Amino acid dipepetide frequency for Flavobacteriaceae bacterium PRS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.835AlaAla: 3.835 ± 0.094	0.581AlaCys: 0.581 ± 0.036	2.922AlaAsp: 2.922 ± 0.079	3.64AlaGlu: 3.64 ± 0.08	3.234AlaPhe: 3.234 ± 0.066	3.744AlaGly: 3.744 ± 0.089	1.013AlaHis: 1.013 ± 0.039	5.822AlaIle: 5.822 ± 0.103	4.691AlaLys: 4.691 ± 0.106	5.819AlaLeu: 5.819 ± 0.111	1.478AlaMet: 1.478 ± 0.06	3.193AlaAsn: 3.193 ± 0.064	1.677AlaPro: 1.677 ± 0.057	2.095AlaGln: 2.095 ± 0.05	1.932AlaArg: 1.932 ± 0.064	3.948AlaSer: 3.948 ± 0.075	3.454AlaThr: 3.454 ± 0.074	3.851AlaVal: 3.851 ± 0.074	0.568AlaTrp: 0.568 ± 0.031	2.298AlaTyr: 2.298 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.463CysAla: 0.463 ± 0.031	0.105CysCys: 0.105 ± 0.013	0.481CysAsp: 0.481 ± 0.029	0.469CysGlu: 0.469 ± 0.026	0.431CysPhe: 0.431 ± 0.028	0.666CysGly: 0.666 ± 0.038	0.176CysHis: 0.176 ± 0.019	0.686CysIle: 0.686 ± 0.033	0.584CysLys: 0.584 ± 0.029	0.609CysLeu: 0.609 ± 0.027	0.157CysMet: 0.157 ± 0.014	0.469CysAsn: 0.469 ± 0.03	0.348CysPro: 0.348 ± 0.031	0.216CysGln: 0.216 ± 0.017	0.23CysArg: 0.23 ± 0.02	0.551CysSer: 0.551 ± 0.035	0.412CysThr: 0.412 ± 0.027	0.456CysVal: 0.456 ± 0.025	0.079CysTrp: 0.079 ± 0.011	0.267CysTyr: 0.267 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.554AspAla: 3.554 ± 0.063	0.443AspCys: 0.443 ± 0.032	3.089AspAsp: 3.089 ± 0.075	3.69AspGlu: 3.69 ± 0.079	3.572AspPhe: 3.572 ± 0.082	3.661AspGly: 3.661 ± 0.102	0.772AspHis: 0.772 ± 0.032	5.233AspIle: 5.233 ± 0.099	4.294AspLys: 4.294 ± 0.084	5.061AspLeu: 5.061 ± 0.096	1.153AspMet: 1.153 ± 0.043	3.306AspAsn: 3.306 ± 0.089	1.419AspPro: 1.419 ± 0.05	1.107AspGln: 1.107 ± 0.044	1.651AspArg: 1.651 ± 0.051	3.167AspSer: 3.167 ± 0.075	2.779AspThr: 2.779 ± 0.077	3.698AspVal: 3.698 ± 0.095	0.742AspTrp: 0.742 ± 0.036	2.862AspTyr: 2.862 ± 0.074	0.0AspXaa: 0.0 ± 0.0
Glu	4.176GluAla: 4.176 ± 0.094	0.375GluCys: 0.375 ± 0.029	3.79GluAsp: 3.79 ± 0.069	4.573GluGlu: 4.573 ± 0.103	3.056GluPhe: 3.056 ± 0.059	3.627GluGly: 3.627 ± 0.077	1.18GluHis: 1.18 ± 0.04	5.835GluIle: 5.835 ± 0.116	5.227GluLys: 5.227 ± 0.111	6.144GluLeu: 6.144 ± 0.1	1.55GluMet: 1.55 ± 0.048	4.526GluAsn: 4.526 ± 0.088	1.448GluPro: 1.448 ± 0.048	2.142GluGln: 2.142 ± 0.054	2.346GluArg: 2.346 ± 0.07	3.274GluSer: 3.274 ± 0.068	3.821GluThr: 3.821 ± 0.076	4.01GluVal: 4.01 ± 0.085	0.565GluTrp: 0.565 ± 0.027	2.358GluTyr: 2.358 ± 0.072	0.0GluXaa: 0.0 ± 0.0
Phe	2.89PheAla: 2.89 ± 0.071	0.426PheCys: 0.426 ± 0.023	3.19PheAsp: 3.19 ± 0.073	3.34PheGlu: 3.34 ± 0.066	2.732PhePhe: 2.732 ± 0.081	3.882PheGly: 3.882 ± 0.078	0.823PheHis: 0.823 ± 0.036	4.449PheIle: 4.449 ± 0.087	4.191PheLys: 4.191 ± 0.098	4.56PheLeu: 4.56 ± 0.099	1.182PheMet: 1.182 ± 0.044	3.714PheAsn: 3.714 ± 0.086	1.753PhePro: 1.753 ± 0.047	1.483PheGln: 1.483 ± 0.047	1.577PheArg: 1.577 ± 0.046	4.055PheSer: 4.055 ± 0.091	2.977PheThr: 2.977 ± 0.077	3.137PheVal: 3.137 ± 0.075	0.593PheTrp: 0.593 ± 0.029	2.072PheTyr: 2.072 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	3.974GlyAla: 3.974 ± 0.099	0.621GlyCys: 0.621 ± 0.037	3.317GlyAsp: 3.317 ± 0.081	3.486GlyGlu: 3.486 ± 0.072	3.725GlyPhe: 3.725 ± 0.08	4.582GlyGly: 4.582 ± 0.112	1.252GlyHis: 1.252 ± 0.046	5.832GlyIle: 5.832 ± 0.1	5.175GlyLys: 5.175 ± 0.081	5.787GlyLeu: 5.787 ± 0.104	1.612GlyMet: 1.612 ± 0.061	3.793GlyAsn: 3.793 ± 0.092	1.313GlyPro: 1.313 ± 0.045	1.641GlyGln: 1.641 ± 0.051	2.205GlyArg: 2.205 ± 0.055	3.731GlySer: 3.731 ± 0.09	3.761GlyThr: 3.761 ± 0.094	4.157GlyVal: 4.157 ± 0.089	0.778GlyTrp: 0.778 ± 0.032	2.766GlyTyr: 2.766 ± 0.068	0.0GlyXaa: 0.0 ± 0.0
His	0.896HisAla: 0.896 ± 0.036	0.198HisCys: 0.198 ± 0.016	0.857HisAsp: 0.857 ± 0.039	0.902HisGlu: 0.902 ± 0.039	1.192HisPhe: 1.192 ± 0.042	1.051HisGly: 1.051 ± 0.043	0.481HisHis: 0.481 ± 0.03	1.705HisIle: 1.705 ± 0.053	1.37HisLys: 1.37 ± 0.045	1.797HisLeu: 1.797 ± 0.056	0.294HisMet: 0.294 ± 0.02	1.075HisAsn: 1.075 ± 0.042	0.917HisPro: 0.917 ± 0.04	0.707HisGln: 0.707 ± 0.033	0.651HisArg: 0.651 ± 0.031	1.07HisSer: 1.07 ± 0.04	0.997HisThr: 0.997 ± 0.036	0.804HisVal: 0.804 ± 0.034	0.201HisTrp: 0.201 ± 0.017	0.804HisTyr: 0.804 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.794IleAla: 5.794 ± 0.116	0.727IleCys: 0.727 ± 0.032	5.438IleAsp: 5.438 ± 0.088	6.171IleGlu: 6.171 ± 0.105	4.064IlePhe: 4.064 ± 0.082	5.725IleGly: 5.725 ± 0.112	1.444IleHis: 1.444 ± 0.048	7.924IleIle: 7.924 ± 0.148	6.913IleLys: 6.913 ± 0.113	7.9IleLeu: 7.9 ± 0.125	1.601IleMet: 1.601 ± 0.051	5.663IleAsn: 5.663 ± 0.112	3.614IlePro: 3.614 ± 0.072	2.482IleGln: 2.482 ± 0.067	2.689IleArg: 2.689 ± 0.067	6.273IleSer: 6.273 ± 0.098	5.417IleThr: 5.417 ± 0.11	5.451IleVal: 5.451 ± 0.098	0.756IleTrp: 0.756 ± 0.03	3.235IleTyr: 3.235 ± 0.078	0.0IleXaa: 0.0 ± 0.0
Lys	4.911LysAla: 4.911 ± 0.098	0.404LysCys: 0.404 ± 0.025	4.672LysAsp: 4.672 ± 0.093	6.04LysGlu: 6.04 ± 0.111	3.101LysPhe: 3.101 ± 0.074	4.78LysGly: 4.78 ± 0.085	1.571LysHis: 1.571 ± 0.055	6.821LysIle: 6.821 ± 0.114	6.98LysLys: 6.98 ± 0.116	6.836LysLeu: 6.836 ± 0.13	1.893LysMet: 1.893 ± 0.051	5.424LysAsn: 5.424 ± 0.092	2.504LysPro: 2.504 ± 0.063	3.075LysGln: 3.075 ± 0.074	3.213LysArg: 3.213 ± 0.078	4.717LysSer: 4.717 ± 0.09	5.16LysThr: 5.16 ± 0.089	4.656LysVal: 4.656 ± 0.097	0.815LysTrp: 0.815 ± 0.037	3.028LysTyr: 3.028 ± 0.077	0.0LysXaa: 0.0 ± 0.0
Leu	5.237LeuAla: 5.237 ± 0.092	0.701LeuCys: 0.701 ± 0.038	4.92LeuAsp: 4.92 ± 0.095	5.827LeuGlu: 5.827 ± 0.116	4.931LeuPhe: 4.931 ± 0.112	5.781LeuGly: 5.781 ± 0.111	1.59LeuHis: 1.59 ± 0.044	7.932LeuIle: 7.932 ± 0.137	8.158LeuLys: 8.158 ± 0.129	8.532LeuLeu: 8.532 ± 0.14	2.04LeuMet: 2.04 ± 0.057	5.966LeuAsn: 5.966 ± 0.099	3.462LeuPro: 3.462 ± 0.068	3.124LeuGln: 3.124 ± 0.082	3.038LeuArg: 3.038 ± 0.073	6.367LeuSer: 6.367 ± 0.098	4.994LeuThr: 4.994 ± 0.094	5.17LeuVal: 5.17 ± 0.086	0.785LeuTrp: 0.785 ± 0.04	3.059LeuTyr: 3.059 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	1.488MetAla: 1.488 ± 0.05	0.143MetCys: 0.143 ± 0.015	1.087MetAsp: 1.087 ± 0.042	1.23MetGlu: 1.23 ± 0.043	0.994MetPhe: 0.994 ± 0.045	1.312MetGly: 1.312 ± 0.049	0.446MetHis: 0.446 ± 0.026	1.715MetIle: 1.715 ± 0.05	2.164MetLys: 2.164 ± 0.053	2.027MetLeu: 2.027 ± 0.065	0.621MetMet: 0.621 ± 0.036	1.386MetAsn: 1.386 ± 0.045	0.825MetPro: 0.825 ± 0.043	0.828MetGln: 0.828 ± 0.038	0.835MetArg: 0.835 ± 0.039	1.548MetSer: 1.548 ± 0.056	1.142MetThr: 1.142 ± 0.042	1.392MetVal: 1.392 ± 0.053	0.157MetTrp: 0.157 ± 0.014	0.715MetTyr: 0.715 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.763AsnAla: 3.763 ± 0.076	0.514AsnCys: 0.514 ± 0.035	3.468AsnAsp: 3.468 ± 0.083	3.805AsnGlu: 3.805 ± 0.078	3.159AsnPhe: 3.159 ± 0.072	3.994AsnGly: 3.994 ± 0.093	1.032AsnHis: 1.032 ± 0.041	5.679AsnIle: 5.679 ± 0.095	4.717AsnLys: 4.717 ± 0.092	5.482AsnLeu: 5.482 ± 0.094	1.4AsnMet: 1.4 ± 0.043	4.41AsnAsn: 4.41 ± 0.106	2.853AsnPro: 2.853 ± 0.065	2.128AsnGln: 2.128 ± 0.057	2.139AsnArg: 2.139 ± 0.063	3.964AsnSer: 3.964 ± 0.076	3.9AsnThr: 3.9 ± 0.094	3.814AsnVal: 3.814 ± 0.082	0.796AsnTrp: 0.796 ± 0.035	2.964AsnTyr: 2.964 ± 0.061	0.0AsnXaa: 0.0 ± 0.0
Pro	1.58ProAla: 1.58 ± 0.057	0.2ProCys: 0.2 ± 0.017	1.992ProAsp: 1.992 ± 0.063	2.627ProGlu: 2.627 ± 0.062	1.849ProPhe: 1.849 ± 0.052	1.727ProGly: 1.727 ± 0.055	0.657ProHis: 0.657 ± 0.032	2.906ProIle: 2.906 ± 0.064	2.792ProLys: 2.792 ± 0.075	2.882ProLeu: 2.882 ± 0.066	0.717ProMet: 0.717 ± 0.032	2.283ProAsn: 2.283 ± 0.06	0.8ProPro: 0.8 ± 0.04	1.056ProGln: 1.056 ± 0.04	1.004ProArg: 1.004 ± 0.042	2.071ProSer: 2.071 ± 0.056	1.867ProThr: 1.867 ± 0.065	2.114ProVal: 2.114 ± 0.056	0.385ProTrp: 0.385 ± 0.025	1.342ProTyr: 1.342 ± 0.053	0.0ProXaa: 0.0 ± 0.0
Gln	1.715GlnAla: 1.715 ± 0.042	0.175GlnCys: 0.175 ± 0.02	1.816GlnAsp: 1.816 ± 0.046	2.177GlnGlu: 2.177 ± 0.07	1.776GlnPhe: 1.776 ± 0.05	1.737GlnGly: 1.737 ± 0.059	0.667GlnHis: 0.667 ± 0.028	2.794GlnIle: 2.794 ± 0.064	2.508GlnLys: 2.508 ± 0.068	3.315GlnLeu: 3.315 ± 0.074	0.778GlnMet: 0.778 ± 0.035	1.928GlnAsn: 1.928 ± 0.056	1.005GlnPro: 1.005 ± 0.036	1.31GlnGln: 1.31 ± 0.052	1.125GlnArg: 1.125 ± 0.044	1.771GlnSer: 1.771 ± 0.045	1.789GlnThr: 1.789 ± 0.053	1.73GlnVal: 1.73 ± 0.049	0.321GlnTrp: 0.321 ± 0.02	1.218GlnTyr: 1.218 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	1.938ArgAla: 1.938 ± 0.062	0.239ArgCys: 0.239 ± 0.021	1.724ArgAsp: 1.724 ± 0.049	2.155ArgGlu: 2.155 ± 0.057	1.873ArgPhe: 1.873 ± 0.064	2.093ArgGly: 2.093 ± 0.056	0.676ArgHis: 0.676 ± 0.035	3.159ArgIle: 3.159 ± 0.072	2.763ArgLys: 2.763 ± 0.07	3.07ArgLeu: 3.07 ± 0.073	0.818ArgMet: 0.818 ± 0.042	2.059ArgAsn: 2.059 ± 0.059	1.08ArgPro: 1.08 ± 0.044	1.083ArgGln: 1.083 ± 0.04	1.329ArgArg: 1.329 ± 0.047	1.909ArgSer: 1.909 ± 0.054	1.787ArgThr: 1.787 ± 0.055	2.052ArgVal: 2.052 ± 0.052	0.344ArgTrp: 0.344 ± 0.021	1.475ArgTyr: 1.475 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	3.509SerAla: 3.509 ± 0.074	0.648SerCys: 0.648 ± 0.035	3.343SerAsp: 3.343 ± 0.08	4.088SerGlu: 4.088 ± 0.075	3.678SerPhe: 3.678 ± 0.078	4.539SerGly: 4.539 ± 0.091	1.08SerHis: 1.08 ± 0.043	5.731SerIle: 5.731 ± 0.093	5.077SerLys: 5.077 ± 0.09	5.776SerLeu: 5.776 ± 0.111	1.301SerMet: 1.301 ± 0.05	4.038SerAsn: 4.038 ± 0.092	1.973SerPro: 1.973 ± 0.048	2.104SerGln: 2.104 ± 0.057	2.144SerArg: 2.144 ± 0.055	4.246SerSer: 4.246 ± 0.086	3.526SerThr: 3.526 ± 0.087	3.924SerVal: 3.924 ± 0.086	0.78SerTrp: 0.78 ± 0.036	2.719SerTyr: 2.719 ± 0.075	0.0SerXaa: 0.0 ± 0.0
Thr	3.382ThrAla: 3.382 ± 0.085	0.369ThrCys: 0.369 ± 0.024	3.005ThrAsp: 3.005 ± 0.078	3.32ThrGlu: 3.32 ± 0.073	3.133ThrPhe: 3.133 ± 0.074	3.714ThrGly: 3.714 ± 0.076	1.089ThrHis: 1.089 ± 0.045	5.72ThrIle: 5.72 ± 0.107	4.284ThrLys: 4.284 ± 0.085	5.345ThrLeu: 5.345 ± 0.089	1.014ThrMet: 1.014 ± 0.037	3.669ThrAsn: 3.669 ± 0.086	2.251ThrPro: 2.251 ± 0.068	1.759ThrGln: 1.759 ± 0.054	1.714ThrArg: 1.714 ± 0.056	3.809ThrSer: 3.809 ± 0.076	3.481ThrThr: 3.481 ± 0.102	3.684ThrVal: 3.684 ± 0.081	0.574ThrTrp: 0.574 ± 0.034	2.238ThrTyr: 2.238 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	3.818ValAla: 3.818 ± 0.085	0.562ValCys: 0.562 ± 0.028	3.349ValAsp: 3.349 ± 0.069	3.799ValGlu: 3.799 ± 0.085	3.483ValPhe: 3.483 ± 0.079	3.751ValGly: 3.751 ± 0.086	0.978ValHis: 0.978 ± 0.039	5.424ValIle: 5.424 ± 0.091	4.433ValLys: 4.433 ± 0.089	5.947ValLeu: 5.947 ± 0.103	1.339ValMet: 1.339 ± 0.047	3.531ValAsn: 3.531 ± 0.083	1.94ValPro: 1.94 ± 0.061	1.609ValGln: 1.609 ± 0.052	1.928ValArg: 1.928 ± 0.059	4.474ValSer: 4.474 ± 0.092	3.531ValThr: 3.531 ± 0.091	3.945ValVal: 3.945 ± 0.079	0.624ValTrp: 0.624 ± 0.033	2.259ValTyr: 2.259 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.562TrpAla: 0.562 ± 0.032	0.099TrpCys: 0.099 ± 0.011	0.577TrpAsp: 0.577 ± 0.03	0.631TrpGlu: 0.631 ± 0.034	0.59TrpPhe: 0.59 ± 0.033	0.6TrpGly: 0.6 ± 0.032	0.224TrpHis: 0.224 ± 0.018	0.804TrpIle: 0.804 ± 0.038	0.879TrpLys: 0.879 ± 0.039	1.0TrpLeu: 1.0 ± 0.043	0.36TrpMet: 0.36 ± 0.023	0.756TrpAsn: 0.756 ± 0.045	0.261TrpPro: 0.261 ± 0.02	0.373TrpGln: 0.373 ± 0.029	0.37TrpArg: 0.37 ± 0.026	0.656TrpSer: 0.656 ± 0.037	0.548TrpThr: 0.548 ± 0.034	0.571TrpVal: 0.571 ± 0.031	0.143TrpTrp: 0.143 ± 0.016	0.463TrpTyr: 0.463 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.308TyrAla: 2.308 ± 0.057	0.328TyrCys: 0.328 ± 0.023	2.218TyrAsp: 2.218 ± 0.056	2.055TyrGlu: 2.055 ± 0.056	2.455TyrPhe: 2.455 ± 0.064	2.54TyrGly: 2.54 ± 0.069	0.818TyrHis: 0.818 ± 0.034	3.113TyrIle: 3.113 ± 0.071	3.4TyrLys: 3.4 ± 0.079	3.726TyrLeu: 3.726 ± 0.079	0.743TyrMet: 0.743 ± 0.038	2.789TyrAsn: 2.789 ± 0.074	1.408TyrPro: 1.408 ± 0.05	1.333TyrGln: 1.333 ± 0.044	1.504TyrArg: 1.504 ± 0.054	2.627TyrSer: 2.627 ± 0.062	2.212TyrThr: 2.212 ± 0.064	2.103TyrVal: 2.103 ± 0.058	0.463TyrTrp: 0.463 ± 0.03	1.795TyrTyr: 1.795 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2108 proteins (686236 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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