Amino acid dipepetide frequency for Methylophilus sp. Leaf416

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.94AlaAla: 9.94 ± 0.149
0.962AlaCys: 0.962 ± 0.04
5.132AlaAsp: 5.132 ± 0.076
6.057AlaGlu: 6.057 ± 0.101
3.551AlaPhe: 3.551 ± 0.071
6.942AlaGly: 6.942 ± 0.118
2.16AlaHis: 2.16 ± 0.052
6.27AlaIle: 6.27 ± 0.096
5.033AlaLys: 5.033 ± 0.085
10.892AlaLeu: 10.892 ± 0.13
3.011AlaMet: 3.011 ± 0.053
3.917AlaAsn: 3.917 ± 0.066
3.393AlaPro: 3.393 ± 0.072
4.706AlaGln: 4.706 ± 0.087
4.596AlaArg: 4.596 ± 0.087
5.777AlaSer: 5.777 ± 0.096
5.079AlaThr: 5.079 ± 0.082
6.36AlaVal: 6.36 ± 0.098
1.274AlaTrp: 1.274 ± 0.045
2.692AlaTyr: 2.692 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.761CysAla: 0.761 ± 0.032
0.115CysCys: 0.115 ± 0.012
0.457CysAsp: 0.457 ± 0.026
0.462CysGlu: 0.462 ± 0.023
0.322CysPhe: 0.322 ± 0.019
0.719CysGly: 0.719 ± 0.034
0.288CysHis: 0.288 ± 0.017
0.495CysIle: 0.495 ± 0.026
0.346CysLys: 0.346 ± 0.021
0.863CysLeu: 0.863 ± 0.035
0.217CysMet: 0.217 ± 0.016
0.307CysAsn: 0.307 ± 0.02
0.414CysPro: 0.414 ± 0.025
0.421CysGln: 0.421 ± 0.025
0.412CysArg: 0.412 ± 0.024
0.509CysSer: 0.509 ± 0.027
0.379CysThr: 0.379 ± 0.023
0.6CysVal: 0.6 ± 0.026
0.094CysTrp: 0.094 ± 0.011
0.231CysTyr: 0.231 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
5.247AspAla: 5.247 ± 0.083
0.441AspCys: 0.441 ± 0.022
2.606AspAsp: 2.606 ± 0.061
3.125AspGlu: 3.125 ± 0.067
2.24AspPhe: 2.24 ± 0.056
3.669AspGly: 3.669 ± 0.078
1.098AspHis: 1.098 ± 0.034
3.812AspIle: 3.812 ± 0.07
2.767AspLys: 2.767 ± 0.061
5.074AspLeu: 5.074 ± 0.082
1.347AspMet: 1.347 ± 0.044
1.901AspAsn: 1.901 ± 0.054
2.206AspPro: 2.206 ± 0.061
2.074AspGln: 2.074 ± 0.044
2.39AspArg: 2.39 ± 0.06
2.828AspSer: 2.828 ± 0.062
2.75AspThr: 2.75 ± 0.061
3.718AspVal: 3.718 ± 0.078
0.919AspTrp: 0.919 ± 0.036
1.841AspTyr: 1.841 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
5.741GluAla: 5.741 ± 0.094
0.384GluCys: 0.384 ± 0.021
2.618GluAsp: 2.618 ± 0.06
3.119GluGlu: 3.119 ± 0.073
2.14GluPhe: 2.14 ± 0.052
3.334GluGly: 3.334 ± 0.062
1.466GluHis: 1.466 ± 0.044
3.855GluIle: 3.855 ± 0.075
3.402GluLys: 3.402 ± 0.069
5.713GluLeu: 5.713 ± 0.097
1.515GluMet: 1.515 ± 0.041
2.335GluAsn: 2.335 ± 0.058
1.997GluPro: 1.997 ± 0.049
3.178GluGln: 3.178 ± 0.058
3.156GluArg: 3.156 ± 0.07
3.317GluSer: 3.317 ± 0.066
3.222GluThr: 3.222 ± 0.062
4.191GluVal: 4.191 ± 0.082
0.745GluTrp: 0.745 ± 0.027
1.59GluTyr: 1.59 ± 0.044
0.0GluXaa: 0.0 ± 0.0
Phe
3.628PheAla: 3.628 ± 0.07
0.392PheCys: 0.392 ± 0.021
2.478PheAsp: 2.478 ± 0.053
2.307PheGlu: 2.307 ± 0.05
1.607PhePhe: 1.607 ± 0.047
3.11PheGly: 3.11 ± 0.06
0.788PheHis: 0.788 ± 0.032
2.341PheIle: 2.341 ± 0.057
2.016PheLys: 2.016 ± 0.048
3.346PheLeu: 3.346 ± 0.076
0.973PheMet: 0.973 ± 0.034
1.832PheAsn: 1.832 ± 0.052
1.456PhePro: 1.456 ± 0.038
1.321PheGln: 1.321 ± 0.044
1.515PheArg: 1.515 ± 0.04
2.896PheSer: 2.896 ± 0.062
2.2PheThr: 2.2 ± 0.054
2.738PheVal: 2.738 ± 0.064
0.501PheTrp: 0.501 ± 0.027
1.308PheTyr: 1.308 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
5.829GlyAla: 5.829 ± 0.097
0.702GlyCys: 0.702 ± 0.032
3.508GlyAsp: 3.508 ± 0.078
4.037GlyGlu: 4.037 ± 0.073
3.2GlyPhe: 3.2 ± 0.072
5.012GlyGly: 5.012 ± 0.103
1.724GlyHis: 1.724 ± 0.043
4.709GlyIle: 4.709 ± 0.074
4.225GlyLys: 4.225 ± 0.079
7.673GlyLeu: 7.673 ± 0.083
2.122GlyMet: 2.122 ± 0.054
2.864GlyAsn: 2.864 ± 0.075
1.68GlyPro: 1.68 ± 0.045
2.988GlyGln: 2.988 ± 0.062
3.239GlyArg: 3.239 ± 0.068
4.095GlySer: 4.095 ± 0.083
3.533GlyThr: 3.533 ± 0.083
5.408GlyVal: 5.408 ± 0.084
1.097GlyTrp: 1.097 ± 0.04
2.457GlyTyr: 2.457 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
2.572HisAla: 2.572 ± 0.069
0.248HisCys: 0.248 ± 0.018
1.195HisAsp: 1.195 ± 0.039
1.253HisGlu: 1.253 ± 0.04
0.994HisPhe: 0.994 ± 0.033
1.81HisGly: 1.81 ± 0.047
0.811HisHis: 0.811 ± 0.031
1.452HisIle: 1.452 ± 0.04
0.924HisLys: 0.924 ± 0.033
2.481HisLeu: 2.481 ± 0.052
0.582HisMet: 0.582 ± 0.028
0.768HisAsn: 0.768 ± 0.03
1.379HisPro: 1.379 ± 0.043
1.14HisGln: 1.14 ± 0.034
1.096HisArg: 1.096 ± 0.039
1.305HisSer: 1.305 ± 0.043
1.25HisThr: 1.25 ± 0.04
1.557HisVal: 1.557 ± 0.043
0.448HisTrp: 0.448 ± 0.026
0.887HisTyr: 0.887 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.732IleAla: 6.732 ± 0.082
0.55IleCys: 0.55 ± 0.024
3.664IleAsp: 3.664 ± 0.071
4.106IleGlu: 4.106 ± 0.076
2.09IlePhe: 2.09 ± 0.057
4.726IleGly: 4.726 ± 0.075
1.29IleHis: 1.29 ± 0.042
3.118IleIle: 3.118 ± 0.08
3.168IleLys: 3.168 ± 0.063
5.272IleLeu: 5.272 ± 0.088
1.24IleMet: 1.24 ± 0.041
2.633IleAsn: 2.633 ± 0.061
2.732IlePro: 2.732 ± 0.057
2.584IleGln: 2.584 ± 0.061
2.989IleArg: 2.989 ± 0.055
4.277IleSer: 4.277 ± 0.075
3.634IleThr: 3.634 ± 0.068
4.1IleVal: 4.1 ± 0.077
0.592IleTrp: 0.592 ± 0.026
1.659IleTyr: 1.659 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.876LysAla: 4.876 ± 0.087
0.226LysCys: 0.226 ± 0.017
2.457LysAsp: 2.457 ± 0.06
2.709LysGlu: 2.709 ± 0.06
1.665LysPhe: 1.665 ± 0.043
2.961LysGly: 2.961 ± 0.067
1.248LysHis: 1.248 ± 0.043
2.932LysIle: 2.932 ± 0.061
2.714LysLys: 2.714 ± 0.061
5.445LysLeu: 5.445 ± 0.096
1.242LysMet: 1.242 ± 0.042
2.105LysAsn: 2.105 ± 0.052
2.729LysPro: 2.729 ± 0.068
2.949LysGln: 2.949 ± 0.079
2.613LysArg: 2.613 ± 0.053
2.982LysSer: 2.982 ± 0.067
3.087LysThr: 3.087 ± 0.06
3.779LysVal: 3.779 ± 0.071
0.488LysTrp: 0.488 ± 0.022
1.22LysTyr: 1.22 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
10.398LeuAla: 10.398 ± 0.123
0.909LeuCys: 0.909 ± 0.038
5.579LeuAsp: 5.579 ± 0.087
5.617LeuGlu: 5.617 ± 0.09
3.737LeuPhe: 3.737 ± 0.086
7.245LeuGly: 7.245 ± 0.111
2.449LeuHis: 2.449 ± 0.059
6.13LeuIle: 6.13 ± 0.099
5.621LeuLys: 5.621 ± 0.089
10.933LeuLeu: 10.933 ± 0.159
2.722LeuMet: 2.722 ± 0.062
4.5LeuAsn: 4.5 ± 0.08
5.359LeuPro: 5.359 ± 0.08
5.292LeuGln: 5.292 ± 0.09
5.103LeuArg: 5.103 ± 0.08
7.194LeuSer: 7.194 ± 0.109
5.827LeuThr: 5.827 ± 0.091
6.92LeuVal: 6.92 ± 0.099
1.153LeuTrp: 1.153 ± 0.044
2.493LeuTyr: 2.493 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
2.53MetAla: 2.53 ± 0.057
0.154MetCys: 0.154 ± 0.013
1.322MetAsp: 1.322 ± 0.04
1.162MetGlu: 1.162 ± 0.045
0.879MetPhe: 0.879 ± 0.035
1.654MetGly: 1.654 ± 0.043
0.706MetHis: 0.706 ± 0.029
1.39MetIle: 1.39 ± 0.04
1.255MetLys: 1.255 ± 0.039
3.101MetLeu: 3.101 ± 0.062
0.724MetMet: 0.724 ± 0.032
0.92MetAsn: 0.92 ± 0.032
1.368MetPro: 1.368 ± 0.043
1.783MetGln: 1.783 ± 0.05
1.58MetArg: 1.58 ± 0.044
1.615MetSer: 1.615 ± 0.039
1.571MetThr: 1.571 ± 0.046
1.738MetVal: 1.738 ± 0.045
0.205MetTrp: 0.205 ± 0.015
0.467MetTyr: 0.467 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.918AsnAla: 3.918 ± 0.065
0.317AsnCys: 0.317 ± 0.021
1.989AsnAsp: 1.989 ± 0.049
2.115AsnGlu: 2.115 ± 0.057
1.422AsnPhe: 1.422 ± 0.045
3.043AsnGly: 3.043 ± 0.072
0.964AsnHis: 0.964 ± 0.028
2.743AsnIle: 2.743 ± 0.062
1.934AsnLys: 1.934 ± 0.057
3.852AsnLeu: 3.852 ± 0.086
0.933AsnMet: 0.933 ± 0.028
1.726AsnAsn: 1.726 ± 0.054
2.198AsnPro: 2.198 ± 0.051
2.055AsnGln: 2.055 ± 0.053
1.919AsnArg: 1.919 ± 0.053
2.247AsnSer: 2.247 ± 0.056
2.387AsnThr: 2.387 ± 0.063
2.703AsnVal: 2.703 ± 0.058
0.539AsnTrp: 0.539 ± 0.028
1.141AsnTyr: 1.141 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
4.664ProAla: 4.664 ± 0.091
0.293ProCys: 0.293 ± 0.018
2.62ProAsp: 2.62 ± 0.059
3.244ProGlu: 3.244 ± 0.065
1.736ProPhe: 1.736 ± 0.048
2.974ProGly: 2.974 ± 0.07
1.005ProHis: 1.005 ± 0.033
2.263ProIle: 2.263 ± 0.052
1.964ProLys: 1.964 ± 0.055
4.314ProLeu: 4.314 ± 0.074
1.075ProMet: 1.075 ± 0.035
1.73ProAsn: 1.73 ± 0.044
1.643ProPro: 1.643 ± 0.057
1.975ProGln: 1.975 ± 0.044
1.624ProArg: 1.624 ± 0.045
2.314ProSer: 2.314 ± 0.057
2.205ProThr: 2.205 ± 0.055
3.614ProVal: 3.614 ± 0.064
0.567ProTrp: 0.567 ± 0.025
1.35ProTyr: 1.35 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
5.498GlnAla: 5.498 ± 0.092
0.327GlnCys: 0.327 ± 0.021
2.14GlnAsp: 2.14 ± 0.052
2.27GlnGlu: 2.27 ± 0.05
1.843GlnPhe: 1.843 ± 0.051
3.046GlnGly: 3.046 ± 0.064
1.562GlnHis: 1.562 ± 0.049
2.682GlnIle: 2.682 ± 0.061
2.284GlnLys: 2.284 ± 0.056
5.336GlnLeu: 5.336 ± 0.1
1.132GlnMet: 1.132 ± 0.035
1.654GlnAsn: 1.654 ± 0.048
2.29GlnPro: 2.29 ± 0.055
3.446GlnGln: 3.446 ± 0.087
2.627GlnArg: 2.627 ± 0.068
2.914GlnSer: 2.914 ± 0.061
2.678GlnThr: 2.678 ± 0.058
3.435GlnVal: 3.435 ± 0.071
0.733GlnTrp: 0.733 ± 0.032
1.397GlnTyr: 1.397 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
3.966ArgAla: 3.966 ± 0.075
0.382ArgCys: 0.382 ± 0.022
2.635ArgAsp: 2.635 ± 0.055
3.063ArgGlu: 3.063 ± 0.061
2.141ArgPhe: 2.141 ± 0.057
2.888ArgGly: 2.888 ± 0.071
1.283ArgHis: 1.283 ± 0.042
3.153ArgIle: 3.153 ± 0.069
2.361ArgLys: 2.361 ± 0.058
5.52ArgLeu: 5.52 ± 0.088
1.404ArgMet: 1.404 ± 0.038
1.98ArgAsn: 1.98 ± 0.049
1.894ArgPro: 1.894 ± 0.055
2.478ArgGln: 2.478 ± 0.061
2.373ArgArg: 2.373 ± 0.062
2.557ArgSer: 2.557 ± 0.048
2.272ArgThr: 2.272 ± 0.052
3.436ArgVal: 3.436 ± 0.066
0.799ArgTrp: 0.799 ± 0.03
1.689ArgTyr: 1.689 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
5.707SerAla: 5.707 ± 0.085
0.536SerCys: 0.536 ± 0.027
3.029SerAsp: 3.029 ± 0.063
3.29SerGlu: 3.29 ± 0.07
2.429SerPhe: 2.429 ± 0.056
4.968SerGly: 4.968 ± 0.098
1.501SerHis: 1.501 ± 0.042
3.741SerIle: 3.741 ± 0.068
2.938SerLys: 2.938 ± 0.068
6.643SerLeu: 6.643 ± 0.113
1.605SerMet: 1.605 ± 0.042
2.45SerAsn: 2.45 ± 0.053
2.718SerPro: 2.718 ± 0.058
2.867SerGln: 2.867 ± 0.062
3.026SerArg: 3.026 ± 0.06
3.714SerSer: 3.714 ± 0.077
3.164SerThr: 3.164 ± 0.062
4.234SerVal: 4.234 ± 0.081
0.74SerTrp: 0.74 ± 0.031
1.698SerTyr: 1.698 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
5.09ThrAla: 5.09 ± 0.083
0.383ThrCys: 0.383 ± 0.022
2.798ThrAsp: 2.798 ± 0.064
2.944ThrGlu: 2.944 ± 0.067
2.187ThrPhe: 2.187 ± 0.052
4.44ThrGly: 4.44 ± 0.08
1.33ThrHis: 1.33 ± 0.041
3.128ThrIle: 3.128 ± 0.061
2.118ThrLys: 2.118 ± 0.05
6.528ThrLeu: 6.528 ± 0.109
1.181ThrMet: 1.181 ± 0.036
2.039ThrAsn: 2.039 ± 0.069
3.069ThrPro: 3.069 ± 0.063
2.586ThrGln: 2.586 ± 0.055
2.509ThrArg: 2.509 ± 0.056
3.308ThrSer: 3.308 ± 0.074
3.038ThrThr: 3.038 ± 0.064
3.899ThrVal: 3.899 ± 0.067
0.561ThrTrp: 0.561 ± 0.026
1.501ThrTyr: 1.501 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
6.886ValAla: 6.886 ± 0.097
0.657ValCys: 0.657 ± 0.028
3.733ValAsp: 3.733 ± 0.071
4.085ValGlu: 4.085 ± 0.076
2.714ValPhe: 2.714 ± 0.057
4.6ValGly: 4.6 ± 0.088
1.377ValHis: 1.377 ± 0.044
4.818ValIle: 4.818 ± 0.073
3.577ValLys: 3.577 ± 0.067
7.297ValLeu: 7.297 ± 0.102
2.123ValMet: 2.123 ± 0.054
3.01ValAsn: 3.01 ± 0.071
2.849ValPro: 2.849 ± 0.063
2.732ValGln: 2.732 ± 0.053
3.159ValArg: 3.159 ± 0.067
4.672ValSer: 4.672 ± 0.083
4.291ValThr: 4.291 ± 0.074
5.322ValVal: 5.322 ± 0.085
0.872ValTrp: 0.872 ± 0.03
1.847ValTyr: 1.847 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
1.007TrpAla: 1.007 ± 0.037
0.132TrpCys: 0.132 ± 0.013
0.574TrpAsp: 0.574 ± 0.026
0.529TrpGlu: 0.529 ± 0.023
0.55TrpPhe: 0.55 ± 0.026
0.748TrpGly: 0.748 ± 0.03
0.385TrpHis: 0.385 ± 0.021
0.696TrpIle: 0.696 ± 0.026
0.554TrpLys: 0.554 ± 0.026
1.96TrpLeu: 1.96 ± 0.059
0.363TrpMet: 0.363 ± 0.021
0.458TrpAsn: 0.458 ± 0.024
0.474TrpPro: 0.474 ± 0.025
1.069TrpGln: 1.069 ± 0.038
0.768TrpArg: 0.768 ± 0.029
0.735TrpSer: 0.735 ± 0.03
0.499TrpThr: 0.499 ± 0.023
0.984TrpVal: 0.984 ± 0.034
0.256TrpTrp: 0.256 ± 0.018
0.304TrpTyr: 0.304 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.754TyrAla: 2.754 ± 0.058
0.303TyrCys: 0.303 ± 0.017
1.516TyrAsp: 1.516 ± 0.045
1.525TyrGlu: 1.525 ± 0.044
1.305TyrPhe: 1.305 ± 0.042
2.21TyrGly: 2.21 ± 0.047
0.718TyrHis: 0.718 ± 0.031
1.428TyrIle: 1.428 ± 0.038
1.23TyrLys: 1.23 ± 0.039
2.937TyrLeu: 2.937 ± 0.063
0.611TyrMet: 0.611 ± 0.024
1.016TyrAsn: 1.016 ± 0.036
1.328TyrPro: 1.328 ± 0.041
1.649TyrGln: 1.649 ± 0.041
1.581TyrArg: 1.581 ± 0.041
1.742TyrSer: 1.742 ± 0.044
1.569TyrThr: 1.569 ± 0.047
1.887TyrVal: 1.887 ± 0.05
0.463TyrTrp: 0.463 ± 0.021
0.908TyrTyr: 0.908 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2672 proteins (862096 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski