[Clostridium] hylemonae DSM 15053

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; [Clostridium] hylemonae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0BYY7|C0BYY7_9FIRM Uncharacterized protein OS=[Clostridium] hylemonae DSM 15053 OX=553973 GN=CLOHYLEM_05026 PE=4 SV=1
MM1 pKa = 6.99MKK3 pKa = 9.02KK4 pKa = 8.45TVALFMTVLMVFSLAACGSGSDD26 pKa = 4.49GKK28 pKa = 11.24GDD30 pKa = 3.7SKK32 pKa = 11.32KK33 pKa = 11.04EE34 pKa = 3.84EE35 pKa = 4.23DD36 pKa = 3.8EE37 pKa = 4.39KK38 pKa = 11.66GGDD41 pKa = 3.66SEE43 pKa = 5.18VVLEE47 pKa = 4.08FQQWFDD53 pKa = 3.43NEE55 pKa = 4.17MEE57 pKa = 4.11EE58 pKa = 6.03GYY60 pKa = 10.12LQSICDD66 pKa = 3.79DD67 pKa = 4.25FYY69 pKa = 11.38EE70 pKa = 4.23EE71 pKa = 3.97TGIKK75 pKa = 9.68IKK77 pKa = 10.76LLSNPYY83 pKa = 10.76ADD85 pKa = 3.69TKK87 pKa = 9.89TQLEE91 pKa = 4.1ASAAAGTMADD101 pKa = 3.68IVALDD106 pKa = 3.81GGWIYY111 pKa = 11.04DD112 pKa = 3.71YY113 pKa = 11.56AKK115 pKa = 10.57QGLLAKK121 pKa = 10.44LDD123 pKa = 3.7GLYY126 pKa = 10.77EE127 pKa = 4.11DD128 pKa = 4.43TGFDD132 pKa = 3.3AKK134 pKa = 10.77SVAEE138 pKa = 4.12GTKK141 pKa = 10.05VDD143 pKa = 3.55KK144 pKa = 10.88ALYY147 pKa = 8.77AQPIVNFPSMMAVNLDD163 pKa = 3.81LLKK166 pKa = 10.98SAGVDD171 pKa = 3.38TLPEE175 pKa = 3.97TWSEE179 pKa = 3.92FQEE182 pKa = 4.53ACEE185 pKa = 4.22KK186 pKa = 10.18VTDD189 pKa = 4.29SGDD192 pKa = 3.35NVYY195 pKa = 10.67GFCSNMSTDD204 pKa = 3.1NATSVEE210 pKa = 4.39YY211 pKa = 9.38LTAFAWNSGCSILTDD226 pKa = 3.8DD227 pKa = 5.14GKK229 pKa = 10.16PFIADD234 pKa = 3.5NEE236 pKa = 4.38VLADD240 pKa = 3.4TCEE243 pKa = 3.98FLKK246 pKa = 11.14GLIDD250 pKa = 4.52AKK252 pKa = 9.89VTSPGIYY259 pKa = 10.11TMTDD263 pKa = 2.72ADD265 pKa = 3.76KK266 pKa = 11.62VEE268 pKa = 4.26EE269 pKa = 4.19FTNGRR274 pKa = 11.84VAFIADD280 pKa = 3.84SVAHH284 pKa = 5.69MSNIRR289 pKa = 11.84EE290 pKa = 4.08QAPDD294 pKa = 3.31MNFTYY299 pKa = 10.65MNMPHH304 pKa = 7.0KK305 pKa = 10.28DD306 pKa = 3.61DD307 pKa = 4.11YY308 pKa = 12.01SGDD311 pKa = 3.46SYY313 pKa = 11.96VRR315 pKa = 11.84VNNWAVGIGEE325 pKa = 3.94NCKK328 pKa = 10.66YY329 pKa = 10.51KK330 pKa = 10.94EE331 pKa = 3.88EE332 pKa = 3.88AAKK335 pKa = 10.41FIEE338 pKa = 4.35YY339 pKa = 10.52LLSPEE344 pKa = 4.27VNADD348 pKa = 3.59LCIHH352 pKa = 6.84ANAFPANSEE361 pKa = 4.36AEE363 pKa = 4.06PQYY366 pKa = 11.35EE367 pKa = 4.28DD368 pKa = 5.35DD369 pKa = 4.21SDD371 pKa = 3.99AFKK374 pKa = 10.83AIYY377 pKa = 9.37DD378 pKa = 3.56VYY380 pKa = 10.47MNSNGVAEE388 pKa = 4.77YY389 pKa = 11.0YY390 pKa = 11.23SMPTAEE396 pKa = 4.86ALMSAFIDD404 pKa = 3.97GLVMYY409 pKa = 10.23FDD411 pKa = 5.03GDD413 pKa = 3.77YY414 pKa = 11.54DD415 pKa = 4.27DD416 pKa = 5.27VNDD419 pKa = 4.4MLSEE423 pKa = 4.15VQTQFDD429 pKa = 3.46AAYY432 pKa = 8.72EE433 pKa = 4.06

Molecular weight: 47.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0C332|C0C332_9FIRM 1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=[Clostridium] hylemonae DSM 15053 OX=553973 GN=dxr PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.72VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3971

0

3971

1201420

35

2254

302.5

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.087 ± 0.041

1.576 ± 0.018

5.585 ± 0.036

7.678 ± 0.049

3.926 ± 0.03

7.785 ± 0.038

1.707 ± 0.018

7.08 ± 0.041

6.396 ± 0.03

8.908 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.221 ± 0.022

3.8 ± 0.024

3.388 ± 0.027

2.98 ± 0.021

4.882 ± 0.038

5.659 ± 0.026

5.325 ± 0.033

7.159 ± 0.032

0.859 ± 0.013

4.0 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski