Amino acid dipepetide frequency for Streptomyces phage Kardashian

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.38AlaAla: 10.38 ± 1.935
0.522AlaCys: 0.522 ± 0.181
4.755AlaAsp: 4.755 ± 0.569
7.596AlaGlu: 7.596 ± 0.706
2.378AlaPhe: 2.378 ± 0.371
6.901AlaGly: 6.901 ± 1.642
1.044AlaHis: 1.044 ± 0.282
5.973AlaIle: 5.973 ± 0.7
7.19AlaLys: 7.19 ± 0.997
7.306AlaLeu: 7.306 ± 1.012
3.131AlaMet: 3.131 ± 0.39
3.305AlaAsn: 3.305 ± 0.465
2.783AlaPro: 2.783 ± 0.375
3.305AlaGln: 3.305 ± 0.562
4.523AlaArg: 4.523 ± 0.525
4.117AlaSer: 4.117 ± 0.628
5.393AlaThr: 5.393 ± 0.714
6.031AlaVal: 6.031 ± 0.749
0.986AlaTrp: 0.986 ± 0.277
2.957AlaTyr: 2.957 ± 0.458
0.0AlaXaa: 0.0 ± 0.0
Cys
0.464CysAla: 0.464 ± 0.196
0.232CysCys: 0.232 ± 0.159
0.232CysAsp: 0.232 ± 0.122
0.348CysGlu: 0.348 ± 0.158
0.116CysPhe: 0.116 ± 0.087
0.58CysGly: 0.58 ± 0.273
0.0CysHis: 0.0 ± 0.0
0.29CysIle: 0.29 ± 0.119
0.174CysLys: 0.174 ± 0.097
0.696CysLeu: 0.696 ± 0.189
0.174CysMet: 0.174 ± 0.116
0.174CysAsn: 0.174 ± 0.1
0.058CysPro: 0.058 ± 0.065
0.058CysGln: 0.058 ± 0.063
0.174CysArg: 0.174 ± 0.107
0.406CysSer: 0.406 ± 0.154
0.464CysThr: 0.464 ± 0.171
0.406CysVal: 0.406 ± 0.157
0.116CysTrp: 0.116 ± 0.08
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.857AspAla: 5.857 ± 0.732
0.406AspCys: 0.406 ± 0.155
3.711AspAsp: 3.711 ± 0.644
6.321AspGlu: 6.321 ± 0.816
2.899AspPhe: 2.899 ± 0.517
5.973AspGly: 5.973 ± 0.767
0.928AspHis: 0.928 ± 0.279
2.899AspIle: 2.899 ± 0.532
2.493AspLys: 2.493 ± 0.325
4.697AspLeu: 4.697 ± 0.402
1.74AspMet: 1.74 ± 0.312
2.204AspAsn: 2.204 ± 0.386
3.943AspPro: 3.943 ± 0.696
2.088AspGln: 2.088 ± 0.362
3.653AspArg: 3.653 ± 0.668
2.841AspSer: 2.841 ± 0.494
3.479AspThr: 3.479 ± 0.466
4.175AspVal: 4.175 ± 0.544
0.87AspTrp: 0.87 ± 0.24
1.798AspTyr: 1.798 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
5.335GluAla: 5.335 ± 0.738
0.116GluCys: 0.116 ± 0.089
5.277GluAsp: 5.277 ± 0.857
6.147GluGlu: 6.147 ± 0.951
3.247GluPhe: 3.247 ± 0.436
5.219GluGly: 5.219 ± 0.525
1.682GluHis: 1.682 ± 0.366
3.827GluIle: 3.827 ± 0.442
4.001GluLys: 4.001 ± 0.715
6.321GluLeu: 6.321 ± 0.703
2.609GluMet: 2.609 ± 0.326
2.32GluAsn: 2.32 ± 0.359
2.899GluPro: 2.899 ± 0.485
2.493GluGln: 2.493 ± 0.38
3.943GluArg: 3.943 ± 0.549
3.421GluSer: 3.421 ± 0.515
4.117GluThr: 4.117 ± 0.466
4.233GluVal: 4.233 ± 0.529
0.928GluTrp: 0.928 ± 0.216
2.435GluTyr: 2.435 ± 0.359
0.0GluXaa: 0.0 ± 0.0
Phe
3.421PheAla: 3.421 ± 0.55
0.232PheCys: 0.232 ± 0.149
2.783PheAsp: 2.783 ± 0.313
2.551PheGlu: 2.551 ± 0.407
1.334PhePhe: 1.334 ± 0.251
2.667PheGly: 2.667 ± 0.392
0.464PheHis: 0.464 ± 0.168
1.624PheIle: 1.624 ± 0.335
2.32PheLys: 2.32 ± 0.386
2.435PheLeu: 2.435 ± 0.359
0.58PheMet: 0.58 ± 0.171
1.856PheAsn: 1.856 ± 0.24
1.218PhePro: 1.218 ± 0.214
1.276PheGln: 1.276 ± 0.298
1.914PheArg: 1.914 ± 0.313
2.493PheSer: 2.493 ± 0.392
2.551PheThr: 2.551 ± 0.566
2.146PheVal: 2.146 ± 0.462
0.638PheTrp: 0.638 ± 0.259
1.392PheTyr: 1.392 ± 0.331
0.0PheXaa: 0.0 ± 0.0
Gly
6.611GlyAla: 6.611 ± 0.824
0.58GlyCys: 0.58 ± 0.27
5.219GlyAsp: 5.219 ± 0.598
4.581GlyGlu: 4.581 ± 0.396
3.189GlyPhe: 3.189 ± 0.52
4.523GlyGly: 4.523 ± 0.589
1.16GlyHis: 1.16 ± 0.224
4.581GlyIle: 4.581 ± 0.841
3.885GlyLys: 3.885 ± 0.695
5.625GlyLeu: 5.625 ± 1.314
2.551GlyMet: 2.551 ± 0.406
2.493GlyAsn: 2.493 ± 0.342
2.435GlyPro: 2.435 ± 0.376
1.798GlyGln: 1.798 ± 0.377
3.015GlyArg: 3.015 ± 0.338
4.871GlySer: 4.871 ± 0.604
6.147GlyThr: 6.147 ± 0.668
5.741GlyVal: 5.741 ± 0.827
1.624GlyTrp: 1.624 ± 0.341
3.595GlyTyr: 3.595 ± 0.488
0.0GlyXaa: 0.0 ± 0.0
His
1.218HisAla: 1.218 ± 0.331
0.174HisCys: 0.174 ± 0.106
0.986HisAsp: 0.986 ± 0.223
1.392HisGlu: 1.392 ± 0.321
0.464HisPhe: 0.464 ± 0.146
1.392HisGly: 1.392 ± 0.325
0.522HisHis: 0.522 ± 0.172
1.102HisIle: 1.102 ± 0.294
0.406HisLys: 0.406 ± 0.158
1.16HisLeu: 1.16 ± 0.322
0.522HisMet: 0.522 ± 0.138
0.696HisAsn: 0.696 ± 0.189
1.102HisPro: 1.102 ± 0.229
0.58HisGln: 0.58 ± 0.194
1.102HisArg: 1.102 ± 0.304
1.16HisSer: 1.16 ± 0.243
0.522HisThr: 0.522 ± 0.15
1.16HisVal: 1.16 ± 0.316
0.29HisTrp: 0.29 ± 0.137
1.566HisTyr: 1.566 ± 0.273
0.0HisXaa: 0.0 ± 0.0
Ile
4.349IleAla: 4.349 ± 0.532
0.232IleCys: 0.232 ± 0.109
4.117IleAsp: 4.117 ± 0.491
4.407IleGlu: 4.407 ± 0.575
2.32IlePhe: 2.32 ± 0.438
3.711IleGly: 3.711 ± 0.677
1.102IleHis: 1.102 ± 0.318
2.088IleIle: 2.088 ± 0.351
4.175IleLys: 4.175 ± 0.567
4.059IleLeu: 4.059 ± 0.518
1.16IleMet: 1.16 ± 0.323
1.914IleAsn: 1.914 ± 0.311
2.32IlePro: 2.32 ± 0.329
1.624IleGln: 1.624 ± 0.294
1.972IleArg: 1.972 ± 0.29
3.653IleSer: 3.653 ± 0.482
3.769IleThr: 3.769 ± 0.515
3.711IleVal: 3.711 ± 0.434
0.464IleTrp: 0.464 ± 0.168
1.392IleTyr: 1.392 ± 0.246
0.0IleXaa: 0.0 ± 0.0
Lys
6.379LysAla: 6.379 ± 0.667
0.348LysCys: 0.348 ± 0.148
3.073LysAsp: 3.073 ± 0.502
3.421LysGlu: 3.421 ± 0.506
1.972LysPhe: 1.972 ± 0.3
4.639LysGly: 4.639 ± 0.784
1.392LysHis: 1.392 ± 0.354
3.421LysIle: 3.421 ± 0.389
6.553LysLys: 6.553 ± 0.936
6.379LysLeu: 6.379 ± 0.736
2.262LysMet: 2.262 ± 0.39
2.378LysAsn: 2.378 ± 0.495
2.841LysPro: 2.841 ± 0.422
2.667LysGln: 2.667 ± 0.36
3.479LysArg: 3.479 ± 0.692
4.175LysSer: 4.175 ± 0.559
4.407LysThr: 4.407 ± 0.59
4.639LysVal: 4.639 ± 0.563
0.754LysTrp: 0.754 ± 0.209
1.972LysTyr: 1.972 ± 0.383
0.0LysXaa: 0.0 ± 0.0
Leu
8.988LeuAla: 8.988 ± 1.572
0.348LeuCys: 0.348 ± 0.137
4.697LeuAsp: 4.697 ± 0.554
4.233LeuGlu: 4.233 ± 0.603
2.609LeuPhe: 2.609 ± 0.43
6.031LeuGly: 6.031 ± 1.143
1.334LeuHis: 1.334 ± 0.288
4.233LeuIle: 4.233 ± 0.532
5.799LeuLys: 5.799 ± 0.725
6.147LeuLeu: 6.147 ± 0.764
1.856LeuMet: 1.856 ± 0.384
4.523LeuAsn: 4.523 ± 0.57
2.725LeuPro: 2.725 ± 0.398
2.667LeuGln: 2.667 ± 0.384
4.871LeuArg: 4.871 ± 0.69
4.987LeuSer: 4.987 ± 0.586
5.567LeuThr: 5.567 ± 0.641
5.625LeuVal: 5.625 ± 0.564
0.812LeuTrp: 0.812 ± 0.227
2.841LeuTyr: 2.841 ± 0.467
0.0LeuXaa: 0.0 ± 0.0
Met
2.725MetAla: 2.725 ± 0.429
0.116MetCys: 0.116 ± 0.077
1.566MetAsp: 1.566 ± 0.257
1.566MetGlu: 1.566 ± 0.399
0.58MetPhe: 0.58 ± 0.198
2.088MetGly: 2.088 ± 0.369
0.406MetHis: 0.406 ± 0.137
1.16MetIle: 1.16 ± 0.242
1.624MetLys: 1.624 ± 0.252
2.088MetLeu: 2.088 ± 0.38
0.638MetMet: 0.638 ± 0.197
1.044MetAsn: 1.044 ± 0.252
1.102MetPro: 1.102 ± 0.235
0.638MetGln: 0.638 ± 0.197
1.682MetArg: 1.682 ± 0.346
2.609MetSer: 2.609 ± 0.327
1.798MetThr: 1.798 ± 0.314
2.03MetVal: 2.03 ± 0.305
0.29MetTrp: 0.29 ± 0.14
0.638MetTyr: 0.638 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.247AsnAla: 3.247 ± 0.374
0.058AsnCys: 0.058 ± 0.061
3.015AsnAsp: 3.015 ± 0.446
2.435AsnGlu: 2.435 ± 0.398
1.102AsnPhe: 1.102 ± 0.267
3.479AsnGly: 3.479 ± 0.477
0.638AsnHis: 0.638 ± 0.161
1.914AsnIle: 1.914 ± 0.353
2.493AsnLys: 2.493 ± 0.321
3.073AsnLeu: 3.073 ± 0.433
0.87AsnMet: 0.87 ± 0.199
1.566AsnAsn: 1.566 ± 0.277
2.551AsnPro: 2.551 ± 0.37
2.262AsnGln: 2.262 ± 0.38
2.667AsnArg: 2.667 ± 0.568
2.841AsnSer: 2.841 ± 0.458
2.378AsnThr: 2.378 ± 0.442
2.957AsnVal: 2.957 ± 0.443
0.58AsnTrp: 0.58 ± 0.225
1.102AsnTyr: 1.102 ± 0.221
0.0AsnXaa: 0.0 ± 0.0
Pro
3.189ProAla: 3.189 ± 0.476
0.232ProCys: 0.232 ± 0.123
3.015ProAsp: 3.015 ± 0.53
3.421ProGlu: 3.421 ± 0.579
1.218ProPhe: 1.218 ± 0.219
3.015ProGly: 3.015 ± 0.456
0.812ProHis: 0.812 ± 0.28
2.03ProIle: 2.03 ± 0.316
3.015ProLys: 3.015 ± 0.464
2.667ProLeu: 2.667 ± 0.451
0.754ProMet: 0.754 ± 0.252
1.566ProAsn: 1.566 ± 0.283
1.566ProPro: 1.566 ± 0.353
1.16ProGln: 1.16 ± 0.246
1.856ProArg: 1.856 ± 0.38
3.537ProSer: 3.537 ± 0.537
2.899ProThr: 2.899 ± 0.484
2.378ProVal: 2.378 ± 0.349
0.348ProTrp: 0.348 ± 0.139
1.45ProTyr: 1.45 ± 0.328
0.0ProXaa: 0.0 ± 0.0
Gln
2.493GlnAla: 2.493 ± 0.435
0.29GlnCys: 0.29 ± 0.114
1.856GlnAsp: 1.856 ± 0.262
1.74GlnGlu: 1.74 ± 0.295
1.624GlnPhe: 1.624 ± 0.298
2.841GlnGly: 2.841 ± 0.405
0.87GlnHis: 0.87 ± 0.317
2.32GlnIle: 2.32 ± 0.393
2.551GlnLys: 2.551 ± 0.381
2.841GlnLeu: 2.841 ± 0.534
0.638GlnMet: 0.638 ± 0.184
1.45GlnAsn: 1.45 ± 0.303
0.754GlnPro: 0.754 ± 0.173
1.508GlnGln: 1.508 ± 0.239
1.856GlnArg: 1.856 ± 0.378
1.566GlnSer: 1.566 ± 0.334
2.32GlnThr: 2.32 ± 0.399
2.667GlnVal: 2.667 ± 0.372
0.348GlnTrp: 0.348 ± 0.16
1.102GlnTyr: 1.102 ± 0.18
0.0GlnXaa: 0.0 ± 0.0
Arg
3.769ArgAla: 3.769 ± 0.522
0.174ArgCys: 0.174 ± 0.12
2.957ArgAsp: 2.957 ± 0.439
4.001ArgGlu: 4.001 ± 0.426
1.624ArgPhe: 1.624 ± 0.337
2.667ArgGly: 2.667 ± 0.515
0.638ArgHis: 0.638 ± 0.232
3.131ArgIle: 3.131 ± 0.519
4.871ArgLys: 4.871 ± 0.773
5.103ArgLeu: 5.103 ± 0.559
1.16ArgMet: 1.16 ± 0.262
3.015ArgAsn: 3.015 ± 0.411
2.146ArgPro: 2.146 ± 0.486
1.624ArgGln: 1.624 ± 0.36
4.639ArgArg: 4.639 ± 0.755
2.667ArgSer: 2.667 ± 0.409
3.885ArgThr: 3.885 ± 0.573
3.769ArgVal: 3.769 ± 0.736
0.928ArgTrp: 0.928 ± 0.279
1.392ArgTyr: 1.392 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
5.973SerAla: 5.973 ± 0.729
0.29SerCys: 0.29 ± 0.148
3.537SerAsp: 3.537 ± 0.561
4.407SerGlu: 4.407 ± 0.611
1.914SerPhe: 1.914 ± 0.29
5.393SerGly: 5.393 ± 0.676
1.102SerHis: 1.102 ± 0.222
2.841SerIle: 2.841 ± 0.433
3.827SerLys: 3.827 ± 0.512
5.509SerLeu: 5.509 ± 0.61
1.45SerMet: 1.45 ± 0.282
2.378SerAsn: 2.378 ± 0.405
2.435SerPro: 2.435 ± 0.394
1.914SerGln: 1.914 ± 0.272
2.551SerArg: 2.551 ± 0.433
4.465SerSer: 4.465 ± 0.803
4.233SerThr: 4.233 ± 0.619
4.117SerVal: 4.117 ± 0.452
1.044SerTrp: 1.044 ± 0.236
2.03SerTyr: 2.03 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
6.147ThrAla: 6.147 ± 0.959
0.232ThrCys: 0.232 ± 0.128
3.885ThrAsp: 3.885 ± 0.567
4.059ThrGlu: 4.059 ± 0.485
2.841ThrPhe: 2.841 ± 0.364
5.277ThrGly: 5.277 ± 0.657
1.16ThrHis: 1.16 ± 0.269
3.595ThrIle: 3.595 ± 0.443
3.827ThrLys: 3.827 ± 0.49
5.973ThrLeu: 5.973 ± 0.625
1.392ThrMet: 1.392 ± 0.279
2.32ThrAsn: 2.32 ± 0.352
3.131ThrPro: 3.131 ± 0.489
2.03ThrGln: 2.03 ± 0.364
3.421ThrArg: 3.421 ± 0.468
3.479ThrSer: 3.479 ± 0.591
4.871ThrThr: 4.871 ± 0.674
4.871ThrVal: 4.871 ± 0.605
1.102ThrTrp: 1.102 ± 0.327
2.435ThrTyr: 2.435 ± 0.509
0.0ThrXaa: 0.0 ± 0.0
Val
5.915ValAla: 5.915 ± 0.678
0.29ValCys: 0.29 ± 0.146
4.523ValAsp: 4.523 ± 0.505
5.103ValGlu: 5.103 ± 0.582
2.493ValPhe: 2.493 ± 0.336
5.045ValGly: 5.045 ± 0.621
1.16ValHis: 1.16 ± 0.249
3.653ValIle: 3.653 ± 0.393
4.581ValLys: 4.581 ± 0.575
5.045ValLeu: 5.045 ± 0.581
2.146ValMet: 2.146 ± 0.295
3.305ValAsn: 3.305 ± 0.386
2.378ValPro: 2.378 ± 0.36
2.262ValGln: 2.262 ± 0.371
4.117ValArg: 4.117 ± 0.583
4.291ValSer: 4.291 ± 0.418
4.465ValThr: 4.465 ± 0.641
5.451ValVal: 5.451 ± 0.779
1.16ValTrp: 1.16 ± 0.256
3.189ValTyr: 3.189 ± 0.494
0.0ValXaa: 0.0 ± 0.0
Trp
1.102TrpAla: 1.102 ± 0.252
0.058TrpCys: 0.058 ± 0.069
1.16TrpAsp: 1.16 ± 0.263
0.87TrpGlu: 0.87 ± 0.215
0.232TrpPhe: 0.232 ± 0.117
0.696TrpGly: 0.696 ± 0.18
0.464TrpHis: 0.464 ± 0.17
0.58TrpIle: 0.58 ± 0.212
0.812TrpLys: 0.812 ± 0.273
1.16TrpLeu: 1.16 ± 0.293
0.348TrpMet: 0.348 ± 0.165
1.334TrpAsn: 1.334 ± 0.323
0.174TrpPro: 0.174 ± 0.096
0.348TrpGln: 0.348 ± 0.116
0.58TrpArg: 0.58 ± 0.205
1.45TrpSer: 1.45 ± 0.398
0.696TrpThr: 0.696 ± 0.218
1.044TrpVal: 1.044 ± 0.334
0.174TrpTrp: 0.174 ± 0.106
0.464TrpTyr: 0.464 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.015TyrAla: 3.015 ± 0.431
0.232TyrCys: 0.232 ± 0.126
2.609TyrAsp: 2.609 ± 0.524
1.972TyrGlu: 1.972 ± 0.348
1.74TyrPhe: 1.74 ± 0.443
2.088TyrGly: 2.088 ± 0.394
0.638TyrHis: 0.638 ± 0.225
1.334TyrIle: 1.334 ± 0.34
2.551TyrLys: 2.551 ± 0.408
2.609TyrLeu: 2.609 ± 0.444
0.348TyrMet: 0.348 ± 0.135
1.392TyrAsn: 1.392 ± 0.281
1.566TyrPro: 1.566 ± 0.293
1.334TyrGln: 1.334 ± 0.314
2.146TyrArg: 2.146 ± 0.38
2.378TyrSer: 2.378 ± 0.404
2.088TyrThr: 2.088 ± 0.383
3.421TyrVal: 3.421 ± 0.415
0.29TyrTrp: 0.29 ± 0.142
1.45TyrTyr: 1.45 ± 0.358
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (17246 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski