Clostridium puniceum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5300 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S8TWT2|A0A1S8TWT2_9CLOT Bifunctional transcriptional activator/DNA repair enzyme AdaA OS=Clostridium puniceum OX=29367 GN=adaA_1 PE=4 SV=1
MM1 pKa = 7.47NKK3 pKa = 9.88NIKK6 pKa = 10.09RR7 pKa = 11.84IVAMALAISGISAVVPATNFNLFTTKK33 pKa = 10.27AYY35 pKa = 10.48ASSDD39 pKa = 3.62DD40 pKa = 4.73ADD42 pKa = 4.0TLDD45 pKa = 3.96SLEE48 pKa = 5.42LLDD51 pKa = 5.61EE52 pKa = 5.6DD53 pKa = 5.64DD54 pKa = 6.38DD55 pKa = 6.63SIDD58 pKa = 3.82LYY60 pKa = 11.35EE61 pKa = 5.36DD62 pKa = 3.08NDD64 pKa = 3.82YY65 pKa = 11.63EE66 pKa = 4.48EE67 pKa = 5.94EE68 pKa = 4.06IDD70 pKa = 3.63SDD72 pKa = 3.93EE73 pKa = 4.39VEE75 pKa = 4.51EE76 pKa = 4.74GNTYY80 pKa = 10.12YY81 pKa = 11.18AEE83 pKa = 4.31TSSDD87 pKa = 3.2TVHH90 pKa = 7.65IDD92 pKa = 3.75EE93 pKa = 5.75IDD95 pKa = 3.79GADD98 pKa = 3.52QDD100 pKa = 3.66NVRR103 pKa = 11.84IFKK106 pKa = 8.77GTSEE110 pKa = 4.08TAYY113 pKa = 10.2EE114 pKa = 4.12IGDD117 pKa = 4.75DD118 pKa = 3.71ISLSSDD124 pKa = 2.97TTTLKK129 pKa = 10.29VRR131 pKa = 11.84IYY133 pKa = 10.74EE134 pKa = 4.12EE135 pKa = 5.44AYY137 pKa = 10.73DD138 pKa = 5.75DD139 pKa = 4.92YY140 pKa = 11.97DD141 pKa = 3.84DD142 pKa = 5.0SNYY145 pKa = 10.41NEE147 pKa = 4.35YY148 pKa = 9.82EE149 pKa = 4.14VKK151 pKa = 10.55VEE153 pKa = 4.33YY154 pKa = 10.44IGDD157 pKa = 4.26DD158 pKa = 3.77DD159 pKa = 6.45DD160 pKa = 6.68DD161 pKa = 5.56EE162 pKa = 6.24NDD164 pKa = 3.57EE165 pKa = 4.63DD166 pKa = 4.51TLDD169 pKa = 4.05SLKK172 pKa = 10.91LSNADD177 pKa = 2.86GDD179 pKa = 4.46TIKK182 pKa = 10.77LYY184 pKa = 10.93EE185 pKa = 4.79DD186 pKa = 3.91DD187 pKa = 5.43NYY189 pKa = 11.69DD190 pKa = 3.56EE191 pKa = 5.12EE192 pKa = 6.61ADD194 pKa = 3.52GDD196 pKa = 4.3EE197 pKa = 4.36VKK199 pKa = 10.71EE200 pKa = 4.47GYY202 pKa = 8.54TYY204 pKa = 10.78YY205 pKa = 11.21ARR207 pKa = 11.84TSSDD211 pKa = 2.96EE212 pKa = 4.13VEE214 pKa = 4.75IEE216 pKa = 4.22TEE218 pKa = 4.32GPDD221 pKa = 3.2ASYY224 pKa = 11.82VKK226 pKa = 10.37VFKK229 pKa = 10.13STSDD233 pKa = 3.23SAKK236 pKa = 10.76GIDD239 pKa = 3.84PGDD242 pKa = 4.36SISIAGDD249 pKa = 3.01KK250 pKa = 10.72VLTVRR255 pKa = 11.84IYY257 pKa = 10.98SEE259 pKa = 4.84EE260 pKa = 4.19PDD262 pKa = 3.24SDD264 pKa = 3.17ITYY267 pKa = 10.79DD268 pKa = 4.0EE269 pKa = 5.41DD270 pKa = 5.54DD271 pKa = 4.4DD272 pKa = 5.74VIGEE276 pKa = 3.99YY277 pKa = 10.06TIEE280 pKa = 4.57LEE282 pKa = 4.86YY283 pKa = 11.18DD284 pKa = 4.19DD285 pKa = 6.0EE286 pKa = 6.38DD287 pKa = 3.79LTNTSSEE294 pKa = 4.06IVEE297 pKa = 4.46EE298 pKa = 4.21TTEE301 pKa = 4.13TSVTDD306 pKa = 3.27STVITNVVSTIATANQWVQANGKK329 pKa = 6.47WQYY332 pKa = 11.51NDD334 pKa = 5.29AIGNPIKK341 pKa = 10.98NNWFFDD347 pKa = 3.74RR348 pKa = 11.84NLGKK352 pKa = 10.02SYY354 pKa = 10.99YY355 pKa = 10.1LQADD359 pKa = 3.81GNMATGWLSNNSKK372 pKa = 9.67WYY374 pKa = 10.45YY375 pKa = 10.0LGPDD379 pKa = 3.5GAMKK383 pKa = 9.98TGWQLVDD390 pKa = 4.59GIWYY394 pKa = 9.78YY395 pKa = 11.47LDD397 pKa = 3.54SQGVMASNITIDD409 pKa = 4.04GYY411 pKa = 11.84KK412 pKa = 10.4LGANGAWIKK421 pKa = 10.89

Molecular weight: 47.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S8T0W7|A0A1S8T0W7_9CLOT Peptidase_M78 domain-containing protein OS=Clostridium puniceum OX=29367 GN=CLPUN_51070 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84EE3 pKa = 3.72EE4 pKa = 4.82GNNSRR9 pKa = 11.84RR10 pKa = 11.84PGGRR14 pKa = 11.84MRR16 pKa = 11.84RR17 pKa = 11.84SRR19 pKa = 11.84KK20 pKa = 9.14KK21 pKa = 9.62VCAFCSDD28 pKa = 2.92KK29 pKa = 11.62SEE31 pKa = 4.79FIDD34 pKa = 3.77YY35 pKa = 11.05KK36 pKa = 11.04DD37 pKa = 3.21INKK40 pKa = 9.06LRR42 pKa = 11.84KK43 pKa = 9.21YY44 pKa = 7.83VTEE47 pKa = 4.22RR48 pKa = 11.84GKK50 pKa = 10.15ILPRR54 pKa = 11.84RR55 pKa = 11.84ISGTCAKK62 pKa = 9.87HH63 pKa = 5.43QRR65 pKa = 11.84EE66 pKa = 4.29LTASIKK72 pKa = 9.88RR73 pKa = 11.84ARR75 pKa = 11.84NIALLPFTTEE85 pKa = 3.59

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5300

0

5300

1617317

29

4579

305.2

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.593 ± 0.038

1.2 ± 0.014

5.5 ± 0.029

7.524 ± 0.042

4.364 ± 0.025

6.284 ± 0.033

1.373 ± 0.012

9.932 ± 0.042

9.027 ± 0.039

8.888 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.014

6.666 ± 0.029

2.701 ± 0.018

2.522 ± 0.017

3.154 ± 0.019

6.46 ± 0.032

5.083 ± 0.03

6.119 ± 0.03

0.818 ± 0.012

4.248 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski