Candidatus Macondimonas diazotrophica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Candidatus Macondimonas

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0F980|A0A4Z0F980_9GAMM NAD-dependent epimerase OS=Candidatus Macondimonas diazotrophica OX=2305248 GN=E4680_09365 PE=4 SV=1
MM1 pKa = 7.45SATSVVWAQSAPEE14 pKa = 3.9AASTIIAVQSFASPTLEE31 pKa = 4.06TPVGFGGSWGSVGVGAFGQTLADD54 pKa = 4.13DD55 pKa = 4.46EE56 pKa = 5.28FDD58 pKa = 3.42ASMGVAVGLGDD69 pKa = 3.73PDD71 pKa = 3.91RR72 pKa = 11.84YY73 pKa = 10.6LGAEE77 pKa = 4.09FGVGFSSLTGDD88 pKa = 3.32NSEE91 pKa = 5.36DD92 pKa = 3.56SFGDD96 pKa = 3.19SGGFSFKK103 pKa = 8.52VHH105 pKa = 6.81RR106 pKa = 11.84NLPGYY111 pKa = 9.45SAVSVGVNGFGVWGGAEE128 pKa = 4.22DD129 pKa = 4.95FNDD132 pKa = 3.53PSYY135 pKa = 10.89YY136 pKa = 10.24AAYY139 pKa = 10.69SKK141 pKa = 9.46MVPVGRR147 pKa = 11.84FASIVTIGAGTEE159 pKa = 4.16VFNDD163 pKa = 3.71PDD165 pKa = 4.51DD166 pKa = 4.91GGVNVFGSGALYY178 pKa = 7.55LTPQVALIGEE188 pKa = 4.26YY189 pKa = 9.54TGRR192 pKa = 11.84FVNAAVSVAPYY203 pKa = 9.95SWLPLTVTVGAVNVTEE219 pKa = 5.31RR220 pKa = 11.84FDD222 pKa = 4.69ADD224 pKa = 3.48TEE226 pKa = 4.35LAVSVGFGFNFTT238 pKa = 4.17

Molecular weight: 24.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0FBY2|A0A4Z0FBY2_9GAMM Uncharacterized protein OS=Candidatus Macondimonas diazotrophica OX=2305248 GN=E4680_05210 PE=4 SV=1
MM1 pKa = 6.96GTGVQYY7 pKa = 11.37VEE9 pKa = 4.83VSAEE13 pKa = 3.87RR14 pKa = 11.84AGQRR18 pKa = 11.84LDD20 pKa = 3.51NFLMGQLKK28 pKa = 9.79EE29 pKa = 4.01IPRR32 pKa = 11.84PQIYY36 pKa = 10.28RR37 pKa = 11.84ILRR40 pKa = 11.84KK41 pKa = 10.4GEE43 pKa = 3.56VRR45 pKa = 11.84VNGRR49 pKa = 11.84RR50 pKa = 11.84AKK52 pKa = 10.15PDD54 pKa = 3.31LRR56 pKa = 11.84IQAGDD61 pKa = 3.7RR62 pKa = 11.84VRR64 pKa = 11.84LPPLQRR70 pKa = 11.84APAAEE75 pKa = 4.23VAIPPASLIGEE86 pKa = 3.9IAGRR90 pKa = 11.84ILYY93 pKa = 10.1EE94 pKa = 4.5DD95 pKa = 4.87DD96 pKa = 3.8GLLVVNKK103 pKa = 9.35PAGLAVHH110 pKa = 7.22AGTGVAYY117 pKa = 10.24GLIDD121 pKa = 3.4VLRR124 pKa = 11.84ALRR127 pKa = 11.84PHH129 pKa = 6.56CPEE132 pKa = 4.24LNLVHH137 pKa = 7.07RR138 pKa = 11.84LDD140 pKa = 4.73RR141 pKa = 11.84GTSGCLVATKK151 pKa = 9.57TRR153 pKa = 11.84SDD155 pKa = 3.77LLTLNRR161 pKa = 11.84AFADD165 pKa = 4.01RR166 pKa = 11.84RR167 pKa = 11.84CEE169 pKa = 3.93KK170 pKa = 10.77VYY172 pKa = 10.79LALTQGRR179 pKa = 11.84WGRR182 pKa = 11.84GEE184 pKa = 3.95VVRR187 pKa = 11.84RR188 pKa = 11.84QALDD192 pKa = 3.03IDD194 pKa = 4.0HH195 pKa = 6.85RR196 pKa = 11.84QGGEE200 pKa = 3.53RR201 pKa = 11.84TVRR204 pKa = 11.84VDD206 pKa = 3.25TAHH209 pKa = 6.33GQTAVSRR216 pKa = 11.84FRR218 pKa = 11.84QVEE221 pKa = 4.18RR222 pKa = 11.84FDD224 pKa = 3.7GASLVRR230 pKa = 11.84VAIEE234 pKa = 3.64TGRR237 pKa = 11.84THH239 pKa = 7.18QIRR242 pKa = 11.84VHH244 pKa = 5.99AADD247 pKa = 4.72AGHH250 pKa = 7.33PLAGDD255 pKa = 3.33DD256 pKa = 3.96RR257 pKa = 11.84YY258 pKa = 11.32GDD260 pKa = 3.56RR261 pKa = 11.84VFNRR265 pKa = 11.84QMARR269 pKa = 11.84LGLRR273 pKa = 11.84RR274 pKa = 11.84LFLHH278 pKa = 6.93AAALSLPLDD287 pKa = 3.63GRR289 pKa = 11.84EE290 pKa = 3.97PLQVEE295 pKa = 4.46APLPADD301 pKa = 3.69LSGVLKK307 pKa = 10.72ALRR310 pKa = 11.84GG311 pKa = 3.63

Molecular weight: 34.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2722

0

2722

837426

24

2010

307.7

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.679 ± 0.07

0.999 ± 0.014

5.644 ± 0.037

5.728 ± 0.04

3.408 ± 0.028

8.237 ± 0.044

2.462 ± 0.028

4.836 ± 0.035

2.601 ± 0.047

10.996 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.022

2.495 ± 0.03

5.452 ± 0.033

3.842 ± 0.03

7.776 ± 0.054

5.091 ± 0.031

5.117 ± 0.036

7.292 ± 0.043

1.518 ± 0.024

2.438 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski