Amino acid dipepetide frequency for Erwinia phage vB_EamP-S6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.003AlaAla: 12.003 ± 1.382
0.795AlaCys: 0.795 ± 0.222
6.566AlaAsp: 6.566 ± 0.637
6.106AlaGlu: 6.106 ± 0.683
3.011AlaPhe: 3.011 ± 0.326
7.612AlaGly: 7.612 ± 0.676
1.506AlaHis: 1.506 ± 0.229
5.311AlaIle: 5.311 ± 0.497
5.395AlaLys: 5.395 ± 0.515
9.075AlaLeu: 9.075 ± 0.804
2.928AlaMet: 2.928 ± 0.509
4.977AlaAsn: 4.977 ± 0.521
3.764AlaPro: 3.764 ± 0.507
5.353AlaGln: 5.353 ± 0.709
3.764AlaArg: 3.764 ± 0.398
4.433AlaSer: 4.433 ± 0.476
6.566AlaThr: 6.566 ± 0.707
6.064AlaVal: 6.064 ± 0.443
1.087AlaTrp: 1.087 ± 0.187
3.011AlaTyr: 3.011 ± 0.329
0.0AlaXaa: 0.0 ± 0.0
Cys
0.46CysAla: 0.46 ± 0.166
0.042CysCys: 0.042 ± 0.045
0.627CysAsp: 0.627 ± 0.209
0.502CysGlu: 0.502 ± 0.163
0.293CysPhe: 0.293 ± 0.132
0.502CysGly: 0.502 ± 0.186
0.209CysHis: 0.209 ± 0.094
0.502CysIle: 0.502 ± 0.176
0.669CysLys: 0.669 ± 0.186
0.795CysLeu: 0.795 ± 0.213
0.251CysMet: 0.251 ± 0.113
0.251CysAsn: 0.251 ± 0.104
0.376CysPro: 0.376 ± 0.129
0.376CysGln: 0.376 ± 0.142
0.586CysArg: 0.586 ± 0.223
0.711CysSer: 0.711 ± 0.213
0.376CysThr: 0.376 ± 0.125
0.251CysVal: 0.251 ± 0.12
0.167CysTrp: 0.167 ± 0.081
0.502CysTyr: 0.502 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
6.441AspAla: 6.441 ± 0.844
0.586AspCys: 0.586 ± 0.182
3.68AspAsp: 3.68 ± 0.613
3.722AspGlu: 3.722 ± 0.533
2.007AspPhe: 2.007 ± 0.291
4.349AspGly: 4.349 ± 0.48
0.753AspHis: 0.753 ± 0.15
3.137AspIle: 3.137 ± 0.449
3.848AspLys: 3.848 ± 0.393
4.642AspLeu: 4.642 ± 0.447
1.84AspMet: 1.84 ± 0.264
3.178AspAsn: 3.178 ± 0.403
2.969AspPro: 2.969 ± 0.366
2.049AspGln: 2.049 ± 0.296
2.049AspArg: 2.049 ± 0.288
3.806AspSer: 3.806 ± 0.373
3.722AspThr: 3.722 ± 0.445
3.137AspVal: 3.137 ± 0.357
1.129AspTrp: 1.129 ± 0.3
2.593AspTyr: 2.593 ± 0.371
0.0AspXaa: 0.0 ± 0.0
Glu
5.688GluAla: 5.688 ± 0.521
0.46GluCys: 0.46 ± 0.153
2.426GluAsp: 2.426 ± 0.353
3.178GluGlu: 3.178 ± 0.469
3.178GluPhe: 3.178 ± 0.49
3.011GluGly: 3.011 ± 0.293
1.506GluHis: 1.506 ± 0.215
2.677GluIle: 2.677 ± 0.356
2.426GluLys: 2.426 ± 0.277
6.022GluLeu: 6.022 ± 0.547
1.673GluMet: 1.673 ± 0.285
1.966GluAsn: 1.966 ± 0.273
2.3GluPro: 2.3 ± 0.444
3.137GluGln: 3.137 ± 0.326
2.342GluArg: 2.342 ± 0.245
3.722GluSer: 3.722 ± 0.35
3.137GluThr: 3.137 ± 0.369
4.6GluVal: 4.6 ± 0.512
0.753GluTrp: 0.753 ± 0.182
1.673GluTyr: 1.673 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
2.258PheAla: 2.258 ± 0.347
0.418PheCys: 0.418 ± 0.143
2.426PheAsp: 2.426 ± 0.345
1.715PheGlu: 1.715 ± 0.258
0.878PhePhe: 0.878 ± 0.216
2.635PheGly: 2.635 ± 0.457
0.502PheHis: 0.502 ± 0.168
2.426PheIle: 2.426 ± 0.431
2.342PheLys: 2.342 ± 0.258
2.509PheLeu: 2.509 ± 0.375
0.962PheMet: 0.962 ± 0.172
2.342PheAsn: 2.342 ± 0.288
1.255PhePro: 1.255 ± 0.209
1.296PheGln: 1.296 ± 0.177
1.38PheArg: 1.38 ± 0.222
2.551PheSer: 2.551 ± 0.439
2.426PheThr: 2.426 ± 0.305
1.38PheVal: 1.38 ± 0.246
0.669PheTrp: 0.669 ± 0.221
1.255PheTyr: 1.255 ± 0.223
0.0PheXaa: 0.0 ± 0.0
Gly
6.775GlyAla: 6.775 ± 0.601
0.586GlyCys: 0.586 ± 0.177
3.931GlyAsp: 3.931 ± 0.417
4.099GlyGlu: 4.099 ± 0.505
2.467GlyPhe: 2.467 ± 0.29
4.684GlyGly: 4.684 ± 0.591
1.046GlyHis: 1.046 ± 0.235
4.14GlyIle: 4.14 ± 0.317
4.851GlyLys: 4.851 ± 0.547
5.52GlyLeu: 5.52 ± 0.512
1.966GlyMet: 1.966 ± 0.257
3.429GlyAsn: 3.429 ± 0.342
1.506GlyPro: 1.506 ± 0.399
3.137GlyGln: 3.137 ± 0.311
3.262GlyArg: 3.262 ± 0.406
4.684GlySer: 4.684 ± 0.519
5.353GlyThr: 5.353 ± 0.409
4.433GlyVal: 4.433 ± 0.514
1.171GlyTrp: 1.171 ± 0.194
3.889GlyTyr: 3.889 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.171HisAla: 1.171 ± 0.207
0.293HisCys: 0.293 ± 0.131
1.046HisAsp: 1.046 ± 0.171
0.836HisGlu: 0.836 ± 0.174
0.544HisPhe: 0.544 ± 0.166
1.046HisGly: 1.046 ± 0.2
0.627HisHis: 0.627 ± 0.175
0.711HisIle: 0.711 ± 0.138
0.795HisLys: 0.795 ± 0.198
2.133HisLeu: 2.133 ± 0.29
0.711HisMet: 0.711 ± 0.171
0.627HisAsn: 0.627 ± 0.172
1.171HisPro: 1.171 ± 0.233
1.046HisGln: 1.046 ± 0.239
1.087HisArg: 1.087 ± 0.241
0.669HisSer: 0.669 ± 0.168
0.878HisThr: 0.878 ± 0.2
0.962HisVal: 0.962 ± 0.257
0.669HisTrp: 0.669 ± 0.167
0.836HisTyr: 0.836 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
4.391IleAla: 4.391 ± 0.378
0.376IleCys: 0.376 ± 0.145
3.388IleAsp: 3.388 ± 0.429
3.304IleGlu: 3.304 ± 0.444
1.422IlePhe: 1.422 ± 0.214
3.346IleGly: 3.346 ± 0.38
1.296IleHis: 1.296 ± 0.295
2.3IleIle: 2.3 ± 0.374
2.677IleLys: 2.677 ± 0.393
3.931IleLeu: 3.931 ± 0.47
1.296IleMet: 1.296 ± 0.28
2.844IleAsn: 2.844 ± 0.315
2.3IlePro: 2.3 ± 0.329
2.509IleGln: 2.509 ± 0.387
2.844IleArg: 2.844 ± 0.344
3.137IleSer: 3.137 ± 0.423
3.889IleThr: 3.889 ± 0.392
2.969IleVal: 2.969 ± 0.4
0.418IleTrp: 0.418 ± 0.143
1.547IleTyr: 1.547 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
6.022LysAla: 6.022 ± 0.593
0.335LysCys: 0.335 ± 0.126
3.555LysAsp: 3.555 ± 0.442
3.178LysGlu: 3.178 ± 0.323
2.007LysPhe: 2.007 ± 0.282
3.262LysGly: 3.262 ± 0.339
0.92LysHis: 0.92 ± 0.178
1.798LysIle: 1.798 ± 0.281
2.467LysLys: 2.467 ± 0.331
5.855LysLeu: 5.855 ± 0.614
1.924LysMet: 1.924 ± 0.3
3.262LysAsn: 3.262 ± 0.379
1.924LysPro: 1.924 ± 0.284
3.22LysGln: 3.22 ± 0.425
2.3LysArg: 2.3 ± 0.35
3.22LysSer: 3.22 ± 0.348
3.597LysThr: 3.597 ± 0.468
3.471LysVal: 3.471 ± 0.381
0.962LysTrp: 0.962 ± 0.234
1.84LysTyr: 1.84 ± 0.315
0.0LysXaa: 0.0 ± 0.0
Leu
8.992LeuAla: 8.992 ± 0.74
1.004LeuCys: 1.004 ± 0.266
5.771LeuAsp: 5.771 ± 0.511
3.931LeuGlu: 3.931 ± 0.359
2.718LeuPhe: 2.718 ± 0.355
5.437LeuGly: 5.437 ± 0.614
1.547LeuHis: 1.547 ± 0.25
4.6LeuIle: 4.6 ± 0.576
5.144LeuLys: 5.144 ± 0.449
7.444LeuLeu: 7.444 ± 0.732
2.969LeuMet: 2.969 ± 0.312
5.228LeuAsn: 5.228 ± 0.414
4.015LeuPro: 4.015 ± 0.489
4.099LeuGln: 4.099 ± 0.441
4.977LeuArg: 4.977 ± 0.455
5.395LeuSer: 5.395 ± 0.43
6.106LeuThr: 6.106 ± 0.509
6.441LeuVal: 6.441 ± 0.579
0.962LeuTrp: 0.962 ± 0.222
2.677LeuTyr: 2.677 ± 0.318
0.0LeuXaa: 0.0 ± 0.0
Met
4.099MetAla: 4.099 ± 0.452
0.125MetCys: 0.125 ± 0.07
1.046MetAsp: 1.046 ± 0.202
1.171MetGlu: 1.171 ± 0.269
0.669MetPhe: 0.669 ± 0.185
1.798MetGly: 1.798 ± 0.259
0.502MetHis: 0.502 ± 0.146
0.878MetIle: 0.878 ± 0.165
1.715MetLys: 1.715 ± 0.309
2.467MetLeu: 2.467 ± 0.307
0.627MetMet: 0.627 ± 0.165
1.924MetAsn: 1.924 ± 0.273
1.631MetPro: 1.631 ± 0.248
1.589MetGln: 1.589 ± 0.274
1.547MetArg: 1.547 ± 0.253
2.551MetSer: 2.551 ± 0.375
1.798MetThr: 1.798 ± 0.372
1.966MetVal: 1.966 ± 0.252
0.293MetTrp: 0.293 ± 0.108
0.962MetTyr: 0.962 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
5.019AsnAla: 5.019 ± 0.878
0.544AsnCys: 0.544 ± 0.204
2.467AsnAsp: 2.467 ± 0.358
3.053AsnGlu: 3.053 ± 0.364
1.757AsnPhe: 1.757 ± 0.328
3.973AsnGly: 3.973 ± 0.394
1.213AsnHis: 1.213 ± 0.283
2.886AsnIle: 2.886 ± 0.326
3.346AsnLys: 3.346 ± 0.397
5.395AsnLeu: 5.395 ± 0.371
1.506AsnMet: 1.506 ± 0.286
2.342AsnAsn: 2.342 ± 0.284
2.76AsnPro: 2.76 ± 0.312
3.095AsnGln: 3.095 ± 0.395
2.007AsnArg: 2.007 ± 0.256
2.969AsnSer: 2.969 ± 0.361
3.178AsnThr: 3.178 ± 0.446
2.551AsnVal: 2.551 ± 0.325
0.962AsnTrp: 0.962 ± 0.238
1.798AsnTyr: 1.798 ± 0.243
0.0AsnXaa: 0.0 ± 0.0
Pro
4.308ProAla: 4.308 ± 0.552
0.335ProCys: 0.335 ± 0.132
2.928ProAsp: 2.928 ± 0.279
2.844ProGlu: 2.844 ± 0.393
1.589ProPhe: 1.589 ± 0.33
3.22ProGly: 3.22 ± 0.449
0.586ProHis: 0.586 ± 0.211
1.464ProIle: 1.464 ± 0.206
1.966ProLys: 1.966 ± 0.282
3.346ProLeu: 3.346 ± 0.342
1.004ProMet: 1.004 ± 0.176
2.175ProAsn: 2.175 ± 0.414
0.962ProPro: 0.962 ± 0.232
1.757ProGln: 1.757 ± 0.268
1.506ProArg: 1.506 ± 0.256
2.3ProSer: 2.3 ± 0.358
2.76ProThr: 2.76 ± 0.311
3.513ProVal: 3.513 ± 0.311
0.209ProTrp: 0.209 ± 0.11
0.92ProTyr: 0.92 ± 0.178
0.0ProXaa: 0.0 ± 0.0
Gln
5.604GlnAla: 5.604 ± 0.639
0.167GlnCys: 0.167 ± 0.082
2.342GlnAsp: 2.342 ± 0.326
3.137GlnGlu: 3.137 ± 0.413
1.589GlnPhe: 1.589 ± 0.243
2.886GlnGly: 2.886 ± 0.35
0.586GlnHis: 0.586 ± 0.196
2.133GlnIle: 2.133 ± 0.297
2.802GlnLys: 2.802 ± 0.335
4.349GlnLeu: 4.349 ± 0.437
1.547GlnMet: 1.547 ± 0.238
2.677GlnAsn: 2.677 ± 0.459
1.631GlnPro: 1.631 ± 0.265
3.22GlnGln: 3.22 ± 0.475
2.426GlnArg: 2.426 ± 0.343
3.346GlnSer: 3.346 ± 0.515
3.262GlnThr: 3.262 ± 0.359
3.178GlnVal: 3.178 ± 0.447
0.92GlnTrp: 0.92 ± 0.273
1.589GlnTyr: 1.589 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
3.722ArgAla: 3.722 ± 0.44
0.418ArgCys: 0.418 ± 0.159
2.175ArgAsp: 2.175 ± 0.296
2.886ArgGlu: 2.886 ± 0.362
1.924ArgPhe: 1.924 ± 0.283
3.178ArgGly: 3.178 ± 0.394
0.711ArgHis: 0.711 ± 0.166
2.844ArgIle: 2.844 ± 0.412
2.677ArgLys: 2.677 ± 0.413
4.015ArgLeu: 4.015 ± 0.358
1.924ArgMet: 1.924 ± 0.263
2.175ArgAsn: 2.175 ± 0.317
1.422ArgPro: 1.422 ± 0.259
2.049ArgGln: 2.049 ± 0.298
2.593ArgArg: 2.593 ± 0.416
2.3ArgSer: 2.3 ± 0.318
3.262ArgThr: 3.262 ± 0.33
3.137ArgVal: 3.137 ± 0.332
0.669ArgTrp: 0.669 ± 0.242
2.049ArgTyr: 2.049 ± 0.267
0.0ArgXaa: 0.0 ± 0.0
Ser
5.395SerAla: 5.395 ± 0.615
0.418SerCys: 0.418 ± 0.135
3.848SerAsp: 3.848 ± 0.424
2.886SerGlu: 2.886 ± 0.372
1.924SerPhe: 1.924 ± 0.335
5.311SerGly: 5.311 ± 0.624
1.213SerHis: 1.213 ± 0.2
2.969SerIle: 2.969 ± 0.371
2.928SerLys: 2.928 ± 0.274
5.437SerLeu: 5.437 ± 0.481
1.715SerMet: 1.715 ± 0.253
3.304SerAsn: 3.304 ± 0.359
2.342SerPro: 2.342 ± 0.35
2.76SerGln: 2.76 ± 0.496
2.844SerArg: 2.844 ± 0.335
4.349SerSer: 4.349 ± 0.623
3.931SerThr: 3.931 ± 0.478
4.684SerVal: 4.684 ± 0.552
1.004SerTrp: 1.004 ± 0.271
2.175SerTyr: 2.175 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
6.19ThrAla: 6.19 ± 0.603
0.335ThrCys: 0.335 ± 0.158
4.642ThrAsp: 4.642 ± 0.392
3.304ThrGlu: 3.304 ± 0.377
1.798ThrPhe: 1.798 ± 0.266
6.399ThrGly: 6.399 ± 0.553
0.92ThrHis: 0.92 ± 0.197
4.057ThrIle: 4.057 ± 0.45
2.969ThrLys: 2.969 ± 0.425
6.315ThrLeu: 6.315 ± 0.582
1.673ThrMet: 1.673 ± 0.256
3.848ThrAsn: 3.848 ± 0.514
2.844ThrPro: 2.844 ± 0.387
2.467ThrGln: 2.467 ± 0.316
2.467ThrArg: 2.467 ± 0.38
3.68ThrSer: 3.68 ± 0.389
4.224ThrThr: 4.224 ± 0.537
4.684ThrVal: 4.684 ± 0.488
1.087ThrTrp: 1.087 ± 0.33
1.924ThrTyr: 1.924 ± 0.377
0.0ThrXaa: 0.0 ± 0.0
Val
6.524ValAla: 6.524 ± 0.526
0.627ValCys: 0.627 ± 0.197
3.806ValAsp: 3.806 ± 0.359
3.555ValGlu: 3.555 ± 0.426
2.091ValPhe: 2.091 ± 0.39
4.475ValGly: 4.475 ± 0.454
1.129ValHis: 1.129 ± 0.222
2.844ValIle: 2.844 ± 0.387
3.555ValLys: 3.555 ± 0.407
4.684ValLeu: 4.684 ± 0.429
1.589ValMet: 1.589 ± 0.237
3.346ValAsn: 3.346 ± 0.357
3.22ValPro: 3.22 ± 0.374
3.346ValGln: 3.346 ± 0.331
3.513ValArg: 3.513 ± 0.331
5.06ValSer: 5.06 ± 0.454
4.266ValThr: 4.266 ± 0.418
4.349ValVal: 4.349 ± 0.482
1.004ValTrp: 1.004 ± 0.2
2.133ValTyr: 2.133 ± 0.294
0.0ValXaa: 0.0 ± 0.0
Trp
1.464TrpAla: 1.464 ± 0.235
0.209TrpCys: 0.209 ± 0.093
0.836TrpAsp: 0.836 ± 0.212
0.544TrpGlu: 0.544 ± 0.143
0.711TrpPhe: 0.711 ± 0.177
0.962TrpGly: 0.962 ± 0.253
0.418TrpHis: 0.418 ± 0.149
0.544TrpIle: 0.544 ± 0.152
0.753TrpLys: 0.753 ± 0.201
1.924TrpLeu: 1.924 ± 0.384
0.209TrpMet: 0.209 ± 0.096
1.087TrpAsn: 1.087 ± 0.221
0.418TrpPro: 0.418 ± 0.119
0.92TrpGln: 0.92 ± 0.167
0.795TrpArg: 0.795 ± 0.214
0.878TrpSer: 0.878 ± 0.244
0.753TrpThr: 0.753 ± 0.244
0.669TrpVal: 0.669 ± 0.147
0.251TrpTrp: 0.251 ± 0.113
0.586TrpTyr: 0.586 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.802TyrAla: 2.802 ± 0.356
0.376TyrCys: 0.376 ± 0.124
2.133TyrAsp: 2.133 ± 0.25
1.924TyrGlu: 1.924 ± 0.265
1.046TyrPhe: 1.046 ± 0.21
2.844TyrGly: 2.844 ± 0.388
0.669TyrHis: 0.669 ± 0.165
1.966TyrIle: 1.966 ± 0.349
1.798TyrLys: 1.798 ± 0.255
3.638TyrLeu: 3.638 ± 0.475
1.004TyrMet: 1.004 ± 0.215
1.84TyrAsn: 1.84 ± 0.238
0.878TyrPro: 0.878 ± 0.229
1.966TyrGln: 1.966 ± 0.383
1.84TyrArg: 1.84 ± 0.299
1.673TyrSer: 1.673 ± 0.223
2.342TyrThr: 2.342 ± 0.287
2.677TyrVal: 2.677 ± 0.383
0.544TyrTrp: 0.544 ± 0.181
0.878TyrTyr: 0.878 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 115 proteins (23912 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski