Escherichia phage wV8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Felixounavirus; Escherichia virus wV8

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 140 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5H7F0|C5H7F0_9CAUD Uncharacterized protein 22 OS=Escherichia phage wV8 OX=576791 GN=22 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.8CFHH5 pKa = 6.61GTTQEE10 pKa = 3.93NFINLINNGDD20 pKa = 3.85KK21 pKa = 10.86PSGVWNCSDD30 pKa = 3.36MDD32 pKa = 4.21GNFYY36 pKa = 10.87VYY38 pKa = 10.27PEE40 pKa = 4.0NKK42 pKa = 10.21LYY44 pKa = 10.96GDD46 pKa = 4.46DD47 pKa = 3.9EE48 pKa = 4.67EE49 pKa = 5.76EE50 pKa = 4.09IASEE54 pKa = 5.25GIQQALGNATITAAFQMKK72 pKa = 7.87TQNIVILEE80 pKa = 4.02LDD82 pKa = 3.41IPEE85 pKa = 5.73DD86 pKa = 4.11EE87 pKa = 5.46LNDD90 pKa = 5.32DD91 pKa = 4.11YY92 pKa = 12.19SCDD95 pKa = 3.43NMSNIASFTEE105 pKa = 4.15YY106 pKa = 10.95FDD108 pKa = 4.13VNWIKK113 pKa = 10.63KK114 pKa = 9.94VYY116 pKa = 6.99TTEE119 pKa = 3.88FNAMYY124 pKa = 10.53SPFCLPSLDD133 pKa = 4.18NPNLNYY139 pKa = 10.29LDD141 pKa = 3.79EE142 pKa = 4.45TLEE145 pKa = 4.18LLAKK149 pKa = 10.31SIQQSDD155 pKa = 4.52SIQVFCDD162 pKa = 2.88IMDD165 pKa = 4.26TLTEE169 pKa = 4.53NIVEE173 pKa = 4.21SDD175 pKa = 3.5LKK177 pKa = 11.51VFFF180 pKa = 5.25

Molecular weight: 20.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5H7H7|C5H7H7_9CAUD Uncharacterized protein xxx OS=Escherichia phage wV8 OX=576791 GN=xxx PE=4 SV=1
MM1 pKa = 7.63CLFGFKK7 pKa = 10.5SRR9 pKa = 11.84IYY11 pKa = 10.42PFTLCGRR18 pKa = 11.84ILTSKK23 pKa = 10.7LNNLSTKK30 pKa = 10.48VITRR34 pKa = 11.84PLFIILSATITPTRR48 pKa = 11.84HH49 pKa = 5.82LPSEE53 pKa = 4.58GIEE56 pKa = 3.97PSPFLYY62 pKa = 10.16RR63 pKa = 11.84RR64 pKa = 11.84NRR66 pKa = 11.84LFKK69 pKa = 9.94FCCKK73 pKa = 9.93RR74 pKa = 11.84SSVNLLSQTKK84 pKa = 8.94FF85 pKa = 3.13

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

140

0

140

26496

38

906

189.3

21.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.824 ± 0.289

1.453 ± 0.099

6.141 ± 0.187

6.744 ± 0.24

4.253 ± 0.155

6.337 ± 0.209

1.966 ± 0.141

6.099 ± 0.17

7.975 ± 0.278

7.911 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.161

5.261 ± 0.181

3.031 ± 0.123

3.431 ± 0.171

4.201 ± 0.133

6.624 ± 0.189

6.337 ± 0.287

7.012 ± 0.176

1.162 ± 0.085

4.352 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski