Streptococcus satellite phage Javan398

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLI3|A0A4D5ZLI3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan398 OX=2558678 GN=JavanS398_0007 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.4NLEE5 pKa = 4.17EE6 pKa = 5.44KK7 pKa = 10.32IEE9 pKa = 4.01PVVIILDD16 pKa = 3.82EE17 pKa = 4.39EE18 pKa = 4.81DD19 pKa = 5.15DD20 pKa = 4.17FDD22 pKa = 6.03FEE24 pKa = 4.36EE25 pKa = 4.73TDD27 pKa = 5.58DD28 pKa = 4.88EE29 pKa = 4.43IQAQYY34 pKa = 10.81EE35 pKa = 4.17ADD37 pKa = 3.88CLEE40 pKa = 5.86DD41 pKa = 3.66DD42 pKa = 4.18TQDD45 pKa = 3.43VINWINEE52 pKa = 4.11SNTLAEE58 pKa = 4.29YY59 pKa = 11.21VSMEE63 pKa = 4.26FEE65 pKa = 4.68NMNKK69 pKa = 10.05DD70 pKa = 3.55DD71 pKa = 5.35LGASLEE77 pKa = 4.2NASQCLDD84 pKa = 4.31AIVGMLEE91 pKa = 3.76NGTLVLAGGKK101 pKa = 9.33HH102 pKa = 5.1EE103 pKa = 4.14NN104 pKa = 3.61

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZME2|A0A4D5ZME2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan398 OX=2558678 GN=JavanS398_0013 PE=4 SV=1
MM1 pKa = 7.99EE2 pKa = 4.28ITEE5 pKa = 4.1LQARR9 pKa = 11.84SIRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84QADD17 pKa = 3.48KK18 pKa = 11.26NKK20 pKa = 9.33TSSEE24 pKa = 3.76IAKK27 pKa = 9.95EE28 pKa = 3.91IGISPITYY36 pKa = 9.11RR37 pKa = 11.84KK38 pKa = 9.07VKK40 pKa = 10.28NGSKK44 pKa = 10.38VRR46 pKa = 11.84TSTYY50 pKa = 10.29KK51 pKa = 10.68KK52 pKa = 9.8IMLWLTEE59 pKa = 4.18DD60 pKa = 3.44LL61 pKa = 4.63

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

3291

45

520

164.6

18.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.986 ± 0.995

0.729 ± 0.17

6.077 ± 0.521

8.569 ± 0.821

4.467 ± 0.52

4.284 ± 0.461

1.094 ± 0.21

8.235 ± 0.567

10.301 ± 0.805

9.997 ± 0.709

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.735 ± 0.284

6.047 ± 0.443

2.127 ± 0.22

4.193 ± 0.362

3.525 ± 0.586

5.925 ± 0.477

5.044 ± 0.416

5.378 ± 0.306

0.82 ± 0.183

4.467 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski