Amino acid dipepetide frequency for Streptomyces phage Rainydai

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.025AlaAla: 10.025 ± 1.209
0.443AlaCys: 0.443 ± 0.178
4.929AlaAsp: 4.929 ± 0.647
6.314AlaGlu: 6.314 ± 0.576
2.492AlaPhe: 2.492 ± 0.399
7.976AlaGly: 7.976 ± 1.356
1.218AlaHis: 1.218 ± 0.275
4.819AlaIle: 4.819 ± 0.596
7.145AlaLys: 7.145 ± 0.766
7.588AlaLeu: 7.588 ± 1.286
3.046AlaMet: 3.046 ± 0.384
2.935AlaAsn: 2.935 ± 0.52
3.046AlaPro: 3.046 ± 0.425
3.046AlaGln: 3.046 ± 0.558
4.819AlaArg: 4.819 ± 0.66
4.763AlaSer: 4.763 ± 0.599
5.151AlaThr: 5.151 ± 0.553
6.646AlaVal: 6.646 ± 0.554
1.44AlaTrp: 1.44 ± 0.317
2.991AlaTyr: 2.991 ± 0.39
0.0AlaXaa: 0.0 ± 0.0
Cys
0.388CysAla: 0.388 ± 0.174
0.222CysCys: 0.222 ± 0.101
0.332CysAsp: 0.332 ± 0.146
0.166CysGlu: 0.166 ± 0.082
0.111CysPhe: 0.111 ± 0.072
0.443CysGly: 0.443 ± 0.216
0.111CysHis: 0.111 ± 0.071
0.498CysIle: 0.498 ± 0.168
0.222CysLys: 0.222 ± 0.127
0.665CysLeu: 0.665 ± 0.192
0.055CysMet: 0.055 ± 0.051
0.498CysAsn: 0.498 ± 0.213
0.222CysPro: 0.222 ± 0.115
0.111CysGln: 0.111 ± 0.078
0.055CysArg: 0.055 ± 0.048
0.831CysSer: 0.831 ± 0.226
0.388CysThr: 0.388 ± 0.174
0.498CysVal: 0.498 ± 0.165
0.166CysTrp: 0.166 ± 0.082
0.166CysTyr: 0.166 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
5.539AspAla: 5.539 ± 0.713
0.554AspCys: 0.554 ± 0.18
3.323AspAsp: 3.323 ± 0.462
5.816AspGlu: 5.816 ± 0.807
2.215AspPhe: 2.215 ± 0.401
5.816AspGly: 5.816 ± 0.774
0.886AspHis: 0.886 ± 0.255
2.825AspIle: 2.825 ± 0.488
2.88AspLys: 2.88 ± 0.344
4.486AspLeu: 4.486 ± 0.503
1.772AspMet: 1.772 ± 0.266
2.105AspAsn: 2.105 ± 0.384
3.932AspPro: 3.932 ± 0.537
2.215AspGln: 2.215 ± 0.32
3.046AspArg: 3.046 ± 0.448
3.877AspSer: 3.877 ± 0.486
3.766AspThr: 3.766 ± 0.401
4.32AspVal: 4.32 ± 0.481
1.052AspTrp: 1.052 ± 0.236
1.772AspTyr: 1.772 ± 0.368
0.0AspXaa: 0.0 ± 0.0
Glu
4.763GluAla: 4.763 ± 0.533
0.388GluCys: 0.388 ± 0.17
4.652GluAsp: 4.652 ± 0.681
6.536GluGlu: 6.536 ± 1.11
3.822GluPhe: 3.822 ± 0.548
4.265GluGly: 4.265 ± 0.552
1.495GluHis: 1.495 ± 0.337
4.099GluIle: 4.099 ± 0.557
4.985GluLys: 4.985 ± 0.558
5.871GluLeu: 5.871 ± 0.526
2.437GluMet: 2.437 ± 0.37
2.437GluAsn: 2.437 ± 0.429
2.659GluPro: 2.659 ± 0.472
2.215GluGln: 2.215 ± 0.346
4.542GluArg: 4.542 ± 0.59
3.379GluSer: 3.379 ± 0.471
4.763GluThr: 4.763 ± 0.599
4.652GluVal: 4.652 ± 0.58
1.274GluTrp: 1.274 ± 0.253
1.939GluTyr: 1.939 ± 0.285
0.0GluXaa: 0.0 ± 0.0
Phe
2.88PheAla: 2.88 ± 0.334
0.222PheCys: 0.222 ± 0.135
3.157PheAsp: 3.157 ± 0.414
2.659PheGlu: 2.659 ± 0.31
1.495PhePhe: 1.495 ± 0.293
2.991PheGly: 2.991 ± 0.547
0.609PheHis: 0.609 ± 0.176
1.994PheIle: 1.994 ± 0.328
2.16PheLys: 2.16 ± 0.333
2.825PheLeu: 2.825 ± 0.426
1.052PheMet: 1.052 ± 0.218
1.662PheAsn: 1.662 ± 0.408
1.108PhePro: 1.108 ± 0.173
1.772PheGln: 1.772 ± 0.332
1.883PheArg: 1.883 ± 0.308
2.215PheSer: 2.215 ± 0.422
2.548PheThr: 2.548 ± 0.46
2.548PheVal: 2.548 ± 0.372
0.665PheTrp: 0.665 ± 0.221
1.218PheTyr: 1.218 ± 0.342
0.0PheXaa: 0.0 ± 0.0
Gly
7.865GlyAla: 7.865 ± 0.845
0.388GlyCys: 0.388 ± 0.174
5.372GlyAsp: 5.372 ± 0.469
4.597GlyGlu: 4.597 ± 0.49
3.489GlyPhe: 3.489 ± 0.68
4.486GlyGly: 4.486 ± 0.688
1.163GlyHis: 1.163 ± 0.255
4.985GlyIle: 4.985 ± 0.875
4.099GlyLys: 4.099 ± 0.486
6.702GlyLeu: 6.702 ± 1.463
1.828GlyMet: 1.828 ± 0.277
2.16GlyAsn: 2.16 ± 0.316
2.935GlyPro: 2.935 ± 0.413
1.606GlyGln: 1.606 ± 0.317
3.212GlyArg: 3.212 ± 0.358
4.376GlySer: 4.376 ± 0.505
5.76GlyThr: 5.76 ± 0.571
6.591GlyVal: 6.591 ± 0.634
1.606GlyTrp: 1.606 ± 0.378
3.489GlyTyr: 3.489 ± 0.602
0.0GlyXaa: 0.0 ± 0.0
His
1.163HisAla: 1.163 ± 0.311
0.332HisCys: 0.332 ± 0.144
0.942HisAsp: 0.942 ± 0.25
0.831HisGlu: 0.831 ± 0.219
0.665HisPhe: 0.665 ± 0.205
1.717HisGly: 1.717 ± 0.39
0.609HisHis: 0.609 ± 0.178
0.942HisIle: 0.942 ± 0.237
0.775HisLys: 0.775 ± 0.199
1.385HisLeu: 1.385 ± 0.298
0.554HisMet: 0.554 ± 0.149
0.388HisAsn: 0.388 ± 0.154
0.831HisPro: 0.831 ± 0.213
0.554HisGln: 0.554 ± 0.203
1.108HisArg: 1.108 ± 0.291
1.44HisSer: 1.44 ± 0.284
0.72HisThr: 0.72 ± 0.203
1.329HisVal: 1.329 ± 0.314
0.166HisTrp: 0.166 ± 0.088
0.886HisTyr: 0.886 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
5.539IleAla: 5.539 ± 0.638
0.222IleCys: 0.222 ± 0.142
3.489IleAsp: 3.489 ± 0.37
4.209IleGlu: 4.209 ± 0.531
2.659IlePhe: 2.659 ± 0.408
4.874IleGly: 4.874 ± 0.851
1.052IleHis: 1.052 ± 0.28
1.883IleIle: 1.883 ± 0.405
3.323IleLys: 3.323 ± 0.436
4.652IleLeu: 4.652 ± 0.536
1.163IleMet: 1.163 ± 0.232
1.828IleAsn: 1.828 ± 0.296
2.382IlePro: 2.382 ± 0.324
1.772IleGln: 1.772 ± 0.286
2.603IleArg: 2.603 ± 0.341
2.991IleSer: 2.991 ± 0.498
3.988IleThr: 3.988 ± 0.558
3.711IleVal: 3.711 ± 0.498
0.775IleTrp: 0.775 ± 0.209
1.385IleTyr: 1.385 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
6.425LysAla: 6.425 ± 0.876
0.332LysCys: 0.332 ± 0.138
2.659LysAsp: 2.659 ± 0.461
3.932LysGlu: 3.932 ± 0.535
1.662LysPhe: 1.662 ± 0.324
4.099LysGly: 4.099 ± 0.668
0.886LysHis: 0.886 ± 0.261
3.711LysIle: 3.711 ± 0.464
5.096LysLys: 5.096 ± 0.639
5.649LysLeu: 5.649 ± 0.73
1.939LysMet: 1.939 ± 0.299
3.212LysAsn: 3.212 ± 0.5
2.991LysPro: 2.991 ± 0.467
1.883LysGln: 1.883 ± 0.315
3.157LysArg: 3.157 ± 0.508
4.099LysSer: 4.099 ± 0.513
4.209LysThr: 4.209 ± 0.657
4.985LysVal: 4.985 ± 0.548
0.886LysTrp: 0.886 ± 0.248
2.271LysTyr: 2.271 ± 0.351
0.0LysXaa: 0.0 ± 0.0
Leu
7.976LeuAla: 7.976 ± 1.192
0.277LeuCys: 0.277 ± 0.124
4.929LeuAsp: 4.929 ± 0.688
5.871LeuGlu: 5.871 ± 0.7
2.935LeuPhe: 2.935 ± 0.434
6.757LeuGly: 6.757 ± 1.286
1.052LeuHis: 1.052 ± 0.286
4.209LeuIle: 4.209 ± 0.495
5.539LeuLys: 5.539 ± 0.95
5.317LeuLeu: 5.317 ± 0.893
2.382LeuMet: 2.382 ± 0.347
3.932LeuAsn: 3.932 ± 0.449
2.769LeuPro: 2.769 ± 0.349
2.714LeuGln: 2.714 ± 0.429
4.099LeuArg: 4.099 ± 0.522
5.317LeuSer: 5.317 ± 0.713
5.76LeuThr: 5.76 ± 0.688
4.708LeuVal: 4.708 ± 0.508
0.886LeuTrp: 0.886 ± 0.22
2.437LeuTyr: 2.437 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
2.659MetAla: 2.659 ± 0.369
0.222MetCys: 0.222 ± 0.117
1.551MetAsp: 1.551 ± 0.31
1.939MetGlu: 1.939 ± 0.413
0.665MetPhe: 0.665 ± 0.216
1.551MetGly: 1.551 ± 0.254
0.609MetHis: 0.609 ± 0.181
1.551MetIle: 1.551 ± 0.308
1.828MetLys: 1.828 ± 0.356
2.049MetLeu: 2.049 ± 0.458
0.388MetMet: 0.388 ± 0.122
1.163MetAsn: 1.163 ± 0.275
1.274MetPro: 1.274 ± 0.228
0.72MetGln: 0.72 ± 0.212
1.052MetArg: 1.052 ± 0.227
2.049MetSer: 2.049 ± 0.273
1.939MetThr: 1.939 ± 0.3
1.495MetVal: 1.495 ± 0.255
0.222MetTrp: 0.222 ± 0.12
0.886MetTyr: 0.886 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
4.154AsnAla: 4.154 ± 0.415
0.222AsnCys: 0.222 ± 0.145
2.049AsnAsp: 2.049 ± 0.32
2.659AsnGlu: 2.659 ± 0.401
1.772AsnPhe: 1.772 ± 0.374
3.545AsnGly: 3.545 ± 0.525
0.72AsnHis: 0.72 ± 0.177
1.606AsnIle: 1.606 ± 0.3
2.714AsnLys: 2.714 ± 0.407
2.935AsnLeu: 2.935 ± 0.42
0.554AsnMet: 0.554 ± 0.188
1.662AsnAsn: 1.662 ± 0.279
2.382AsnPro: 2.382 ± 0.391
1.495AsnGln: 1.495 ± 0.28
2.326AsnArg: 2.326 ± 0.371
2.215AsnSer: 2.215 ± 0.412
1.939AsnThr: 1.939 ± 0.32
2.603AsnVal: 2.603 ± 0.4
0.554AsnTrp: 0.554 ± 0.199
1.329AsnTyr: 1.329 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
3.434ProAla: 3.434 ± 0.594
0.498ProCys: 0.498 ± 0.173
3.434ProAsp: 3.434 ± 0.504
3.766ProGlu: 3.766 ± 0.504
1.329ProPhe: 1.329 ± 0.264
2.714ProGly: 2.714 ± 0.339
0.831ProHis: 0.831 ± 0.263
1.551ProIle: 1.551 ± 0.242
2.88ProLys: 2.88 ± 0.352
2.769ProLeu: 2.769 ± 0.389
1.052ProMet: 1.052 ± 0.266
2.215ProAsn: 2.215 ± 0.346
1.717ProPro: 1.717 ± 0.371
1.108ProGln: 1.108 ± 0.232
2.382ProArg: 2.382 ± 0.44
2.659ProSer: 2.659 ± 0.556
2.659ProThr: 2.659 ± 0.492
3.6ProVal: 3.6 ± 0.486
0.609ProTrp: 0.609 ± 0.247
1.163ProTyr: 1.163 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
3.046GlnAla: 3.046 ± 0.43
0.166GlnCys: 0.166 ± 0.093
1.883GlnAsp: 1.883 ± 0.335
1.329GlnGlu: 1.329 ± 0.261
1.274GlnPhe: 1.274 ± 0.257
2.382GlnGly: 2.382 ± 0.433
0.886GlnHis: 0.886 ± 0.203
2.16GlnIle: 2.16 ± 0.282
2.049GlnLys: 2.049 ± 0.361
2.492GlnLeu: 2.492 ± 0.491
0.886GlnMet: 0.886 ± 0.198
1.052GlnAsn: 1.052 ± 0.308
1.052GlnPro: 1.052 ± 0.246
1.828GlnGln: 1.828 ± 0.369
1.717GlnArg: 1.717 ± 0.281
1.883GlnSer: 1.883 ± 0.293
2.105GlnThr: 2.105 ± 0.401
2.215GlnVal: 2.215 ± 0.311
0.332GlnTrp: 0.332 ± 0.124
0.942GlnTyr: 0.942 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
3.212ArgAla: 3.212 ± 0.437
0.277ArgCys: 0.277 ± 0.121
3.545ArgAsp: 3.545 ± 0.423
4.265ArgGlu: 4.265 ± 0.49
1.717ArgPhe: 1.717 ± 0.28
2.548ArgGly: 2.548 ± 0.448
0.831ArgHis: 0.831 ± 0.259
2.935ArgIle: 2.935 ± 0.505
3.766ArgLys: 3.766 ± 0.497
4.929ArgLeu: 4.929 ± 0.573
2.049ArgMet: 2.049 ± 0.317
2.326ArgAsn: 2.326 ± 0.329
2.105ArgPro: 2.105 ± 0.446
1.717ArgGln: 1.717 ± 0.315
3.822ArgArg: 3.822 ± 0.628
3.379ArgSer: 3.379 ± 0.524
3.379ArgThr: 3.379 ± 0.58
3.655ArgVal: 3.655 ± 0.535
0.609ArgTrp: 0.609 ± 0.234
1.717ArgTyr: 1.717 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
5.262SerAla: 5.262 ± 0.764
0.111SerCys: 0.111 ± 0.063
3.988SerAsp: 3.988 ± 0.471
3.489SerGlu: 3.489 ± 0.44
2.049SerPhe: 2.049 ± 0.351
6.259SerGly: 6.259 ± 0.615
0.886SerHis: 0.886 ± 0.244
3.157SerIle: 3.157 ± 0.397
3.212SerLys: 3.212 ± 0.514
5.428SerLeu: 5.428 ± 0.568
1.218SerMet: 1.218 ± 0.243
1.994SerAsn: 1.994 ± 0.324
2.991SerPro: 2.991 ± 0.444
1.606SerGln: 1.606 ± 0.346
3.323SerArg: 3.323 ± 0.464
4.32SerSer: 4.32 ± 0.737
3.932SerThr: 3.932 ± 0.525
4.099SerVal: 4.099 ± 0.475
1.218SerTrp: 1.218 ± 0.239
1.828SerTyr: 1.828 ± 0.282
0.0SerXaa: 0.0 ± 0.0
Thr
5.649ThrAla: 5.649 ± 0.651
0.332ThrCys: 0.332 ± 0.185
3.711ThrAsp: 3.711 ± 0.507
4.542ThrGlu: 4.542 ± 0.559
2.88ThrPhe: 2.88 ± 0.475
5.705ThrGly: 5.705 ± 0.795
1.218ThrHis: 1.218 ± 0.265
4.486ThrIle: 4.486 ± 0.558
3.988ThrLys: 3.988 ± 0.521
5.262ThrLeu: 5.262 ± 0.547
0.831ThrMet: 0.831 ± 0.182
2.105ThrAsn: 2.105 ± 0.335
3.489ThrPro: 3.489 ± 0.528
2.271ThrGln: 2.271 ± 0.458
3.157ThrArg: 3.157 ± 0.449
2.825ThrSer: 2.825 ± 0.522
4.819ThrThr: 4.819 ± 0.866
5.816ThrVal: 5.816 ± 0.772
0.942ThrTrp: 0.942 ± 0.236
1.994ThrTyr: 1.994 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
6.037ValAla: 6.037 ± 0.555
0.554ValCys: 0.554 ± 0.215
4.542ValAsp: 4.542 ± 0.421
5.372ValGlu: 5.372 ± 0.652
2.326ValPhe: 2.326 ± 0.377
5.206ValGly: 5.206 ± 0.529
1.218ValHis: 1.218 ± 0.311
4.819ValIle: 4.819 ± 0.461
4.597ValLys: 4.597 ± 0.516
5.594ValLeu: 5.594 ± 0.551
1.606ValMet: 1.606 ± 0.288
2.769ValAsn: 2.769 ± 0.314
2.548ValPro: 2.548 ± 0.404
1.772ValGln: 1.772 ± 0.421
4.099ValArg: 4.099 ± 0.589
4.32ValSer: 4.32 ± 0.45
5.816ValThr: 5.816 ± 0.668
4.763ValVal: 4.763 ± 0.538
1.052ValTrp: 1.052 ± 0.29
2.991ValTyr: 2.991 ± 0.493
0.0ValXaa: 0.0 ± 0.0
Trp
1.551TrpAla: 1.551 ± 0.334
0.222TrpCys: 0.222 ± 0.131
1.108TrpAsp: 1.108 ± 0.328
1.163TrpGlu: 1.163 ± 0.242
0.665TrpPhe: 0.665 ± 0.217
1.108TrpGly: 1.108 ± 0.269
0.277TrpHis: 0.277 ± 0.122
0.775TrpIle: 0.775 ± 0.239
0.665TrpLys: 0.665 ± 0.261
1.108TrpLeu: 1.108 ± 0.266
0.277TrpMet: 0.277 ± 0.133
1.385TrpAsn: 1.385 ± 0.372
0.277TrpPro: 0.277 ± 0.117
0.222TrpGln: 0.222 ± 0.095
0.72TrpArg: 0.72 ± 0.222
1.218TrpSer: 1.218 ± 0.395
0.886TrpThr: 0.886 ± 0.336
1.108TrpVal: 1.108 ± 0.28
0.388TrpTrp: 0.388 ± 0.172
0.443TrpTyr: 0.443 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.268TyrAla: 3.268 ± 0.396
0.166TyrCys: 0.166 ± 0.089
2.603TyrAsp: 2.603 ± 0.39
1.772TyrGlu: 1.772 ± 0.432
1.44TyrPhe: 1.44 ± 0.34
2.271TyrGly: 2.271 ± 0.357
0.775TyrHis: 0.775 ± 0.252
1.495TyrIle: 1.495 ± 0.31
2.16TyrLys: 2.16 ± 0.446
2.16TyrLeu: 2.16 ± 0.388
0.665TyrMet: 0.665 ± 0.224
1.606TyrAsn: 1.606 ± 0.255
1.662TyrPro: 1.662 ± 0.273
1.052TyrGln: 1.052 ± 0.183
1.662TyrArg: 1.662 ± 0.257
2.215TyrSer: 2.215 ± 0.356
1.495TyrThr: 1.495 ± 0.343
2.659TyrVal: 2.659 ± 0.386
0.72TyrTrp: 0.72 ± 0.25
1.606TyrTyr: 1.606 ± 0.399
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (18056 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski