Amino acid dipepetide frequency for Bosea sp. AAP35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.453AlaAla: 19.453 ± 0.171	1.187AlaCys: 1.187 ± 0.032	6.806AlaAsp: 6.806 ± 0.076	7.366AlaGlu: 7.366 ± 0.091	4.93AlaPhe: 4.93 ± 0.066	11.943AlaGly: 11.943 ± 0.116	2.367AlaHis: 2.367 ± 0.048	7.224AlaIle: 7.224 ± 0.075	4.294AlaLys: 4.294 ± 0.07	15.479AlaLeu: 15.479 ± 0.139	3.912AlaMet: 3.912 ± 0.061	2.612AlaAsn: 2.612 ± 0.05	6.208AlaPro: 6.208 ± 0.101	4.754AlaGln: 4.754 ± 0.064	9.868AlaArg: 9.868 ± 0.097	6.748AlaSer: 6.748 ± 0.076	6.749AlaThr: 6.749 ± 0.078	9.308AlaVal: 9.308 ± 0.095	1.575AlaTrp: 1.575 ± 0.041	2.609AlaTyr: 2.609 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.008CysAla: 1.008 ± 0.029	0.094CysCys: 0.094 ± 0.009	0.511CysAsp: 0.511 ± 0.022	0.446CysGlu: 0.446 ± 0.02	0.333CysPhe: 0.333 ± 0.015	0.926CysGly: 0.926 ± 0.031	0.186CysHis: 0.186 ± 0.014	0.395CysIle: 0.395 ± 0.018	0.181CysLys: 0.181 ± 0.012	0.82CysLeu: 0.82 ± 0.027	0.123CysMet: 0.123 ± 0.011	0.167CysAsn: 0.167 ± 0.014	0.405CysPro: 0.405 ± 0.018	0.217CysGln: 0.217 ± 0.011	0.584CysArg: 0.584 ± 0.024	0.413CysSer: 0.413 ± 0.02	0.369CysThr: 0.369 ± 0.021	0.594CysVal: 0.594 ± 0.026	0.104CysTrp: 0.104 ± 0.01	0.156CysTyr: 0.156 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.078AspAla: 7.078 ± 0.082	0.442AspCys: 0.442 ± 0.02	2.875AspAsp: 2.875 ± 0.054	3.43AspGlu: 3.43 ± 0.05	2.012AspPhe: 2.012 ± 0.042	5.292AspGly: 5.292 ± 0.071	1.218AspHis: 1.218 ± 0.032	2.996AspIle: 2.996 ± 0.051	1.644AspLys: 1.644 ± 0.041	5.999AspLeu: 5.999 ± 0.077	1.207AspMet: 1.207 ± 0.038	1.035AspAsn: 1.035 ± 0.032	3.383AspPro: 3.383 ± 0.06	1.457AspGln: 1.457 ± 0.042	4.192AspArg: 4.192 ± 0.058	2.016AspSer: 2.016 ± 0.041	2.334AspThr: 2.334 ± 0.048	3.968AspVal: 3.968 ± 0.062	0.887AspTrp: 0.887 ± 0.026	1.265AspTyr: 1.265 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.466GluAla: 8.466 ± 0.091	0.309GluCys: 0.309 ± 0.018	2.457GluAsp: 2.457 ± 0.048	2.657GluGlu: 2.657 ± 0.053	1.538GluPhe: 1.538 ± 0.037	4.398GluGly: 4.398 ± 0.067	1.055GluHis: 1.055 ± 0.033	3.578GluIle: 3.578 ± 0.061	1.92GluLys: 1.92 ± 0.049	4.9GluLeu: 4.9 ± 0.066	1.448GluMet: 1.448 ± 0.035	1.236GluAsn: 1.236 ± 0.032	2.964GluPro: 2.964 ± 0.052	1.853GluGln: 1.853 ± 0.044	5.025GluArg: 5.025 ± 0.081	2.309GluSer: 2.309 ± 0.044	3.589GluThr: 3.589 ± 0.066	3.524GluVal: 3.524 ± 0.054	0.619GluTrp: 0.619 ± 0.024	0.717GluTyr: 0.717 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.967PheAla: 4.967 ± 0.068	0.378PheCys: 0.378 ± 0.017	2.463PheAsp: 2.463 ± 0.045	2.066PheGlu: 2.066 ± 0.037	1.364PhePhe: 1.364 ± 0.037	3.742PheGly: 3.742 ± 0.061	0.677PheHis: 0.677 ± 0.024	1.661PheIle: 1.661 ± 0.045	1.065PheLys: 1.065 ± 0.035	3.33PheLeu: 3.33 ± 0.059	0.815PheMet: 0.815 ± 0.022	0.927PheAsn: 0.927 ± 0.03	1.575PhePro: 1.575 ± 0.041	0.958PheGln: 0.958 ± 0.027	2.235PheArg: 2.235 ± 0.049	2.055PheSer: 2.055 ± 0.043	1.919PheThr: 1.919 ± 0.04	2.878PheVal: 2.878 ± 0.05	0.523PheTrp: 0.523 ± 0.022	0.792PheTyr: 0.792 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.122GlyAla: 10.122 ± 0.105	0.867GlyCys: 0.867 ± 0.028	4.393GlyAsp: 4.393 ± 0.074	5.083GlyGlu: 5.083 ± 0.062	3.856GlyPhe: 3.856 ± 0.06	7.686GlyGly: 7.686 ± 0.102	1.994GlyHis: 1.994 ± 0.045	4.624GlyIle: 4.624 ± 0.064	3.142GlyLys: 3.142 ± 0.058	10.028GlyLeu: 10.028 ± 0.108	2.332GlyMet: 2.332 ± 0.045	1.893GlyAsn: 1.893 ± 0.053	3.891GlyPro: 3.891 ± 0.063	3.037GlyGln: 3.037 ± 0.057	6.694GlyArg: 6.694 ± 0.088	4.554GlySer: 4.554 ± 0.07	4.499GlyThr: 4.499 ± 0.066	6.24GlyVal: 6.24 ± 0.068	1.391GlyTrp: 1.391 ± 0.034	2.143GlyTyr: 2.143 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.436HisAla: 2.436 ± 0.044	0.208HisCys: 0.208 ± 0.015	1.246HisAsp: 1.246 ± 0.032	1.044HisGlu: 1.044 ± 0.029	0.751HisPhe: 0.751 ± 0.026	2.011HisGly: 2.011 ± 0.041	0.555HisHis: 0.555 ± 0.022	0.852HisIle: 0.852 ± 0.028	0.465HisLys: 0.465 ± 0.018	2.082HisLeu: 2.082 ± 0.044	0.413HisMet: 0.413 ± 0.02	0.377HisAsn: 0.377 ± 0.018	1.255HisPro: 1.255 ± 0.035	0.536HisGln: 0.536 ± 0.023	1.408HisArg: 1.408 ± 0.036	0.809HisSer: 0.809 ± 0.029	0.661HisThr: 0.661 ± 0.021	1.541HisVal: 1.541 ± 0.032	0.328HisTrp: 0.328 ± 0.014	0.468HisTyr: 0.468 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	8.176IleAla: 8.176 ± 0.084	0.528IleCys: 0.528 ± 0.021	3.672IleAsp: 3.672 ± 0.049	3.569IleGlu: 3.569 ± 0.057	1.619IlePhe: 1.619 ± 0.037	5.354IleGly: 5.354 ± 0.065	0.889IleHis: 0.889 ± 0.027	2.251IleIle: 2.251 ± 0.052	1.448IleLys: 1.448 ± 0.042	4.595IleLeu: 4.595 ± 0.061	1.0IleMet: 1.0 ± 0.03	1.157IleAsn: 1.157 ± 0.034	2.28IlePro: 2.28 ± 0.047	1.095IleGln: 1.095 ± 0.034	3.227IleArg: 3.227 ± 0.047	2.477IleSer: 2.477 ± 0.047	2.62IleThr: 2.62 ± 0.045	4.714IleVal: 4.714 ± 0.075	0.642IleTrp: 0.642 ± 0.023	1.013IleTyr: 1.013 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.56LysAla: 4.56 ± 0.068	0.124LysCys: 0.124 ± 0.01	1.614LysAsp: 1.614 ± 0.045	1.355LysGlu: 1.355 ± 0.035	0.771LysPhe: 0.771 ± 0.029	2.64LysGly: 2.64 ± 0.053	0.552LysHis: 0.552 ± 0.024	1.636LysIle: 1.636 ± 0.04	1.058LysLys: 1.058 ± 0.04	3.454LysLeu: 3.454 ± 0.055	0.609LysMet: 0.609 ± 0.022	0.698LysAsn: 0.698 ± 0.028	2.189LysPro: 2.189 ± 0.051	0.889LysGln: 0.889 ± 0.028	2.315LysArg: 2.315 ± 0.044	1.621LysSer: 1.621 ± 0.037	1.866LysThr: 1.866 ± 0.045	2.327LysVal: 2.327 ± 0.053	0.311LysTrp: 0.311 ± 0.016	0.455LysTyr: 0.455 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	15.674LeuAla: 15.674 ± 0.163	0.867LeuCys: 0.867 ± 0.026	6.258LeuAsp: 6.258 ± 0.085	4.913LeuGlu: 4.913 ± 0.059	3.569LeuPhe: 3.569 ± 0.064	9.013LeuGly: 9.013 ± 0.11	1.744LeuHis: 1.744 ± 0.042	5.315LeuIle: 5.315 ± 0.076	3.586LeuLys: 3.586 ± 0.059	9.866LeuLeu: 9.866 ± 0.13	2.417LeuMet: 2.417 ± 0.041	2.321LeuAsn: 2.321 ± 0.043	5.99LeuPro: 5.99 ± 0.073	2.703LeuGln: 2.703 ± 0.048	7.016LeuArg: 7.016 ± 0.096	6.421LeuSer: 6.421 ± 0.079	5.752LeuThr: 5.752 ± 0.062	8.353LeuVal: 8.353 ± 0.097	1.252LeuTrp: 1.252 ± 0.034	1.896LeuTyr: 1.896 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	3.371MetAla: 3.371 ± 0.054	0.136MetCys: 0.136 ± 0.011	1.029MetAsp: 1.029 ± 0.031	0.985MetGlu: 0.985 ± 0.027	0.626MetPhe: 0.626 ± 0.025	1.831MetGly: 1.831 ± 0.041	0.403MetHis: 0.403 ± 0.019	1.329MetIle: 1.329 ± 0.037	0.922MetLys: 0.922 ± 0.024	2.647MetLeu: 2.647 ± 0.05	0.651MetMet: 0.651 ± 0.024	0.704MetAsn: 0.704 ± 0.024	1.555MetPro: 1.555 ± 0.04	0.768MetGln: 0.768 ± 0.025	1.769MetArg: 1.769 ± 0.038	1.654MetSer: 1.654 ± 0.037	1.896MetThr: 1.896 ± 0.04	1.734MetVal: 1.734 ± 0.034	0.177MetTrp: 0.177 ± 0.012	0.248MetTyr: 0.248 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.967AsnAla: 2.967 ± 0.057	0.195AsnCys: 0.195 ± 0.013	1.132AsnAsp: 1.132 ± 0.037	1.044AsnGlu: 1.044 ± 0.029	0.77AsnPhe: 0.77 ± 0.027	1.956AsnGly: 1.956 ± 0.047	0.427AsnHis: 0.427 ± 0.019	1.1AsnIle: 1.1 ± 0.031	0.583AsnLys: 0.583 ± 0.026	2.355AsnLeu: 2.355 ± 0.04	0.494AsnMet: 0.494 ± 0.021	0.517AsnAsn: 0.517 ± 0.023	1.64AsnPro: 1.64 ± 0.043	0.648AsnGln: 0.648 ± 0.024	1.618AsnArg: 1.618 ± 0.039	0.981AsnSer: 0.981 ± 0.031	1.056AsnThr: 1.056 ± 0.03	1.682AsnVal: 1.682 ± 0.04	0.377AsnTrp: 0.377 ± 0.018	0.528AsnTyr: 0.528 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	7.155ProAla: 7.155 ± 0.094	0.313ProCys: 0.313 ± 0.015	3.521ProAsp: 3.521 ± 0.056	3.478ProGlu: 3.478 ± 0.065	2.047ProPhe: 2.047 ± 0.047	4.925ProGly: 4.925 ± 0.076	1.087ProHis: 1.087 ± 0.031	2.393ProIle: 2.393 ± 0.049	1.564ProLys: 1.564 ± 0.039	5.108ProLeu: 5.108 ± 0.076	1.235ProMet: 1.235 ± 0.032	1.146ProAsn: 1.146 ± 0.032	2.726ProPro: 2.726 ± 0.075	1.787ProGln: 1.787 ± 0.043	3.34ProArg: 3.34 ± 0.061	2.757ProSer: 2.757 ± 0.049	2.525ProThr: 2.525 ± 0.048	4.484ProVal: 4.484 ± 0.056	0.688ProTrp: 0.688 ± 0.024	1.089ProTyr: 1.089 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.773GlnAla: 4.773 ± 0.081	0.181GlnCys: 0.181 ± 0.013	1.447GlnAsp: 1.447 ± 0.034	1.372GlnGlu: 1.372 ± 0.032	0.948GlnPhe: 0.948 ± 0.03	2.528GlnGly: 2.528 ± 0.047	0.593GlnHis: 0.593 ± 0.023	1.894GlnIle: 1.894 ± 0.044	0.921GlnLys: 0.921 ± 0.027	2.604GlnLeu: 2.604 ± 0.048	0.842GlnMet: 0.842 ± 0.026	0.735GlnAsn: 0.735 ± 0.027	1.837GlnPro: 1.837 ± 0.045	1.122GlnGln: 1.122 ± 0.038	2.638GlnArg: 2.638 ± 0.049	1.655GlnSer: 1.655 ± 0.042	1.707GlnThr: 1.707 ± 0.036	2.146GlnVal: 2.146 ± 0.044	0.318GlnTrp: 0.318 ± 0.016	0.494GlnTyr: 0.494 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.539ArgAla: 8.539 ± 0.1	0.491ArgCys: 0.491 ± 0.021	4.21ArgAsp: 4.21 ± 0.062	4.41ArgGlu: 4.41 ± 0.067	3.138ArgPhe: 3.138 ± 0.061	5.091ArgGly: 5.091 ± 0.066	1.653ArgHis: 1.653 ± 0.041	4.245ArgIle: 4.245 ± 0.06	2.124ArgLys: 2.124 ± 0.048	8.515ArgLeu: 8.515 ± 0.103	1.867ArgMet: 1.867 ± 0.038	1.634ArgAsn: 1.634 ± 0.042	3.752ArgPro: 3.752 ± 0.064	2.611ArgGln: 2.611 ± 0.048	5.901ArgArg: 5.901 ± 0.091	3.757ArgSer: 3.757 ± 0.059	3.165ArgThr: 3.165 ± 0.053	4.779ArgVal: 4.779 ± 0.063	0.982ArgTrp: 0.982 ± 0.031	1.61ArgTyr: 1.61 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.345SerAla: 6.345 ± 0.078	0.404SerCys: 0.404 ± 0.021	2.758SerAsp: 2.758 ± 0.049	2.676SerGlu: 2.676 ± 0.052	2.189SerPhe: 2.189 ± 0.048	5.352SerGly: 5.352 ± 0.066	1.054SerHis: 1.054 ± 0.028	2.438SerIle: 2.438 ± 0.052	1.384SerLys: 1.384 ± 0.037	5.624SerLeu: 5.624 ± 0.066	1.134SerMet: 1.134 ± 0.033	1.132SerAsn: 1.132 ± 0.031	2.738SerPro: 2.738 ± 0.055	1.628SerGln: 1.628 ± 0.041	3.677SerArg: 3.677 ± 0.056	2.614SerSer: 2.614 ± 0.058	2.471SerThr: 2.471 ± 0.048	3.893SerVal: 3.893 ± 0.062	0.761SerTrp: 0.761 ± 0.028	1.157SerTyr: 1.157 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.299ThrAla: 6.299 ± 0.079	0.378ThrCys: 0.378 ± 0.016	2.576ThrAsp: 2.576 ± 0.051	2.43ThrGlu: 2.43 ± 0.046	1.81ThrPhe: 1.81 ± 0.035	5.16ThrGly: 5.16 ± 0.075	0.952ThrHis: 0.952 ± 0.031	3.0ThrIle: 3.0 ± 0.049	1.469ThrLys: 1.469 ± 0.036	6.11ThrLeu: 6.11 ± 0.07	1.253ThrMet: 1.253 ± 0.033	1.19ThrAsn: 1.19 ± 0.03	3.302ThrPro: 3.302 ± 0.049	1.624ThrGln: 1.624 ± 0.038	3.432ThrArg: 3.432 ± 0.048	2.603ThrSer: 2.603 ± 0.051	2.88ThrThr: 2.88 ± 0.055	4.284ThrVal: 4.284 ± 0.057	0.602ThrTrp: 0.602 ± 0.025	1.076ThrTyr: 1.076 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.305ValAla: 10.305 ± 0.094	0.587ValCys: 0.587 ± 0.024	3.892ValAsp: 3.892 ± 0.052	4.607ValGlu: 4.607 ± 0.069	2.82ValPhe: 2.82 ± 0.048	5.675ValGly: 5.675 ± 0.062	1.326ValHis: 1.326 ± 0.033	4.034ValIle: 4.034 ± 0.057	2.339ValLys: 2.339 ± 0.045	7.609ValLeu: 7.609 ± 0.094	2.01ValMet: 2.01 ± 0.037	1.734ValAsn: 1.734 ± 0.04	3.845ValPro: 3.845 ± 0.055	1.926ValGln: 1.926 ± 0.041	4.743ValArg: 4.743 ± 0.066	4.271ValSer: 4.271 ± 0.063	4.659ValThr: 4.659 ± 0.066	6.342ValVal: 6.342 ± 0.097	0.864ValTrp: 0.864 ± 0.026	1.373ValTyr: 1.373 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.23TrpAla: 1.23 ± 0.036	0.149TrpCys: 0.149 ± 0.012	0.603TrpAsp: 0.603 ± 0.022	0.521TrpGlu: 0.521 ± 0.019	0.524TrpPhe: 0.524 ± 0.023	0.885TrpGly: 0.885 ± 0.029	0.309TrpHis: 0.309 ± 0.015	0.621TrpIle: 0.621 ± 0.024	0.401TrpLys: 0.401 ± 0.022	1.723TrpLeu: 1.723 ± 0.045	0.326TrpMet: 0.326 ± 0.017	0.388TrpAsn: 0.388 ± 0.016	0.763TrpPro: 0.763 ± 0.023	0.541TrpGln: 0.541 ± 0.022	1.159TrpArg: 1.159 ± 0.033	0.827TrpSer: 0.827 ± 0.026	0.775TrpThr: 0.775 ± 0.025	0.709TrpVal: 0.709 ± 0.026	0.238TrpTrp: 0.238 ± 0.014	0.265TrpTyr: 0.265 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.47TyrAla: 2.47 ± 0.046	0.193TyrCys: 0.193 ± 0.011	1.269TyrAsp: 1.269 ± 0.034	1.108TyrGlu: 1.108 ± 0.033	0.774TyrPhe: 0.774 ± 0.023	2.061TyrGly: 2.061 ± 0.043	0.402TyrHis: 0.402 ± 0.018	0.738TyrIle: 0.738 ± 0.025	0.535TyrLys: 0.535 ± 0.021	2.077TyrLeu: 2.077 ± 0.041	0.346TyrMet: 0.346 ± 0.016	0.468TyrAsn: 0.468 ± 0.021	1.072TyrPro: 1.072 ± 0.028	0.601TyrGln: 0.601 ± 0.022	1.633TyrArg: 1.633 ± 0.04	0.889TyrSer: 0.889 ± 0.032	0.936TyrThr: 0.936 ± 0.032	1.465TyrVal: 1.465 ± 0.035	0.318TyrTrp: 0.318 ± 0.017	0.448TyrTyr: 0.448 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3974 proteins (1218785 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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