Amino acid dipepetide frequency for Flavobacterium phage vB_FspS_stinky9-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.745AlaAla: 0.745 ± 0.331
0.745AlaCys: 0.745 ± 0.228
1.987AlaAsp: 1.987 ± 0.391
3.064AlaGlu: 3.064 ± 0.657
2.153AlaPhe: 2.153 ± 0.459
1.904AlaGly: 1.904 ± 0.333
0.662AlaHis: 0.662 ± 0.22
4.14AlaIle: 4.14 ± 0.604
5.796AlaLys: 5.796 ± 0.7
4.471AlaLeu: 4.471 ± 0.608
1.408AlaMet: 1.408 ± 0.522
5.051AlaAsn: 5.051 ± 0.686
0.994AlaPro: 0.994 ± 0.261
1.739AlaGln: 1.739 ± 0.36
1.573AlaArg: 1.573 ± 0.48
3.312AlaSer: 3.312 ± 0.556
4.223AlaThr: 4.223 ± 0.53
3.064AlaVal: 3.064 ± 0.55
0.497AlaTrp: 0.497 ± 0.188
2.07AlaTyr: 2.07 ± 0.349
0.0AlaXaa: 0.0 ± 0.0
Cys
0.166CysAla: 0.166 ± 0.158
0.248CysCys: 0.248 ± 0.146
0.745CysAsp: 0.745 ± 0.256
1.159CysGlu: 1.159 ± 0.353
0.828CysPhe: 0.828 ± 0.255
0.911CysGly: 0.911 ± 0.293
0.083CysHis: 0.083 ± 0.084
0.248CysIle: 0.248 ± 0.142
1.076CysLys: 1.076 ± 0.351
1.159CysLeu: 1.159 ± 0.362
0.083CysMet: 0.083 ± 0.079
0.414CysAsn: 0.414 ± 0.251
0.745CysPro: 0.745 ± 0.273
0.248CysGln: 0.248 ± 0.134
0.331CysArg: 0.331 ± 0.155
0.994CysSer: 0.994 ± 0.307
0.662CysThr: 0.662 ± 0.196
0.745CysVal: 0.745 ± 0.213
0.248CysTrp: 0.248 ± 0.153
0.331CysTyr: 0.331 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
3.809AspAla: 3.809 ± 0.448
0.911AspCys: 0.911 ± 0.301
1.822AspAsp: 1.822 ± 0.456
4.14AspGlu: 4.14 ± 0.584
4.389AspPhe: 4.389 ± 0.589
2.567AspGly: 2.567 ± 0.478
0.662AspHis: 0.662 ± 0.223
3.56AspIle: 3.56 ± 0.54
5.382AspLys: 5.382 ± 0.891
5.051AspLeu: 5.051 ± 0.632
1.49AspMet: 1.49 ± 0.407
4.72AspAsn: 4.72 ± 0.67
0.497AspPro: 0.497 ± 0.178
0.497AspGln: 0.497 ± 0.243
1.408AspArg: 1.408 ± 0.235
3.643AspSer: 3.643 ± 0.54
3.643AspThr: 3.643 ± 0.575
2.898AspVal: 2.898 ± 0.652
0.745AspTrp: 0.745 ± 0.226
3.395AspTyr: 3.395 ± 0.436
0.0AspXaa: 0.0 ± 0.0
Glu
2.318GluAla: 2.318 ± 0.676
0.828GluCys: 0.828 ± 0.307
3.229GluAsp: 3.229 ± 0.576
3.974GluGlu: 3.974 ± 0.735
4.389GluPhe: 4.389 ± 0.656
1.987GluGly: 1.987 ± 0.35
0.994GluHis: 0.994 ± 0.315
7.369GluIle: 7.369 ± 0.797
7.369GluLys: 7.369 ± 0.826
7.369GluLeu: 7.369 ± 0.862
1.822GluMet: 1.822 ± 0.385
6.624GluAsn: 6.624 ± 0.823
1.822GluPro: 1.822 ± 0.396
3.643GluGln: 3.643 ± 0.559
2.484GluArg: 2.484 ± 0.607
3.809GluSer: 3.809 ± 0.521
4.14GluThr: 4.14 ± 0.599
4.306GluVal: 4.306 ± 0.527
0.414GluTrp: 0.414 ± 0.17
3.56GluTyr: 3.56 ± 0.491
0.0GluXaa: 0.0 ± 0.0
Phe
2.484PheAla: 2.484 ± 0.448
0.828PheCys: 0.828 ± 0.303
3.809PheAsp: 3.809 ± 0.543
4.306PheGlu: 4.306 ± 0.627
2.401PhePhe: 2.401 ± 0.348
2.981PheGly: 2.981 ± 0.466
0.497PheHis: 0.497 ± 0.18
3.229PheIle: 3.229 ± 0.586
4.057PheLys: 4.057 ± 0.563
3.478PheLeu: 3.478 ± 0.619
1.159PheMet: 1.159 ± 0.272
4.885PheAsn: 4.885 ± 0.829
0.994PhePro: 0.994 ± 0.24
1.822PheGln: 1.822 ± 0.453
1.325PheArg: 1.325 ± 0.352
4.14PheSer: 4.14 ± 0.6
4.306PheThr: 4.306 ± 0.671
3.064PheVal: 3.064 ± 0.522
0.497PheTrp: 0.497 ± 0.179
1.739PheTyr: 1.739 ± 0.367
0.0PheXaa: 0.0 ± 0.0
Gly
3.229GlyAla: 3.229 ± 0.644
0.331GlyCys: 0.331 ± 0.14
2.65GlyAsp: 2.65 ± 0.568
1.987GlyGlu: 1.987 ± 0.39
2.898GlyPhe: 2.898 ± 0.611
2.153GlyGly: 2.153 ± 0.602
0.331GlyHis: 0.331 ± 0.166
3.974GlyIle: 3.974 ± 0.477
2.981GlyLys: 2.981 ± 0.47
3.809GlyLeu: 3.809 ± 0.513
1.325GlyMet: 1.325 ± 0.334
4.554GlyAsn: 4.554 ± 0.635
0.0GlyPro: 0.0 ± 0.0
1.573GlyGln: 1.573 ± 0.343
1.656GlyArg: 1.656 ± 0.369
2.567GlySer: 2.567 ± 0.375
4.637GlyThr: 4.637 ± 0.858
2.732GlyVal: 2.732 ± 0.414
0.331GlyTrp: 0.331 ± 0.202
2.567GlyTyr: 2.567 ± 0.469
0.0GlyXaa: 0.0 ± 0.0
His
0.414HisAla: 0.414 ± 0.181
0.248HisCys: 0.248 ± 0.148
1.076HisAsp: 1.076 ± 0.286
0.497HisGlu: 0.497 ± 0.182
1.159HisPhe: 1.159 ± 0.282
0.911HisGly: 0.911 ± 0.303
0.745HisHis: 0.745 ± 0.304
1.242HisIle: 1.242 ± 0.32
0.828HisLys: 0.828 ± 0.293
1.076HisLeu: 1.076 ± 0.265
0.083HisMet: 0.083 ± 0.088
1.076HisAsn: 1.076 ± 0.327
0.414HisPro: 0.414 ± 0.205
0.497HisGln: 0.497 ± 0.212
0.497HisArg: 0.497 ± 0.205
1.325HisSer: 1.325 ± 0.376
0.58HisThr: 0.58 ± 0.165
0.828HisVal: 0.828 ± 0.269
0.0HisTrp: 0.0 ± 0.0
0.497HisTyr: 0.497 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
4.72IleAla: 4.72 ± 0.708
1.076IleCys: 1.076 ± 0.407
4.803IleAsp: 4.803 ± 0.483
7.535IleGlu: 7.535 ± 0.737
3.56IlePhe: 3.56 ± 0.563
4.14IleGly: 4.14 ± 0.616
1.076IleHis: 1.076 ± 0.397
5.134IleIle: 5.134 ± 0.779
8.363IleLys: 8.363 ± 0.948
6.873IleLeu: 6.873 ± 0.795
1.242IleMet: 1.242 ± 0.383
6.293IleAsn: 6.293 ± 0.707
2.401IlePro: 2.401 ± 0.413
2.401IleGln: 2.401 ± 0.495
1.739IleArg: 1.739 ± 0.344
5.051IleSer: 5.051 ± 0.557
4.306IleThr: 4.306 ± 0.751
4.554IleVal: 4.554 ± 0.686
0.994IleTrp: 0.994 ± 0.3
3.146IleTyr: 3.146 ± 0.466
0.0IleXaa: 0.0 ± 0.0
Lys
5.465LysAla: 5.465 ± 0.712
1.159LysCys: 1.159 ± 0.335
4.389LysAsp: 4.389 ± 0.548
9.191LysGlu: 9.191 ± 1.226
3.229LysPhe: 3.229 ± 0.447
3.974LysGly: 3.974 ± 0.539
1.987LysHis: 1.987 ± 0.461
7.783LysIle: 7.783 ± 0.751
7.287LysLys: 7.287 ± 1.005
7.535LysLeu: 7.535 ± 0.8
3.395LysMet: 3.395 ± 0.489
6.21LysAsn: 6.21 ± 0.669
2.401LysPro: 2.401 ± 0.466
4.471LysGln: 4.471 ± 0.827
3.56LysArg: 3.56 ± 0.676
5.299LysSer: 5.299 ± 0.585
5.796LysThr: 5.796 ± 0.665
5.051LysVal: 5.051 ± 0.707
1.408LysTrp: 1.408 ± 0.403
4.223LysTyr: 4.223 ± 0.609
0.0LysXaa: 0.0 ± 0.0
Leu
4.223LeuAla: 4.223 ± 0.709
0.58LeuCys: 0.58 ± 0.287
5.299LeuAsp: 5.299 ± 0.623
6.459LeuGlu: 6.459 ± 0.946
4.223LeuPhe: 4.223 ± 0.831
3.726LeuGly: 3.726 ± 0.423
0.911LeuHis: 0.911 ± 0.284
6.624LeuIle: 6.624 ± 0.825
8.943LeuLys: 8.943 ± 0.816
6.624LeuLeu: 6.624 ± 0.92
1.739LeuMet: 1.739 ± 0.46
7.038LeuAsn: 7.038 ± 0.876
3.229LeuPro: 3.229 ± 0.499
4.389LeuGln: 4.389 ± 0.609
3.395LeuArg: 3.395 ± 0.608
5.796LeuSer: 5.796 ± 0.689
5.962LeuThr: 5.962 ± 0.582
4.803LeuVal: 4.803 ± 0.68
0.662LeuTrp: 0.662 ± 0.266
4.057LeuTyr: 4.057 ± 0.652
0.0LeuXaa: 0.0 ± 0.0
Met
1.656MetAla: 1.656 ± 0.362
0.083MetCys: 0.083 ± 0.081
0.745MetAsp: 0.745 ± 0.289
1.49MetGlu: 1.49 ± 0.288
1.325MetPhe: 1.325 ± 0.292
0.58MetGly: 0.58 ± 0.223
0.083MetHis: 0.083 ± 0.093
1.325MetIle: 1.325 ± 0.377
2.65MetLys: 2.65 ± 0.628
1.822MetLeu: 1.822 ± 0.412
0.414MetMet: 0.414 ± 0.159
1.49MetAsn: 1.49 ± 0.37
0.994MetPro: 0.994 ± 0.284
0.994MetGln: 0.994 ± 0.238
0.994MetArg: 0.994 ± 0.243
2.07MetSer: 2.07 ± 0.416
1.076MetThr: 1.076 ± 0.296
0.911MetVal: 0.911 ± 0.209
0.331MetTrp: 0.331 ± 0.152
0.58MetTyr: 0.58 ± 0.232
0.0MetXaa: 0.0 ± 0.0
Asn
4.554AsnAla: 4.554 ± 0.611
0.58AsnCys: 0.58 ± 0.209
4.968AsnAsp: 4.968 ± 0.726
5.879AsnGlu: 5.879 ± 0.762
3.974AsnPhe: 3.974 ± 0.734
4.057AsnGly: 4.057 ± 0.564
1.159AsnHis: 1.159 ± 0.326
5.299AsnIle: 5.299 ± 0.5
9.357AsnLys: 9.357 ± 1.066
7.204AsnLeu: 7.204 ± 0.887
1.159AsnMet: 1.159 ± 0.434
4.389AsnAsn: 4.389 ± 1.053
2.236AsnPro: 2.236 ± 0.425
3.146AsnGln: 3.146 ± 0.508
1.987AsnArg: 1.987 ± 0.454
5.217AsnSer: 5.217 ± 0.683
4.306AsnThr: 4.306 ± 0.504
5.713AsnVal: 5.713 ± 0.675
0.745AsnTrp: 0.745 ± 0.267
4.72AsnTyr: 4.72 ± 0.622
0.0AsnXaa: 0.0 ± 0.0
Pro
1.408ProAla: 1.408 ± 0.308
0.497ProCys: 0.497 ± 0.191
1.076ProAsp: 1.076 ± 0.303
1.739ProGlu: 1.739 ± 0.458
1.325ProPhe: 1.325 ± 0.291
0.0ProGly: 0.0 ± 0.0
0.083ProHis: 0.083 ± 0.072
1.159ProIle: 1.159 ± 0.27
1.573ProLys: 1.573 ± 0.354
2.732ProLeu: 2.732 ± 0.497
0.828ProMet: 0.828 ± 0.289
2.318ProAsn: 2.318 ± 0.433
0.745ProPro: 0.745 ± 0.354
1.325ProGln: 1.325 ± 0.39
0.331ProArg: 0.331 ± 0.155
2.401ProSer: 2.401 ± 0.479
2.153ProThr: 2.153 ± 0.412
1.408ProVal: 1.408 ± 0.299
0.0ProTrp: 0.0 ± 0.0
1.325ProTyr: 1.325 ± 0.385
0.0ProXaa: 0.0 ± 0.0
Gln
2.07GlnAla: 2.07 ± 0.691
0.331GlnCys: 0.331 ± 0.191
1.325GlnAsp: 1.325 ± 0.342
2.153GlnGlu: 2.153 ± 0.454
1.242GlnPhe: 1.242 ± 0.277
2.07GlnGly: 2.07 ± 0.411
0.662GlnHis: 0.662 ± 0.214
4.471GlnIle: 4.471 ± 0.573
3.726GlnLys: 3.726 ± 0.695
3.892GlnLeu: 3.892 ± 0.49
0.911GlnMet: 0.911 ± 0.34
2.484GlnAsn: 2.484 ± 0.469
0.994GlnPro: 0.994 ± 0.318
1.822GlnGln: 1.822 ± 0.775
1.987GlnArg: 1.987 ± 0.498
2.318GlnSer: 2.318 ± 0.37
2.484GlnThr: 2.484 ± 0.478
2.318GlnVal: 2.318 ± 0.38
0.331GlnTrp: 0.331 ± 0.164
1.656GlnTyr: 1.656 ± 0.431
0.0GlnXaa: 0.0 ± 0.0
Arg
1.242ArgAla: 1.242 ± 0.322
0.58ArgCys: 0.58 ± 0.198
1.242ArgAsp: 1.242 ± 0.31
1.987ArgGlu: 1.987 ± 0.474
1.159ArgPhe: 1.159 ± 0.308
0.994ArgGly: 0.994 ± 0.293
0.414ArgHis: 0.414 ± 0.202
2.981ArgIle: 2.981 ± 0.572
2.815ArgLys: 2.815 ± 0.428
3.229ArgLeu: 3.229 ± 0.409
0.662ArgMet: 0.662 ± 0.191
2.898ArgAsn: 2.898 ± 0.458
0.166ArgPro: 0.166 ± 0.103
0.994ArgGln: 0.994 ± 0.32
0.911ArgArg: 0.911 ± 0.258
1.904ArgSer: 1.904 ± 0.358
2.153ArgThr: 2.153 ± 0.428
2.153ArgVal: 2.153 ± 0.34
0.166ArgTrp: 0.166 ± 0.13
1.987ArgTyr: 1.987 ± 0.43
0.0ArgXaa: 0.0 ± 0.0
Ser
1.987SerAla: 1.987 ± 0.333
0.414SerCys: 0.414 ± 0.181
4.223SerAsp: 4.223 ± 0.706
5.217SerGlu: 5.217 ± 0.758
4.306SerPhe: 4.306 ± 0.697
4.223SerGly: 4.223 ± 0.599
0.911SerHis: 0.911 ± 0.221
5.548SerIle: 5.548 ± 0.691
5.962SerLys: 5.962 ± 0.811
6.459SerLeu: 6.459 ± 0.92
1.159SerMet: 1.159 ± 0.272
5.631SerAsn: 5.631 ± 0.799
1.325SerPro: 1.325 ± 0.317
2.732SerGln: 2.732 ± 0.493
1.822SerArg: 1.822 ± 0.36
3.809SerSer: 3.809 ± 0.632
2.732SerThr: 2.732 ± 0.581
4.968SerVal: 4.968 ± 0.563
0.662SerTrp: 0.662 ± 0.261
1.325SerTyr: 1.325 ± 0.294
0.0SerXaa: 0.0 ± 0.0
Thr
4.057ThrAla: 4.057 ± 0.622
0.497ThrCys: 0.497 ± 0.185
4.72ThrAsp: 4.72 ± 0.615
4.554ThrGlu: 4.554 ± 0.82
3.809ThrPhe: 3.809 ± 0.626
3.229ThrGly: 3.229 ± 0.622
0.911ThrHis: 0.911 ± 0.266
6.376ThrIle: 6.376 ± 0.825
4.471ThrLys: 4.471 ± 0.508
5.962ThrLeu: 5.962 ± 0.559
1.076ThrMet: 1.076 ± 0.374
4.968ThrAsn: 4.968 ± 0.744
1.987ThrPro: 1.987 ± 0.421
3.064ThrGln: 3.064 ± 0.501
1.408ThrArg: 1.408 ± 0.285
3.809ThrSer: 3.809 ± 0.661
5.051ThrThr: 5.051 ± 0.901
1.408ThrVal: 1.408 ± 0.345
0.662ThrTrp: 0.662 ± 0.247
2.318ThrTyr: 2.318 ± 0.448
0.0ThrXaa: 0.0 ± 0.0
Val
2.981ValAla: 2.981 ± 0.536
0.745ValCys: 0.745 ± 0.277
3.229ValAsp: 3.229 ± 0.496
3.229ValGlu: 3.229 ± 0.6
2.65ValPhe: 2.65 ± 0.486
3.395ValGly: 3.395 ± 0.416
0.662ValHis: 0.662 ± 0.191
4.14ValIle: 4.14 ± 0.483
5.299ValLys: 5.299 ± 0.582
4.885ValLeu: 4.885 ± 0.646
1.242ValMet: 1.242 ± 0.282
5.382ValAsn: 5.382 ± 0.784
1.242ValPro: 1.242 ± 0.323
2.401ValGln: 2.401 ± 0.454
1.49ValArg: 1.49 ± 0.424
4.389ValSer: 4.389 ± 0.579
2.65ValThr: 2.65 ± 0.537
2.732ValVal: 2.732 ± 0.613
0.662ValTrp: 0.662 ± 0.2
2.484ValTyr: 2.484 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
0.414TrpAla: 0.414 ± 0.167
0.166TrpCys: 0.166 ± 0.123
0.828TrpAsp: 0.828 ± 0.315
0.58TrpGlu: 0.58 ± 0.194
0.497TrpPhe: 0.497 ± 0.199
0.331TrpGly: 0.331 ± 0.158
0.414TrpHis: 0.414 ± 0.207
0.994TrpIle: 0.994 ± 0.319
0.994TrpLys: 0.994 ± 0.402
1.159TrpLeu: 1.159 ± 0.245
0.0TrpMet: 0.0 ± 0.0
0.828TrpAsn: 0.828 ± 0.259
0.0TrpPro: 0.0 ± 0.0
0.248TrpGln: 0.248 ± 0.139
0.414TrpArg: 0.414 ± 0.177
0.745TrpSer: 0.745 ± 0.296
0.662TrpThr: 0.662 ± 0.23
0.166TrpVal: 0.166 ± 0.135
0.0TrpTrp: 0.0 ± 0.0
0.58TrpTyr: 0.58 ± 0.238
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.656TyrAla: 1.656 ± 0.28
0.58TyrCys: 0.58 ± 0.224
3.064TyrAsp: 3.064 ± 0.516
3.395TyrGlu: 3.395 ± 0.47
2.484TyrPhe: 2.484 ± 0.503
2.153TyrGly: 2.153 ± 0.324
0.745TyrHis: 0.745 ± 0.289
3.809TyrIle: 3.809 ± 0.563
4.885TyrLys: 4.885 ± 0.668
3.892TyrLeu: 3.892 ± 0.482
0.331TyrMet: 0.331 ± 0.148
3.395TyrAsn: 3.395 ± 0.541
1.242TyrPro: 1.242 ± 0.374
1.242TyrGln: 1.242 ± 0.297
1.325TyrArg: 1.325 ± 0.416
2.815TyrSer: 2.815 ± 0.507
2.815TyrThr: 2.815 ± 0.573
2.07TyrVal: 2.07 ± 0.45
0.662TyrTrp: 0.662 ± 0.261
2.318TyrTyr: 2.318 ± 0.438
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (12078 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski