Cylicostephanus goldi (Nematode worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Strongylida; Strongyloidea; Strongylidae; Cyathostominae; Cylicostephanus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13786 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P6RQP3|A0A3P6RQP3_CYLGO Uncharacterized protein (Fragment) OS=Cylicostephanus goldi OX=71465 GN=CGOC_LOCUS3861 PE=4 SV=1
MM1 pKa = 7.55CKK3 pKa = 9.08STSRR7 pKa = 11.84VNTAVLHH14 pKa = 5.5YY15 pKa = 8.78MDD17 pKa = 4.57SLRR20 pKa = 11.84SQRR23 pKa = 11.84RR24 pKa = 11.84AIAPHH29 pKa = 7.22DD30 pKa = 3.7ISLLDD35 pKa = 4.02DD36 pKa = 3.91SEE38 pKa = 5.57AVFDD42 pKa = 4.81DD43 pKa = 4.55QVLTNCEE50 pKa = 3.82YY51 pKa = 10.4MAGEE55 pKa = 4.05FLKK58 pKa = 10.92EE59 pKa = 3.67ILDD62 pKa = 4.34VIDD65 pKa = 4.76EE66 pKa = 4.88GNSSSGSSVQMDD78 pKa = 3.61RR79 pKa = 11.84SEE81 pKa = 4.26SSYY84 pKa = 11.19TDD86 pKa = 3.06VGLSSDD92 pKa = 4.19EE93 pKa = 4.34GLQSDD98 pKa = 4.74YY99 pKa = 11.33SWHH102 pKa = 6.89SDD104 pKa = 2.9MGHH107 pKa = 6.3YY108 pKa = 10.83DD109 pKa = 4.36LDD111 pKa = 4.51GMDD114 pKa = 4.39AVPMAGHH121 pKa = 6.66FEE123 pKa = 4.06EE124 pKa = 5.23FQCNTFDD131 pKa = 4.9IDD133 pKa = 4.91PNTPPQWQDD142 pKa = 1.77VDD144 pKa = 3.5QYY146 pKa = 11.24YY147 pKa = 10.97DD148 pKa = 3.06EE149 pKa = 4.91LMRR152 pKa = 11.84LEE154 pKa = 4.46GEE156 pKa = 4.3EE157 pKa = 3.96NLL159 pKa = 4.6

Molecular weight: 18.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P7MDY6|A0A3P7MDY6_CYLGO Ion_trans domain-containing protein (Fragment) OS=Cylicostephanus goldi OX=71465 GN=CGOC_LOCUS10715 PE=4 SV=1
MM1 pKa = 7.58LRR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.34PFRR8 pKa = 11.84KK9 pKa = 9.42HH10 pKa = 4.75GSIRR14 pKa = 11.84GRR16 pKa = 11.84QLRR19 pKa = 11.84LWLNRR24 pKa = 11.84VSTTRR29 pKa = 11.84SVLLGRR35 pKa = 4.7

Molecular weight: 4.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13786

0

13786

2470701

29

2908

179.2

20.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.182 ± 0.021

2.06 ± 0.017

5.378 ± 0.022

6.808 ± 0.036

4.254 ± 0.018

5.732 ± 0.031

2.361 ± 0.012

5.389 ± 0.017

5.826 ± 0.028

9.05 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.011

4.029 ± 0.015

5.131 ± 0.029

3.879 ± 0.022

5.951 ± 0.024

7.546 ± 0.024

5.515 ± 0.026

6.685 ± 0.024

1.117 ± 0.01

3.095 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski