Bacillus akibai (strain ATCC 43226 / DSM 21942 / JCM 9157 / 1139)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus akibai

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4QPL1|W4QPL1_BACA3 Serine-type D-Ala-D-Ala carboxypeptidase OS=Bacillus akibai (strain ATCC 43226 / DSM 21942 / JCM 9157 / 1139) OX=1236973 GN=JCM9157_878 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.35VSYY6 pKa = 11.29LMMLALALMLVLAACGGAADD26 pKa = 5.28PDD28 pKa = 4.18PAPADD33 pKa = 3.7DD34 pKa = 4.54AEE36 pKa = 4.52EE37 pKa = 4.19TGEE40 pKa = 4.02EE41 pKa = 4.33TEE43 pKa = 4.49AEE45 pKa = 4.18GGEE48 pKa = 4.0ATTRR52 pKa = 11.84EE53 pKa = 4.17FTDD56 pKa = 3.06ITVMTGGEE64 pKa = 3.91QGTYY68 pKa = 10.15FPLGAALANNIINVHH83 pKa = 5.08VEE85 pKa = 4.04NVDD88 pKa = 3.36AASFSSGASVANINSLVDD106 pKa = 3.57GDD108 pKa = 4.69ADD110 pKa = 4.06LALVQNDD117 pKa = 3.47VAFFASEE124 pKa = 4.52GINMFEE130 pKa = 4.08EE131 pKa = 4.7AGALEE136 pKa = 4.63GFQGIATLYY145 pKa = 9.98PEE147 pKa = 5.08VIQIITAADD156 pKa = 3.39SGIEE160 pKa = 4.1TVEE163 pKa = 4.0DD164 pKa = 3.66LVGKK168 pKa = 10.16RR169 pKa = 11.84VAVGDD174 pKa = 4.13LGSGAEE180 pKa = 3.9INARR184 pKa = 11.84QILEE188 pKa = 4.14VHH190 pKa = 6.22GLSYY194 pKa = 11.51DD195 pKa = 4.47DD196 pKa = 4.2ISEE199 pKa = 4.46EE200 pKa = 4.11YY201 pKa = 10.14MSFGDD206 pKa = 3.85AAGSIQDD213 pKa = 3.99GNIDD217 pKa = 3.62AAFITAGLPTGAVEE231 pKa = 4.08ALKK234 pKa = 9.43ATKK237 pKa = 9.91EE238 pKa = 3.73ISLVSISAEE247 pKa = 4.01KK248 pKa = 10.13IAEE251 pKa = 3.98LSAAYY256 pKa = 8.74PYY258 pKa = 9.08YY259 pKa = 10.84TEE261 pKa = 4.07FTVPADD267 pKa = 3.71AYY269 pKa = 9.24GTAGDD274 pKa = 3.89TTTAAVLAMLAVSADD289 pKa = 3.31MDD291 pKa = 3.96EE292 pKa = 4.71DD293 pKa = 3.81LVYY296 pKa = 10.79DD297 pKa = 3.3ITKK300 pKa = 11.02AMFEE304 pKa = 4.14NTDD307 pKa = 3.28AFSNAHH313 pKa = 4.55QQGNNITLEE322 pKa = 4.09SALDD326 pKa = 3.65GMSIPVHH333 pKa = 6.53PGAQRR338 pKa = 11.84YY339 pKa = 8.74YY340 pKa = 11.03DD341 pKa = 3.72EE342 pKa = 4.21QQ343 pKa = 5.07

Molecular weight: 35.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4QT33|W4QT33_BACA3 Maltodextrin-binding protein OS=Bacillus akibai (strain ATCC 43226 / DSM 21942 / JCM 9157 / 1139) OX=1236973 GN=JCM9157_2361 PE=3 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4710

0

4710

1307598

37

2162

277.6

31.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.742 ± 0.042

0.708 ± 0.012

5.019 ± 0.029

7.749 ± 0.039

4.629 ± 0.03

6.654 ± 0.037

2.145 ± 0.018

7.794 ± 0.035

6.531 ± 0.039

9.998 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.015

4.438 ± 0.028

3.524 ± 0.022

4.008 ± 0.03

4.084 ± 0.028

5.996 ± 0.026

5.55 ± 0.024

7.184 ± 0.033

1.072 ± 0.014

3.462 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski