Streptomyces phage Omar

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BLS9|A0A2H5BLS9_9CAUD ThyX-like thymidylate synthase OS=Streptomyces phage Omar OX=2059882 GN=SEA_OMAR_57 PE=3 SV=1
MM1 pKa = 7.41EE2 pKa = 6.41LDD4 pKa = 4.21RR5 pKa = 11.84DD6 pKa = 4.14TLEE9 pKa = 4.14QAAEE13 pKa = 3.9EE14 pKa = 4.17ALARR18 pKa = 11.84PSDD21 pKa = 3.53AMFFDD26 pKa = 4.79DD27 pKa = 6.26RR28 pKa = 11.84LFTTHH33 pKa = 6.65GAVFHH38 pKa = 5.74WAEE41 pKa = 4.12YY42 pKa = 11.06GDD44 pKa = 4.72DD45 pKa = 4.64LLEE48 pKa = 4.23EE49 pKa = 4.62SNYY52 pKa = 8.88LTALDD57 pKa = 5.31LIRR60 pKa = 11.84GAAGDD65 pKa = 3.73NADD68 pKa = 3.29VHH70 pKa = 7.36VIDD73 pKa = 3.78GTSRR77 pKa = 11.84HH78 pKa = 5.53FACGSLRR85 pKa = 11.84TIYY88 pKa = 9.67VQVYY92 pKa = 8.86EE93 pKa = 4.37SYY95 pKa = 10.79EE96 pKa = 3.87DD97 pKa = 4.15HH98 pKa = 7.04EE99 pKa = 5.22CEE101 pKa = 5.8CEE103 pKa = 4.1PTWEE107 pKa = 4.35HH108 pKa = 6.65EE109 pKa = 4.36EE110 pKa = 3.95EE111 pKa = 4.41CAQDD115 pKa = 4.29EE116 pKa = 4.7GDD118 pKa = 5.28FYY120 pKa = 11.53CQLYY124 pKa = 10.4CRR126 pKa = 11.84IEE128 pKa = 4.42CDD130 pKa = 4.12GEE132 pKa = 4.1QCLPEE137 pKa = 4.2EE138 pKa = 5.03SEE140 pKa = 3.98FTDD143 pKa = 3.4AFKK146 pKa = 11.06VAAEE150 pKa = 4.2LALSLRR156 pKa = 11.84DD157 pKa = 3.76YY158 pKa = 10.89PILDD162 pKa = 3.32EE163 pKa = 4.26SDD165 pKa = 3.54YY166 pKa = 11.73NEE168 pKa = 4.58RR169 pKa = 11.84EE170 pKa = 3.76WEE172 pKa = 4.11LFEE175 pKa = 4.37KK176 pKa = 9.42TLKK179 pKa = 10.56EE180 pKa = 4.17AVEE183 pKa = 4.11HH184 pKa = 5.87AQRR187 pKa = 11.84EE188 pKa = 4.35YY189 pKa = 11.01TLVDD193 pKa = 2.93TWVDD197 pKa = 3.64DD198 pKa = 3.87EE199 pKa = 5.85AIAALYY205 pKa = 9.89YY206 pKa = 10.27EE207 pKa = 5.19NEE209 pKa = 4.65DD210 pKa = 3.6FTHH213 pKa = 5.08QQSWCRR219 pKa = 11.84AEE221 pKa = 5.06DD222 pKa = 3.61VDD224 pKa = 3.59WDD226 pKa = 4.23VVAEE230 pKa = 4.53EE231 pKa = 3.98YY232 pKa = 10.8RR233 pKa = 11.84EE234 pKa = 4.15ARR236 pKa = 11.84DD237 pKa = 3.34AYY239 pKa = 9.43FLEE242 pKa = 4.91RR243 pKa = 11.84ATEE246 pKa = 4.16VYY248 pKa = 9.45RR249 pKa = 11.84WNVLGYY255 pKa = 10.86NPDD258 pKa = 3.49QLVLFAAA265 pKa = 5.04

Molecular weight: 30.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BLK1|A0A2H5BLK1_9CAUD Uncharacterized protein OS=Streptomyces phage Omar OX=2059882 GN=SEA_OMAR_26 PE=4 SV=1
MM1 pKa = 7.34PRR3 pKa = 11.84WEE5 pKa = 4.4GSDD8 pKa = 3.06RR9 pKa = 11.84RR10 pKa = 11.84SRR12 pKa = 11.84LPADD16 pKa = 3.29WPKK19 pKa = 10.44IRR21 pKa = 11.84LRR23 pKa = 11.84VLRR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 3.54GGQCTALNEE37 pKa = 4.25AGEE40 pKa = 4.38RR41 pKa = 11.84CVEE44 pKa = 4.01VATDD48 pKa = 3.4VDD50 pKa = 4.61HH51 pKa = 7.42IRR53 pKa = 11.84PGDD56 pKa = 3.68DD57 pKa = 2.82HH58 pKa = 9.31SMGNLRR64 pKa = 11.84SLCTWHH70 pKa = 6.85HH71 pKa = 5.32RR72 pKa = 11.84QKK74 pKa = 11.19SSAEE78 pKa = 3.73GGAAKK83 pKa = 10.18AARR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84AQAKK92 pKa = 8.96RR93 pKa = 11.84FSRR96 pKa = 11.84TEE98 pKa = 3.6QHH100 pKa = 6.92PGLLL104 pKa = 3.95

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14864

44

1335

185.8

20.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.242 ± 0.366

0.848 ± 0.161

6.539 ± 0.27

7.051 ± 0.411

3.048 ± 0.185

8.356 ± 0.278

2.086 ± 0.215

4.252 ± 0.289

4.501 ± 0.359

8.228 ± 0.438

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.133

2.705 ± 0.127

4.797 ± 0.234

3.384 ± 0.174

6.593 ± 0.424

5.867 ± 0.295

6.29 ± 0.298

7.246 ± 0.149

1.85 ± 0.125

3.021 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski