Amino acid dipepetide frequency for Bifidobacterium phage BigBern1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.301AlaAla: 11.301 ± 1.228
1.005AlaCys: 1.005 ± 0.349
7.283AlaAsp: 7.283 ± 0.939
4.939AlaGlu: 4.939 ± 0.896
2.428AlaPhe: 2.428 ± 0.636
9.459AlaGly: 9.459 ± 1.213
2.093AlaHis: 2.093 ± 0.484
4.855AlaIle: 4.855 ± 0.781
5.19AlaLys: 5.19 ± 0.809
9.041AlaLeu: 9.041 ± 0.909
4.102AlaMet: 4.102 ± 0.747
1.925AlaAsn: 1.925 ± 0.508
5.19AlaPro: 5.19 ± 0.754
5.106AlaGln: 5.106 ± 0.74
5.609AlaArg: 5.609 ± 0.74
6.948AlaSer: 6.948 ± 0.84
4.688AlaThr: 4.688 ± 0.647
6.948AlaVal: 6.948 ± 0.908
2.093AlaTrp: 2.093 ± 0.482
3.097AlaTyr: 3.097 ± 0.451
0.0AlaXaa: 0.0 ± 0.0
Cys
0.67CysAla: 0.67 ± 0.24
0.084CysCys: 0.084 ± 0.092
1.088CysAsp: 1.088 ± 0.321
0.502CysGlu: 0.502 ± 0.202
0.251CysPhe: 0.251 ± 0.158
1.256CysGly: 1.256 ± 0.412
0.167CysHis: 0.167 ± 0.118
0.502CysIle: 0.502 ± 0.224
0.921CysLys: 0.921 ± 0.317
1.423CysLeu: 1.423 ± 0.369
0.084CysMet: 0.084 ± 0.088
0.167CysAsn: 0.167 ± 0.118
1.005CysPro: 1.005 ± 0.303
0.753CysGln: 0.753 ± 0.23
0.837CysArg: 0.837 ± 0.287
0.502CysSer: 0.502 ± 0.181
0.67CysThr: 0.67 ± 0.279
0.419CysVal: 0.419 ± 0.204
0.167CysTrp: 0.167 ± 0.123
0.502CysTyr: 0.502 ± 0.19
0.0CysXaa: 0.0 ± 0.0
Asp
6.111AspAla: 6.111 ± 0.607
0.921AspCys: 0.921 ± 0.26
3.683AspAsp: 3.683 ± 0.462
4.771AspGlu: 4.771 ± 0.635
1.423AspPhe: 1.423 ± 0.371
8.371AspGly: 8.371 ± 1.052
1.59AspHis: 1.59 ± 0.443
2.762AspIle: 2.762 ± 0.492
2.344AspLys: 2.344 ± 0.441
5.023AspLeu: 5.023 ± 0.743
2.344AspMet: 2.344 ± 0.384
1.674AspAsn: 1.674 ± 0.364
4.186AspPro: 4.186 ± 0.647
3.265AspGln: 3.265 ± 0.502
5.106AspArg: 5.106 ± 0.735
3.851AspSer: 3.851 ± 0.611
4.102AspThr: 4.102 ± 0.63
3.6AspVal: 3.6 ± 0.492
1.842AspTrp: 1.842 ± 0.421
2.009AspTyr: 2.009 ± 0.471
0.0AspXaa: 0.0 ± 0.0
Glu
5.943GluAla: 5.943 ± 0.882
0.502GluCys: 0.502 ± 0.228
3.683GluAsp: 3.683 ± 0.75
3.851GluGlu: 3.851 ± 0.749
1.256GluPhe: 1.256 ± 0.346
2.679GluGly: 2.679 ± 0.474
1.423GluHis: 1.423 ± 0.314
2.511GluIle: 2.511 ± 0.448
2.511GluLys: 2.511 ± 0.465
4.018GluLeu: 4.018 ± 0.694
1.423GluMet: 1.423 ± 0.396
1.339GluAsn: 1.339 ± 0.328
2.511GluPro: 2.511 ± 0.608
3.516GluGln: 3.516 ± 0.576
3.934GluArg: 3.934 ± 0.604
2.176GluSer: 2.176 ± 0.573
3.432GluThr: 3.432 ± 0.559
2.595GluVal: 2.595 ± 0.489
1.088GluTrp: 1.088 ± 0.282
1.674GluTyr: 1.674 ± 0.341
0.0GluXaa: 0.0 ± 0.0
Phe
2.679PheAla: 2.679 ± 0.523
0.167PheCys: 0.167 ± 0.109
1.507PheAsp: 1.507 ± 0.274
1.339PheGlu: 1.339 ± 0.393
0.335PhePhe: 0.335 ± 0.176
2.428PheGly: 2.428 ± 0.629
0.251PheHis: 0.251 ± 0.163
1.172PheIle: 1.172 ± 0.296
0.67PheLys: 0.67 ± 0.212
1.172PheLeu: 1.172 ± 0.319
0.67PheMet: 0.67 ± 0.258
0.753PheAsn: 0.753 ± 0.268
1.256PhePro: 1.256 ± 0.346
0.586PheGln: 0.586 ± 0.215
1.005PheArg: 1.005 ± 0.306
2.26PheSer: 2.26 ± 0.767
1.507PheThr: 1.507 ± 0.367
1.507PheVal: 1.507 ± 0.267
0.502PheTrp: 0.502 ± 0.265
0.586PheTyr: 0.586 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
7.366GlyAla: 7.366 ± 1.141
1.256GlyCys: 1.256 ± 0.347
5.86GlyAsp: 5.86 ± 0.938
3.767GlyGlu: 3.767 ± 0.531
2.595GlyPhe: 2.595 ± 0.495
6.781GlyGly: 6.781 ± 0.842
1.423GlyHis: 1.423 ± 0.367
4.855GlyIle: 4.855 ± 0.806
4.437GlyLys: 4.437 ± 0.7
7.534GlyLeu: 7.534 ± 0.776
2.762GlyMet: 2.762 ± 0.463
3.097GlyAsn: 3.097 ± 0.713
2.762GlyPro: 2.762 ± 0.429
3.851GlyGln: 3.851 ± 0.565
6.613GlyArg: 6.613 ± 0.815
5.776GlySer: 5.776 ± 0.856
5.19GlyThr: 5.19 ± 0.752
4.939GlyVal: 4.939 ± 0.651
1.674GlyTrp: 1.674 ± 0.361
2.511GlyTyr: 2.511 ± 0.488
0.0GlyXaa: 0.0 ± 0.0
His
2.595HisAla: 2.595 ± 0.535
0.251HisCys: 0.251 ± 0.142
1.758HisAsp: 1.758 ± 0.393
0.921HisGlu: 0.921 ± 0.284
0.67HisPhe: 0.67 ± 0.205
2.344HisGly: 2.344 ± 0.423
0.67HisHis: 0.67 ± 0.348
1.423HisIle: 1.423 ± 0.353
0.586HisLys: 0.586 ± 0.314
0.837HisLeu: 0.837 ± 0.244
0.419HisMet: 0.419 ± 0.184
0.335HisAsn: 0.335 ± 0.126
1.423HisPro: 1.423 ± 0.353
0.753HisGln: 0.753 ± 0.24
1.423HisArg: 1.423 ± 0.369
0.837HisSer: 0.837 ± 0.27
0.837HisThr: 0.837 ± 0.285
1.59HisVal: 1.59 ± 0.326
0.251HisTrp: 0.251 ± 0.131
0.251HisTyr: 0.251 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
5.357IleAla: 5.357 ± 0.68
0.67IleCys: 0.67 ± 0.244
4.102IleAsp: 4.102 ± 0.618
1.339IleGlu: 1.339 ± 0.325
1.088IlePhe: 1.088 ± 0.271
4.186IleGly: 4.186 ± 0.672
0.921IleHis: 0.921 ± 0.263
1.842IleIle: 1.842 ± 0.46
1.59IleLys: 1.59 ± 0.351
2.428IleLeu: 2.428 ± 0.397
1.005IleMet: 1.005 ± 0.289
1.758IleAsn: 1.758 ± 0.48
3.683IlePro: 3.683 ± 0.508
2.762IleGln: 2.762 ± 0.416
4.102IleArg: 4.102 ± 0.476
3.6IleSer: 3.6 ± 0.75
3.683IleThr: 3.683 ± 0.673
3.097IleVal: 3.097 ± 0.622
1.005IleTrp: 1.005 ± 0.335
1.256IleTyr: 1.256 ± 0.424
0.0IleXaa: 0.0 ± 0.0
Lys
4.855LysAla: 4.855 ± 0.823
0.419LysCys: 0.419 ± 0.19
3.265LysAsp: 3.265 ± 0.628
2.428LysGlu: 2.428 ± 0.562
0.502LysPhe: 0.502 ± 0.243
3.265LysGly: 3.265 ± 0.472
0.921LysHis: 0.921 ± 0.276
1.758LysIle: 1.758 ± 0.422
2.176LysLys: 2.176 ± 0.519
2.428LysLeu: 2.428 ± 0.478
0.837LysMet: 0.837 ± 0.212
1.088LysAsn: 1.088 ± 0.388
2.176LysPro: 2.176 ± 0.461
2.595LysGln: 2.595 ± 0.456
3.181LysArg: 3.181 ± 0.526
2.344LysSer: 2.344 ± 0.468
3.767LysThr: 3.767 ± 0.647
2.93LysVal: 2.93 ± 0.475
0.67LysTrp: 0.67 ± 0.24
1.758LysTyr: 1.758 ± 0.4
0.0LysXaa: 0.0 ± 0.0
Leu
9.376LeuAla: 9.376 ± 0.892
1.172LeuCys: 1.172 ± 0.415
5.19LeuAsp: 5.19 ± 0.776
3.516LeuGlu: 3.516 ± 0.672
1.925LeuPhe: 1.925 ± 0.506
6.278LeuGly: 6.278 ± 0.914
2.26LeuHis: 2.26 ± 0.464
3.934LeuIle: 3.934 ± 0.477
3.851LeuLys: 3.851 ± 0.589
6.111LeuLeu: 6.111 ± 0.975
1.256LeuMet: 1.256 ± 0.342
2.344LeuAsn: 2.344 ± 0.468
4.437LeuPro: 4.437 ± 0.936
3.683LeuGln: 3.683 ± 0.545
5.106LeuArg: 5.106 ± 0.757
6.781LeuSer: 6.781 ± 1.107
5.274LeuThr: 5.274 ± 0.61
4.437LeuVal: 4.437 ± 0.666
1.088LeuTrp: 1.088 ± 0.29
1.842LeuTyr: 1.842 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
3.097MetAla: 3.097 ± 0.412
0.502MetCys: 0.502 ± 0.201
1.256MetAsp: 1.256 ± 0.308
1.088MetGlu: 1.088 ± 0.261
0.167MetPhe: 0.167 ± 0.122
2.093MetGly: 2.093 ± 0.428
0.502MetHis: 0.502 ± 0.193
1.088MetIle: 1.088 ± 0.259
1.088MetLys: 1.088 ± 0.334
2.762MetLeu: 2.762 ± 0.429
0.837MetMet: 0.837 ± 0.298
0.921MetAsn: 0.921 ± 0.252
2.176MetPro: 2.176 ± 0.543
1.005MetGln: 1.005 ± 0.342
1.507MetArg: 1.507 ± 0.336
2.846MetSer: 2.846 ± 0.541
2.344MetThr: 2.344 ± 0.422
1.842MetVal: 1.842 ± 0.466
0.419MetTrp: 0.419 ± 0.163
0.502MetTyr: 0.502 ± 0.225
0.0MetXaa: 0.0 ± 0.0
Asn
4.353AsnAla: 4.353 ± 0.884
0.084AsnCys: 0.084 ± 0.082
1.423AsnAsp: 1.423 ± 0.356
1.172AsnGlu: 1.172 ± 0.297
0.419AsnPhe: 0.419 ± 0.199
3.265AsnGly: 3.265 ± 0.722
0.753AsnHis: 0.753 ± 0.281
1.088AsnIle: 1.088 ± 0.293
1.256AsnLys: 1.256 ± 0.432
2.679AsnLeu: 2.679 ± 0.438
0.67AsnMet: 0.67 ± 0.219
1.172AsnAsn: 1.172 ± 0.277
1.59AsnPro: 1.59 ± 0.393
1.088AsnGln: 1.088 ± 0.264
1.674AsnArg: 1.674 ± 0.44
1.842AsnSer: 1.842 ± 0.404
0.837AsnThr: 0.837 ± 0.328
1.674AsnVal: 1.674 ± 0.496
0.753AsnTrp: 0.753 ± 0.219
0.502AsnTyr: 0.502 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
6.362ProAla: 6.362 ± 0.724
0.335ProCys: 0.335 ± 0.172
5.357ProAsp: 5.357 ± 0.75
4.855ProGlu: 4.855 ± 0.668
0.837ProPhe: 0.837 ± 0.334
4.018ProGly: 4.018 ± 0.605
0.921ProHis: 0.921 ± 0.305
2.176ProIle: 2.176 ± 0.42
1.925ProLys: 1.925 ± 0.514
4.186ProLeu: 4.186 ± 0.695
1.005ProMet: 1.005 ± 0.258
1.339ProAsn: 1.339 ± 0.299
2.428ProPro: 2.428 ± 0.399
2.344ProGln: 2.344 ± 0.426
2.93ProArg: 2.93 ± 0.614
3.181ProSer: 3.181 ± 0.572
2.679ProThr: 2.679 ± 0.388
3.851ProVal: 3.851 ± 0.548
0.921ProTrp: 0.921 ± 0.217
1.925ProTyr: 1.925 ± 0.546
0.0ProXaa: 0.0 ± 0.0
Gln
6.529GlnAla: 6.529 ± 0.883
0.167GlnCys: 0.167 ± 0.134
2.93GlnAsp: 2.93 ± 0.485
2.679GlnGlu: 2.679 ± 0.52
0.753GlnPhe: 0.753 ± 0.186
3.934GlnGly: 3.934 ± 0.614
0.586GlnHis: 0.586 ± 0.204
2.595GlnIle: 2.595 ± 0.497
1.507GlnLys: 1.507 ± 0.337
4.52GlnLeu: 4.52 ± 0.804
1.758GlnMet: 1.758 ± 0.375
1.256GlnAsn: 1.256 ± 0.323
1.925GlnPro: 1.925 ± 0.329
3.348GlnGln: 3.348 ± 0.663
3.516GlnArg: 3.516 ± 0.664
2.428GlnSer: 2.428 ± 0.4
2.511GlnThr: 2.511 ± 0.53
3.181GlnVal: 3.181 ± 0.51
1.423GlnTrp: 1.423 ± 0.331
1.925GlnTyr: 1.925 ± 0.478
0.0GlnXaa: 0.0 ± 0.0
Arg
6.195ArgAla: 6.195 ± 0.654
1.088ArgCys: 1.088 ± 0.358
4.437ArgAsp: 4.437 ± 0.718
3.014ArgGlu: 3.014 ± 0.641
1.842ArgPhe: 1.842 ± 0.421
3.767ArgGly: 3.767 ± 0.541
1.423ArgHis: 1.423 ± 0.405
4.353ArgIle: 4.353 ± 0.614
3.516ArgLys: 3.516 ± 0.658
6.278ArgLeu: 6.278 ± 0.854
2.176ArgMet: 2.176 ± 0.516
2.176ArgAsn: 2.176 ± 0.462
3.014ArgPro: 3.014 ± 0.515
3.432ArgGln: 3.432 ± 0.611
5.943ArgArg: 5.943 ± 1.08
3.348ArgSer: 3.348 ± 0.62
3.934ArgThr: 3.934 ± 0.629
3.516ArgVal: 3.516 ± 0.497
1.842ArgTrp: 1.842 ± 0.382
2.428ArgTyr: 2.428 ± 0.557
0.0ArgXaa: 0.0 ± 0.0
Ser
5.86SerAla: 5.86 ± 0.864
0.586SerCys: 0.586 ± 0.202
4.269SerAsp: 4.269 ± 0.658
3.683SerGlu: 3.683 ± 0.649
1.842SerPhe: 1.842 ± 0.481
6.613SerGly: 6.613 ± 0.825
1.088SerHis: 1.088 ± 0.313
3.014SerIle: 3.014 ± 0.461
2.344SerLys: 2.344 ± 0.611
5.274SerLeu: 5.274 ± 0.693
2.093SerMet: 2.093 ± 0.478
1.842SerAsn: 1.842 ± 0.363
3.348SerPro: 3.348 ± 0.418
3.265SerGln: 3.265 ± 0.495
4.437SerArg: 4.437 ± 0.813
3.683SerSer: 3.683 ± 0.632
4.102SerThr: 4.102 ± 0.65
4.018SerVal: 4.018 ± 0.9
1.172SerTrp: 1.172 ± 0.288
1.842SerTyr: 1.842 ± 0.446
0.0SerXaa: 0.0 ± 0.0
Thr
5.441ThrAla: 5.441 ± 0.663
1.088ThrCys: 1.088 ± 0.328
3.767ThrAsp: 3.767 ± 0.632
2.093ThrGlu: 2.093 ± 0.441
1.423ThrPhe: 1.423 ± 0.321
5.525ThrGly: 5.525 ± 0.884
0.753ThrHis: 0.753 ± 0.274
3.432ThrIle: 3.432 ± 0.654
2.679ThrLys: 2.679 ± 0.506
5.274ThrLeu: 5.274 ± 0.619
1.339ThrMet: 1.339 ± 0.29
1.256ThrAsn: 1.256 ± 0.363
4.018ThrPro: 4.018 ± 0.584
1.925ThrGln: 1.925 ± 0.357
3.6ThrArg: 3.6 ± 0.655
3.683ThrSer: 3.683 ± 0.633
3.683ThrThr: 3.683 ± 0.578
5.19ThrVal: 5.19 ± 0.688
1.256ThrTrp: 1.256 ± 0.327
2.344ThrTyr: 2.344 ± 0.6
0.0ThrXaa: 0.0 ± 0.0
Val
5.274ValAla: 5.274 ± 0.768
0.837ValCys: 0.837 ± 0.237
5.274ValAsp: 5.274 ± 0.683
3.516ValGlu: 3.516 ± 0.594
1.507ValPhe: 1.507 ± 0.317
4.353ValGly: 4.353 ± 0.913
1.005ValHis: 1.005 ± 0.256
3.851ValIle: 3.851 ± 0.469
2.679ValLys: 2.679 ± 0.557
4.102ValLeu: 4.102 ± 0.551
1.758ValMet: 1.758 ± 0.362
2.26ValAsn: 2.26 ± 0.495
3.683ValPro: 3.683 ± 0.514
3.767ValGln: 3.767 ± 0.589
3.265ValArg: 3.265 ± 0.489
5.023ValSer: 5.023 ± 0.906
3.767ValThr: 3.767 ± 0.612
4.102ValVal: 4.102 ± 0.637
1.005ValTrp: 1.005 ± 0.254
1.088ValTyr: 1.088 ± 0.3
0.0ValXaa: 0.0 ± 0.0
Trp
1.423TrpAla: 1.423 ± 0.348
0.419TrpCys: 0.419 ± 0.197
1.172TrpAsp: 1.172 ± 0.31
1.088TrpGlu: 1.088 ± 0.33
0.419TrpPhe: 0.419 ± 0.208
1.005TrpGly: 1.005 ± 0.353
0.586TrpHis: 0.586 ± 0.223
1.172TrpIle: 1.172 ± 0.364
0.586TrpLys: 0.586 ± 0.229
2.679TrpLeu: 2.679 ± 0.512
0.586TrpMet: 0.586 ± 0.191
0.921TrpAsn: 0.921 ± 0.217
1.088TrpPro: 1.088 ± 0.312
0.921TrpGln: 0.921 ± 0.266
1.59TrpArg: 1.59 ± 0.293
1.507TrpSer: 1.507 ± 0.386
1.088TrpThr: 1.088 ± 0.31
0.921TrpVal: 0.921 ± 0.261
0.67TrpTrp: 0.67 ± 0.274
0.67TrpTyr: 0.67 ± 0.185
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.511TyrAla: 2.511 ± 0.499
0.419TyrCys: 0.419 ± 0.191
1.674TyrAsp: 1.674 ± 0.325
1.507TyrGlu: 1.507 ± 0.385
0.67TyrPhe: 0.67 ± 0.294
3.432TyrGly: 3.432 ± 0.658
0.837TyrHis: 0.837 ± 0.233
1.172TyrIle: 1.172 ± 0.363
1.507TyrLys: 1.507 ± 0.375
2.093TyrLeu: 2.093 ± 0.432
0.837TyrMet: 0.837 ± 0.291
0.67TyrAsn: 0.67 ± 0.271
1.674TyrPro: 1.674 ± 0.359
1.507TyrGln: 1.507 ± 0.439
2.344TyrArg: 2.344 ± 0.583
1.758TyrSer: 1.758 ± 0.321
1.674TyrThr: 1.674 ± 0.45
1.758TyrVal: 1.758 ± 0.374
0.67TyrTrp: 0.67 ± 0.225
0.837TyrTyr: 0.837 ± 0.226
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (11947 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski