Corynebacterium phage phi673

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ikedavirus; Corynebacterium virus phi673

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PIU4|A0A2H4PIU4_9CAUD Uncharacterized protein OS=Corynebacterium phage phi673 OX=2052821 GN=phi673_gp39 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 2.94STATIITTPIARR15 pKa = 11.84DD16 pKa = 3.35ILPIATYY23 pKa = 8.67STQGEE28 pKa = 4.2DD29 pKa = 3.08SKK31 pKa = 11.62LYY33 pKa = 10.37TDD35 pKa = 4.8LFQIAVIQSGIRR47 pKa = 11.84DD48 pKa = 3.82LAILASDD55 pKa = 5.22CIQGKK60 pKa = 9.92DD61 pKa = 3.66DD62 pKa = 5.02SICVLQSTDD71 pKa = 2.48WLQNARR77 pKa = 11.84MGEE80 pKa = 4.03YY81 pKa = 10.27AITIDD86 pKa = 5.1DD87 pKa = 4.27NGSDD91 pKa = 4.88LHH93 pKa = 7.85LEE95 pKa = 3.95WLAGRR100 pKa = 11.84GVGAYY105 pKa = 9.78VNAVNVIGNEE115 pKa = 3.94YY116 pKa = 10.1VEE118 pKa = 5.63AIIKK122 pKa = 9.51VYY124 pKa = 10.0NEE126 pKa = 3.82RR127 pKa = 3.92

Molecular weight: 13.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PIV6|A0A2H4PIV6_9CAUD Putative terminase small subunit OS=Corynebacterium phage phi673 OX=2052821 GN=phi673_gp01 PE=4 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84GQGTQAQLALADD15 pKa = 4.11TLVQDD20 pKa = 4.87LARR23 pKa = 11.84LMRR26 pKa = 11.84GTPVDD31 pKa = 3.7RR32 pKa = 11.84VDD34 pKa = 3.07VASRR38 pKa = 11.84IEE40 pKa = 4.06HH41 pKa = 5.39YY42 pKa = 9.95RR43 pKa = 11.84ALRR46 pKa = 11.84IPRR49 pKa = 11.84YY50 pKa = 7.97EE51 pKa = 3.83SKK53 pKa = 10.94YY54 pKa = 9.26PKK56 pKa = 10.1KK57 pKa = 10.75

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13981

51

1577

249.7

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.521 ± 0.672

0.601 ± 0.133

6.623 ± 0.214

5.715 ± 0.357

2.546 ± 0.173

8.197 ± 0.432

2.332 ± 0.244

4.814 ± 0.235

3.941 ± 0.222

8.004 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.184

3.598 ± 0.27

5.085 ± 0.297

3.855 ± 0.209

5.143 ± 0.412

6.666 ± 0.385

7.61 ± 0.445

7.253 ± 0.212

1.988 ± 0.191

2.747 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski