 ± 0.4
5.103ArgGly: 5.103 ± 0.684
1.834ArgHis: 1.834 ± 0.454
2.95ArgIle: 2.95 ± 0.516
3.11ArgLys: 3.11 ± 0.509
5.262ArgLeu: 5.262 ± 0.766
2.472ArgMet: 2.472 ± 0.465
1.834ArgAsn: 1.834 ± 0.403
3.907ArgPro: 3.907 ± 0.575
2.392ArgGln: 2.392 ± 0.48
5.98ArgArg: 5.98 ± 0.639
3.668ArgSer: 3.668 ± 0.531
3.03ArgThr: 3.03 ± 0.45
4.624ArgVal: 4.624 ± 0.628
1.355ArgTrp: 1.355 ± 0.299
1.515ArgTyr: 1.515 ± 0.311
0.0ArgXaa: 0.0 ± 0.0
Ser
5.9SerAla: 5.9 ± 0.646
0.478SerCys: 0.478 ± 0.189
2.87SerAsp: 2.87 ± 0.597
3.747SerGlu: 3.747 ± 0.597
1.834SerPhe: 1.834 ± 0.4
5.581SerGly: 5.581 ± 0.754
0.877SerHis: 0.877 ± 0.24
3.11SerIle: 3.11 ± 0.476
2.392SerLys: 2.392 ± 0.375
4.306SerLeu: 4.306 ± 0.549
1.595SerMet: 1.595 ± 0.295
1.355SerAsn: 1.355 ± 0.279
1.993SerPro: 1.993 ± 0.418
1.515SerGln: 1.515 ± 0.415
3.428SerArg: 3.428 ± 0.427
2.711SerSer: 2.711 ± 0.574
3.03SerThr: 3.03 ± 0.442
3.508SerVal: 3.508 ± 0.564
1.435SerTrp: 1.435 ± 0.313
1.754SerTyr: 1.754 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
7.814ThrAla: 7.814 ± 0.867
0.159ThrCys: 0.159 ± 0.109
3.588ThrAsp: 3.588 ± 0.48
3.668ThrGlu: 3.668 ± 0.561
1.914ThrPhe: 1.914 ± 0.348
6.139ThrGly: 6.139 ± 0.814
1.276ThrHis: 1.276 ± 0.338
4.465ThrIle: 4.465 ± 0.801
2.153ThrLys: 2.153 ± 0.428
5.103ThrLeu: 5.103 ± 0.528
1.037ThrMet: 1.037 ± 0.333
1.355ThrAsn: 1.355 ± 0.323
3.11ThrPro: 3.11 ± 0.521
2.312ThrGln: 2.312 ± 0.478
3.11ThrArg: 3.11 ± 0.48
3.428ThrSer: 3.428 ± 0.482
3.907ThrThr: 3.907 ± 0.602
4.226ThrVal: 4.226 ± 0.577
1.037ThrTrp: 1.037 ± 0.277
1.515ThrTyr: 1.515 ± 0.323
0.0ThrXaa: 0.0 ± 0.0
Val
10.285ValAla: 10.285 ± 1.262
0.718ValCys: 0.718 ± 0.21
3.827ValAsp: 3.827 ± 0.503
4.306ValGlu: 4.306 ± 0.604
1.754ValPhe: 1.754 ± 0.409
5.581ValGly: 5.581 ± 0.554
0.957ValHis: 0.957 ± 0.254
3.588ValIle: 3.588 ± 0.586
2.711ValLys: 2.711 ± 0.359
5.82ValLeu: 5.82 ± 0.661
1.435ValMet: 1.435 ± 0.353
1.196ValAsn: 1.196 ± 0.326
3.588ValPro: 3.588 ± 0.512
2.711ValGln: 2.711 ± 0.502
4.146ValArg: 4.146 ± 0.591
5.262ValSer: 5.262 ± 0.72
5.262ValThr: 5.262 ± 0.569
5.581ValVal: 5.581 ± 0.52
1.037ValTrp: 1.037 ± 0.27
0.638ValTyr: 0.638 ± 0.248
0.0ValXaa: 0.0 ± 0.0
Trp
1.993TrpAla: 1.993 ± 0.425
0.319TrpCys: 0.319 ± 0.126
1.515TrpAsp: 1.515 ± 0.455
0.638TrpGlu: 0.638 ± 0.25
0.558TrpPhe: 0.558 ± 0.202
2.073TrpGly: 2.073 ± 0.322
0.399TrpHis: 0.399 ± 0.314
0.877TrpIle: 0.877 ± 0.235
0.478TrpLys: 0.478 ± 0.215
1.435TrpLeu: 1.435 ± 0.39
0.399TrpMet: 0.399 ± 0.18
0.638TrpAsn: 0.638 ± 0.234
0.718TrpPro: 0.718 ± 0.199
1.116TrpGln: 1.116 ± 0.38
1.754TrpArg: 1.754 ± 0.331
1.355TrpSer: 1.355 ± 0.362
1.116TrpThr: 1.116 ± 0.279
1.037TrpVal: 1.037 ± 0.29
0.399TrpTrp: 0.399 ± 0.177
0.239TrpTyr: 0.239 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.472TyrAla: 2.472 ± 0.301
0.159TyrCys: 0.159 ± 0.116
1.674TyrAsp: 1.674 ± 0.256
1.276TyrGlu: 1.276 ± 0.284
0.877TyrPhe: 0.877 ± 0.299
2.073TyrGly: 2.073 ± 0.331
0.399TyrHis: 0.399 ± 0.26
0.877TyrIle: 0.877 ± 0.307
0.797TyrLys: 0.797 ± 0.269
1.754TyrLeu: 1.754 ± 0.37
0.797TyrMet: 0.797 ± 0.31
0.478TyrAsn: 0.478 ± 0.146
1.435TyrPro: 1.435 ± 0.395
0.478TyrGln: 0.478 ± 0.206
1.993TyrArg: 1.993 ± 0.385
0.718TyrSer: 0.718 ± 0.211
1.196TyrThr: 1.196 ± 0.334
1.196TyrVal: 1.196 ± 0.304
0.558TyrTrp: 0.558 ± 0.231
0.159TyrTyr: 0.159 ± 0.09
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12543 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski