Thermus phage YS40_Isch

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IE40|A0A3T0IE40_9CAUD Deoxycytidylate deaminase OS=Thermus phage YS40_Isch OX=2494333 GN=PGDDIFCJ_00042 PE=3 SV=1
MM1 pKa = 7.81AICYY5 pKa = 9.62IGQEE9 pKa = 4.41EE10 pKa = 5.67VYY12 pKa = 9.44STPSHH17 pKa = 6.56NDD19 pKa = 2.77RR20 pKa = 11.84LNILDD25 pKa = 4.35AEE27 pKa = 4.51SSEE30 pKa = 4.66SLSDD34 pKa = 3.53DD35 pKa = 3.75LNFIRR40 pKa = 11.84SVIFNILGVDD50 pKa = 4.07SGDD53 pKa = 5.45ANNKK57 pKa = 6.78WYY59 pKa = 10.31KK60 pKa = 10.0VPFYY64 pKa = 11.19NLTFLFDD71 pKa = 3.85KK72 pKa = 11.16YY73 pKa = 11.56SLLEE77 pKa = 3.85SDD79 pKa = 5.56FSVEE83 pKa = 4.65HH84 pKa = 5.29YY85 pKa = 9.21TSSEE89 pKa = 4.01DD90 pKa = 3.24PTNAGKK96 pKa = 9.98HH97 pKa = 5.25KK98 pKa = 10.73NVTATGNLNVSGYY111 pKa = 11.21GSIGQNLSVGSYY123 pKa = 10.6LDD125 pKa = 3.25VGTTLTTGSDD135 pKa = 3.12ATIGGNLTVNLSGSILIDD153 pKa = 3.59LSVGRR158 pKa = 11.84DD159 pKa = 3.43LSVGRR164 pKa = 11.84DD165 pKa = 3.5TQVGNNLSVNNNATIVNDD183 pKa = 3.61TSVGGNLTVGGFGTFGSYY201 pKa = 10.35ISVQGDD207 pKa = 3.37ATIQGATSVGGTLTVTGATTLQNNLSVSGNTDD239 pKa = 2.18ISGTLSVLSNATFSANSTVSGNLTVNGKK267 pKa = 7.94TSTSSLYY274 pKa = 10.46VGKK277 pKa = 10.41QYY279 pKa = 10.92IEE281 pKa = 4.1LSGTANVTNN290 pKa = 4.27

Molecular weight: 30.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R5M1|A0A3Q9R5M1_9CAUD Uncharacterized protein OS=Thermus phage YS40_Isch OX=2494333 GN=PGDDIFCJ_00065 PE=4 SV=1
MM1 pKa = 7.42NNLGHH6 pKa = 6.98FLRR9 pKa = 11.84FACWKK14 pKa = 8.68TIRR17 pKa = 11.84LRR19 pKa = 11.84GWTLIASDD27 pKa = 3.72KK28 pKa = 10.47RR29 pKa = 11.84GGRR32 pKa = 11.84IVLILPSFDD41 pKa = 3.51VVEE44 pKa = 4.46GG45 pKa = 3.69

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

175

0

175

50720

33

1762

289.8

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.55 ± 0.173

0.519 ± 0.06

5.398 ± 0.108

8.281 ± 0.333

6.491 ± 0.16

4.614 ± 0.181

1.169 ± 0.062

7.78 ± 0.159

8.764 ± 0.277

9.795 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.471 ± 0.083

6.485 ± 0.194

3.293 ± 0.103

2.677 ± 0.113

3.843 ± 0.141

7.545 ± 0.203

5.008 ± 0.175

6.303 ± 0.11

0.639 ± 0.058

5.377 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski