Amino acid dipepetide frequency for Bacillus shackletonii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.136AlaAla: 5.136 ± 0.071	0.567AlaCys: 0.567 ± 0.023	3.042AlaAsp: 3.042 ± 0.048	4.143AlaGlu: 4.143 ± 0.069	3.207AlaPhe: 3.207 ± 0.057	4.828AlaGly: 4.828 ± 0.098	1.142AlaHis: 1.142 ± 0.028	5.991AlaIle: 5.991 ± 0.071	4.892AlaLys: 4.892 ± 0.063	6.681AlaLeu: 6.681 ± 0.075	1.905AlaMet: 1.905 ± 0.039	2.793AlaAsn: 2.793 ± 0.048	1.887AlaPro: 1.887 ± 0.038	1.825AlaGln: 1.825 ± 0.031	2.384AlaArg: 2.384 ± 0.046	3.824AlaSer: 3.824 ± 0.057	3.189AlaThr: 3.189 ± 0.063	4.685AlaVal: 4.685 ± 0.062	0.577AlaTrp: 0.577 ± 0.021	2.182AlaTyr: 2.182 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.446CysAla: 0.446 ± 0.018	0.1CysCys: 0.1 ± 0.009	0.411CysAsp: 0.411 ± 0.017	0.496CysGlu: 0.496 ± 0.02	0.389CysPhe: 0.389 ± 0.017	0.684CysGly: 0.684 ± 0.025	0.215CysHis: 0.215 ± 0.012	0.641CysIle: 0.641 ± 0.024	0.406CysLys: 0.406 ± 0.018	0.808CysLeu: 0.808 ± 0.023	0.197CysMet: 0.197 ± 0.013	0.314CysAsn: 0.314 ± 0.016	0.357CysPro: 0.357 ± 0.018	0.24CysGln: 0.24 ± 0.014	0.29CysArg: 0.29 ± 0.015	0.528CysSer: 0.528 ± 0.017	0.418CysThr: 0.418 ± 0.018	0.438CysVal: 0.438 ± 0.021	0.075CysTrp: 0.075 ± 0.007	0.3CysTyr: 0.3 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	2.967AspAla: 2.967 ± 0.051	0.4AspCys: 0.4 ± 0.02	2.231AspAsp: 2.231 ± 0.041	3.895AspGlu: 3.895 ± 0.052	2.507AspPhe: 2.507 ± 0.045	3.308AspGly: 3.308 ± 0.054	1.126AspHis: 1.126 ± 0.027	4.37AspIle: 4.37 ± 0.069	3.352AspLys: 3.352 ± 0.06	4.814AspLeu: 4.814 ± 0.063	1.262AspMet: 1.262 ± 0.03	1.988AspAsn: 1.988 ± 0.038	1.966AspPro: 1.966 ± 0.035	1.879AspGln: 1.879 ± 0.038	2.053AspArg: 2.053 ± 0.04	2.623AspSer: 2.623 ± 0.048	2.291AspThr: 2.291 ± 0.04	3.5AspVal: 3.5 ± 0.055	0.607AspTrp: 0.607 ± 0.023	2.174AspTyr: 2.174 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	4.501GluAla: 4.501 ± 0.067	0.45GluCys: 0.45 ± 0.022	3.435GluAsp: 3.435 ± 0.062	6.18GluGlu: 6.18 ± 0.085	2.607GluPhe: 2.607 ± 0.045	3.869GluGly: 3.869 ± 0.06	1.432GluHis: 1.432 ± 0.036	5.889GluIle: 5.889 ± 0.061	6.847GluLys: 6.847 ± 0.081	6.718GluLeu: 6.718 ± 0.081	2.288GluMet: 2.288 ± 0.038	3.924GluAsn: 3.924 ± 0.056	1.814GluPro: 1.814 ± 0.039	2.844GluGln: 2.844 ± 0.053	3.132GluArg: 3.132 ± 0.052	3.423GluSer: 3.423 ± 0.047	3.466GluThr: 3.466 ± 0.053	4.555GluVal: 4.555 ± 0.072	0.849GluTrp: 0.849 ± 0.026	2.374GluTyr: 2.374 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.022PheAla: 3.022 ± 0.056	0.394PheCys: 0.394 ± 0.018	2.357PheAsp: 2.357 ± 0.046	2.726PheGlu: 2.726 ± 0.048	2.438PhePhe: 2.438 ± 0.058	3.382PheGly: 3.382 ± 0.051	1.205PheHis: 1.205 ± 0.029	4.422PheIle: 4.422 ± 0.081	2.614PheLys: 2.614 ± 0.052	4.8PheLeu: 4.8 ± 0.079	1.203PheMet: 1.203 ± 0.033	2.024PheAsn: 2.024 ± 0.039	1.756PhePro: 1.756 ± 0.037	1.794PheGln: 1.794 ± 0.036	1.535PheArg: 1.535 ± 0.033	3.404PheSer: 3.404 ± 0.058	2.625PheThr: 2.625 ± 0.049	3.099PheVal: 3.099 ± 0.061	0.499PheTrp: 0.499 ± 0.019	1.896PheTyr: 1.896 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.428GlyAla: 4.428 ± 0.094	0.659GlyCys: 0.659 ± 0.022	3.043GlyAsp: 3.043 ± 0.054	4.14GlyGlu: 4.14 ± 0.067	3.45GlyPhe: 3.45 ± 0.051	4.641GlyGly: 4.641 ± 0.072	1.33GlyHis: 1.33 ± 0.032	6.443GlyIle: 6.443 ± 0.079	5.493GlyLys: 5.493 ± 0.069	6.357GlyLeu: 6.357 ± 0.073	2.086GlyMet: 2.086 ± 0.04	2.998GlyAsn: 2.998 ± 0.053	1.734GlyPro: 1.734 ± 0.066	2.023GlyGln: 2.023 ± 0.041	2.425GlyArg: 2.425 ± 0.041	3.886GlySer: 3.886 ± 0.055	3.864GlyThr: 3.864 ± 0.058	4.912GlyVal: 4.912 ± 0.063	0.834GlyTrp: 0.834 ± 0.023	2.869GlyTyr: 2.869 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.257HisAla: 1.257 ± 0.03	0.215HisCys: 0.215 ± 0.013	1.054HisAsp: 1.054 ± 0.029	1.342HisGlu: 1.342 ± 0.031	1.227HisPhe: 1.227 ± 0.027	1.388HisGly: 1.388 ± 0.031	0.769HisHis: 0.769 ± 0.026	1.664HisIle: 1.664 ± 0.032	1.1HisLys: 1.1 ± 0.031	2.24HisLeu: 2.24 ± 0.046	0.491HisMet: 0.491 ± 0.019	0.834HisAsn: 0.834 ± 0.024	1.212HisPro: 1.212 ± 0.031	0.897HisGln: 0.897 ± 0.026	0.868HisArg: 0.868 ± 0.024	1.346HisSer: 1.346 ± 0.029	1.006HisThr: 1.006 ± 0.029	1.377HisVal: 1.377 ± 0.035	0.252HisTrp: 0.252 ± 0.014	0.954HisTyr: 0.954 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.006IleAla: 6.006 ± 0.071	0.774IleCys: 0.774 ± 0.024	4.307IleAsp: 4.307 ± 0.051	5.671IleGlu: 5.671 ± 0.068	3.869IlePhe: 3.869 ± 0.066	6.286IleGly: 6.286 ± 0.089	1.928IleHis: 1.928 ± 0.036	7.148IleIle: 7.148 ± 0.094	5.3IleLys: 5.3 ± 0.067	8.095IleLeu: 8.095 ± 0.092	2.084IleMet: 2.084 ± 0.041	3.669IleAsn: 3.669 ± 0.057	3.728IlePro: 3.728 ± 0.057	3.286IleGln: 3.286 ± 0.055	3.318IleArg: 3.318 ± 0.047	5.762IleSer: 5.762 ± 0.072	4.605IleThr: 4.605 ± 0.06	5.822IleVal: 5.822 ± 0.074	0.73IleTrp: 0.73 ± 0.025	2.768IleTyr: 2.768 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.696LysAla: 4.696 ± 0.063	0.396LysCys: 0.396 ± 0.016	4.086LysAsp: 4.086 ± 0.058	6.924LysGlu: 6.924 ± 0.082	2.169LysPhe: 2.169 ± 0.045	4.769LysGly: 4.769 ± 0.061	1.461LysHis: 1.461 ± 0.037	5.515LysIle: 5.515 ± 0.055	6.566LysLys: 6.566 ± 0.083	6.341LysLeu: 6.341 ± 0.075	2.491LysMet: 2.491 ± 0.04	4.058LysAsn: 4.058 ± 0.057	2.358LysPro: 2.358 ± 0.046	3.184LysGln: 3.184 ± 0.051	3.317LysArg: 3.317 ± 0.057	3.951LysSer: 3.951 ± 0.055	3.873LysThr: 3.873 ± 0.058	4.928LysVal: 4.928 ± 0.062	0.958LysTrp: 0.958 ± 0.028	2.599LysTyr: 2.599 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	6.683LeuAla: 6.683 ± 0.083	0.755LeuCys: 0.755 ± 0.027	4.665LeuAsp: 4.665 ± 0.065	6.468LeuGlu: 6.468 ± 0.074	4.866LeuPhe: 4.866 ± 0.079	6.388LeuGly: 6.388 ± 0.073	2.058LeuHis: 2.058 ± 0.033	7.449LeuIle: 7.449 ± 0.1	7.226LeuLys: 7.226 ± 0.068	10.015LeuLeu: 10.015 ± 0.096	2.555LeuMet: 2.555 ± 0.04	4.602LeuAsn: 4.602 ± 0.055	3.918LeuPro: 3.918 ± 0.057	3.69LeuGln: 3.69 ± 0.053	3.452LeuArg: 3.452 ± 0.053	6.982LeuSer: 6.982 ± 0.078	5.472LeuThr: 5.472 ± 0.064	6.043LeuVal: 6.043 ± 0.062	0.835LeuTrp: 0.835 ± 0.026	3.199LeuTyr: 3.199 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	1.884MetAla: 1.884 ± 0.039	0.163MetCys: 0.163 ± 0.01	1.546MetAsp: 1.546 ± 0.034	2.273MetGlu: 2.273 ± 0.042	1.061MetPhe: 1.061 ± 0.028	1.825MetGly: 1.825 ± 0.038	0.42MetHis: 0.42 ± 0.018	2.2MetIle: 2.2 ± 0.047	2.597MetLys: 2.597 ± 0.041	2.436MetLeu: 2.436 ± 0.045	0.856MetMet: 0.856 ± 0.026	1.649MetAsn: 1.649 ± 0.033	0.946MetPro: 0.946 ± 0.028	0.831MetGln: 0.831 ± 0.026	1.065MetArg: 1.065 ± 0.026	1.634MetSer: 1.634 ± 0.034	1.517MetThr: 1.517 ± 0.038	1.849MetVal: 1.849 ± 0.036	0.205MetTrp: 0.205 ± 0.012	0.749MetTyr: 0.749 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.932AsnAla: 2.932 ± 0.051	0.32AsnCys: 0.32 ± 0.018	2.355AsnAsp: 2.355 ± 0.044	3.607AsnGlu: 3.607 ± 0.057	1.798AsnPhe: 1.798 ± 0.035	3.707AsnGly: 3.707 ± 0.063	1.214AsnHis: 1.214 ± 0.031	4.034AsnIle: 4.034 ± 0.063	3.366AsnLys: 3.366 ± 0.053	4.154AsnLeu: 4.154 ± 0.06	1.253AsnMet: 1.253 ± 0.03	2.325AsnAsn: 2.325 ± 0.051	2.308AsnPro: 2.308 ± 0.04	2.216AsnGln: 2.216 ± 0.046	2.099AsnArg: 2.099 ± 0.045	2.533AsnSer: 2.533 ± 0.042	2.299AsnThr: 2.299 ± 0.034	3.162AsnVal: 3.162 ± 0.047	0.584AsnTrp: 0.584 ± 0.021	1.594AsnTyr: 1.594 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	2.157ProAla: 2.157 ± 0.07	0.23ProCys: 0.23 ± 0.015	1.94ProAsp: 1.94 ± 0.042	2.705ProGlu: 2.705 ± 0.047	2.088ProPhe: 2.088 ± 0.04	2.177ProGly: 2.177 ± 0.044	0.821ProHis: 0.821 ± 0.026	3.178ProIle: 3.178 ± 0.053	2.465ProLys: 2.465 ± 0.045	3.53ProLeu: 3.53 ± 0.053	0.846ProMet: 0.846 ± 0.026	1.923ProAsn: 1.923 ± 0.038	1.006ProPro: 1.006 ± 0.03	1.096ProGln: 1.096 ± 0.027	1.057ProArg: 1.057 ± 0.024	2.358ProSer: 2.358 ± 0.036	1.978ProThr: 1.978 ± 0.037	2.637ProVal: 2.637 ± 0.047	0.394ProTrp: 0.394 ± 0.018	1.483ProTyr: 1.483 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.355GlnAla: 2.355 ± 0.043	0.219GlnCys: 0.219 ± 0.013	1.649GlnAsp: 1.649 ± 0.037	2.647GlnGlu: 2.647 ± 0.055	1.741GlnPhe: 1.741 ± 0.037	2.007GlnGly: 2.007 ± 0.043	0.82GlnHis: 0.82 ± 0.025	2.787GlnIle: 2.787 ± 0.046	2.967GlnLys: 2.967 ± 0.047	3.836GlnLeu: 3.836 ± 0.054	1.101GlnMet: 1.101 ± 0.03	1.9GlnAsn: 1.9 ± 0.038	1.149GlnPro: 1.149 ± 0.028	1.613GlnGln: 1.613 ± 0.038	1.324GlnArg: 1.324 ± 0.029	2.158GlnSer: 2.158 ± 0.04	1.875GlnThr: 1.875 ± 0.034	2.239GlnVal: 2.239 ± 0.04	0.387GlnTrp: 0.387 ± 0.017	1.483GlnTyr: 1.483 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.089ArgAla: 2.089 ± 0.042	0.276ArgCys: 0.276 ± 0.014	1.885ArgAsp: 1.885 ± 0.038	2.921ArgGlu: 2.921 ± 0.048	1.902ArgPhe: 1.902 ± 0.039	2.206ArgGly: 2.206 ± 0.045	0.741ArgHis: 0.741 ± 0.023	3.21ArgIle: 3.21 ± 0.052	3.375ArgLys: 3.375 ± 0.052	3.794ArgLeu: 3.794 ± 0.047	1.205ArgMet: 1.205 ± 0.029	2.121ArgAsn: 2.121 ± 0.045	1.213ArgPro: 1.213 ± 0.033	1.361ArgGln: 1.361 ± 0.031	1.698ArgArg: 1.698 ± 0.043	2.089ArgSer: 2.089 ± 0.044	1.992ArgThr: 1.992 ± 0.038	2.423ArgVal: 2.423 ± 0.047	0.402ArgTrp: 0.402 ± 0.018	1.486ArgTyr: 1.486 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.697SerAla: 3.697 ± 0.054	0.454SerCys: 0.454 ± 0.017	2.811SerAsp: 2.811 ± 0.042	3.702SerGlu: 3.702 ± 0.048	3.435SerPhe: 3.435 ± 0.057	4.433SerGly: 4.433 ± 0.061	1.206SerHis: 1.206 ± 0.027	5.805SerIle: 5.805 ± 0.071	4.287SerLys: 4.287 ± 0.066	6.266SerLeu: 6.266 ± 0.077	1.652SerMet: 1.652 ± 0.034	2.912SerAsn: 2.912 ± 0.05	2.189SerPro: 2.189 ± 0.039	1.952SerGln: 1.952 ± 0.04	2.209SerArg: 2.209 ± 0.043	4.063SerSer: 4.063 ± 0.069	3.039SerThr: 3.039 ± 0.05	4.063SerVal: 4.063 ± 0.06	0.666SerTrp: 0.666 ± 0.027	2.308SerTyr: 2.308 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	3.562ThrAla: 3.562 ± 0.051	0.361ThrCys: 0.361 ± 0.018	2.517ThrAsp: 2.517 ± 0.042	3.146ThrGlu: 3.146 ± 0.058	2.719ThrPhe: 2.719 ± 0.044	4.06ThrGly: 4.06 ± 0.09	1.052ThrHis: 1.052 ± 0.026	4.942ThrIle: 4.942 ± 0.069	3.572ThrLys: 3.572 ± 0.06	5.048ThrLeu: 5.048 ± 0.064	1.192ThrMet: 1.192 ± 0.029	2.552ThrAsn: 2.552 ± 0.041	2.163ThrPro: 2.163 ± 0.04	1.362ThrGln: 1.362 ± 0.032	1.707ThrArg: 1.707 ± 0.037	3.282ThrSer: 3.282 ± 0.05	2.745ThrThr: 2.745 ± 0.056	3.907ThrVal: 3.907 ± 0.054	0.563ThrTrp: 0.563 ± 0.021	1.884ThrTyr: 1.884 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	4.447ValAla: 4.447 ± 0.067	0.562ValCys: 0.562 ± 0.02	3.472ValAsp: 3.472 ± 0.056	4.509ValGlu: 4.509 ± 0.06	3.179ValPhe: 3.179 ± 0.051	4.478ValGly: 4.478 ± 0.06	1.375ValHis: 1.375 ± 0.033	5.706ValIle: 5.706 ± 0.063	4.978ValLys: 4.978 ± 0.067	6.595ValLeu: 6.595 ± 0.065	1.839ValMet: 1.839 ± 0.04	3.132ValAsn: 3.132 ± 0.049	2.591ValPro: 2.591 ± 0.045	2.229ValGln: 2.229 ± 0.042	2.439ValArg: 2.439 ± 0.042	4.393ValSer: 4.393 ± 0.067	3.784ValThr: 3.784 ± 0.062	4.674ValVal: 4.674 ± 0.067	0.633ValTrp: 0.633 ± 0.02	2.192ValTyr: 2.192 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.615TrpAla: 0.615 ± 0.023	0.111TrpCys: 0.111 ± 0.01	0.532TrpAsp: 0.532 ± 0.021	0.633TrpGlu: 0.633 ± 0.023	0.547TrpPhe: 0.547 ± 0.023	0.709TrpGly: 0.709 ± 0.023	0.199TrpHis: 0.199 ± 0.011	0.939TrpIle: 0.939 ± 0.026	0.855TrpLys: 0.855 ± 0.027	1.12TrpLeu: 1.12 ± 0.028	0.306TrpMet: 0.306 ± 0.015	0.635TrpAsn: 0.635 ± 0.025	0.264TrpPro: 0.264 ± 0.016	0.359TrpGln: 0.359 ± 0.015	0.418TrpArg: 0.418 ± 0.018	0.651TrpSer: 0.651 ± 0.021	0.52TrpThr: 0.52 ± 0.019	0.7TrpVal: 0.7 ± 0.021	0.16TrpTrp: 0.16 ± 0.011	0.38TrpTyr: 0.38 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.998TyrAla: 1.998 ± 0.039	0.348TyrCys: 0.348 ± 0.016	1.974TyrAsp: 1.974 ± 0.044	2.471TyrGlu: 2.471 ± 0.045	1.998TyrPhe: 1.998 ± 0.042	2.47TyrGly: 2.47 ± 0.049	0.977TyrHis: 0.977 ± 0.027	2.823TyrIle: 2.823 ± 0.039	2.381TyrLys: 2.381 ± 0.042	3.676TyrLeu: 3.676 ± 0.054	0.892TyrMet: 0.892 ± 0.025	1.509TyrAsn: 1.509 ± 0.036	1.509TyrPro: 1.509 ± 0.031	1.574TyrGln: 1.574 ± 0.034	1.598TyrArg: 1.598 ± 0.039	2.257TyrSer: 2.257 ± 0.04	1.779TyrThr: 1.779 ± 0.034	2.195TyrVal: 2.195 ± 0.038	0.444TyrTrp: 0.444 ± 0.017	1.543TyrTyr: 1.543 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4690 proteins (1351557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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