Amino acid dipepetide frequency for Lactococcus virus ASCC273

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.837AlaAla: 0.837 ± 0.359
0.209AlaCys: 0.209 ± 0.178
2.614AlaAsp: 2.614 ± 0.538
4.706AlaGlu: 4.706 ± 0.833
3.765AlaPhe: 3.765 ± 0.908
4.287AlaGly: 4.287 ± 0.658
0.523AlaHis: 0.523 ± 0.224
4.706AlaIle: 4.706 ± 0.811
5.438AlaLys: 5.438 ± 0.947
5.333AlaLeu: 5.333 ± 0.863
1.987AlaMet: 1.987 ± 0.604
3.66AlaAsn: 3.66 ± 0.761
0.523AlaPro: 0.523 ± 0.23
2.091AlaGln: 2.091 ± 0.487
1.882AlaArg: 1.882 ± 0.407
2.719AlaSer: 2.719 ± 0.642
3.346AlaThr: 3.346 ± 0.677
3.765AlaVal: 3.765 ± 1.247
1.673AlaTrp: 1.673 ± 0.7
2.301AlaTyr: 2.301 ± 0.419
0.0AlaXaa: 0.0 ± 0.0
Cys
0.209CysAla: 0.209 ± 0.158
0.209CysCys: 0.209 ± 0.149
0.209CysAsp: 0.209 ± 0.146
0.314CysGlu: 0.314 ± 0.173
0.314CysPhe: 0.314 ± 0.177
0.837CysGly: 0.837 ± 0.352
0.209CysHis: 0.209 ± 0.139
0.418CysIle: 0.418 ± 0.218
0.837CysLys: 0.837 ± 0.354
0.418CysLeu: 0.418 ± 0.195
0.209CysMet: 0.209 ± 0.138
0.837CysAsn: 0.837 ± 0.347
0.209CysPro: 0.209 ± 0.145
0.209CysGln: 0.209 ± 0.141
0.418CysArg: 0.418 ± 0.196
0.314CysSer: 0.314 ± 0.191
0.209CysThr: 0.209 ± 0.139
0.627CysVal: 0.627 ± 0.305
0.209CysTrp: 0.209 ± 0.168
0.314CysTyr: 0.314 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
1.464AspAla: 1.464 ± 0.424
0.418AspCys: 0.418 ± 0.234
3.451AspAsp: 3.451 ± 0.574
4.706AspGlu: 4.706 ± 0.866
3.765AspPhe: 3.765 ± 0.581
3.974AspGly: 3.974 ± 0.605
1.046AspHis: 1.046 ± 0.382
3.765AspIle: 3.765 ± 0.617
5.647AspLys: 5.647 ± 0.649
6.17AspLeu: 6.17 ± 0.772
1.464AspMet: 1.464 ± 0.404
4.496AspAsn: 4.496 ± 0.596
1.569AspPro: 1.569 ± 0.445
1.046AspGln: 1.046 ± 0.354
1.464AspArg: 1.464 ± 0.504
3.242AspSer: 3.242 ± 0.572
3.974AspThr: 3.974 ± 0.723
3.555AspVal: 3.555 ± 0.566
1.255AspTrp: 1.255 ± 0.333
2.51AspTyr: 2.51 ± 0.432
0.0AspXaa: 0.0 ± 0.0
Glu
3.451GluAla: 3.451 ± 0.57
0.627GluCys: 0.627 ± 0.287
2.928GluAsp: 2.928 ± 0.608
5.333GluGlu: 5.333 ± 1.112
3.346GluPhe: 3.346 ± 0.489
2.301GluGly: 2.301 ± 0.397
0.941GluHis: 0.941 ± 0.333
6.274GluIle: 6.274 ± 0.803
6.274GluLys: 6.274 ± 1.026
10.143GluLeu: 10.143 ± 1.445
2.719GluMet: 2.719 ± 0.655
4.81GluAsn: 4.81 ± 0.573
1.255GluPro: 1.255 ± 0.379
3.974GluGln: 3.974 ± 0.836
2.823GluArg: 2.823 ± 0.556
3.66GluSer: 3.66 ± 0.559
4.706GluThr: 4.706 ± 0.675
4.496GluVal: 4.496 ± 0.64
0.941GluTrp: 0.941 ± 0.305
3.869GluTyr: 3.869 ± 0.799
0.0GluXaa: 0.0 ± 0.0
Phe
2.719PheAla: 2.719 ± 0.577
0.314PheCys: 0.314 ± 0.191
3.451PheAsp: 3.451 ± 0.633
2.614PheGlu: 2.614 ± 0.521
1.987PhePhe: 1.987 ± 0.772
2.405PheGly: 2.405 ± 0.486
0.209PheHis: 0.209 ± 0.137
3.555PheIle: 3.555 ± 0.753
4.078PheLys: 4.078 ± 0.723
2.928PheLeu: 2.928 ± 0.526
0.941PheMet: 0.941 ± 0.318
3.346PheAsn: 3.346 ± 0.857
0.941PhePro: 0.941 ± 0.292
1.15PheGln: 1.15 ± 0.464
1.255PheArg: 1.255 ± 0.342
3.765PheSer: 3.765 ± 1.102
3.137PheThr: 3.137 ± 0.502
2.51PheVal: 2.51 ± 0.457
0.209PheTrp: 0.209 ± 0.139
1.569PheTyr: 1.569 ± 0.351
0.0PheXaa: 0.0 ± 0.0
Gly
3.765GlyAla: 3.765 ± 1.023
0.314GlyCys: 0.314 ± 0.183
3.451GlyAsp: 3.451 ± 0.553
4.078GlyGlu: 4.078 ± 0.478
2.928GlyPhe: 2.928 ± 0.55
4.601GlyGly: 4.601 ± 0.616
0.732GlyHis: 0.732 ± 0.243
3.869GlyIle: 3.869 ± 1.51
5.96GlyLys: 5.96 ± 1.005
5.96GlyLeu: 5.96 ± 0.902
1.882GlyMet: 1.882 ± 0.517
3.555GlyAsn: 3.555 ± 0.568
0.105GlyPro: 0.105 ± 0.101
2.405GlyGln: 2.405 ± 0.484
1.987GlyArg: 1.987 ± 0.346
5.228GlySer: 5.228 ± 0.803
3.765GlyThr: 3.765 ± 0.726
6.379GlyVal: 6.379 ± 1.272
1.359GlyTrp: 1.359 ± 0.313
3.346GlyTyr: 3.346 ± 0.652
0.0GlyXaa: 0.0 ± 0.0
His
0.732HisAla: 0.732 ± 0.242
0.732HisCys: 0.732 ± 0.34
0.837HisAsp: 0.837 ± 0.29
0.627HisGlu: 0.627 ± 0.255
0.314HisPhe: 0.314 ± 0.2
1.15HisGly: 1.15 ± 0.354
0.0HisHis: 0.0 ± 0.0
1.15HisIle: 1.15 ± 0.329
0.627HisLys: 0.627 ± 0.221
0.732HisLeu: 0.732 ± 0.267
0.0HisMet: 0.0 ± 0.0
1.778HisAsn: 1.778 ± 0.427
0.105HisPro: 0.105 ± 0.095
0.314HisGln: 0.314 ± 0.187
0.314HisArg: 0.314 ± 0.202
0.209HisSer: 0.209 ± 0.132
1.15HisThr: 1.15 ± 0.398
0.941HisVal: 0.941 ± 0.368
0.0HisTrp: 0.0 ± 0.0
0.523HisTyr: 0.523 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
3.974IleAla: 3.974 ± 0.48
0.0IleCys: 0.0 ± 0.0
4.392IleAsp: 4.392 ± 0.557
6.588IleGlu: 6.588 ± 1.008
2.719IlePhe: 2.719 ± 0.69
4.183IleGly: 4.183 ± 0.866
1.15IleHis: 1.15 ± 0.307
4.81IleIle: 4.81 ± 0.558
6.588IleLys: 6.588 ± 0.902
5.124IleLeu: 5.124 ± 0.771
2.091IleMet: 2.091 ± 0.474
5.856IleAsn: 5.856 ± 0.623
1.778IlePro: 1.778 ± 0.374
2.51IleGln: 2.51 ± 0.455
1.882IleArg: 1.882 ± 0.427
4.183IleSer: 4.183 ± 0.839
4.601IleThr: 4.601 ± 0.604
4.81IleVal: 4.81 ± 0.827
0.941IleTrp: 0.941 ± 0.392
2.51IleTyr: 2.51 ± 0.551
0.0IleXaa: 0.0 ± 0.0
Lys
6.379LysAla: 6.379 ± 0.89
0.418LysCys: 0.418 ± 0.258
5.333LysAsp: 5.333 ± 0.666
8.156LysGlu: 8.156 ± 1.409
2.51LysPhe: 2.51 ± 0.423
5.96LysGly: 5.96 ± 0.907
1.359LysHis: 1.359 ± 0.455
5.542LysIle: 5.542 ± 0.843
8.993LysLys: 8.993 ± 1.183
7.006LysLeu: 7.006 ± 0.683
2.719LysMet: 2.719 ± 0.494
4.915LysAsn: 4.915 ± 0.647
2.091LysPro: 2.091 ± 0.54
3.765LysGln: 3.765 ± 0.816
3.242LysArg: 3.242 ± 0.71
6.065LysSer: 6.065 ± 0.958
5.019LysThr: 5.019 ± 0.771
5.647LysVal: 5.647 ± 0.747
1.359LysTrp: 1.359 ± 0.292
3.033LysTyr: 3.033 ± 0.563
0.0LysXaa: 0.0 ± 0.0
Leu
4.601LeuAla: 4.601 ± 0.681
0.209LeuCys: 0.209 ± 0.129
5.647LeuAsp: 5.647 ± 0.8
5.542LeuGlu: 5.542 ± 0.855
3.555LeuPhe: 3.555 ± 0.592
4.496LeuGly: 4.496 ± 0.659
1.359LeuHis: 1.359 ± 0.42
7.006LeuIle: 7.006 ± 0.883
8.261LeuLys: 8.261 ± 0.929
6.065LeuLeu: 6.065 ± 1.153
1.569LeuMet: 1.569 ± 0.441
5.438LeuAsn: 5.438 ± 0.77
3.346LeuPro: 3.346 ± 0.69
3.66LeuGln: 3.66 ± 0.595
2.614LeuArg: 2.614 ± 0.473
5.228LeuSer: 5.228 ± 0.677
6.17LeuThr: 6.17 ± 0.749
5.542LeuVal: 5.542 ± 0.737
1.255LeuTrp: 1.255 ± 0.313
4.601LeuTyr: 4.601 ± 0.613
0.0LeuXaa: 0.0 ± 0.0
Met
2.196MetAla: 2.196 ± 0.517
0.105MetCys: 0.105 ± 0.101
1.569MetAsp: 1.569 ± 0.392
1.464MetGlu: 1.464 ± 0.579
0.837MetPhe: 0.837 ± 0.334
1.255MetGly: 1.255 ± 0.29
0.209MetHis: 0.209 ± 0.153
2.301MetIle: 2.301 ± 0.544
3.451MetLys: 3.451 ± 0.77
1.359MetLeu: 1.359 ± 0.408
0.418MetMet: 0.418 ± 0.235
1.569MetAsn: 1.569 ± 0.385
0.627MetPro: 0.627 ± 0.25
1.673MetGln: 1.673 ± 0.368
0.314MetArg: 0.314 ± 0.252
1.987MetSer: 1.987 ± 0.444
1.673MetThr: 1.673 ± 0.466
1.778MetVal: 1.778 ± 0.283
0.0MetTrp: 0.0 ± 0.0
1.046MetTyr: 1.046 ± 0.376
0.0MetXaa: 0.0 ± 0.0
Asn
5.228AsnAla: 5.228 ± 1.15
0.314AsnCys: 0.314 ± 0.201
4.078AsnAsp: 4.078 ± 0.68
5.333AsnGlu: 5.333 ± 0.789
2.196AsnPhe: 2.196 ± 0.62
5.96AsnGly: 5.96 ± 0.773
0.627AsnHis: 0.627 ± 0.214
3.66AsnIle: 3.66 ± 0.585
6.274AsnLys: 6.274 ± 1.174
6.379AsnLeu: 6.379 ± 0.717
1.882AsnMet: 1.882 ± 0.481
3.66AsnAsn: 3.66 ± 0.617
2.196AsnPro: 2.196 ± 0.524
1.987AsnGln: 1.987 ± 0.501
1.882AsnArg: 1.882 ± 0.349
5.124AsnSer: 5.124 ± 0.679
3.974AsnThr: 3.974 ± 0.743
3.242AsnVal: 3.242 ± 0.486
1.359AsnTrp: 1.359 ± 0.383
2.614AsnTyr: 2.614 ± 0.684
0.0AsnXaa: 0.0 ± 0.0
Pro
1.046ProAla: 1.046 ± 0.37
0.209ProCys: 0.209 ± 0.145
1.673ProAsp: 1.673 ± 0.412
2.091ProGlu: 2.091 ± 0.512
1.15ProPhe: 1.15 ± 0.274
0.418ProGly: 0.418 ± 0.169
0.105ProHis: 0.105 ± 0.111
1.987ProIle: 1.987 ± 0.414
1.569ProLys: 1.569 ± 0.421
1.882ProLeu: 1.882 ± 0.381
0.627ProMet: 0.627 ± 0.227
1.778ProAsn: 1.778 ± 0.694
0.523ProPro: 0.523 ± 0.23
0.837ProGln: 0.837 ± 0.271
0.523ProArg: 0.523 ± 0.196
1.046ProSer: 1.046 ± 0.298
2.405ProThr: 2.405 ± 0.435
1.15ProVal: 1.15 ± 0.381
0.105ProTrp: 0.105 ± 0.128
1.15ProTyr: 1.15 ± 0.43
0.0ProXaa: 0.0 ± 0.0
Gln
3.033GlnAla: 3.033 ± 0.68
0.209GlnCys: 0.209 ± 0.154
1.673GlnAsp: 1.673 ± 0.494
2.405GlnGlu: 2.405 ± 0.415
0.941GlnPhe: 0.941 ± 0.318
2.928GlnGly: 2.928 ± 0.523
0.209GlnHis: 0.209 ± 0.129
1.673GlnIle: 1.673 ± 0.337
2.51GlnLys: 2.51 ± 0.494
3.974GlnLeu: 3.974 ± 0.729
1.046GlnMet: 1.046 ± 0.283
2.301GlnAsn: 2.301 ± 0.434
1.15GlnPro: 1.15 ± 0.329
1.882GlnGln: 1.882 ± 0.429
1.778GlnArg: 1.778 ± 0.512
2.091GlnSer: 2.091 ± 0.422
2.51GlnThr: 2.51 ± 0.518
2.719GlnVal: 2.719 ± 0.59
0.732GlnTrp: 0.732 ± 0.229
1.359GlnTyr: 1.359 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
2.301ArgAla: 2.301 ± 0.618
0.418ArgCys: 0.418 ± 0.217
1.569ArgAsp: 1.569 ± 0.407
1.987ArgGlu: 1.987 ± 0.425
0.627ArgPhe: 0.627 ± 0.235
2.51ArgGly: 2.51 ± 0.535
0.941ArgHis: 0.941 ± 0.263
2.091ArgIle: 2.091 ± 0.587
4.078ArgLys: 4.078 ± 0.823
3.137ArgLeu: 3.137 ± 0.484
0.523ArgMet: 0.523 ± 0.262
2.301ArgAsn: 2.301 ± 0.592
0.627ArgPro: 0.627 ± 0.19
1.255ArgGln: 1.255 ± 0.308
1.673ArgArg: 1.673 ± 0.458
1.778ArgSer: 1.778 ± 0.412
1.569ArgThr: 1.569 ± 0.3
1.882ArgVal: 1.882 ± 0.411
0.523ArgTrp: 0.523 ± 0.177
1.569ArgTyr: 1.569 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
4.287SerAla: 4.287 ± 1.523
0.732SerCys: 0.732 ± 0.297
4.287SerAsp: 4.287 ± 0.567
4.078SerGlu: 4.078 ± 0.607
3.033SerPhe: 3.033 ± 0.719
6.483SerGly: 6.483 ± 1.468
0.627SerHis: 0.627 ± 0.252
5.019SerIle: 5.019 ± 0.822
4.287SerLys: 4.287 ± 0.705
5.438SerLeu: 5.438 ± 0.789
1.882SerMet: 1.882 ± 0.456
3.66SerAsn: 3.66 ± 0.677
1.15SerPro: 1.15 ± 0.353
1.569SerGln: 1.569 ± 0.359
2.614SerArg: 2.614 ± 0.351
4.392SerSer: 4.392 ± 0.769
3.242SerThr: 3.242 ± 0.812
3.555SerVal: 3.555 ± 0.611
0.523SerTrp: 0.523 ± 0.185
2.196SerTyr: 2.196 ± 0.514
0.0SerXaa: 0.0 ± 0.0
Thr
4.81ThrAla: 4.81 ± 0.581
0.314ThrCys: 0.314 ± 0.198
3.765ThrAsp: 3.765 ± 0.731
5.647ThrGlu: 5.647 ± 0.639
2.614ThrPhe: 2.614 ± 0.524
4.601ThrGly: 4.601 ± 0.606
0.105ThrHis: 0.105 ± 0.104
3.765ThrIle: 3.765 ± 0.56
4.915ThrLys: 4.915 ± 0.715
5.751ThrLeu: 5.751 ± 0.643
1.255ThrMet: 1.255 ± 0.379
4.601ThrAsn: 4.601 ± 0.736
1.673ThrPro: 1.673 ± 0.407
2.405ThrGln: 2.405 ± 0.496
2.091ThrArg: 2.091 ± 0.452
4.078ThrSer: 4.078 ± 0.648
4.183ThrThr: 4.183 ± 0.569
4.706ThrVal: 4.706 ± 1.026
1.15ThrTrp: 1.15 ± 0.351
1.778ThrTyr: 1.778 ± 0.457
0.0ThrXaa: 0.0 ± 0.0
Val
3.137ValAla: 3.137 ± 0.491
0.837ValCys: 0.837 ± 0.409
4.287ValAsp: 4.287 ± 0.674
4.601ValGlu: 4.601 ± 0.66
3.033ValPhe: 3.033 ± 0.569
3.66ValGly: 3.66 ± 0.698
0.837ValHis: 0.837 ± 0.25
4.601ValIle: 4.601 ± 0.552
6.797ValLys: 6.797 ± 0.65
3.66ValLeu: 3.66 ± 0.552
1.359ValMet: 1.359 ± 0.394
3.66ValAsn: 3.66 ± 0.68
1.569ValPro: 1.569 ± 0.357
1.778ValGln: 1.778 ± 0.363
3.137ValArg: 3.137 ± 0.623
5.019ValSer: 5.019 ± 1.305
5.019ValThr: 5.019 ± 0.895
3.765ValVal: 3.765 ± 0.786
0.418ValTrp: 0.418 ± 0.168
3.033ValTyr: 3.033 ± 0.503
0.0ValXaa: 0.0 ± 0.0
Trp
0.837TrpAla: 0.837 ± 0.278
0.418TrpCys: 0.418 ± 0.21
1.046TrpAsp: 1.046 ± 0.363
0.732TrpGlu: 0.732 ± 0.278
0.941TrpPhe: 0.941 ± 0.318
1.046TrpGly: 1.046 ± 0.322
0.105TrpHis: 0.105 ± 0.116
0.941TrpIle: 0.941 ± 0.359
0.418TrpLys: 0.418 ± 0.194
0.941TrpLeu: 0.941 ± 0.315
0.418TrpMet: 0.418 ± 0.2
1.882TrpAsn: 1.882 ± 0.462
0.105TrpPro: 0.105 ± 0.111
0.732TrpGln: 0.732 ± 0.273
0.418TrpArg: 0.418 ± 0.223
1.046TrpSer: 1.046 ± 0.274
0.627TrpThr: 0.627 ± 0.252
0.627TrpVal: 0.627 ± 0.241
0.314TrpTrp: 0.314 ± 0.17
0.941TrpTyr: 0.941 ± 0.301
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.359TyrAla: 1.359 ± 0.368
0.523TyrCys: 0.523 ± 0.256
2.719TyrAsp: 2.719 ± 0.85
3.869TyrGlu: 3.869 ± 0.736
2.51TyrPhe: 2.51 ± 0.457
2.614TyrGly: 2.614 ± 0.51
0.941TyrHis: 0.941 ± 0.292
3.555TyrIle: 3.555 ± 0.703
2.614TyrLys: 2.614 ± 0.565
3.765TyrLeu: 3.765 ± 0.685
0.732TyrMet: 0.732 ± 0.285
3.869TyrAsn: 3.869 ± 0.597
0.732TyrPro: 0.732 ± 0.288
1.882TyrGln: 1.882 ± 0.47
1.046TyrArg: 1.046 ± 0.325
1.882TyrSer: 1.882 ± 0.44
2.928TyrThr: 2.928 ± 0.618
2.51TyrVal: 2.51 ± 0.544
0.209TyrTrp: 0.209 ± 0.142
2.091TyrTyr: 2.091 ± 0.439
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (9564 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski