Bat Paramyxovirus Eid_hel/GH-M74a/GHA/2009

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Henipavirus; Ghanaian bat henipavirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|I0E090|I0E090_9MONO Uncharacterized protein OS=Bat Paramyxovirus Eid_hel/GH-M74a/GHA/2009 OX=1112631 GN=C PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 5.25KK3 pKa = 10.97EE4 pKa = 4.41SLVDD8 pKa = 3.57NGIKK12 pKa = 9.82IIEE15 pKa = 4.93FIQKK19 pKa = 10.15NKK21 pKa = 11.08DD22 pKa = 3.7EE23 pKa = 4.19IQKK26 pKa = 9.59TYY28 pKa = 10.63GRR30 pKa = 11.84SQIQEE35 pKa = 3.5PRR37 pKa = 11.84TRR39 pKa = 11.84EE40 pKa = 3.36RR41 pKa = 11.84AAAWEE46 pKa = 3.91LFIRR50 pKa = 11.84GEE52 pKa = 4.11NPRR55 pKa = 11.84PEE57 pKa = 4.81GDD59 pKa = 2.96RR60 pKa = 11.84MAGVDD65 pKa = 3.44QQDD68 pKa = 3.46PTEE71 pKa = 4.41RR72 pKa = 11.84RR73 pKa = 11.84DD74 pKa = 3.57DD75 pKa = 4.01DD76 pKa = 3.98GRR78 pKa = 11.84PNTLARR84 pKa = 11.84DD85 pKa = 4.04GEE87 pKa = 4.38IGEE90 pKa = 4.68SGPNYY95 pKa = 9.84ISWSSGGDD103 pKa = 3.84DD104 pKa = 3.33IQLGPMVQDD113 pKa = 5.5FEE115 pKa = 6.01IDD117 pKa = 3.51DD118 pKa = 4.18NGIKK122 pKa = 10.43LPNSRR127 pKa = 11.84GSKK130 pKa = 9.04SAPRR134 pKa = 11.84CRR136 pKa = 11.84SVDD139 pKa = 3.15KK140 pKa = 11.16SRR142 pKa = 11.84ASDD145 pKa = 3.53WEE147 pKa = 3.91RR148 pKa = 11.84SPASNIDD155 pKa = 3.38HH156 pKa = 7.3LSNLSGGGKK165 pKa = 10.04DD166 pKa = 3.06LGNYY170 pKa = 8.22IKK172 pKa = 10.81NVGVMVYY179 pKa = 9.25PPTGAGVDD187 pKa = 3.7RR188 pKa = 11.84NSLTKK193 pKa = 9.84PVSGVRR199 pKa = 11.84QSRR202 pKa = 11.84LSKK205 pKa = 9.65LTWTKK210 pKa = 10.65EE211 pKa = 4.09SPLDD215 pKa = 3.59QPEE218 pKa = 4.5TVPEE222 pKa = 4.25TLNDD226 pKa = 3.41AYY228 pKa = 10.85VIKK231 pKa = 10.31EE232 pKa = 3.92ATSKK236 pKa = 11.27KK237 pKa = 10.17EE238 pKa = 3.72VTPEE242 pKa = 3.84DD243 pKa = 4.74GISILNPNAASFTPRR258 pKa = 11.84STPAPKK264 pKa = 10.19SVEE267 pKa = 3.87KK268 pKa = 10.6EE269 pKa = 3.81PNQRR273 pKa = 11.84QPAGGHH279 pKa = 6.52PEE281 pKa = 4.59DD282 pKa = 4.45KK283 pKa = 10.1GTPNRR288 pKa = 11.84SEE290 pKa = 4.04NPTTKK295 pKa = 9.45STAIKK300 pKa = 9.63NSKK303 pKa = 10.27VITKK307 pKa = 10.63AMVHH311 pKa = 5.97TSPNEE316 pKa = 3.68INQSARR322 pKa = 11.84SMNIEE327 pKa = 4.63FEE329 pKa = 4.49PKK331 pKa = 10.25SNPSTLTLEE340 pKa = 4.45TVQPSNQQTNNTPASHH356 pKa = 7.41PSGKK360 pKa = 6.65TTRR363 pKa = 11.84EE364 pKa = 4.08LPKK367 pKa = 10.47GAQPQSGTSRR377 pKa = 11.84AANIKK382 pKa = 10.76DD383 pKa = 3.41PTTAPNKK390 pKa = 9.8TSIQTKK396 pKa = 7.43PARR399 pKa = 11.84NASGGDD405 pKa = 3.34GTNPQMASPPPPPVVTNPPLKK426 pKa = 10.48KK427 pKa = 9.83RR428 pKa = 11.84VPKK431 pKa = 10.57LSEE434 pKa = 4.21PNTRR438 pKa = 11.84DD439 pKa = 3.65HH440 pKa = 7.1LSHH443 pKa = 6.69PTDD446 pKa = 3.26EE447 pKa = 4.82KK448 pKa = 10.8RR449 pKa = 11.84DD450 pKa = 3.66HH451 pKa = 6.87GGPAVKK457 pKa = 10.3SKK459 pKa = 8.58TQAHH463 pKa = 6.72RR464 pKa = 11.84GLNADD469 pKa = 3.51NTQNCTDD476 pKa = 3.41QPTPSSNKK484 pKa = 8.99AQPPKK489 pKa = 10.05TPKK492 pKa = 10.12PEE494 pKa = 4.0NPQAEE499 pKa = 4.75PKK501 pKa = 9.2PKK503 pKa = 10.2KK504 pKa = 9.77HH505 pKa = 6.5VSFPNLDD512 pKa = 3.43LTIEE516 pKa = 4.34EE517 pKa = 4.43EE518 pKa = 4.21GGTAVDD524 pKa = 3.14IMVNEE529 pKa = 4.17TRR531 pKa = 11.84EE532 pKa = 4.12GVIDD536 pKa = 3.27VHH538 pKa = 7.41LIKK541 pKa = 10.58EE542 pKa = 4.22DD543 pKa = 3.71NKK545 pKa = 10.34ILNMDD550 pKa = 3.6QDD552 pKa = 4.58NPCSNQITSQQEE564 pKa = 3.81TGVTKK569 pKa = 10.61RR570 pKa = 11.84GIGEE574 pKa = 3.94NSSCIGTKK582 pKa = 9.73EE583 pKa = 4.76DD584 pKa = 3.45SRR586 pKa = 11.84QLSGAIQSAQKK597 pKa = 10.52SRR599 pKa = 11.84LSQRR603 pKa = 11.84QEE605 pKa = 3.6NASAEE610 pKa = 4.31SARR613 pKa = 11.84PGVTSVPSDD622 pKa = 3.48DD623 pKa = 4.41PEE625 pKa = 4.8SEE627 pKa = 4.06EE628 pKa = 4.05VKK630 pKa = 10.05TYY632 pKa = 10.26RR633 pKa = 11.84ASDD636 pKa = 4.16AIIEE640 pKa = 4.01QLNNDD645 pKa = 3.92EE646 pKa = 4.4AQDD649 pKa = 3.96YY650 pKa = 11.3YY651 pKa = 11.95SFLDD655 pKa = 3.79LMEE658 pKa = 4.64NTDD661 pKa = 5.61DD662 pKa = 5.85DD663 pKa = 5.44DD664 pKa = 5.81LICEE668 pKa = 4.11EE669 pKa = 4.82TKK671 pKa = 10.71FSLLNTARR679 pKa = 11.84LISLNSRR686 pKa = 11.84IEE688 pKa = 4.24KK689 pKa = 10.06IEE691 pKa = 3.92EE692 pKa = 3.99QIKK695 pKa = 10.21KK696 pKa = 9.49IPAMEE701 pKa = 4.6KK702 pKa = 10.18KK703 pKa = 10.69LSDD706 pKa = 3.69IEE708 pKa = 4.32KK709 pKa = 10.79LLLKK713 pKa = 10.06TNTALSTIEE722 pKa = 3.82GHH724 pKa = 5.73ITSMMIMVPGKK735 pKa = 8.11TVNEE739 pKa = 4.11GEE741 pKa = 4.16IQINEE746 pKa = 3.91QLKK749 pKa = 9.89PVIGRR754 pKa = 11.84KK755 pKa = 9.93DD756 pKa = 3.35GVYY759 pKa = 10.98DD760 pKa = 3.97KK761 pKa = 10.6MILPRR766 pKa = 11.84VDD768 pKa = 3.45KK769 pKa = 10.75KK770 pKa = 10.97ISLVTDD776 pKa = 4.14RR777 pKa = 11.84TPLCDD782 pKa = 4.89DD783 pKa = 4.45FKK785 pKa = 11.22EE786 pKa = 4.39DD787 pKa = 4.46PYY789 pKa = 11.85VLNEE793 pKa = 4.28KK794 pKa = 10.59LILDD798 pKa = 5.27PINPSVTNASAFIPSYY814 pKa = 11.43DD815 pKa = 3.9NISPAVLRR823 pKa = 11.84SLIRR827 pKa = 11.84SNVEE831 pKa = 3.52DD832 pKa = 3.74RR833 pKa = 11.84EE834 pKa = 4.44TRR836 pKa = 11.84VEE838 pKa = 4.69LIEE841 pKa = 5.06LVNQARR847 pKa = 11.84NDD849 pKa = 3.71SEE851 pKa = 4.76LNEE854 pKa = 3.95ILALVNDD861 pKa = 5.38IIDD864 pKa = 4.05SNQSGVV870 pKa = 3.08

Molecular weight:
95.52 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|I0E092|I0E092_9MONO Fusion glycoprotein F0 OS=Bat Paramyxovirus Eid_hel/GH-M74a/GHA/2009 OX=1112631 GN=F PE=3 SV=1
MM1 pKa = 7.25EE2 pKa = 6.66AITNFMPYY10 pKa = 7.5TWEE13 pKa = 4.35FGGSLNKK20 pKa = 9.69VEE22 pKa = 5.3PEE24 pKa = 3.66LDD26 pKa = 3.78EE27 pKa = 4.7NGSMIPKK34 pKa = 9.6VRR36 pKa = 11.84VLNPGYY42 pKa = 10.43NDD44 pKa = 3.74RR45 pKa = 11.84KK46 pKa = 10.09SSGYY50 pKa = 10.21QYY52 pKa = 10.16LICYY56 pKa = 8.97GFVEE60 pKa = 5.43DD61 pKa = 5.92IRR63 pKa = 11.84SEE65 pKa = 4.41SNGDD69 pKa = 3.24KK70 pKa = 10.71KK71 pKa = 10.37PTIRR75 pKa = 11.84TTASFPLGVGRR86 pKa = 11.84SDD88 pKa = 3.9ASPEE92 pKa = 3.83NLLDD96 pKa = 4.02EE97 pKa = 4.45VCKK100 pKa = 10.91LSITVRR106 pKa = 11.84RR107 pKa = 11.84TSGAEE112 pKa = 3.56EE113 pKa = 4.26KK114 pKa = 10.25IVYY117 pKa = 9.16GVSGGILYY125 pKa = 10.24LDD127 pKa = 3.59PWKK130 pKa = 10.75KK131 pKa = 9.98VLKK134 pKa = 10.51NGAIFTATKK143 pKa = 8.33VCRR146 pKa = 11.84NIEE149 pKa = 4.11CVLVDD154 pKa = 2.93KK155 pKa = 10.7HH156 pKa = 5.78QSLRR160 pKa = 11.84IFFLSITKK168 pKa = 8.83LTDD171 pKa = 2.71RR172 pKa = 11.84GYY174 pKa = 8.51YY175 pKa = 8.3TIPRR179 pKa = 11.84RR180 pKa = 11.84MLEE183 pKa = 3.61FRR185 pKa = 11.84YY186 pKa = 10.9NNAIALNLLVTLAISTDD203 pKa = 3.48TTKK206 pKa = 11.0SGVRR210 pKa = 11.84GIKK213 pKa = 10.43NEE215 pKa = 4.22DD216 pKa = 3.4GFTLVTFMTHH226 pKa = 5.24MGNFQRR232 pKa = 11.84RR233 pKa = 11.84AGKK236 pKa = 8.44TYY238 pKa = 10.29SLEE241 pKa = 4.02YY242 pKa = 10.3CSRR245 pKa = 11.84KK246 pKa = 8.19VDD248 pKa = 3.32KK249 pKa = 10.64MKK251 pKa = 10.56MKK253 pKa = 10.57FSLGAIGGLSFHH265 pKa = 6.03VRR267 pKa = 11.84IDD269 pKa = 3.57GVISKK274 pKa = 10.54RR275 pKa = 11.84LFAEE279 pKa = 3.66MGFRR283 pKa = 11.84RR284 pKa = 11.84NICYY288 pKa = 10.22CLIDD292 pKa = 3.7INPWINKK299 pKa = 6.94LTWSNTCEE307 pKa = 3.95IRR309 pKa = 11.84NVAAVLQPSVPRR321 pKa = 11.84DD322 pKa = 3.04FMIYY326 pKa = 10.67DD327 pKa = 4.21DD328 pKa = 4.75VFIDD332 pKa = 3.62NTGKK336 pKa = 10.27ILPSKK341 pKa = 10.14HH342 pKa = 5.56AA343 pKa = 4.26

Molecular weight:
38.81 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

5433

162

2250

776.1

87.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.822 ± 0.521

1.546 ± 0.305

5.393 ± 0.315

6.203 ± 0.442

3.258 ± 0.586

5.154 ± 0.446

1.859 ± 0.358

8.448 ± 0.674

6.35 ± 0.438

9.074 ± 0.648

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.217

6.037 ± 0.438

4.859 ± 0.762

3.792 ± 0.298

5.503 ± 0.4

8.32 ± 0.308

6.534 ± 0.563

5.54 ± 0.142

0.92 ± 0.152

3.828 ± 0.583

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski