Prauserella sp. YIM 121212

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Prauserella; unclassified Prauserella

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318LSR3|A0A318LSR3_9PSEU ABC transporter substrate-binding protein OS=Prauserella sp. YIM 121212 OX=1477506 GN=BA062_14890 PE=4 SV=1
MM1 pKa = 7.56LGIVLLGLVSGCAGEE16 pKa = 4.49DD17 pKa = 3.42LSRR20 pKa = 11.84SNYY23 pKa = 9.25QRR25 pKa = 11.84TTVTAEE31 pKa = 3.83AGSGQGEE38 pKa = 4.7VPSGPITDD46 pKa = 3.91PAFGLSALRR55 pKa = 11.84SVDD58 pKa = 3.29PCALLSGDD66 pKa = 4.11VIGDD70 pKa = 4.27LGTVSDD76 pKa = 4.58EE77 pKa = 4.49PYY79 pKa = 8.8EE80 pKa = 4.38SEE82 pKa = 3.83WGSCRR87 pKa = 11.84VDD89 pKa = 3.32VQDD92 pKa = 5.05AGGKK96 pKa = 7.41TIEE99 pKa = 4.34VGVDD103 pKa = 3.13LGGSPMGSDD112 pKa = 3.18PTGAIEE118 pKa = 4.9GLPFLEE124 pKa = 5.02NKK126 pKa = 10.43LDD128 pKa = 3.73DD129 pKa = 3.87TTCYY133 pKa = 9.42STAIYY138 pKa = 9.65SQQPPLGVTVQTGYY152 pKa = 11.1EE153 pKa = 4.16GGNPCDD159 pKa = 3.23VGYY162 pKa = 7.22TTLQQVVRR170 pKa = 11.84ALRR173 pKa = 11.84AEE175 pKa = 3.93PAKK178 pKa = 10.64YY179 pKa = 9.82DD180 pKa = 3.66VPAGSLLEE188 pKa = 4.53ADD190 pKa = 4.2PCASADD196 pKa = 3.76EE197 pKa = 4.7GVLAQVLGGSPSRR210 pKa = 11.84MAMGLHH216 pKa = 6.36ACDD219 pKa = 4.65LSFGTDD225 pKa = 3.16PTISVRR231 pKa = 11.84FRR233 pKa = 11.84AGYY236 pKa = 9.3PPDD239 pKa = 3.67TSDD242 pKa = 4.18GGTEE246 pKa = 3.61VDD248 pKa = 3.81LGGGVKK254 pKa = 10.43AIQEE258 pKa = 4.32PGSTDD263 pKa = 3.09TAEE266 pKa = 5.01CSVEE270 pKa = 4.12WVHH273 pKa = 7.27LPGEE277 pKa = 4.36DD278 pKa = 4.74DD279 pKa = 4.15ANEE282 pKa = 4.14LATVDD287 pKa = 3.86YY288 pKa = 11.35YY289 pKa = 11.43DD290 pKa = 4.5YY291 pKa = 11.74NDD293 pKa = 4.3GASKK297 pKa = 11.06DD298 pKa = 3.98DD299 pKa = 3.42ACARR303 pKa = 11.84ALEE306 pKa = 4.45VAKK309 pKa = 10.7SVAPKK314 pKa = 10.36LPKK317 pKa = 10.27AA318 pKa = 3.96

Molecular weight: 32.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318LUH6|A0A318LUH6_9PSEU Betaine-aldehyde dehydrogenase OS=Prauserella sp. YIM 121212 OX=1477506 GN=BA062_11470 PE=3 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84RR3 pKa = 11.84TRR5 pKa = 11.84TSATWIGVIVFTAVLVLLLIFILQNTQSVQISYY38 pKa = 10.49FGATGHH44 pKa = 6.08VSLAVAMLLAAVAGVLLTAIAGSLRR69 pKa = 11.84IWQLRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84LRR78 pKa = 11.84GVTRR82 pKa = 11.84RR83 pKa = 3.58

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7493

0

7493

2375979

29

8675

317.1

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.319 ± 0.041

0.77 ± 0.008

5.881 ± 0.024

6.058 ± 0.025

2.874 ± 0.015

9.437 ± 0.028

2.207 ± 0.017

3.423 ± 0.021

1.912 ± 0.018

10.719 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.678 ± 0.012

1.803 ± 0.013

5.83 ± 0.026

2.646 ± 0.016

8.058 ± 0.032

4.965 ± 0.016

5.929 ± 0.02

9.016 ± 0.028

1.473 ± 0.011

2.003 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski