Amino acid dipepetide frequency for Actinomyces howellii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.366AlaAla: 20.366 ± 0.244	1.224AlaCys: 1.224 ± 0.042	7.599AlaAsp: 7.599 ± 0.108	8.197AlaGlu: 8.197 ± 0.133	2.862AlaPhe: 2.862 ± 0.068	13.534AlaGly: 13.534 ± 0.15	2.49AlaHis: 2.49 ± 0.062	3.504AlaIle: 3.504 ± 0.074	1.687AlaLys: 1.687 ± 0.055	14.685AlaLeu: 14.685 ± 0.154	2.675AlaMet: 2.675 ± 0.055	1.811AlaAsn: 1.811 ± 0.045	7.728AlaPro: 7.728 ± 0.12	5.008AlaGln: 5.008 ± 0.083	11.104AlaArg: 11.104 ± 0.16	7.856AlaSer: 7.856 ± 0.129	7.736AlaThr: 7.736 ± 0.118	12.688AlaVal: 12.688 ± 0.13	2.149AlaTrp: 2.149 ± 0.055	2.326AlaTyr: 2.326 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	1.064CysAla: 1.064 ± 0.037	0.09CysCys: 0.09 ± 0.01	0.411CysAsp: 0.411 ± 0.023	0.432CysGlu: 0.432 ± 0.023	0.192CysPhe: 0.192 ± 0.017	0.778CysGly: 0.778 ± 0.032	0.174CysHis: 0.174 ± 0.017	0.186CysIle: 0.186 ± 0.013	0.066CysLys: 0.066 ± 0.007	0.862CysLeu: 0.862 ± 0.035	0.115CysMet: 0.115 ± 0.011	0.124CysAsn: 0.124 ± 0.012	0.521CysPro: 0.521 ± 0.031	0.243CysGln: 0.243 ± 0.017	0.555CysArg: 0.555 ± 0.028	0.488CysSer: 0.488 ± 0.027	0.529CysThr: 0.529 ± 0.028	0.642CysVal: 0.642 ± 0.029	0.126CysTrp: 0.126 ± 0.011	0.133CysTyr: 0.133 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.284AspAla: 7.284 ± 0.099	0.393AspCys: 0.393 ± 0.019	3.381AspAsp: 3.381 ± 0.078	3.917AspGlu: 3.917 ± 0.066	1.41AspPhe: 1.41 ± 0.041	5.769AspGly: 5.769 ± 0.096	1.332AspHis: 1.332 ± 0.035	1.744AspIle: 1.744 ± 0.052	1.017AspLys: 1.017 ± 0.042	6.62AspLeu: 6.62 ± 0.101	0.947AspMet: 0.947 ± 0.035	0.94AspAsn: 0.94 ± 0.042	5.092AspPro: 5.092 ± 0.09	1.618AspGln: 1.618 ± 0.044	4.323AspArg: 4.323 ± 0.086	3.056AspSer: 3.056 ± 0.062	2.909AspThr: 2.909 ± 0.062	4.772AspVal: 4.772 ± 0.075	0.817AspTrp: 0.817 ± 0.037	1.233AspTyr: 1.233 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	8.821GluAla: 8.821 ± 0.154	0.335GluCys: 0.335 ± 0.019	3.701GluAsp: 3.701 ± 0.07	3.822GluGlu: 3.822 ± 0.081	1.02GluPhe: 1.02 ± 0.038	4.747GluGly: 4.747 ± 0.078	1.567GluHis: 1.567 ± 0.046	2.25GluIle: 2.25 ± 0.053	0.765GluLys: 0.765 ± 0.032	6.35GluLeu: 6.35 ± 0.097	1.028GluMet: 1.028 ± 0.036	0.778GluAsn: 0.778 ± 0.034	3.563GluPro: 3.563 ± 0.084	2.153GluGln: 2.153 ± 0.049	5.268GluArg: 5.268 ± 0.094	2.567GluSer: 2.567 ± 0.063	2.689GluThr: 2.689 ± 0.062	6.001GluVal: 6.001 ± 0.084	0.565GluTrp: 0.565 ± 0.023	0.921GluTyr: 0.921 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	2.662PheAla: 2.662 ± 0.059	0.204PheCys: 0.204 ± 0.015	1.585PheAsp: 1.585 ± 0.041	1.325PheGlu: 1.325 ± 0.04	0.763PhePhe: 0.763 ± 0.032	2.169PheGly: 2.169 ± 0.061	0.487PheHis: 0.487 ± 0.027	0.878PheIle: 0.878 ± 0.035	0.414PheLys: 0.414 ± 0.025	2.265PheLeu: 2.265 ± 0.057	0.439PheMet: 0.439 ± 0.022	0.583PheAsn: 0.583 ± 0.025	1.184PhePro: 1.184 ± 0.043	0.538PheGln: 0.538 ± 0.024	1.345PheArg: 1.345 ± 0.041	1.495PheSer: 1.495 ± 0.039	1.738PheThr: 1.738 ± 0.046	2.0PheVal: 2.0 ± 0.051	0.364PheTrp: 0.364 ± 0.025	0.586PheTyr: 0.586 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	12.509GlyAla: 12.509 ± 0.151	0.771GlyCys: 0.771 ± 0.032	4.382GlyAsp: 4.382 ± 0.073	4.72GlyGlu: 4.72 ± 0.082	2.372GlyPhe: 2.372 ± 0.051	7.94GlyGly: 7.94 ± 0.136	1.857GlyHis: 1.857 ± 0.049	3.133GlyIle: 3.133 ± 0.062	1.56GlyLys: 1.56 ± 0.048	9.614GlyLeu: 9.614 ± 0.119	2.038GlyMet: 2.038 ± 0.047	1.39GlyAsn: 1.39 ± 0.047	5.305GlyPro: 5.305 ± 0.099	3.24GlyGln: 3.24 ± 0.065	7.792GlyArg: 7.792 ± 0.11	5.986GlySer: 5.986 ± 0.094	6.606GlyThr: 6.606 ± 0.106	7.961GlyVal: 7.961 ± 0.104	1.635GlyTrp: 1.635 ± 0.051	2.123GlyTyr: 2.123 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.362HisAla: 2.362 ± 0.05	0.163HisCys: 0.163 ± 0.015	1.34HisAsp: 1.34 ± 0.045	1.279HisGlu: 1.279 ± 0.035	0.359HisPhe: 0.359 ± 0.019	2.009HisGly: 2.009 ± 0.049	0.516HisHis: 0.516 ± 0.025	0.522HisIle: 0.522 ± 0.026	0.24HisLys: 0.24 ± 0.015	2.331HisLeu: 2.331 ± 0.06	0.335HisMet: 0.335 ± 0.018	0.351HisAsn: 0.351 ± 0.018	1.432HisPro: 1.432 ± 0.042	0.441HisGln: 0.441 ± 0.02	1.779HisArg: 1.779 ± 0.044	0.935HisSer: 0.935 ± 0.035	0.946HisThr: 0.946 ± 0.033	1.769HisVal: 1.769 ± 0.051	0.318HisTrp: 0.318 ± 0.019	0.407HisTyr: 0.407 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	4.402IleAla: 4.402 ± 0.083	0.252IleCys: 0.252 ± 0.021	2.629IleAsp: 2.629 ± 0.059	2.406IleGlu: 2.406 ± 0.058	0.711IlePhe: 0.711 ± 0.031	3.212IleGly: 3.212 ± 0.067	0.595IleHis: 0.595 ± 0.025	1.326IleIle: 1.326 ± 0.043	0.645IleLys: 0.645 ± 0.031	2.667IleLeu: 2.667 ± 0.062	0.656IleMet: 0.656 ± 0.028	0.74IleAsn: 0.74 ± 0.033	1.516IlePro: 1.516 ± 0.042	0.745IleGln: 0.745 ± 0.027	1.833IleArg: 1.833 ± 0.05	1.805IleSer: 1.805 ± 0.044	2.16IleThr: 2.16 ± 0.054	3.193IleVal: 3.193 ± 0.067	0.345IleTrp: 0.345 ± 0.024	0.557IleTyr: 0.557 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	2.008LysAla: 2.008 ± 0.058	0.053LysCys: 0.053 ± 0.007	1.046LysAsp: 1.046 ± 0.038	0.901LysGlu: 0.901 ± 0.034	0.264LysPhe: 0.264 ± 0.018	1.247LysGly: 1.247 ± 0.045	0.309LysHis: 0.309 ± 0.019	0.603LysIle: 0.603 ± 0.03	0.479LysLys: 0.479 ± 0.027	0.953LysLeu: 0.953 ± 0.038	0.27LysMet: 0.27 ± 0.017	0.409LysAsn: 0.409 ± 0.023	0.744LysPro: 0.744 ± 0.035	0.434LysGln: 0.434 ± 0.026	1.087LysArg: 1.087 ± 0.041	0.735LysSer: 0.735 ± 0.033	0.917LysThr: 0.917 ± 0.032	1.451LysVal: 1.451 ± 0.046	0.159LysTrp: 0.159 ± 0.012	0.341LysTyr: 0.341 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	15.199LeuAla: 15.199 ± 0.146	0.703LeuCys: 0.703 ± 0.029	6.654LeuAsp: 6.654 ± 0.104	5.754LeuGlu: 5.754 ± 0.097	2.028LeuPhe: 2.028 ± 0.056	9.538LeuGly: 9.538 ± 0.127	1.744LeuHis: 1.744 ± 0.044	3.243LeuIle: 3.243 ± 0.064	1.345LeuLys: 1.345 ± 0.039	10.117LeuLeu: 10.117 ± 0.15	1.758LeuMet: 1.758 ± 0.049	1.528LeuAsn: 1.528 ± 0.045	5.853LeuPro: 5.853 ± 0.082	1.754LeuGln: 1.754 ± 0.045	8.096LeuArg: 8.096 ± 0.107	6.297LeuSer: 6.297 ± 0.08	6.884LeuThr: 6.884 ± 0.087	10.184LeuVal: 10.184 ± 0.139	1.237LeuTrp: 1.237 ± 0.049	1.511LeuTyr: 1.511 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.38MetAla: 2.38 ± 0.047	0.137MetCys: 0.137 ± 0.012	0.937MetAsp: 0.937 ± 0.034	0.878MetGlu: 0.878 ± 0.034	0.406MetPhe: 0.406 ± 0.021	1.594MetGly: 1.594 ± 0.039	0.31MetHis: 0.31 ± 0.017	0.724MetIle: 0.724 ± 0.027	0.325MetLys: 0.325 ± 0.02	1.819MetLeu: 1.819 ± 0.052	0.348MetMet: 0.348 ± 0.022	0.424MetAsn: 0.424 ± 0.022	1.134MetPro: 1.134 ± 0.041	0.326MetGln: 0.326 ± 0.022	1.564MetArg: 1.564 ± 0.045	1.719MetSer: 1.719 ± 0.043	1.912MetThr: 1.912 ± 0.043	1.559MetVal: 1.559 ± 0.046	0.221MetTrp: 0.221 ± 0.016	0.271MetTyr: 0.271 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	1.953AsnAla: 1.953 ± 0.049	0.11AsnCys: 0.11 ± 0.011	0.941AsnAsp: 0.941 ± 0.035	0.863AsnGlu: 0.863 ± 0.029	0.368AsnPhe: 0.368 ± 0.024	1.496AsnGly: 1.496 ± 0.046	0.361AsnHis: 0.361 ± 0.02	0.624AsnIle: 0.624 ± 0.027	0.302AsnLys: 0.302 ± 0.019	1.707AsnLeu: 1.707 ± 0.051	0.276AsnMet: 0.276 ± 0.017	0.404AsnAsn: 0.404 ± 0.03	1.317AsnPro: 1.317 ± 0.042	0.439AsnGln: 0.439 ± 0.023	1.176AsnArg: 1.176 ± 0.035	0.777AsnSer: 0.777 ± 0.031	0.958AsnThr: 0.958 ± 0.045	1.269AsnVal: 1.269 ± 0.038	0.223AsnTrp: 0.223 ± 0.019	0.391AsnTyr: 0.391 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	8.848ProAla: 8.848 ± 0.15	0.35ProCys: 0.35 ± 0.021	3.811ProAsp: 3.811 ± 0.073	4.494ProGlu: 4.494 ± 0.104	1.329ProPhe: 1.329 ± 0.043	7.005ProGly: 7.005 ± 0.113	1.133ProHis: 1.133 ± 0.041	1.242ProIle: 1.242 ± 0.036	0.655ProLys: 0.655 ± 0.033	4.694ProLeu: 4.694 ± 0.08	0.995ProMet: 0.995 ± 0.028	0.733ProAsn: 0.733 ± 0.03	2.725ProPro: 2.725 ± 0.076	1.898ProGln: 1.898 ± 0.051	4.035ProArg: 4.035 ± 0.083	3.868ProSer: 3.868 ± 0.085	3.837ProThr: 3.837 ± 0.091	6.125ProVal: 6.125 ± 0.098	0.994ProTrp: 0.994 ± 0.033	1.067ProTyr: 1.067 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.719GlnAla: 4.719 ± 0.075	0.168GlnCys: 0.168 ± 0.014	1.928GlnAsp: 1.928 ± 0.054	1.801GlnGlu: 1.801 ± 0.046	0.554GlnPhe: 0.554 ± 0.026	2.722GlnGly: 2.722 ± 0.066	0.501GlnHis: 0.501 ± 0.021	1.058GlnIle: 1.058 ± 0.039	0.364GlnLys: 0.364 ± 0.02	2.257GlnLeu: 2.257 ± 0.055	0.633GlnMet: 0.633 ± 0.025	0.397GlnAsn: 0.397 ± 0.02	1.518GlnPro: 1.518 ± 0.056	0.842GlnGln: 0.842 ± 0.037	2.209GlnArg: 2.209 ± 0.05	1.211GlnSer: 1.211 ± 0.035	1.543GlnThr: 1.543 ± 0.045	3.107GlnVal: 3.107 ± 0.064	0.495GlnTrp: 0.495 ± 0.024	0.512GlnTyr: 0.512 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	10.673ArgAla: 10.673 ± 0.151	0.564ArgCys: 0.564 ± 0.028	3.894ArgAsp: 3.894 ± 0.071	4.367ArgGlu: 4.367 ± 0.08	1.921ArgPhe: 1.921 ± 0.052	5.915ArgGly: 5.915 ± 0.085	1.796ArgHis: 1.796 ± 0.044	2.696ArgIle: 2.696 ± 0.059	1.085ArgLys: 1.085 ± 0.039	8.45ArgLeu: 8.45 ± 0.123	1.712ArgMet: 1.712 ± 0.05	1.033ArgAsn: 1.033 ± 0.037	4.748ArgPro: 4.748 ± 0.092	2.346ArgGln: 2.346 ± 0.057	8.295ArgArg: 8.295 ± 0.148	4.814ArgSer: 4.814 ± 0.078	5.08ArgThr: 5.08 ± 0.07	6.434ArgVal: 6.434 ± 0.094	1.28ArgTrp: 1.28 ± 0.036	1.601ArgTyr: 1.601 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	7.943SerAla: 7.943 ± 0.137	0.526SerCys: 0.526 ± 0.024	2.848SerAsp: 2.848 ± 0.064	2.757SerGlu: 2.757 ± 0.059	1.676SerPhe: 1.676 ± 0.042	6.101SerGly: 6.101 ± 0.1	1.113SerHis: 1.113 ± 0.035	1.83SerIle: 1.83 ± 0.05	0.821SerLys: 0.821 ± 0.03	5.685SerLeu: 5.685 ± 0.083	1.257SerMet: 1.257 ± 0.039	0.892SerAsn: 0.892 ± 0.031	3.886SerPro: 3.886 ± 0.086	1.97SerGln: 1.97 ± 0.046	4.601SerArg: 4.601 ± 0.085	4.262SerSer: 4.262 ± 0.09	4.305SerThr: 4.305 ± 0.074	4.955SerVal: 4.955 ± 0.08	1.123SerTrp: 1.123 ± 0.037	1.27SerTyr: 1.27 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	8.419ThrAla: 8.419 ± 0.122	0.547ThrCys: 0.547 ± 0.026	3.6ThrAsp: 3.6 ± 0.072	3.202ThrGlu: 3.202 ± 0.07	1.526ThrPhe: 1.526 ± 0.049	6.784ThrGly: 6.784 ± 0.111	1.125ThrHis: 1.125 ± 0.04	2.452ThrIle: 2.452 ± 0.055	0.833ThrLys: 0.833 ± 0.034	5.489ThrLeu: 5.489 ± 0.086	1.141ThrMet: 1.141 ± 0.039	1.092ThrAsn: 1.092 ± 0.038	4.027ThrPro: 4.027 ± 0.073	1.589ThrGln: 1.589 ± 0.048	4.148ThrArg: 4.148 ± 0.072	4.014ThrSer: 4.014 ± 0.077	4.835ThrThr: 4.835 ± 0.107	6.447ThrVal: 6.447 ± 0.129	1.174ThrTrp: 1.174 ± 0.039	1.358ThrTyr: 1.358 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	11.7ValAla: 11.7 ± 0.132	0.821ValCys: 0.821 ± 0.028	5.764ValAsp: 5.764 ± 0.082	5.83ValGlu: 5.83 ± 0.088	2.244ValPhe: 2.244 ± 0.061	7.459ValGly: 7.459 ± 0.094	1.745ValHis: 1.745 ± 0.047	3.367ValIle: 3.367 ± 0.068	1.297ValLys: 1.297 ± 0.041	11.175ValLeu: 11.175 ± 0.142	1.706ValMet: 1.706 ± 0.05	1.584ValAsn: 1.584 ± 0.044	5.776ValPro: 5.776 ± 0.093	1.913ValGln: 1.913 ± 0.044	6.701ValArg: 6.701 ± 0.1	5.749ValSer: 5.749 ± 0.092	6.004ValThr: 6.004 ± 0.109	10.378ValVal: 10.378 ± 0.136	1.102ValTrp: 1.102 ± 0.033	1.593ValTyr: 1.593 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.693TrpAla: 1.693 ± 0.047	0.16TrpCys: 0.16 ± 0.014	0.891TrpAsp: 0.891 ± 0.03	0.8TrpGlu: 0.8 ± 0.029	0.469TrpPhe: 0.469 ± 0.025	1.117TrpGly: 1.117 ± 0.041	0.306TrpHis: 0.306 ± 0.019	0.515TrpIle: 0.515 ± 0.024	0.231TrpLys: 0.231 ± 0.017	1.63TrpLeu: 1.63 ± 0.045	0.35TrpMet: 0.35 ± 0.019	0.318TrpAsn: 0.318 ± 0.02	0.71TrpPro: 0.71 ± 0.029	0.496TrpGln: 0.496 ± 0.024	1.289TrpArg: 1.289 ± 0.044	1.013TrpSer: 1.013 ± 0.038	0.996TrpThr: 0.996 ± 0.031	1.305TrpVal: 1.305 ± 0.042	0.353TrpTrp: 0.353 ± 0.021	0.329TrpTyr: 0.329 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.226TyrAla: 2.226 ± 0.055	0.157TyrCys: 0.157 ± 0.014	1.23TyrAsp: 1.23 ± 0.037	1.168TyrGlu: 1.168 ± 0.04	0.551TyrPhe: 0.551 ± 0.029	1.801TyrGly: 1.801 ± 0.048	0.371TyrHis: 0.371 ± 0.022	0.498TyrIle: 0.498 ± 0.026	0.279TyrLys: 0.279 ± 0.016	2.055TyrLeu: 2.055 ± 0.053	0.309TyrMet: 0.309 ± 0.021	0.424TyrAsn: 0.424 ± 0.026	1.058TyrPro: 1.058 ± 0.038	0.548TyrGln: 0.548 ± 0.031	1.503TyrArg: 1.503 ± 0.045	1.175TyrSer: 1.175 ± 0.042	1.218TyrThr: 1.218 ± 0.041	1.668TyrVal: 1.668 ± 0.042	0.291TyrTrp: 0.291 ± 0.021	0.481TyrTyr: 0.481 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2533 proteins (897614 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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