Pseudomonas phage Waldo5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; unclassified Ghunavirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LJP5|A0A7G8LJP5_9CAUD Uncharacterized protein OS=Pseudomonas phage Waldo5 OX=2762290 PE=4 SV=1
MM1 pKa = 7.37LKK3 pKa = 10.42VIQHH7 pKa = 6.93HH8 pKa = 5.92IQNPDD13 pKa = 4.59DD14 pKa = 4.22IPSIPPASAEE24 pKa = 4.01YY25 pKa = 10.53LAVRR29 pKa = 11.84LNASYY34 pKa = 10.91LIATGVVDD42 pKa = 4.97DD43 pKa = 5.2LRR45 pKa = 11.84KK46 pKa = 9.42QGYY49 pKa = 8.57SEE51 pKa = 5.12GYY53 pKa = 9.22IAGFLDD59 pKa = 3.89GCNAAVEE66 pKa = 4.57ITDD69 pKa = 5.11LMQEE73 pKa = 4.06SQLQKK78 pKa = 11.18EE79 pKa = 4.56EE80 pKa = 4.0

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LJN8|A0A7G8LJN8_9CAUD Uncharacterized protein OS=Pseudomonas phage Waldo5 OX=2762290 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.07ALKK5 pKa = 10.78SFDD8 pKa = 3.28IVTALILLAGRR19 pKa = 11.84IAQRR23 pKa = 11.84RR24 pKa = 11.84HH25 pKa = 4.24QKK27 pKa = 10.08LVARR31 pKa = 11.84EE32 pKa = 4.23ASLKK36 pKa = 10.54AAIAATQRR44 pKa = 11.84AYY46 pKa = 10.99QDD48 pKa = 3.43TVSARR53 pKa = 11.84VAADD57 pKa = 3.28YY58 pKa = 9.93RR59 pKa = 11.84AQDD62 pKa = 3.31ITRR65 pKa = 11.84VKK67 pKa = 10.79

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12666

40

1393

243.6

27.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.64 ± 0.392

0.947 ± 0.174

6.458 ± 0.241

6.695 ± 0.32

3.805 ± 0.198

7.911 ± 0.297

1.99 ± 0.202

5.053 ± 0.241

5.787 ± 0.301

8.108 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.164

3.829 ± 0.212

4.003 ± 0.238

4.35 ± 0.342

5.985 ± 0.243

5.803 ± 0.311

5.692 ± 0.29

6.766 ± 0.367

1.476 ± 0.118

3.119 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski